Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,299	2 bp→GA	intergenic (+5/‑15)	radA → / → nadR	DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,299	0	A	G	95.9%	153.5 / ‑2.4	50	intergenic (+5/‑16)	radA/nadR	DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (17/30);  total (18/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01
*	NC_000913	4,627,300	0	T	A	92.3%	154.1 / 2.0	52	intergenic (+6/‑15)	radA/nadR	DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (17/31);  total (19/33)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCA  >  NC_000913/4627058‑4627539                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                 tCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACgga                                                                                                                                                                                                                                           <  1:525456/250‑1 (MQ=255)              aCAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGGTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATgtcgt                                                                                                                                                                                                                             <  2:166709/251‑1 (MQ=255)                          cacAGGATCTGGTGGTGTTTGGAGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGGCTTATAATGAGGAATACGGGGGGAGATATGTCGTCTTTTGATTAcc                                                                                                                                                                                                                 >  2:108868/3‑251 (MQ=255)                                    ggtggtGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAAc                                                                                                                                                                                                          <  1:564928/248‑1 (MQ=255)                                     gtggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGcc                                                                                                                                                                                                      <  1:123332/251‑1 (MQ=255)                                          gtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa                                                                                                                                                                                                  <  1:33711/250‑1 (MQ=255)                                          gtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa                                                                                                                                                                                                  >  1:673933/1‑250 (MQ=255)                                                       gggCTGGCAGTGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGc                                                                                                                                                                                    >  1:541912/1‑251 (MQ=255)                                                              cAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAc                                                                                                                                                                             >  1:415020/1‑251 (MQ=255)                                                                 gggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAcagc                                                                                                                                                                          >  1:622946/1‑251 (MQ=255)                                                                                     aGCGGTCAGGAACGAATGTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑Gt                                                                                                                                                     <  1:499498/251‑1 (MQ=255)                                                                                        ggTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACa                                                                                                                                                                                           >  1:34286/1‑211 (MQ=255)                                                                                        ggTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACa                                                                                                                                                                                           <  2:34286/211‑1 (MQ=255)                                                                                         gTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATg                                                                                                                                                  >  1:227851/1‑250 (MQ=255)                                                                                             ggAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCaa                                                                                                                                             <  1:465289/251‑1 (MQ=255)                                                                                                  gaattccagaattggcgAAACACGGTTTGCGTCGGGCGATTGGGCCGGCGGCGAAGGTACCGAAAAAAGCGCCGGAAAGTATTCAGAGTTTTGGCTTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCTACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCTCGCTACAGCAGGTGGCTGATGCCAGCG‑TTATGACCAAAGGGt                                                                                                                                        <  2:614445/238‑1 (MQ=255)                                                                                                      ttcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTAtt                                                                                                                                     <  2:636584/246‑1 (MQ=255)                                                                                                       tcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTAttt                                                                                                                                    <  1:516594/247‑1 (MQ=255)                                                                                                        cagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTaa                                                                                                                                  <  1:232563/249‑1 (MQ=255)                                                                                                           aaGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGcc                                                                                                                               <  1:3872/251‑1 (MQ=255)                                                                                                                gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCAGGACGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGtt                                                                                                                           >  1:92382/1‑250 (MQ=255)                                                                                                                 cgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCTCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGTGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCGATAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTAc                                                                                                                         <  1:435742/251‑1 (MQ=255)                                                                                                                      cacGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGCCGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGt                                                                                                                                                                      >  2:296111/1‑202 (MQ=255)                                                                                                                      cacGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGt                                                                                                                                                                      <  1:296111/202‑1 (MQ=255)                                                                                                                      cacGGTGTTCGCCGGGCGATTGTTCCGGCTGCTAACGTACCGAAAATAGCGCCTGAAGTGATGCAGATTTTTGTCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGTAATACGGAGGGTGATATGTCGTCATTTGATTACCTGAAAACTGCCATCATGCATCATGGCGGCACGCTATAGCGTGTTGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAAt                                                                                                                    <  1:635613/251‑1 (MQ=255)                                                                                                                       acGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATg                                                                                                                   <  1:313528/251‑1 (MQ=255)                                                                                                                               cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGCCTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCAAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATACCAAAATc                                                                                                           >  1:364779/1‑251 (MQ=255)                                                                                                                               cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt                                                                                                            <  1:675795/250‑1 (MQ=255)                                                                                                                                gcagggCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCTGAAGTGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTAGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa                                                                                                          <  1:357393/248‑1 (MQ=255)                                                                                                                                   gggCGATTGTGCACGCGGCTAACGTACAGAAAAAAGCGCCGGAAGGGATGGAGGTTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCTACGACTTATAATGAGGAATCCGGAGGGAGATATGGCGTCATTTGATTACCTGAAAACTGCCATCAAGCCACAGGGCTGCACTCTACAGCAGGTGACTACTGCCA‑CGTGGATGACCCAAAGGTATTTACGCCAGTTACTGACTGCCCAAATccaa                                                                                                        >  2:89061/1‑247 (MQ=255)                                                                                                                                    ggCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAg                                                                                                      <  1:446311/251‑1 (MQ=255)                                                                                                                                        aTTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                                                                                                  >  1:593209/1‑251 (MQ=255)                                                                                                                                          tGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                                                                                                  >  2:36288/1‑249 (MQ=255)                                                                                                                                             tCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATCTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg                                                                                              <  2:593209/250‑1 (MQ=255)                                                                                                                                                       aaCGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg                                                                                   <  1:31547/251‑1 (MQ=255)                                                                                                                                                                aaaaaaGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTg                                                                          <  1:4445/251‑1 (MQ=255)                                                                                                                                                                       cgcCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGtt                                                                   >  1:99347/1‑251 (MQ=255)                                                                                                                                                                        gcCGGAAGGGAGGCAGATTTTTGGCGGTATAAAACTCTCCGACGCGCTTAGCGTGGTCGTCGACTTATAATGAGGAATACGGAGGTAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGTTGCACGCTACAGCAGGTGTCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTTGAGTCGTTGCACCGttt                                                                  <  2:521929/251‑1 (MQ=255)                                                                                                                                                                          cGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGttttt                                                                <  1:454887/251‑1 (MQ=255)                                                                                                                                                                             aaGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTgg                                                             <  1:36288/251‑1 (MQ=255)                                                                                                                                                                                  aTGCAGATTTTTGGCGATAAAACACTCTCCGACGCGCATAGCGTGTTCGACGACTTATAATGAGGAAAACGGAGGGAGATATGTCGTCCTTTGATTACATGAAAACTGCCGTCAAGCAACAGGGCTGCACGCTACAGCAGGTGGATGCTACCCGCG‑GTCTGACCACAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCCGCGCGCAAAAGCTGGAGGCGGTGCACCGTTTTTTGAGGCtt                                                        >  2:650494/1‑251 (MQ=255)                                                                                                                                                                                             gtgCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTcccc                                              <  2:576170/248‑1 (MQ=255)                                                                                                                                                                                              ggCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCg                                             <  1:391308/250‑1 (MQ=255)                                                                                                                                                                                                          ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc                                                                                     <  2:215848/198‑1 (MQ=255)                                                                                                                                                                                                          ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc                                                                                     >  1:215848/1‑198 (MQ=255)                                                                                                                                                                                                                gACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAAccattggt                          <  1:108868/251‑8 (MQ=255)                                                                                                                                                                                                                      cTTAGCATGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTACACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAACGGGTATTTAAGCCAGTTACTGACTGCCAAAATCAAAAGCCCCAGCGCGCAAACGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTACCAGGAAGAAGAAAACAATTGgtgtggtt                    >  2:572529/1‑247 (MQ=255)                                                                                                                                                                                                                            gtgtTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGACTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGATGGCGGCGTTGCCCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGt              >  2:676740/1‑251 (MQ=255)                                                                                                                                                                                                                                         tataATGAGGAATACGGAGGGAGGTATGTCGTCATTTGATTACCTGAAAACGGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGTCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCTCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAATTTCTcccc   <  2:149109/249‑1 (MQ=255)                                                                                                                                                                                                                                         tataATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcccg  <  1:676740/250‑2 (MQ=255)                                                                                                                                                                                                                                                 gaTATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTTCACGCTACAGCAGGTAGCTTATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGa                                                      <  2:1328/190‑1 (MQ=255)                                                                                                                                                                                                                                                 gaTATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGa                                                      >  1:1328/1‑190 (MQ=255)                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                 TCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCA  >  NC_000913/4627058‑4627539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑