Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,512	G→A	E445Q (GAG→CAA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,512	0	G	A	100.0%	124.9 / NA	43	E445Q (GAG→CAA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)

ATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCA  >  NC_000913/4639273‑4639753                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  atttatttGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCt                                                                                                                                                                                                                                         <  1:236991/250‑1 (MQ=255)        ttcaggcgttATTTAAAGGCTGGCGCAACCGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGTTGGTGTGATGTGGGGACTTCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTTGTGCTGGCCGGCAAGATGTTTTTGACTCGTAATATCGACTGGTATGCGTTTTCTCTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGAGCAATTACGTATCTGGAAATa                                                                                                                                                                                                                                  <  2:528808/241‑1 (MQ=255)           aggCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGt                                                                                                                                                                                                                              <  1:291061/249‑1 (MQ=255)            ggctgTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCGGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGAGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGtt                                                                                                                                                                                                                             <  2:87648/247‑1 (MQ=255)                 tATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAAAGGCCGATCAATTACGTATCTGGAAATAAGGTTGaaa                                                                                                                                                                                                                         >  2:507914/1‑250 (MQ=255)                     gAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCTAAAATGAAAGCCAGTAAAGAAGTTAAAATGGACGATCAATTACGTATCTGTAAATAAGGTTGAAAAATa                                                                                                                                                                                                                     >  2:356523/1‑250 (MQ=255)                     gAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCGGTATGGTGTGATGTGGGGACTTCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGGTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACTTATCTGGACATAAGGTTGAAAAATaa                                                                                                                                                                                                                    <  2:86822/251‑1 (MQ=255)                               cgCAACAGCATGTTGTTTTCCCTCTCGCTGTTGTTGCGGGATGGGGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCt                                                                                                                                                                                                          <  2:629753/250‑1 (MQ=255)                                                  cccTCGCGCTGTTGTTGCTGGATGGGGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTCTCTGGAAATAAGGTTGAAAAATAACACCGGCGCTAATAAGCGCCGtttttttt                                                                                                                                                                                       >  2:120998/1‑251 (MQ=255)                                                          cTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtgg                                                                                                                                                                               >  1:144326/1‑251 (MQ=255)                                                          cTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtgg                                                                                                                                                                               >  1:107832/1‑251 (MQ=255)                                                           tgttgtTGCTGGATTGAGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCCCTAAAAAGCGCCGTTTTTTTTGACggtgg                                                                                                                                                                               >  2:484887/1‑250 (MQ=255)                                                               tttgcaggatggaggggtgtgGGGACTGCTCAACTCTGCCGCTAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGGGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGAATGCGGTTTCAATGACGAAAATGAAAGCCAGTAAAGAAGTTACAAGGGCCGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGACGATAAAAAGCGCCGTTTTTTTTGAAGGTGGTaaa                                                                                                                                                                           >  2:633477/18‑250 (MQ=255)                                                                  gCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCg                                                                                                                                                                       >  1:167574/1‑251 (MQ=255)                                                                       atggtgtgtcgtgtgtacgtcTCAACCCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTTCTGGTGGTGGCGCTGGACGGCATGATGTTTGTGACTCGTAATATCGACTGTTATGTGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAAGTACGGATCTGGAAATAAGGTTGAAAAATAAAAACTTCGCTAAAAAGCGCCGTTTTTTTTGACGGTTGTAAAGCTGATTa                                                                                                                                                                   <  2:598895/230‑1 (MQ=255)                                                                           tgtgATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCtt                                                                                                                                                              >  1:411397/1‑251 (MQ=255)                                                                                            ccaattctGCCGAGAGCGCGGTGTTGTGGGGAACCAGTGTGCTGGTGGTGGCGCTTGCCGGCATGATGTTTGTGAATCGTAATATCGACTGGTAAGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGCCGATCAATTTCGTAGCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGGTTTTGTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCTCCACAGAAg                                                                                                                                             <  2:428324/246‑1 (MQ=255)                                                                                                    gCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAAcc                                                                                                                                     <  1:41578/251‑1 (MQ=255)                                                                                                           gcgcgcTTTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCg                                                                                                                              <  1:474040/251‑1 (MQ=255)                                                                                                                            actagtgttgtgggggtggtGCTGGCCGGCAGGATGTTTGTGCAGCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCtatgat                                                                                                             <  2:171693/237‑1 (MQ=255)                                                                                                                               attgtgCTGGGGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCTAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACTGCGCTAAAAAgc                                                                                                                                                                                                   <  2:494020/160‑1 (MQ=255)                                                                                                                               aGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAACTAAGGTTGAAAAATAAAAACGGCGCTAAAAAgc                                                                                                                                                                                                   >  1:494020/1‑162 (MQ=255)                                                                                                                                  gtgCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccgg                                                                                                       <  1:42172/251‑1 (MQ=255)                                                                                                                                         tggtggCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccggcgtatcc                                                                                                >  1:15708/1‑251 (MQ=255)                                                                                                                                                                      actcGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcg                                                                   >  1:280951/4‑251 (MQ=255)                                                                                                                                                                        tcGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcggc                                                                 >  1:613519/2‑251 (MQ=255)                                                                                                                                                                         cGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcggcg                                                                <  1:262603/251‑1 (MQ=255)                                                                                                                                                                             atatCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATc                                                            <  2:641644/251‑1 (MQ=255)                                                                                                                                                                                 cGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTc                                                         <  2:167574/250‑1 (MQ=255)                                                                                                                                                                                    cTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGAGGCGATGATTTCCGGCGTATCAGGCGTAGCTTCGAATTGTTTACGAATACGGCGGACCGTccacc                                                     >  2:499732/1‑247 (MQ=255)                                                                                                                                                                                       gTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCt                                                   >  1:244225/1‑250 (MQ=255)                                                                                                                                                                                            cGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAACTACGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCCGATCACCACCGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAGTGTTTACGAATACGGCGGATCgcaccggctcccgt                                              >  2:241149/1‑237 (MQ=255)                                                                                                                                                                                               tttCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACg                                           <  1:207851/250‑1 (MQ=255)                                                                                                                                                                                                      gCCGAAAATGAAAGCCAGGAAAGAAGTTACAATGGACGATTAATTACGTATCTGGAAATAAGGGTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGACTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGAGTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAAGACGGCGGATCGTCACGTCTACAGTACGGTCGTgtg                                   <  2:218694/251‑3 (MQ=255)                                                                                                                                                                                                        cGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGt                                 <  1:209645/251‑1 (MQ=255)                                                                                                                                                                                                          aaaaTGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCAACGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTCCAGTCGTGAGGttt                               >  1:655240/1‑251 (MQ=255)                                                                                                                                                                                                            aaTGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGCTCGTCACGTCTACCGTACGGTCGTGTGGTTTc                              >  1:680361/1‑250 (MQ=255)                                                                                                                                                                                                              tGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGGTTCGAAATGTTTACGAATACGGCGGATCGTCACCTCTACAGTACGGGTGTGGGGTTTCatg                           >  2:355967/1‑249 (MQ=255)                                                                                                                                                                                                              tGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGGGTGGTTTTAGc                           >  2:231789/1‑251 (MQ=255)                                                                                                                                                                                                                aaaGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGCAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGTTGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACCATACGGTcagtgggttttaaccc                         >  2:158408/1‑236 (MQ=255)                                                                                                                                                                                                                 aaGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCa                        <  1:356523/251‑1 (MQ=255)                                                                                                                                                                                                                 aaGCCAGTAAAGAAGGTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCa                        <  2:613519/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTCAAAAGCGCCGTTTTTTTTGACGGTGGAAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCAAGTCAACAGTACGGTCGTGTGGATTCAGCTAACGGCCGGTCATTttctttggcc  >  1:430142/1‑246 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  ATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCA  >  NC_000913/4639273‑4639753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑