Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,475	C→T	R302R (CGG→CGA)	dcp ←	peptidyl‑dipeptidase Dcp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,475	0	C	T	93.8%	37.4 / ‑5.5	17	R302R (CGG→CGA)	dcp	peptidyl‑dipeptidase Dcp
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (14/1);  total (16/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCCC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGT  >  NC_000913/1626247‑1626708                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           gAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGAGCCTCATCAAGATAAAATTTTTCCCGCCGTACCTGTTCCGCCTAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAAACCGCCCTGCTGCTTATCGATAACAGCCTGTATGGAGGATAAATCATAGCTCGCACATTGAAGCGCCCCC‑GGAGACA‑TTTCAC‑TCATCACGTTAAGTGCTGCtt                                                                                                                                                                                                                     >  2:239201/1‑251 (MQ=255)  aaGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGATGCttt                                                                                                                                                                                                                    >  1:563001/1‑250 (MQ=255)        tGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGCCGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGGGGTGCTTCAGgtgt                                                                                                                                                                                                              >  1:43125/1‑251 (MQ=255)           ttCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATAAAATTTTTCCCGAAGTACCTGTTTAGCAAAAAAGTAACC‑GTACCAAGGGTGCGCGCTAAACCCGACCTGCTGATTATCGCAAACCACCCGATTGGGGGCTAATTAATAGCTCGCACGTTGCCGCGCCGCA‑GGA‑ACAATTTATC‑GCATAAAGTTAGGGGCTGATTCAGGGGtttt                                                                                                                                                                                                           >  2:416093/1‑251 (MQ=255)                      gAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAACTGCGACCCAAAAAGATCACATTTCTCCCGCCGTACATGTTCAGCATAAAA‑ATCCCAGTCCCACGGCTGCGCCCTAAACCAGCACTGCTGCTTATCCATAAACGCCTGTATGAAAGCTAAATCATCGCCCGCACGTGGTAGCGCCGCTCGGA‑ACA‑TTTCCTTGCA‑AAATTAAAGTGATTCTTTAGGTGTTTTTGCCATCTgtt                                                                                                                                                                                                >  1:227108/1‑249 (MQ=255)                         tACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGAGCTGCTTCAGGTGTTTTTGCCATCTGATCgg                                                                                                                                                                                             >  1:232586/1‑251 (MQ=255)                                              gtTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAAACGCCTGTATGGAGGCTAATTCATAGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATgaggactaa                                                                                                                                                                         >  1:430553/1‑242 (MQ=255)                                                                                     aTCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGAtct                                                                                                                                 >  1:395551/1‑251 (MQ=255)                                                                                                 ccgccgTACCTGTTCAGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTCCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGGTGACGCACCGCT‑GGA‑ACCATTTCTC‑GCCTAAAGTTAAGTGCTGCTTCAGGAGTTTTTGCCATCTGATCGCCGATTTTCCATGCGGCATAATGATGAAACCCACACAGTGTTTCCtgttaggcccggttctccatcatacg                                                                                                                       >  2:68952/1‑227 (MQ=255)                                                                                                         ccTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGAACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGAAGTTGAATGAtc                                                                                                             >  1:4573/1‑250 (MQ=255)                                                                                                                      aaa‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCAt                                                                                                >  1:543124/1‑251 (MQ=255)                                                                                                                           gCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATAGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATAATTGGc                                                                                            >  1:566228/1‑251 (MQ=255)                                                                                                                                                    aaccGCCCTGCTGCTTATCGATAACCGCCTGTATGGCGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATATCCACCAGACGTTGAATGCTAGCGCGGGTATCATTGGCATCATTTTTTTCCGCCCGCGTcc                                                                     >  2:333026/3‑249 (MQ=255)                                                                                                                                                                               ccTGTATGGAGGCTACTTCATCGATCGCACGATGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGATAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGCACACCACTTCGTGTTGCCTGTTGTGCACGGATCTCCTCCAGAAGTTGAATGCGAGCGCGGGTATCATTGGCATCATTTTTTTACGCTCGCGTCCAGCCCGCACTACACAGTTTTTCaaccgtc                                        >  2:63868/1‑245 (MQ=255)                                                                                                                                                                                   tagggaggtcaaTTCATCGCTCGCACGGTGACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGAGCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCa                                     <  2:232586/240‑1 (MQ=255)                                                                                                                                                                                                                gACGCGCCGCT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGgttgt       >  2:343050/1‑251 (MQ=255)                                                                                                                                                                                                                     gccgcT‑GGA‑ACAATTTCTC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCACGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGggt  >  1:491272/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           GAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAA‑TGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCT‑GGA‑ACAATTTCCC‑GCATAAAGTTAAGTGCTGCTTCAGGTGTTTTTGCCATCTGATCGGCGATTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGT  >  NC_000913/1626247‑1626708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑