Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	226.0 / NA	52	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (27/25);  total (27/25)

CGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGT  >  NC_000913/3207303‑3207792                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     cgtcgCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTTCCCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGcccc                                                                                                                                                                                                                                                   <  1:299038/250‑1 (MQ=255)    cgcgGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGCCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACTTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATc                                                                                                                                                                                                                                               <  1:366260/250‑1 (MQ=255)       cgctcgcgtctgGTTCAGGCCGTTCTCACCTGT‑AAAAACTGGCTCCACCCCCGGAGCGCGTTAGAAAAAAGTCCCTGCCACCCCCGACCAAAGCTGAAGATCTGCAAGATATTCGCGTGGGCGATGGTATCTCCCGGCCCGTCACGTTGGTGACCTCCCGCGACGTTGG‑TCACCGCCGTTTGATCGAACTGAACCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgc                                                                                                                                                                                                                                           <  2:416624/242‑1 (MQ=255)           cGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCa                                                                                                                                                                                                                                        >  1:566441/1‑250 (MQ=255)                 tgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                                 >  1:443340/1‑251 (MQ=255)                 tgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACg                                                                                                                                                                                                                                  <  1:170284/250‑1 (MQ=255)                  ggTTCATGCCGATCTCACCTTTGCAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGACCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCACAACCGGc                                                                                                                                                                                                                                >  2:552726/1‑251 (MQ=255)                            taTCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATAAAAAAGATCCTGACAACACCGATCAAACCTGAAGATCTGAAAGATATTCGCGTCGGCGATGAGATCTACCTGACCGGTACGCTGGAGACATGCCGCGGCGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGGACGCTCCCTTAAGATCTCACCGGCAAAGCGATTTTCCACGCTG‑CCCAATCGTGGGCAACAACGGCGAAAAATggg                                                                                                                                                                                                                      >  2:336687/2‑251 (MQ=255)                                 ccccTTTTAAAATCTGTCCC‑ACACCCGGAACCCGTGATAAAAAAGTCCCTGACAACCCCTATAAATGCGGAATGTCTGCAAGATATGCGTGTCGCCGTTGTGTCCTCCCGGACCGGTGCGCTGGTCACCGCCCCCGACTTTTG‑TCTCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATg                                                                                                                                                                                                                 <  2:527148/249‑1 (MQ=255)                                                   tc‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTAGTGACCTGCCGCGACGTTTG‑TCACCGCCGCTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑TCCCATCGTGCGCAAAAACGGCGCCAAATGGGAAATGGTCTCCGGCGGCCCGAc                                                                                                                                                                                                >  1:272687/1‑250 (MQ=255)                                                    c‑acaCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGGGAGGGTCTCCGTCGGCCCgcac                                                                                                                                                                                               >  2:456138/1‑247 (MQ=255)                                                     cccccccGGAGCGCGTTAGGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGa                                                                                                                                                                                                 <  1:60765/244‑1 (MQ=255)                                                              atcgcgTTATGAAAAAGATCCGGACACCCCCGATCAAAGCTTAAGATCTGCAAGATATGCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGc                                                                                                                                                                                     <  1:230414/249‑1 (MQ=255)                                                                  gttgaaaaaaaaGACCCTGACACCCCCGATCAAAGCGTAGGACCTACAGAAAATTGTCGTCGGCGATGTGCTCTCCCTGCCGGGCACCCTGTTGACCTGCCGGGACGTTTG‑CCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCTACAAATGGGAGATGGTCTCCGTCGGCCCTACAACCAGTATGCGTAt                                                                                                                                                                                 <  2:73762/244‑1 (MQ=255)                                                                  cgTTATGAAAAACATCGTGACAACCCCGATCAAAGCTTAAGATCTGCAAGAGATCCGCGTCGGCGAGGTGACCTACCTGACCGGTTCTCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTAt                                                                                                                                                                                 <  1:131423/251‑1 (MQ=255)                                                                                          cccGATCAAAGCTGAAGATCTGCAAGATATTCGTGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGATTTGAACGTGAATTTAt                                                                                                                                                         >  1:343929/1‑251 (MQ=255)                                                                                             gATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTgg                                                                                                                                                      >  1:121164/1‑250 (MQ=255)                                                                                                          gATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                         >  1:115395/1‑251 (MQ=255)                                                                                                                cAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtg                                                                                                                                   >  1:234183/1‑251 (MQ=255)                                                                                                                    aatttCGCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggtgg                                                                                                                               <  1:378631/248‑1 (MQ=255)                                                                                                                     tatTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTggtggt                                                                                                                              >  1:154574/1‑251 (MQ=255)                                                                                                                      atTCGCGCCGGCGATGTGATCTCCCTGACCGGTCCGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGtt                                                                                                                             <  1:56417/251‑1 (MQ=255)                                                                                                                         cgcgTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGc                                                                                                                          >  1:398210/1‑251 (MQ=255)                                                                                                                           cgTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTACGCAGACCGGCGTGAAACTGGTGGTTGGCa                                                                                                                         >  2:525739/1‑250 (MQ=255)                                                                                                                               ggCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCTTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACACTG‑CCCCATCGTGCGCAACAACTGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGACAGTTTTGAACGTGAGTTTATTGAGTCGACCGGCGTGAAACTGGTGGTTGGCA‑AAggt                                                                                                                   >  2:177755/1‑251 (MQ=255)                                                                                                                                           tACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑ACGGTGGGGTGGGGCCg                                                                                                       >  2:67905/1‑251 (MQ=255)                                                                                                                                             ccggtgctggacgcggtgggccTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGTTGGCA‑AAGGTGGTATGGGGCCGCt                                                                                                     <  2:370891/231‑1 (MQ=255)                                                                                                                                              cggtccggtcagCTGGTGACCCGCCGCTA‑GTTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGAGCCCTTACGATCGCAACGGCAAAGCGATTTTCCGCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATTGGGCCGCTg                                                                                                    <  2:282435/240‑1 (MQ=255)                                                                                                                                               cgcccGGTCCGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGa                                                                                                   <  1:493408/248‑1 (MQ=255)                                                                                                                                                  ccGGTACGCTGGTGACCTGCCACGACGTTTG‑TCACCGCCGTTTGATCGACCTGAAACGTCCGATCCCTTACGATCTCAACGGCGAAGCGATTTTCCACGATG‑CCACATCGTGCGACAAACCGGCGACAAATGGGAGATGGTCTCCGGCGGACCGCCAACCAGTATGCGAATGGAAAGTTTTGAACGTGAATTTATTGAGCAGCCCGGCGTGAAACTGG‑GGGGGGAACAAGGGGGTATGGGGCCGCTGGCCg                                                                                                >  2:528053/1‑251 (MQ=255)                                                                                                                                                     gTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCt                                                                                                     >  1:370891/1‑243 (MQ=255)                                                                                                                                                            ggTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTg                                                                                      >  1:143123/1‑251 (MQ=255)                                                                                                                                                             gTGACATGCCGCGACGTTTG‑TCACCCCCGTTTGATCGAACTGAAACGTCAGACCCCTTACGATCTCAACGGCAACGCGATTTTCCACGCTG‑CCACATCGTGCGCAAAAACGGCAACAAATGGGACATGGTCTCCGTCGGCCCGACAACCCGTCTGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCCAACCGGCGGGAAACCGGTGGTTGGCA‑AAGGTGGTAAGGGGCCGCTGACCgacaaagacttc                                                                                     >  2:262849/1‑241 (MQ=255)                                                                                                                                                              tGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                    >  1:390683/1‑251 (MQ=255)                                                                                                                                                                     ccGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGTCAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGCCCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGCAGGCTGCCAGagatg                                                                             >  2:318919/1‑247 (MQ=255)                                                                                                                                                                     ccGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAg                                                                                  <  1:318919/246‑1 (MQ=255)                                                                                                                                                                         gACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCCACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGCCAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGAGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAAATGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCa                                                                           >  1:68891/1‑249 (MQ=255)                                                                                                                                                                         gACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAg                                                                         <  1:552726/251‑1 (MQ=255)                                                                                                                                                                                    cccgccgTGTGATCTAACTTAATCGTCCGATCCCATACCATCTCACCGTCAAAGCGATTTTCCTCGCTG‑CCCCATCGTGCGCAAAAACGGCGCAAAATGGGAGAGGGTCTCCGTCAGCCCGACATCCAGTATGCGGATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGGTGTATGGGGCCGGTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtg                                                               <  2:348553/250‑1 (MQ=255)                                                                                                                                                                                          gTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCAAGCTG‑CACCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAGGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACAGGCGTGAAACTGGAGGTTTGCA‑AAGGTGTTATGGGGCCGATGACAGCGGAAGGCTGCCAGAAATTCAAGGCGATCCATGTGAttt                                                         >  2:475673/1‑251 (MQ=255)                                                                                                                                                                                              gATCGAACTGAAAAGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCACGGCGCTACATGTGATTTTccc                                                     >  2:249128/1‑251 (MQ=255)                                                                                                                                                                                                        aaaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgc                                           <  1:545522/251‑1 (MQ=255)                                                                                                                                                                                                                gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCt                                   >  1:521695/1‑251 (MQ=255)                                                                                                                                                                                                                   cccTTACGATCGCAACGGCAAAGAGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggc                                <  1:14833/251‑1 (MQ=255)                                                                                                                                                                                                                        aCGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCa                            >  1:368856/1‑250 (MQ=255)                                                                                                                                                                                                                               tcaCGGCCAAGTGATTTTCCCCTCTG‑CCCCAACCTGCGCAAAAACTGCGACAAAAGGGGTGTGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAcc                         <  2:390683/244‑1 (MQ=255)                                                                                                                                                                                                                               cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAc                          <  1:311560/245‑1 (MQ=255)                                                                                                                                                                                                                                aaCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGAAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTg                   >  1:212651/1‑251 (MQ=255)                                                                                                                                                                                                                                 aCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTgg                  <  1:336687/251‑1 (MQ=255)                                                                                                                                                                                                                                    gCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAag               <  1:15345/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAAAGCGATTTTCCACGCTG‑CCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACATCCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAaga              <  2:266590/251‑1 (MQ=255)                                                                                                                                                                                                                                              gttCCACCCTG‑CCCCATCGTGCGAAAAAACTGCGACAAATGGGAGATGGGCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGTCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTACCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAATAGATTgaat      <  2:206307/249‑2 (MQ=255)                                                                                                                                                                                                                                                 ccACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGt  <  1:482909/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     CGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTC‑ACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTG‑TCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCA‑AAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGT  >  NC_000913/3207303‑3207792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑