Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	218.1 / NA	66	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (36/30);  total (36/30)

CTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCG  >  NC_000913/3560210‑3560688                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           cttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTAC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGAGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TAGAAGGCAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                        >  2:5412/1‑246 (MQ=255)   tttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                                                                                                                                                                      >  1:345872/1‑251 (MQ=255)            aaCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTg                                                                                                                                                                                                                            >  1:482975/1‑251 (MQ=255)                  ttAGCAACGTTGAGATTGTTGGTGACATTGCGCAAATTGCTGTAATTGAGCAGTGCGTGCGCTACCGCTCCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCg                                                                                                                                                                                                                       <  1:89202/250‑1 (MQ=255)                       aaCGTTGAGATTGTTGGTGCCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGACC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCCGGAA‑CAGGTTTTGGTCCGcccgcc                                                                                                                                                                                                                 >  2:105720/1‑247 (MQ=255)                       aaCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCATCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                 >  1:541821/1‑251 (MQ=255)                            tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                            >  1:221209/1‑251 (MQ=255)                                 ttgttgGTGACAACGCGCAAATTGCCGAGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCGTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCGATGATGGCGAAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                       >  1:169519/1‑251 (MQ=255)                                 ttgttgGAGACAATGCTCAAATTACTGTGATTGAGCAGTGAGTGCGCTACCGCTTACGGAGTGGTGCCGATATCGATAAACAGCGTCGCGCCATT‑GGGGGTTTGC‑TCCGCCACTTTG‑CGGGCGAGGCGCTCTTTGTCTTCGGGCCGGGTGGACTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAAGCAGCACCTCACCGCCATGATGGCGCAGGATACAG‑TTTTGCTCCGCCAGCTCAAAGAGGTc                                                                                                                                                                                                       >  2:484239/1‑251 (MQ=255)                                     tgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                                                                                                                   >  1:477268/1‑251 (MQ=255)                                      ggtaagaatgcgaaagtgccTGTGGTTGAGCAGTGCGTGCGTTTCCGTTTCCGGCGTGGTGCCTATATCGAAAACCCGCGTCGAGCCTTG‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGTGGCGCTCTTTTTCTTCGGTCTGGGTGGCTTGGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                                                                                                                                  <  1:135550/232‑1 (MQ=255)                                         gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACCGCGTCGAGCCATT‑GTGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGCAC‑TGTAAGGCAGCGCAGCACCGCCATGATGGCGCATGAT‑CACGTTTTGCTCCGCCAGCTCATAGCGGTCGCGGCGa                                                                                                                                                                                                >  2:434836/1‑250 (MQ=255)                                         gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCCGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                                >  1:333325/1‑250 (MQ=255)                                                               tggcggagtgcgggcccacccGCTCCCGGCGTGGTGCTAATATCGAAAAACAGCTCCGTGCCTTT‑GGGGATTTGC‑TCCGCC‑CTTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGTTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                                                                                                                          <  1:508648/231‑1 (MQ=255)                                                               gcgagcggggcgggcgatACCGCTTCCGGGGTGGTGCGGATATCGATAAACAGCTGGGAGCCGTT‑TGGGTTTTTC‑TCCGCCACTTTG‑CGGGCGAGGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGGTCTTGCCCCGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGGCAGCTCACTGATGTCGCGGCGAATAGTCTGCGGGCGGACGGAGaa                                                                                                                                                                         <  1:205683/234‑1 (MQ=255)                                                                  aGCAGTGCATGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGCGCCATT‑GGGGCTTTGC‑TCAGCCACTTTA‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGCAGGCCTTGCGATCGTGCCACTGCGTGTTAAC‑CGAAC‑TGGCAAGCGGAGACGCACAGCCATGCTCGCGCCGGAT‑CAGGTTTTGCTCCGCAAGCTCATTGAGGTCGCGGCGAATAGGCAGCGGGCTGACGGAGAAATg                                                                                                                                                                      >  2:494472/1‑251 (MQ=255)                                                                       tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCAATT‑GGGGATTTGC‑TCCGCCACTTAG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGCAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAATGGTCTGCGGGCTGACGGAGAACTGctct                                                                                                                                                                  >  1:530864/1‑250 (MQ=255)                                                                         cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGGTGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                               >  1:388922/1‑251 (MQ=255)                                                                               cTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                         >  1:307375/1‑251 (MQ=255)                                                                                tACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAGATGCTCTACCAGATCtt                                                                                                                                                        >  1:105074/1‑251 (MQ=255)                                                                                 aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCCGCAGGAGAACTGCTCTACCATCTCTTc                                                                                                                                                       >  2:484636/1‑251 (MQ=255)                                                                                  ccgctccgggCGTGGGGCCGATCGTGATAAACAGCGGCGA‑CCATTTGGGGGTTTGC‑CCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGTTCTGGGTGGCCTTGCGATCGTGCCTCGGCGTTTTAAC‑CGAAC‑TGTAAGTCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                                                                                                                      <  1:82402/243‑1 (MQ=255)                                                                                   cGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                                                                                                                     >  1:555056/1‑251 (MQ=255)                                                                                        ccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACtt                                                                                                                                                >  2:96783/1‑250 (MQ=255)                                                                                          ggCGTGGAGCCGATATCGATAAGCAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGAAGCGCCGCACCGCCATGATGGCGCAGGAG‑CAGGTTTTGCTCCGCACGCTCCTTGCGGTCACGGCGACTAGGCTGCGGGCTGACGGAGACATGACCTGCCAGCTCTTCAGTACTggc                                                                                                                                              >  2:467782/1‑249 (MQ=255)                                                                                            cGTGGTGCCGATATCGATAAACAGCGTCGAGACATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGGTGGCGCAGGAT‑CCGGTTTTGCTCCGCCAGCTCATTGAgg                                                                                                                                                                                                         >  2:554625/1‑190 (MQ=255)                                                                                               ggtgCCGATACCGATAACCAGCGTCGAGCCAT‑‑GGGGTTTTGCCTCCGCCCCTTTG‑GGGGCGATGCGCTCTTTTCCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCTTTTTAAC‑CGAAC‑TGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                         <  1:536511/251‑1 (MQ=255)                                                                                                  gCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                      >  2:570147/1‑251 (MQ=255)                                                                                                   ccGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCAGTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                                                                                                                     >  1:132466/1‑251 (MQ=255)                                                                                                         tcaaaaaaagggctgggcctagt‑GGGGGTTTGCCTCCGCCCCTTTG‑CGGGCGATGCGATTTTTTTCTTGGGTCGGGGTGGCCTTGCGATCGGGCCCGGGGGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                 <  2:80919/228‑1 (MQ=255)                                                                                                           gATAAACAGCGGCGGGCCATG‑GGGGATTTGC‑TCCGCCTCTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa                                                                                                                             <  1:450827/251‑1 (MQ=255)                                                                                                            aTAAACAGGGTCGACCTATG‑GGGGTTTTGC‑CCCGCCTCTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAAC‑CGAGC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                               <  1:509411/248‑1 (MQ=255)                                                                                                                  ggcggcGAGCCATT‑GGGGATTTGT‑CCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                      <  1:388831/246‑1 (MQ=255)                                                                                                                    cGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCCGTTCg                                                                                                                     >  2:132456/1‑250 (MQ=255)                                                                                                                     gTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat                                                                                                                   >  1:567255/1‑251 (MQ=255)                                                                                                                     cggcgtgcccttgtgttgttggccccGCCATTTTG‑TGGGCGGTGCGCTGTTTTTCTTCGGTCTGGGGGCCCTTGGGATCGTGCCACGGTGGGTCAAC‑CGAAC‑TGGAAGCCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                       <  2:477629/225‑1 (MQ=255)                                                                                                                              tt‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGATGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CCGGTTTTGCTCCGCCAGCTCTTTGCGGTCGCGGCGAATCATCTGTGGGCTGACGGAGAGATGCTCTACCAGCTCTTCGATATTGTCATTACCCTGCGGTTTTACAAGTTCGCTAATCCCGtt                                                                                                          >  2:252583/1‑251 (MQ=255)                                                                                                                              gt‑GGGGATTCTC‑TCCGCCACTTTG‑CGGGCGATGCTCTTTTTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                                           <  1:73010/249‑1 (MQ=255)                                                                                                                                   gtgttgtgtctccgcttcttccggggCGATGCGCTCTTTTTTGTGGGTCTGGGTGGCTCTGCTACCGGACTCCGGCGTGTTAAC‑CGAAC‑TGGAATTCAGCGCTGCTCCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                        <  2:171169/228‑1 (MQ=255)                                                                                                                                         tgccccGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACC‑TGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                  <  1:426700/246‑1 (MQ=255)                                                                                                                                                                  cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                         <  1:484636/251‑1 (MQ=255)                                                                                                                                                                  cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                         >  1:129710/1‑251 (MQ=255)                                                                                                                                                                   gcTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                        <  1:105720/251‑1 (MQ=255)                                                                                                                                                                      ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTgg                                                                       <  1:132456/249‑1 (MQ=255)                                                                                                                                                                       tttttcgtggggcTGGGTGGCTTTGCGATCGTGCACCGTCGTGTTAAC‑CGAAC‑GGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa                                                                     <  1:494472/238‑1 (MQ=255)                                                                                                                                                                       tttttCTGCGGTCGGGTTGGCCTTGCGATCGTGCCACGTCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCTCCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa                                                                     <  1:251073/250‑1 (MQ=255)                                                                                                                                                                        ttttCTTCGGGGTGGGGGGCCTTGCGAGGGTGCCGCGGGGT‑TTAACACGACG‑TGGAGGGCAGCGCAGCACCGACATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTTTGTTTGTTTCATTTATAAATCCCTGGaa                                                                     <  2:108094/249‑1 (MQ=255)                                                                                                                                                                            ccttggtgggggTGGCTTTGCGATCGTGCACCGGTG‑GTTAAA‑CCAACATGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTTTTTCATTTATAAATCCCTGGAATTAttt                                                               <  2:567834/242‑1 (MQ=255)                                                                                                                                                                              cgggccTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttc                                                             <  1:570147/246‑1 (MQ=255)                                                                                                                                                                                    tGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                       >  1:402688/1‑251 (MQ=255)                                                                                                                                                                                       gTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                    >  1:536450/1‑251 (MQ=255)                                                                                                                                                                                            cTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                               >  1:384707/1‑251 (MQ=255)                                                                                                                                                                                              tGCGATCGTGCCCCGGGGTGTTAAC‑CGAAC‑GGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATACCTGGAATTATTTTCGTTTTCGCGCATTGa                                              <  1:467782/250‑1 (MQ=255)                                                                                                                                                                                               gagATCGTGCCACGGCGAGTTAAC‑CGAAA‑TGGAAGGCAGAGCCGCACAGCCCTGATGGCGCAGGAT‑CAGGTTTTGCTACGCCAGCGCATTGAGGTCACAGCGAAAAGTCTTCGGGCTGACGGAAAAATGCTCTAACAGGTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGCTCGATAATACCGTTGCGACGATACGTTTGTTTCATTTATTGATCCCTGGAATTATTTTCGTTTTCGGTCATTGAgg                                            >  2:437838/3‑250 (MQ=255)                                                                                                                                                                                                     gTGCCACGGCGTGTTACC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca                                      <  1:341531/251‑1 (MQ=255)                                                                                                                                                                                                            ggCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                 >  1:136421/1‑249 (MQ=255)                                                                                                                                                                                                             gCGTGTTAAC‑CGAAC‑GGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc                              <  1:160582/251‑1 (MQ=255)                                                                                                                                                                                                                tgtTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                    <  1:510246/146‑1 (MQ=255)                                                                                                                                                                                                                tgtTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTACGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                    >  2:510246/1‑146 (MQ=255)                                                                                                                                                                                                                  ttacc‑cGAAC‑GGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                          <  1:106092/246‑1 (MQ=255)                                                                                                                                                                                                                         gAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc                   <  1:140885/251‑1 (MQ=255)                                                                                                                                                                                                                                  aGGCACCGCAGCACCGCCATGATGGCGAAGGAT‑CAGGATTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCAATCGCTAAAACCAAGGCt           >  1:157870/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGATTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                    >  1:467126/1‑177 (MQ=255)                                                                                                                                                                                                                                    gcctcgccgcATCGCCCTGATGGCGCTGGAT‑CAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAGTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTaa         <  2:541821/247‑1 (MQ=255)                                                                                                                                                                                                                                      aGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTACACCCACGGCTAAcc       >  2:457904/1‑251 (MQ=255)                                                                                                                                                                                                                                         gccgcACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg    >  1:298169/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggcACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg  <  1:294946/250‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           CTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATT‑GGGGATTTGC‑TCCGCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAC‑CGAAC‑TGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCG  >  NC_000913/3560210‑3560688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑