| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,331,059 | 2 bp→AT | coding (12‑13/2598 nt) | topA → | DNA topoisomerase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,331,059 | 0 | T | A | 98.3% | 188.7 / ‑6.5 | 60 | A4A (GCT→GCA) | topA | DNA topoisomerase 1 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (33/25); minor base G (1/0); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,331,060 | 0 | C | T | 98.3% | 189.7 / ‑6.8 | 60 | L5F (CTT→TTT) | topA | DNA topoisomerase 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (35/24); minor base A (0/1); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CGCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGT > NC_000913/1330817‑1331301 || cGCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTTTCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCAt < 2:293393/251‑1 (MQ=255) gCTTTCGTCAAACTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACATGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGCAGGTTTATCAACGTGCGACGCATTCCTGGTAGAATCAAATTAGGAATGGGGAATATGGGTAAAGCATTTGTCATc > 2:499031/1‑251 (MQ=255) tCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGtt < 1:674093/250‑1 (MQ=255) cGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTg > 1:182434/1‑250 (MQ=255) aaTCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTTAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTcc < 2:55642/251‑1 (MQ=255) aTTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGACAGTGGAAGGTTTATCAACGTGCAACGCATTCCTGGAAGAATCAAATTAGGTAAGGGGCATATGGGTAAAGCCTTTGTCATCGTTGAGTCCCCGGCAAAAGcc > 2:606770/1‑250 (MQ=255) cTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑Cg > 1:3263/1‑251 (MQ=255) tGACATTCGACCAAAATTCCGTCGTAATATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACATGAATTTTCGGGAACAGAGTCACGACAAGGGGTTGATATCCGAAGAGAGCGAGTCCATATCGGTAAATCGATGCCAGTGGAAGGTTTATAAACATGCGACGCATTCCTGGATGCATCACATTAGGTAAGGTGAATGTGGGTAAAGCATTTATCATCGTTTAGTCCCAGGCAAAAGCCAACA‑CGAt > 2:325930/1‑251 (MQ=255) cATTCGACCAAAATGCCGTCGTGCTATTGCGCCTGTAGTCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGAGATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATcaa < 2:666010/251‑1 (MQ=255) ccAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGGGTCCCCGGCAAAAGCCAAAA‑AGATCAACAAGTAt > 2:579009/1‑251 (MQ=255) aaaaTTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCt < 2:3263/251‑1 (MQ=255) cgtGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGAc < 1:606770/251‑1 (MQ=255) cgtGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGAc < 2:138307/251‑1 (MQ=255) gCCAAGACCTGTTAACTCAGTAACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTTTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCg < 1:382991/251‑1 (MQ=255) ttAACTCAGTCAACTGAATTTTCGTGAACAGAGTCACGACAATGGGTTGATAGCCGCAGCGAGCGAGTCCATATCGGTAACTCGATGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGACGCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGTGTCCCCGGCAACAGCAAAAA‑CGATCAACACGTATCTGGGTAGTGGCTACGCTGTGAAATCCAGCGTCGGTCACATc > 2:402786/1‑251 (MQ=255) ccTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCg > 1:630574/1‑251 (MQ=255) tGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAg < 1:678790/162‑1 (MQ=255) aTTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAg < 1:85461/251‑1 (MQ=255) tGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAACTTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTc > 1:399058/1‑250 (MQ=255) cAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTg < 1:310553/251‑1 (MQ=255) agagTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGc > 1:646131/1‑251 (MQ=255) gACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGGTTATCCACGTGCGACGCATTCCTGGCAGCATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCCAAAGCCAAAA‑CGATCAACAAGTATCTGTGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACCTCCGCGATTGTCAGCCCCGTGGCTCAGCTGCCCAAAagt > 2:289447/1‑250 (MQ=255) aCAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTACCGACCAGTGGCTCAGCTGCCAAAAag > 1:438408/1‑249 (MQ=255) aaGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGCCGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg > 1:415885/1‑251 (MQ=255) aGGGGATGATATCCGCAGAGAGCGCGTCCATATAGGTAACTCGTTGCCAGTGGAAGGATTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGGAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCACCGGCAACAGCCAAAA‑CGATCAAAAAGTATCTGGGTAGTGACTACGTAGTGAAATCCAGCGTCGGTCACATCCGCGATTTGACGACCAGCGGCTCCGCTGCCAAAAAGAGTg > 2:240421/1‑250 (MQ=255) ggggTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑cc < 1:533860/251‑1 (MQ=255) tGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGAct < 2:296809/250‑1 (MQ=255) tCCGCAGAGAGCGAGTCCATATCGGTAACTCGCTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTACTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGAAAAAGCCAAAA‑AGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCACCTGCCAAAAAGAGTG‑CCAACTCTAccc > 2:73687/1‑250 (MQ=255) ccGCAGAGAGCGAGTCCATATCGGGAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAACTTCGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCCCCTCCGCGATTTGCCGACCAGTGGCTCAGCTGCCCAAAAG‑GTGGCCGCCTCTACCTc > 2:61523/1‑251 (MQ=255) gaagagagCGAGTACATATAGGAAACTCGTTGCAAATGGAAGGATTATAAACGGGAGAAGCAGTCATGGAAGAATCAAATTAGGTAATGTGAAAATGGGTAAAGCATTTGTCATCGGTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGAAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGAGTGTCCGACCAGTGGCTCAGATGCCAAAAAGAGTG‑ACGACTCTACCTcca < 2:607461/249‑1 (MQ=255) agagagCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTccacc > 1:455202/1‑251 (MQ=255) agagCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCGCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCaa < 1:543268/251‑1 (MQ=255) gagCGAGTCCATATCGGAAACTCGTTGCAAGTGGAAGGATTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTAACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAAGG‑CCGACTCTGCCTccacca > 1:271983/1‑249 (MQ=255) gCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGAc < 1:41755/251‑1 (MQ=255) gAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACgg < 1:579009/251‑1 (MQ=255) gAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACgg > 1:15791/1‑251 (MQ=255) gAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGGGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCAGGCAAACGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTAACATCCGCGATTTGCCGACCAGTGGCTCAACTGCCAAAAAGAGTG‑CCGACTCTACCTCCCCCAAGACgg > 2:557670/1‑251 (MQ=255) tCCATATCGATACCTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCCTTCCTGTAAGAATCACATTCGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTTAGTCCCCGGCAAAAGCCAAAA‑CGATCACCAAGTATCTGGGTAGTGACTACGTGGTGCAATCCAGCGTCGAGCACATCCGCCATTTGCCGACCAGTGGCTCAGCTACCACACAGAGTG‑CCACCACTACCTCCACCAACACgcct > 2:444877/1‑247 (MQ=255) ccATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTa < 1:54617/250‑1 (MQ=255) atCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCCAGACGGCTaaaa > 2:199776/1‑249 (MQ=255) aCTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTaaa > 1:238887/1‑250 (MQ=255) ttGCCAGTGGAAGGTTTATCAACGTGCTACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGAt > 1:326039/1‑251 (MQ=255) ggAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTgg > 1:471654/1‑251 (MQ=255) ggAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCAATTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTg < 1:444877/250‑1 (MQ=255) gAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGAc > 1:514542/1‑128 (MQ=255) gAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGAc < 2:514542/128‑1 (MQ=255) cAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTACGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCCAAAGCCAAAA‑CGATCAACAAGTATCTGGGGAGTGACTACGTGGTGACATCCAGCGTCGGTCACACCCGCGCTTTGCCGACCAGTAGCTCATCTGCCAAAACGCTGG‑CCGACTCTACCGCCACCAAGACGGCTAAAAAGCCTAAAAAGGAAGAACGCAGCGCTCTCGTc > 2:539574/1‑250 (MQ=255) aaCGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCa > 1:634692/1‑250 (MQ=255) cGTGAGACGCATTACTGGAAGAATCAACTTAGGTAAGGGGAATATGGGTAAAGCATTTGTCAGCGTTGAGTCCCCGGCAAAAGACAAAA‑CGATCAACAAGGATCTGGGTAGTGACTACGGGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAcc < 2:471654/251‑1 (MQ=255) aCGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATgg < 1:234436/251‑1 (MQ=255) cGCATTCCTTGATGAATCATATAAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAATGCCAAAA‑CGATCAACAAGTACCTGGGTAGTGACTACGAGGTGAAATCAAGCGTCGGTCACATCCGCGCTTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑ACGACTCTACCTCCACCAAGACGGACAAAAAGCCTAAAAAGGAGGAAcactgtcccctcgtaaaccggacggg > 2:576488/1‑226 (MQ=255) gCATTTCTGGAAGAATCAAATTAAGGAAGGGGGAAATGGGTAAAGCATTTGTCAACCTTTGGTCCCCCGCAAAAGCCAAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATggg < 1:234437/251‑1 (MQ=255) ccTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGATAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTc > 1:373707/1‑234 (MQ=255) aTTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAAtt > 1:442975/1‑251 (MQ=255) tggTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGCCCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGAGGCCAAATTggg > 2:88950/2‑251 (MQ=255) gggaaGGGGAATATGTGTAAAGCATTTATCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGTTTGACTCGTGGCACAATTGGGa < 2:455202/248‑1 (MQ=255) tGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCCCAATTGGGAGGCGCAc > 1:321552/1‑251 (MQ=255) ggAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCAc < 2:112604/250‑1 (MQ=255) ggTAAAGCGTTTGTCATCGTTGAGTCCCAGGCAAATGCCTAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCATCGTCGGGCACATCCGCGCTTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑ACGACTCTACCTCCACCAAGACGGCTAAATAGCCTGAAAAGGATGAACGTGGCGCTCTCGTCACCCGTCTGGGGGATGACCCGTGGCACAATTGGGGGGCGCACTATGa > 2:57837/1‑248 (MQ=255) ggTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAgg > 1:470001/1‑250 (MQ=255) || CGCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAA‑CGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTG‑CCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGT > NC_000913/1330817‑1331301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |