Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,587,323	C→T	A138T (GCC→ACC)	ydeQ ←	putative fimbrial adhesin protein YdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,587,323	0	C	T	100.0%	63.5 / NA	23	A138T (GCC→ACC)	ydeQ	putative fimbrial adhesin protein YdeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

GAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGCCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCGCCGTAATCATTCCAGCAACTTATATG  >  NC_000913/1587082‑1587548                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  gagaGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAAc                                                                                                                                                                                                                          <  1:312445/251‑1 (MQ=255)     gCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTggg                                                                                                                                                                                                                      >  1:487538/1‑251 (MQ=255)           gTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTgg                                                                                                                                                                                                                       >  1:269206/1‑244 (MQ=255)           gTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTgg                                                                                                                                                                                                                       <  2:269206/244‑1 (MQ=255)               gCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAatat                                                                                                                                                                                                               >  1:419578/1‑248 (MQ=255)                   gAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGtt                                                                                                                                                                                                         <  1:29305/250‑1 (MQ=255)                                cAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGc                                                                                                                                                                                           <  1:536096/251‑1 (MQ=255)                                                                  gagaTCCACGGTGACATTTCGACTATCAACGGCGCAGCCGCCTGTTGGCATAACAAAATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGAAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCACCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTgg                                                                                                                                                         >  2:507757/1‑251 (MQ=255)                                                                                                 gTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAACTAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCACTTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATCTAGAGTTTTAACGGTACTGGCATTGGTGTCTTATCCCCACTATACAATACGTTGGTCTTTgtcgttcccgggacgggtcaggtcac                                                                                                                          >  2:275103/1‑230 (MQ=255)                                                                                                           cTGTTGGCATAACAACATTTTTATTTGAAATAATATTCCACGTCAAATTACGCGGATTTCCGATTCCTAATGTTGCAATTTTATACATATGAAGCAGGGCAATGACTTCCCCCGCTGTTATGACAACTCAACCGGTCGCCCAAGATGAGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGCCTTACCACCAATATCCAATACGTTGGTATTTTTGTTCAGCGGGCAGGGGTAGGTCACatactgcc                                                                                                                  >  2:162164/1‑243 (MQ=255)                                                                                                            tGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCACCGGGAAGGGGTAGGTCACATTATTCCAGt                                                                                                               >  1:89954/1‑251 (MQ=255)                                                                                                                       aaacaGTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGATTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCt                                                                                                    <  1:185752/250‑1 (MQ=255)                                                                                                                                                ccACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACAAATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGa                                                                           <  1:432721/251‑1 (MQ=255)                                                                                                                                                  aCGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGaa                                                                          >  1:479921/1‑250 (MQ=255)                                                                                                                                                    gTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGc                                                                       >  1:438807/1‑251 (MQ=255)                                                                                                                                                      aaaaTTACGCCGTATTCCGCTTCCTAATGGTGCAATTTTATACATAGGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACGGGGGTAATATAGAGTTTTAACGGTAATTGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATTACTGTAGGGAGCCTGCGAAAGCt                                                                      <  2:515749/250‑1 (MQ=255)                                                                                                                                                                           tCCTAATGTTGCAATTTTATATATATGAAAACGGTCAATGACTTCCCCCGCTTTTATTACTAATTCACCGGTCGCTCCATCTTGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTTTTATCACCAATATCCAATCCGTTTGTATTTTTCGTCAACTGTAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTTGGGAGCCTGCGAAAGTTGAGCCTTTTACCAGGTTtata                                                 <  2:453257/250‑1 (MQ=255)                                                                                                                                                                           tCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTtat                                                  <  1:275103/249‑1 (MQ=255)                                                                                                                                                                               aaTGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGa                                              <  1:15227/249‑1 (MQ=255)                                                                                                                                                                                                      aaaaCGGGCAATGACTTCCCCCGTTTTTATGACATCTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTGGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGCCTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCGCCGTAAt                     <  2:419578/248‑1 (MQ=255)                                                                                                                                                                                                           gggCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCGCCGTAATCATTc                >  1:440659/1‑251 (MQ=255)                                                                                                                                                                                                                        ccccGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATCTAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATCTGATCAGTGTCGTACCAGCCGCCGTAATCATTCCAGAAACTTatat   >  2:535338/1‑251 (MQ=255)                                                                                                                                                                                                                         cccGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCGCCGTAATCATTCCAGCAACTTATATg  >  1:467143/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  GAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGCCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCGCCGTAATCATTCCAGCAACTTATATG  >  NC_000913/1587082‑1587548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑