Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,729,4400CT95.7% 135.3 / ‑3.8 47noncoding (106/160 nt)xylZsmall RNA XylZ
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (24/20);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,729,4410CT95.7% 140.3 / ‑2.9 47noncoding (105/160 nt)xylZsmall RNA XylZ
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (24/21);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGC  >  NC_000913/3729210‑3729670
                                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                     
gcgATCAGTTGCCTGCCCCCACTGTCCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATa                                                                                                                                                                                                                    <  2:649932/251‑1 (MQ=255)
gcgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGGCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAACATGCCCGGTATCGCTACCGATAACCGCGCCACCGGACTGCACAGTTAGCCGTTATTTGTCGAACAgct                                                                                                                                                                                                                     >  1:106657/1‑248 (MQ=255)
           cctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCa                                                                                                                                                                                                         <  1:218954/251‑1 (MQ=255)
              gccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTAcc                                                                                                                                                                                                          >  1:229919/1‑247 (MQ=255)
               ccaccaCCGTTCCGGGTATTGTTCCGACCAGCGCGGATGCGGACGCGAAACGGTAAGCTTTTCAGCTTACGCAGCAACCACCTCACCCTGCACGTTGAGCAAAATAACTTTTACGCCCGAGGAGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCCACCGATAACCGGGCAACCGGACTACCCAGTTAGTTGTTATTTTTTGACCAGATAATGGTTTACCAGaat                                                                                                                                                                                                     >  2:282563/1‑249 (MQ=255)
                    ccggTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCa                                                                                                                                                                                                <  1:407802/248‑1 (MQ=255)
                       gTTCCGGGTCTTGTTCCGACCAGAGTGGAGGCGGGCGAGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGTTCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGTTAACCGGGCCAACGATCAGCACCGGTAGTTGTTATTTGTAGAAAACATAATGGTTTACCAGTTTTTCCTgtt                                                                                                                                                                                             >  1:12255/1‑251 (MQ=255)
                           cGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGATCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTc                                                                                                                                                                                         >  1:115972/1‑251 (MQ=255)
                               tCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTAAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACCTATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCACCGGCCTGCACAGTTAGTTGTTTTTTGTCGAACAGATCATGGTTTCCCAGATTTTCAAGTTTTTCCTgg                                                                                                                                                                                     >  2:199071/1‑251 (MQ=255)
                                          ccagaggtgagtcGGGCTCGGAACGGTCAGCTTTTCCGTTTGCGCCGCATCCACCGCACCCTGTTCGATGAGCAAAAGAACTTGTTCGCCCGAGGTGCCAAGATCTAGCCCGATATACACATCGATCGTTTCTTAAAAAAAGTCCCGCTAGCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTACTTGTCGACCAGATAATGGTTCACCGGATTTTCCATTGGTTCCTCGCTACCACTCTg                                                                                                                                                                          <  2:664347/239‑1 (MQ=255)
                                            agagTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGAt                                                                                                                                                                        >  1:198068/1‑251 (MQ=255)
                                                 ggATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGGTCCTTAAAAAAATGCCCGGTATCGCAACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATACTGGTTTACAAGATTTTCCAGTTGTTCCTGGCGAACACTCTGATGCAcc                                                                                                                                                                   >  2:314316/1‑251 (MQ=255)
                                                      cGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCCCCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGAACGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgaga                                                                                                                                                              <  1:518084/251‑1 (MQ=255)
                                                      cGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCgg                                                                                                                                                                 >  1:410584/1‑248 (MQ=255)
                                                       gggCGCGAAACGGGCAAATTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATTTCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGTGCCAACGGATTTCACAGTTAGGTGTTAGTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTGGGTCCTGGCGACCTCTCTGATGCACCGgagaa                                                                                                                                                             <  2:119870/251‑2 (MQ=255)
                                                        ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTc                                                                                                                                                                                         >  1:601050/1‑222 (MQ=255)
                                                              aaaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatgat                                                                                                                                                      <  1:509454/251‑1 (MQ=255)
                                                                aCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGt                                                                                                                                                    >  1:205785/1‑251 (MQ=255)
                                                                  ggTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTc                                                                                                                                                  <  1:396375/251‑1 (MQ=255)
                                                                     cAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATATCTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTTCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCt                                                                                                                                                <  2:563519/250‑1 (MQ=255)
                                                                     cAGCTTTTCCGGTTGGGCAGCAACCACCTCACCCCGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGGTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTg                                                                                                                                               <  2:417568/251‑1 (MQ=255)
                                                                           ttCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCat                                                                                                                                          <  1:366498/250‑1 (MQ=255)
                                                                             ccGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATAtt                                                                                                                                       <  1:22498/251‑1 (MQ=255)
                                                                                   gcgcAGCAACCCACTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCt                                                                                                                                  <  1:214565/250‑1 (MQ=255)
                                                                                          aaCCACCTCACCCTGATCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGAAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGc                                                                                                                          >  2:676587/1‑251 (MQ=255)
                                                                                               ccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGCTCGTTCCTTAAAACAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTg                                                                                                                     >  1:436546/1‑251 (MQ=255)
                                                                                                            gTTGAGCAAGATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGATACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCttt                                                                                                        <  1:202115/251‑1 (MQ=255)
                                                                                                               gAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAg                                                                                                     <  2:229919/251‑1 (MQ=255)
                                                                                                                          cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGc                                                                                          >  1:241945/1‑251 (MQ=255)
                                                                                                                           ttttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCCACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGcc                                                                                         >  2:187064/1‑251 (MQ=255)
                                                                                                                                    cGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCt                                                                                >  1:396826/1‑251 (MQ=255)
                                                                                                                                        gTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGAATATTTGACTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTc                                                                            >  1:553212/1‑251 (MQ=255)
                                                                                                                                            cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCACCGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATCATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTAAGCCTATTTGGCTAAATCTGCCAGTGCCCTTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGc                                                                        >  2:559904/1‑251 (MQ=255)
                                                                                                                                            cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCAAAGATAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGCGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGc                                                                        >  2:102343/1‑251 (MQ=255)
                                                                                                                                                 tCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATACCCGGTATCGCTACCGATAACCGGTCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGCTGCACCGGCGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGATGGCCCAATTCGCTTTTCCAGCCGGa                                                                    >  2:450700/1‑250 (MQ=255)
                                                                                                                                                    aTCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATaa                                                                <  1:600505/251‑1 (MQ=255)
                                                                                                                                                             taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGcgcg                                                       >  1:666197/1‑251 (MQ=255)
                                                                                                                                                                ataccgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAATTCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATg                                                    <  2:258738/247‑1 (MQ=255)
                                                                                                                                                                 tatCGATAGGTCCTTAAAAAAATGTCCGGTATCGCTACCGATAACCGGGCCAACGGAATGAACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACAAATCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGATAAATCCGCCAGTGACATTTGGCCATTCGGGATTTGATGGCCCAATTCGCTAATCCAGCCGGAATACAGCCGCGCGATgg                                                   >  2:460802/1‑250 (MQ=255)
                                                                                                                                                                     gatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGttt                                               >  2:223287/1‑251 (MQ=255)
                                                                                                                                                                        cgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTAt                                             <  1:270369/250‑1 (MQ=255)
                                                                                                                                                                           tCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGTCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCa                                          <  2:227610/250‑1 (MQ=255)
                                                                                                                                                                               tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACTCTGCGCGATGCGTTTATCCAGCTc                                      <  1:460802/250‑1 (MQ=255)
                                                                                                                                                                                          ccGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAAt                          <  1:193783/251‑1 (MQ=255)
                                                                                                                                                                                          ccGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAAt                          <  1:102343/251‑1 (MQ=255)
                                                                                                                                                                                              tATCGCTACCGATAACCGGGCCAACGGACTGCCCAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGTTGTTCCTGAGCATATTTGGCTAAATATGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGACCAATTCGCTATTCCAGCCGGAATACCGCGGCGCGATGCGTTTATACAGCGCGCCATCTTCatgcat                       >  2:478062/1‑245 (MQ=255)
                                                                                                                                                                                                                  ccAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAgcgc  >  1:646746/1‑251 (MQ=255)
                                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                     
GCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGC  >  NC_000913/3729210‑3729670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑