Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,399,590	IS5 (+) +4 bp	intergenic (‑64/+128)	fnr ← / ← ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1399590 =	0 (0.000)	22 (0.430)	21/484	0.9	100%	intergenic (‑64/+131)	fnr/ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1399593	0 (0.000)	42 (0.830)	41/482	0.2	100%	intergenic (‑67/+128)	fnr/ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGC  >  NC_000913/2101503‑2101966                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          aaaaaaaaCTACGATTGTCATGATGAGCATAATGTAGCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATAGCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCATCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaag                                                                                                                                                                                                                       <  1:69707/251‑1 (MQ=255)                    atgatgAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACAAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATACATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGAAACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGAGGGGaa                                                                                                                                                                                                     >  2:96179/1‑250 (MQ=255)                        ggaGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCGTCACAGGACATAAAGTATCCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCCCCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAttct                                                                                                                                                                                                <  2:334179/250‑1 (MQ=255)                            cATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATGCTGCGCACCAATCAACAACAGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                                                                                                            >  2:155360/1‑251 (MQ=255)                                      cGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACTCAAAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCGGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTGGCTGCTGACTCa                                                                                                                                                                                  <  2:563405/251‑1 (MQ=255)                                        cATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGt                                                                                                                                                                                <  1:486119/251‑1 (MQ=255)                                                    gAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttct                                                                                                                                                                    >  1:176688/1‑251 (MQ=255)                                                                       acCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCTGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                                                                                                                                                 <  1:358387/251‑1 (MQ=255)                                                                               ggCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCg                                                                                                                                         >  1:368052/1‑251 (MQ=255)                                                                                 cTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAAt                                                                                                                                                                                                               <  1:330020/179‑1 (MQ=255)                                                                                 cTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAAt                                                                                                                                                                                                               >  2:330020/1‑179 (MQ=255)                                                                                                         tCTTCACAGTACAGAATACAAGCTTGATCGAATCCATTTACACTCACAAAATCTGAAAAACGTACCAGCATAAATGATCCGGCGCACCGATCAACAACAGTATCAGAGGAGATACTTTCTTTAGGAATTTTTGTTTAATTAAGGCAGGTGCTAATAAGCGGGGCACTTCTTCTCGGCTGACTCGTTCATTTCATTTCTTCCTGTGTGAGCCATTTTTTTATCCCGTAAAtacattggatcgggctattt                                                                                                                 >  2:397032/1‑230 (MQ=255)                                                                                                                tgTACATAAAGAAACATGGAACGAAGCCATTTACACGCAAAAAATCTGAAAAACGTACCAGCATAAAAGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGACAGGTGCGAATAAGCGGGGAAATTCTTCTCGCCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTATCCCGTAACTGCCTTGAATCAGCATATTTAGACCGttt                                                                                                        >  2:26857/2‑251 (MQ=25)                                                                                                                       aaaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc                                                                                                  <  1:97036/250‑1 (MQ=255)                                                                                                                           tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc                                                                                                  >  1:555151/1‑246 (MQ=255)                                                                                                                           tAACCTTGATCGAAGCCATGTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATTAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc                                                                                                  <  2:555151/246‑1 (MQ=255)                                                                                                                             aCCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa                                                                                           <  1:197630/251‑1 (MQ=255)                                                                                                                                 tGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg                                                                                         <  2:404395/249‑1 (MQ=255)                                                                                                                                                   cGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATCAATACTTTCTTTAGGAATTTTTGTTTTATTAAGGCAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCATCCTATTTTGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                     >  2:519630/1‑251 (MQ=255)                                                                                                                                                   cGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                     <  2:180889/251‑1 (MQ=255)                                                                                                                                                    gCACAAAATCTGAAAAACGTACCAGCATAAAAGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTTACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                    <  2:180796/251‑1 (MQ=255)                                                                                                                                                         aaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                    >  1:193642/1‑246 (MQ=255)                                                                                                                                                          aaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGGATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtct                                                              <  2:176688/251‑1 (MQ=255)                                                                                                                                                                          cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATc                                              >  1:513958/1‑251 (MQ=255)                                                                                                                                                                                aaaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTgg                                        >  1:19346/1‑251 (MQ=255)                                                                                                                                                                                    gATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCTGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGttt                                                                          >  2:426550/1‑213 (MQ=255)                                                                                                                                                                                    gATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGttt                                                                          <  1:426550/213‑1 (MQ=255)                                                                                                                                                                                                  aTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTg                      <  1:155360/251‑1 (MQ=255)                                                                                                                                                                                                            ttATCAGGATAGATACTTTCTTTAGGAATTTTTGTTTTATTACAGAACGTGCTGATACGTGTTGTAATTCTTCTCGGCTGACTCAGTCATGTCGTTTCTTAATGTTTTAGCCGGTTTTTTCTCCCGTCAATTCCTTGAATCAGCCTATTTAGCCCGTTTCTTCGACGTTTAATGCATTATCCCCTGTTTTTAGTGAGATCTCTCCCACTGTCGTATCATTTGTTCCGCCCGATACAGGTTGGccggcttgc            >  2:62616/2‑244 (MQ=255)                                                                                                                                                                                                              aTCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAGTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAAt          <  1:363467/251‑1 (MQ=255)                                                                                                                                                                                                                      aGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGc  >  1:99466/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          AAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGC  >  NC_000913/2101503‑2101966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.