Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,497,3850TC91.7% 65.0 / ‑5.0 25F177S (TTT→TCT) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (12/10);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009131,497,3860T.96.0% 95.9 / ‑2.9 25coding (531/1656 nt)opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (12/12);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01
*NC_0009131,497,3870CA96.0% 67.4 / ‑4.6 25L178N (CTC→AAC) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (12/12);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01
*NC_0009131,497,3880TA96.0% 63.2 / ‑3.5 25L178N (CTC→AAC) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (12/12);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

GACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTG  >  NC_000913/1497159‑1497620
                                                                                                                                                                                                                                  |||                                                                                                                                                                                                                                          
gACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGcc                                                                                                                                                                                                                     <  1:506776/251‑1 (MQ=255)
                                ccGTCGACGCGTTCGGCTGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATAACACGATGCAGTTGTTGATACAAAACCATTAGAAGGGCAACGCGATATCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGACCTGGCGCGCCGTGATGACGTATCATC‑AACGGCGCGAGTTATTACCGCGCCGTGGCTGATCAATATCCATCCGGTTTGTCgat                                                                                                                                                                                     >  2:315913/1‑249 (MQ=255)
                                                aTGTTTTCTGTAGATCCAGCAACACTTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATc                                                                                                                                                                     <  2:475637/251‑1 (MQ=255)
                                                   ttttCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTAACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGACGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACAGCGCGAGATATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCgcc                                                                                                                                                                  >  2:188596/1‑249 (MQ=255)
                                                         gTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTa                                                                                                                                                             <  1:345619/250‑1 (MQ=255)
                                                                    aaCACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAAAAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGAGCGCCGTGATGTAGTATCATC‑AACGCCGCGAGTTATTTCCGCGCCGTTGATGATCCATATCAATACGGTTTGTAGGCACTCCACCTGGCGGTCGACACTTCCCCCGACAGTa                                                                                                                                                  >  2:664554/1‑250 (MQ=255)
                                                                                gcgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTccc                                                                                                                                     <  1:542467/251‑1 (MQ=255)
                                                                                 cgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTcccc                                                                                                                                    <  1:509155/251‑1 (MQ=255)
                                                                                                 gCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCt                                                                                                                    >  1:28623/1‑251 (MQ=255)
                                                                                                        gttgttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTg                                                                                                                                                                                          <  1:658528/174‑1 (MQ=255)
                                                                                                        gttgttCAAATACAACGATGAAGGTGTTGATACAAAACAATTAGAAGGGCAAAACGCTCTCGGCTTTGACGGTTTTCGCGTGTTTAAAGACCCCGAACTGGCGCGCCGTGAAGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTg                                                                                                                                                                                          >  2:658528/1‑174 (MQ=255)
                                                                                                                      aCGATGCCGGTGTTGATACAAAACCATTAGACGTGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAATCCCCCGACCTGGCGCGCCTTGATGTAGTATCATC‑AACTGCGCGCGTTATTTCCGCGCCGTTGATGATACATATCCATACGGTTTGTCGGCCCGCCGCCTGGCGATCTACACTTCCACCGACAATAAAGAAGAGTTCCCCGACTTTTCCTCCTTCTTGTTTTGAT‑CTGTAAAACCgg                                                                                              >  2:24791/1‑251 (MQ=255)
                                                                                                                                       aCAAAACTATTTGAGGGGAAAAGCGAGTTCTGCGTTGCCTTTTTTCACGTGTTTAAACCCCCATCACTGGTGCCCTGTGATGTAGTACCATA‑AACGGCTCGAGTTATTACCGCTCTGTTGATTATCCATATCAATACGGGTTGTGTGCCCGCGGCCTGTCTATCGTCACTTCCACCGACAGGAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGTTAAAACCGGGGGCaaattcttttac                                                                              <  2:35100/250‑11 (MQ=255)
                                                                                                                                        taaaaCAATTAGAAGAGCAAAGCGATCTCGGATTTGACGGTTTTCGCGAGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTAATTCCGCGCCGTTGCTGAACCATATCAATACGGTTTGTCGGCACGCGCACTGGCGATCGACACTTACACCGACAGTAAAGAAGCGTTCACCGACTTTACCGCCTTCT‑GGTTTGCTACGATAAACACAGGGCCAACTACCTTTACCg                                                                            >  2:352174/2‑251 (MQ=255)
                                                                                                                                           aaCAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGttt                                                                         >  1:238609/1‑251 (MQ=255)
                                                                                                                                               aTTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACGGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCACGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGCAACTTAAACCGACAGAAAAGAATAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACAGGGGGCAACTACCTTTACCGTTTCTGc                                                                     >  2:455414/1‑251 (MQ=255)
                                                                                                                                                                    cGGCTTGGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCa                                                    <  1:587501/247‑1 (MQ=255)
                                                                                                                                                                         ttaccGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGCTCGACACTTACACCGTCAGTAACGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGATCGATAGCGCCCGCATTACTg                                           >  2:220965/4‑251 (MQ=255)
                                                                                                                                                                                  gtCGCGTGGTTAAAGCCCCCGACCGGGCGCGCCGTGATGTAGTATCAAC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCTTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCtat                                   <  1:630549/249‑1 (MQ=255)
                                                                                                                                                                                    cgcgGGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAg                                <  1:44746/251‑1 (MQ=255)
                                                                                                                                                                                        tgtTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGAGAGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTACAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTc                             >  1:329866/1‑251 (MQ=255)
                                                                                                                                                                                            aaacgCCCCAGAACTGGGGCGCCGTGATGTAGTACCAGT‑AACGGCGCGCTTTATTTCCGTGCCGTTGAGGATAAATATCAATACGGGTGTTCGGCCCGCGTCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTAGAAGTTCACTAt                        <  2:294175/247‑1 (MQ=255)
                                                                                                                                                                                               aGCCCCCGAACTGGCGCGCCGTGATGTAGTAACATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCa                     <  1:673843/251‑1 (MQ=255)
                                                                                                                                                                                                      gcaCTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATtgtg                >  1:564893/3‑249 (MQ=255)
                                                                                                                                                                                                                  cGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTg  >  1:23553/1‑251 (MQ=255)
                                                                                                                                                                                                                                  |||                                                                                                                                                                                                                                          
GACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCT‑GGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTG  >  NC_000913/1497159‑1497620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑