Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,217	C→T	T347I (ACC→ATC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,217	0	C	T	100.0%	107.3 / NA	35	T347I (ACC→ATC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/14);  total (21/14)

CTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCG  >  NC_000913/4638971‑4639461                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCCACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGt                                                                                                                                                                                                                                                   >  1:641437/1‑251 (MQ=255)             aTACCGTCTGGGAAACCTTCCCCGCTTTTGGCTGCGCAGTGACGACTCCAGCCGATCAATACCAATTAACGGACCGGGCTACTAAGTACGCCATTCTGCTGATTGCACTGACTGTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTGACACCCAATGCAATATTTGCTGGTGGGGCTTTCAGTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCa                                                                                                                                                                                                                                       <  2:76486/251‑1 (MQ=255)                 cGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGa                                                                                                                                                                                                                                   <  1:614152/251‑1 (MQ=255)                      gggAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATc                                                                                                                                                                                                                              <  1:430129/251‑1 (MQ=255)                       ggAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCg                                                                                                                                                                                                                             >  1:638004/1‑251 (MQ=255)                                             gTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAAt                                                                                                                                                                                                       <  1:93008/251‑1 (MQ=255)                                                     cacgagcccAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGta                                                                                                                                                                                               <  2:638004/244‑1 (MQ=255)                                                             cAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTACACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTACTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCGTTCTGATTGGAGCGTTAATGAACGGAATTTATTTg                                                                                                                                                                                       >  1:379025/1‑251 (MQ=255)                                                                     cAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGt                                                                                                                                                                               >  1:12310/1‑251 (MQ=255)                                                                                 acggACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAACCGCTAACCGAGCGACGTTTAAACCCAATGTAATATTTGCTGGTGGGGCTTTCATTGGGTATGTTTTATTTGATCTTGCTGGCGCTTTCTGAACATATCGGTTTTTCCGAGGCAGGGATAATCGACAGTCTTATTGGGGCGTTAATGCACGGGatttatttacagggggtttttgaaagcgg                                                                                                                                                                   >  2:262603/4‑230 (MQ=255)                                                                                   ggACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTggg                                                                                                                                                                 >  1:598895/2‑250 (MQ=255)                                                                                       cGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGGGCa                                                                                                                                                              >  1:629753/1‑250 (MQ=255)                                                                                               aaaGTACGCTATGCGGCGGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGTTCCCCGCGCAACGTTTACACCCAATGCAAGTTTTGCTGGTTGGGCTTTCATTGGTGATGTTTTATTTGTTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCAtgt                                                                                                                                                    <  2:614657/250‑1 (MQ=255)                                                                                                   tACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTTGCGCAACA‑GCATgttgtt                                                                                                                                                >  1:171693/1‑251 (MQ=255)                                                                                                    aCGCCAGTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGttt                                                                                                                                               <  1:236609/251‑1 (MQ=255)                                                                                                                    tGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTTTTGGCAGGCTGGCGCAACA‑GCATTTTGTTTACACTctggctgtggt                                                                                                                               >  1:176875/1‑241 (MQ=255)                                                                                                                        cTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCTCCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGTGCTTTCAGTTGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGttgttg                                                                                                                             <  2:641437/249‑1 (MQ=255)                                                                                                                           aCTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGGGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGCTTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGCAAGGCCGGCGCG‑CACGCAAGTTGTTTTCCCTCGCGCTGTTGTTGCTggg                                                                                                                        >  1:640324/1‑250 (MQ=255)                                                                                                                              tttATGGCGTGCTTTTTGTTTGAAACGCTGTCCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATTTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATg                                                                                                                      <  2:400170/250‑1 (MQ=255)                                                                                                                                       ttCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGGAAGGCTGGCGCACCA‑GCCTGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGGGTGAtttg                                                                                                            >  1:218694/1‑248 (MQ=255)                                                                                                                                             gTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGGAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACt                                                                                                      >  1:344289/1‑251 (MQ=255)                                                                                                                                                     aaCGCTAACCGCGCAACGTTTACACCCAATGCAATATTTGCTAGTGGAGCTTTCATAGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGTTTAGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTTAAATGCTGGCGGAACA‑GCATGTTTTTTTCCCCAGAGCTGTTGTTGATTGTGGGGGTTGTGTGGGGACTGCTCaaac                                                                                              >  2:524876/1‑249 (MQ=255)                                                                                                                                                      aCGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGGTGTGGGGGCTGCTCAActc                                                                                             >  1:428324/1‑251 (MQ=255)                                                                                                                                                      aCGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGAtgtg                                                                                                            <  1:140393/236‑1 (MQ=255)                                                                                                                                                      aCGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGAATTTAAAGGCTGGCGCAACA‑GACTGTTGTTTACCCTCGCGCTGTTGTTGCTGGGTGGGGTGAtgtg                                                                                                            >  2:140393/1‑236 (MQ=255)                                                                                                                                                        gCTCACCGCGCAACGTTTCCTCCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTg                                                                                           <  2:12310/251‑1 (MQ=255)                                                                                                                                                                cgcAATGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCCGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACC‑GGATATTGTTTTCCCTCGCGCTGTTGTTGATGGATGGTGTGATGTGGGGCCTGCTCAACTCTGCCGAtcgc                                                                                   >  1:111975/1‑248 (MQ=255)                                                                                                                                                                                        atTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGtgtg                                                           >  1:595851/1‑251 (MQ=255)                                                                                                                                                                                        atTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGGGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTggggaaccgtgtg                                                           >  1:358760/1‑241 (MQ=255)                                                                                                                                                                                                              ggTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtattta                                                                                                                                                                                           <  2:508298/102‑1 (MQ=255)                                                                                                                                                                                                              ggTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtattta                                                                                                                                                                                           >  1:508298/1‑102 (MQ=255)                                                                                                                                                                                                                  aTGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCatgat                                 <  1:370782/251‑1 (MQ=255)                                                                                                                                                                                                                    gTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTTCCCTCGCGCTGTTGTTGCTGGGTGGTGTGATGTGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGGACCCGTGTGCTGGTGGGGGCGCTGGCCGGCATGgtgt                               >  1:274055/1‑251 (MQ=255)                                                                                                                                                                                                                       gtATTTGCTCTTGCTGTCGCTTTCTGAACATATCGGTTTGACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGttt                             <  2:595851/249‑1 (MQ=255)                                                                                                                                                                                                                                                 aaCATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGGTGGTGTGATGTGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGGCTGGTATGCg  >  1:127403/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACA‑GCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCG  >  NC_000913/4638971‑4639461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑