Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,514,985	C→T	A75V (GCG→GTG)	ydcV →	putative ABC transporter membrane subunit YdcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,514,985	0	C	T	96.7%	68.1 / NA	31	A75V (GCG→GTG)	ydcV	putative ABC transporter membrane subunit YdcV
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (14/15);  minor base G (1/0);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTC  >  NC_000913/1514740‑1515230                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACAt                                                                                                                                                                                                                                                  <  1:360436/251‑1 (MQ=255)                      gATGCACTCTGAACGCGCACCGTTTTTCATCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGCCATTAATTGCGCTGGTGTTAgggg                                                                                                                                                                                                                             >  1:506518/1‑250 (MQ=255)                                gaccgcgCACCGTTTTTCAGCAAACTGGCGGCCTGGGGCGGCGTTGTGTTCCTGCATTTTCCCATCCGTATAATCGCCGCCTAGTCGTTTAACGCTGAAGTGGCGGCGTTTAGTTTGCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTTTCACTTAGAGTGGGGGCGCTGGTGACATTAATTGCGGTGGTGTTAGGGACGCTGGCa                                                                                                                                                                                                                     <  2:291158/246‑1 (MQ=255)                                          cGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGGGGCATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCCCtg                                                                                                                                                                                                         >  1:65787/1‑251 (MQ=255)                                          cGTTTTACCTCAATCGGGCTGCTTGGGGCGGCGTTGTTTTCCTACAGTTTCCCATCCTGATAACCGCCGCCTATGCGTTTAACTCTGAAGATGCGGCGTGTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTGGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCtg                                                                                                                                                                                                         <  2:404417/251‑1 (MQ=255)                                                 ccTCAAACTGGCGGCCGGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCGGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgac                                                                                                                                                                                                  <  2:194544/251‑1 (MQ=255)                                                    cAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgacg                                                                                                                                                                                                 <  1:45530/249‑1 (MQ=255)                                                    cAAACGGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTTTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgacga                                                                                                                                                                                                <  1:549270/250‑1 (MQ=255)                                                                                             ccATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGGGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTActgctgctg                                                                                                                                                      >  1:478126/1‑251 (MQ=255)                                                                                                       aaTCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACCCTGGCAGCTGCCGCGCCGTGGCGGCCAGAATTTTTCGGCAAAAACGCCCTTTCGCTGTTACTGCTGCTGCCCATTtcgc                                                                                                                                            >  2:238666/1‑251 (MQ=255)                                                                                                           gccgccTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGcc                                                                                                                                        <  1:480177/251‑1 (MQ=255)                                                                                                                     cGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGGCGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCCTTGTc                                                                                                                              >  1:194851/1‑251 (MQ=255)                                                                                                                           acacTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGACTGACGCTGCGCTGGTTTAGCGAGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGGTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTCCTGCCCATTGCGCTGCCGGGCATTGTCACTggg                                                                                                                        >  1:459155/1‑250 (MQ=255)                                                                                                                            cacTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGAGCTGGTTTAGCGTGGGAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTc                                                                                                                       <  1:249388/251‑1 (MQ=255)                                                                                                                                           gTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGGGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCg                                                                                                        >  1:487446/1‑251 (MQ=255)                                                                                                                                                       aCCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGCTATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGGCATTAAATGCGCCGGTGTTAGGGACGCTGGCAGCGGCCGAGCTGGGGCGACGCGACTTTTTCTGCAAAAACGCCATTTCGCTGTTATTGCTGCCGCCCATTTCGCTGCCGGGCATTGTCACTGTTCTGGCGTTATGAACCGCCTTTAAAAccc                                                                                            >  2:162930/1‑250 (MQ=255)                                                                                                                                                          gCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGGTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATc                                                                                          >  1:307330/1‑250 (MQ=255)                                                                                                                                                              ggCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTTATTGCGCTGGTGTTAGGGACGCGGGCAGCTGCCGCGCTGTGGCGACGAGCATTTTTCGGCCAAAACACCATTTCGCTGTTAATGCTGCTGCCCATTGCGCTGCCGGGCCTTGGCACTGTTCCTGCGTTTTTAACCGCCTTTAAAACCAtaccact                                                                                     >  2:92045/1‑245 (MQ=255)                                                                                                                                                                   gACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGc                                                                                >  1:425690/1‑251 (MQ=255)                                                                                                                                                                         gcgcttGTTTAGCTTGGCAGCACAGCGTAGTGATATGCTTTATGCCGTGACCTTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATCGCGCTGTTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTCCGGCAAAAACGCCATTTCGCTGTTCCGTCTGCTGCCCATTGCGTTTCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGAt                                                                          <  1:296527/251‑1 (MQ=255)                                                                                                                                                                               gTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCTTTTCGCCGTTACTGCTGCTCCCCATTGCGCGGCCGGGCATTGTAACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGGGCCGGGATTTTTcc                                                                    >  1:489020/1‑250 (MQ=255)                                                                                                                                                                                                  cGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATAAATTTGGAGCCGGGATTTTTCACCATCGTGGTTGGTCATGc                                                 >  1:83982/1‑251 (MQ=255)                                                                                                                                                                                                        gATATTCTTGATGCCGTGACACTGTCCCTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGAAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACttt                                           <  1:12868/251‑1 (MQ=255)                                                                                                                                                                                                                tGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTa                                    <  1:238666/250‑1 (MQ=255)                                                                                                                                                                                                                 gATGCCGTGACACTGTCACTTAAAGTGGCGGCGCGGGTGACATTAATTGCGCTGGGGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGt                                  <  1:278129/251‑1 (MQ=255)                                                                                                                                                                                                                         gtgtcggtcccTTAAAGGGGCGGCGCTGGTGACATTAATTGCGTTGGTGTGAGGGACGCTGGCAGCTGCCGCGTTGGGGCGACGAGTCTTTTTCGGAAAAAACCCCATTTCTCTGTTCCTGCTGCTCCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTACCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGgtgt                          <  2:13942/241‑1 (MQ=255)                                                                                                                                                                                                                             ctgcctcgtAAAGTGGCGGGGCTGGTGTCATTAATGTAGTGGGTGTTAGTGCAGCTGGCCGCTGCTGCGTTGTGGGGCCGTGATTTTTGCGGAAAAACCGCCATTCCGCTGGTATTGCTGCTTCCCATTGCGCTGCCGGGCATGGTCCCTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGttt                        <  2:559006/241‑1 (MQ=255)                                                                                                                                                                                                                                     tAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCCGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCCATCTGGAGCCGGGATTTTTCACCCTCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAAAAATGTc               >  1:67332/1‑250 (MQ=255)                                                                                                                                                                                                                                        aggggcggcGCTGGTGACATAAATGGCGCTGGTGTTAGGGACGCGGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGGCGGGATTTTTCTCCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCg           <  1:238383/248‑1 (MQ=255)                                                                                                                                                                                                                                         gTGGCGGAGCTGGTGACATTAATTGCGCTGGGGGTAGGGACCCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAAAGCCATTTAGCTGTTACCGCTGCTGCCCATTGCGCTGCCGGGCATTGTCCCTGGTTTGGCGGTATTAACCGCCTTTAAAACCATCAATCTGGAGACGGGTTTTTTCACCCTCCTGGTCGTTCATGCTGCTTTTTGTGTAGTtgggtgggttaccaatttcctcgc          >  2:501023/1‑229 (MQ=255)                                                                                                                                                                                                                                                 gcTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGTGGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAACCGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCTCTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCAGCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTc  <  1:49740/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GTGAAACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTC  >  NC_000913/1514740‑1515230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑