Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,597,364	T→C	intergenic (‑4922/+1253)	hipB ← / ← lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,597,364	0	T	C	100.0%	74.8 / NA	23	intergenic (‑4922/+1253)	hipB/lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/15);  total (8/15)

CCTGAACGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAA  >  NC_000913/1597120‑1597589                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  ccTGAACGCCCTCCAATGAATTTTCTTTGTTAATAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGGCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGaa                                                                                                                                                                                                                             <  2:473510/251‑1 (MQ=255)  cTGAACGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGTTTACCAATTTGAAATTCAATCGAGGAAt                                                                                                                                                                                                                            >  1:15037/1‑251 (MQ=255)    gAACGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATc                                                                                                                                                                                                                           >  1:565960/1‑250 (MQ=255)          ccaccaATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTAttt                                                                                                                                                                                                                    <  1:482200/251‑1 (MQ=255)          ccaccaATGAATATTCGTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTAttt                                                                                                                                                                                                                    <  1:249159/251‑1 (MQ=255)               aaTGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTttat                                                                                                                                                                                                               >  1:407546/1‑251 (MQ=255)                 tGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTttatt                                                                                                                                                                                                              >  1:251920/1‑250 (MQ=255)                                         tCCATTATTGGAAATCTGGAATGTAACATAACCGACATATCCATTATAGAGTCTGCGTAACGTGCTGCCAGCGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCAGTAACACACCCCACCTCGCGACTAGTCGCCTCCCCAGTTCCTTGGTTACCAATTTGCACTTCAATCGGGGGATCATTTCTTTTTATTAGCCACTCGCCACCCTTTTCAAcac                                                                                                                                                                                     >  2:560776/1‑249 (MQ=255)                                                               gtgCCATTACCGAAATATCCATTATAGAGTCTGCGGACCGTGATTACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGGGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTGTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGccc                                                                                                                                                                 <  1:370881/249‑1 (MQ=255)                                                                  ccATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTg                                                                                                                                                            <  1:195472/251‑1 (MQ=255)                                                                                              tGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTc                                                                                                                                >  1:285123/1‑251 (MQ=255)                                                                                                cGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGt                                                                                                                              <  1:546010/251‑1 (MQ=255)                                                                                                                agTCTTGTTCATGCACATTCCGGGTTCCGATACCTGTGGCATTGCCACCGATAAGCGTACTTTCAGCCGTAACCAACCCCCCCTCGCGATTAGTCGACTCCCCAGTTCTTTTATTACCAATTTGAAATTCTATCGAGCAATCATTATTTTTTATTAGCCACTCGCCACCCTTTGCAACGACACCCGGGACATTACTGCCCGCCTGATATCCTAAAATGGCTACGATTGAACTCGTGActtatcccttatcc                                                                                                              <  2:394582/251‑1 (MQ=255)                                                                                                                         ccTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCAACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATAttt                                                                                                     <  1:177462/251‑1 (MQ=255)                                                                                                                                    aGGGTTCCGATACCGGTGGCATTGCCTCCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCAGTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCtgt                                                                                          <  1:156085/251‑1 (MQ=255)                                                                                                                                                           gccaccgttgaccgtagttgcAGCCGTAACCAACCCCCCCTCGCAAATCGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATGCAAGCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACGCGTGACGTAACCCTTATCCGGAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGaata                                                                   <  2:65799/231‑1 (MQ=255)                                                                                                                                                                        gTATTTTCAGCCGAAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGATACCTATTTCGAATAATTCGGTCGTCAg                                                      <  2:407546/251‑1 (MQ=255)                                                                                                                                                                                          aaccCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGcc                                                                                                                                                              >  1:164320/1‑129 (MQ=255)                                                                                                                                                                                          aaccCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAACCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGcc                                                                                                                                                              <  2:164320/129‑1 (MQ=255)                                                                                                                                                                                            cccccccTCGCGAATAGTAGCCTCCCCAGTTCCTTGATTACCAATTTGAACTTCAATCGAGGCATCCTTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATTGCCTTAAATGGCGACTATTGACCTCGTGCCTTCACCCTTTTCCGTAATATTTTTTGAACTTGTGCCATCGCTACCTATTTCGAATAATTCGTTCTTCAGACCAGCGTCCTCTCCCTCa                                   >  2:471609/1‑250 (MQ=255)                                                                                                                                                                                             ccccctTCGCGAATAGTAGCCTCCCCAGTTCCTTGATTACCAATTTTAAATGAGATCGAGGAATTATTATTTTTTATTAGCCACTCGCCTCCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCGGTGCCATAGCTATCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCtctctcacaac                                 <  2:226978/251‑8 (MQ=255)                                                                                                                                                                                                                      cAGTTCCTTGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCGCAACATTGACCGTCCCGACGCCTCCTGTc        <  1:438964/251‑1 (MQ=255)                                                                                                                                                                                                                              tGATTACCAATTTGAAATTCAATCGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACTTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGACAGAACAGAGTCCTCTCCCTCAACATTGACCGGCCCGACGCCTCCTGTCgaagaa  <  1:560776/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  CCTGAACGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGATAGCCTAAAATGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAA  >  NC_000913/1597120‑1597589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑