Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,598	A→G	V121A (GTC→GCC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,598	0	A	G	100.0%	148.5 / NA	50	V121A (GTC→GCC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/21);  total (29/21)

CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTT‑CTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGG  >  NC_000913/7355‑7837                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACCGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACg                                                                                                                                                                                                                                            >  2:571211/1‑251 (MQ=255)                aGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCACCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAgctg                                                                                                                                                                                                                            <  1:148519/251‑4 (MQ=255)                            atcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGGCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCAGCACTTT‑CTTTATAAAGCTGTGCGAGGGAACa                                                                                                                                                                                                                <  1:76969/248‑1 (MQ=255)                              cgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACAt                                                                                                                                                                                                               <  1:563648/249‑1 (MQ=255)                               gCCAGCAGAGCAAAGACAGCGAGAATAATACCTATCACCAGCGGGGGAAAATCAACAGAAAAACTCAGGGCTCGCGCAACGGCGATCGCCTGAATTCCGCTGAAAATTATGACGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATACCCAGCCCGCGCGCCATATACCATGCCGTTCCGACACGCAACTGCCCATTGGCATACCGAT‑TTTTATAAAGCTGTGCGAGGGAACATTc                                                                                                                                                                                                             >  2:676572/1‑251 (MQ=255)                                 cagcagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCgg                                                                                                                                                                                                           >  1:274353/1‑251 (MQ=255)                                       agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGCCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCaaa                                                                                                                                                                                                       >  1:283527/4‑249 (MQ=255)                                         caaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACg                                                                                                                                                                                                     >  1:444787/2‑249 (MQ=255)                                          aaaGACAGCGAGAATAATAACTGTCAACAGCGGGGGAAAGTCAAAAGAAAAACTCAGGGATCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAAAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCAATTGGCATCACGTT‑ATTTATAAAGCTGTGCGAGGGAACCTTCGGCAAACGAgg                                                                                                                                                                                                  >  1:588138/1‑251 (MQ=255)                                                aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc                                                                                                                                                                                             >  1:675250/1‑250 (MQ=255)                                                    agaATAATACCTGTCAACAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGAGCAACGGCGGTCGCCTGAATTCCGCTGAAAATTATGCAGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCAGCCACGACACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGCACATTCGGAAAACGAGGTCGcactgcc                                                                                                                                                                                        >  2:468907/1‑245 (MQ=255)                                                       ataataCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCACGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGCGGTCGCCATTCCGa                                                                                                                                                                                      >  2:330703/1‑250 (MQ=255)                                                         aataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                    >  1:617306/5‑250 (MQ=255)                                                            accTGTCACCAGCGGGGGAAAATCAAAAGGAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAGTTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGATGAGCGAGGGAACATTCGGCAATCGAGGCCGCCATGCCGATAAAcg                                                                                                                                                                                >  2:46497/2‑251 (MQ=255)                                                               ggTCACCAGCGGGGGAAAAGCAAAAGAAAAACGCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCgg                                                                                                                                                                             <  1:86498/250‑1 (MQ=255)                                                                 tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGc                                                                                                                                                                            >  1:140285/1‑250 (MQ=255)                                                                 tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGGCGCCCTGCCGATAAACGCGGc                                                                                                                                                                            >  2:508498/1‑250 (MQ=255)                                                                         gggggAACATCAAACGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGCAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCCGGTCCGCCCCGCAACTGCCCCTGGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGACAAACGAGGACGCCCTGCCGATAAACGCGGCCACCcacac                                                                                                                                                                   >  2:154775/1‑250 (MQ=255)                                                                              aaactCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCCCGCAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGCAGCCCTGCAGATAAACGCGTAAACCCACATcc                                                                                                                                                                 >  2:195973/5‑248 (MQ=255)                                                                                   cAAAAGAAAAACTCAGGGCACGCGAAACGGCGTTCGCTTGAAGTCCGCTGAAAATTATGCCGCAGGCGATGAGCAAAAAGACGGCGAAAAGAACCCCCATCCAGCGCACCCCCAGCCCGCGCGCCTTATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGAc                                                                                                                                                          <  2:58691/250‑1 (MQ=255)                                                                                    aaaaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGCGCAAAAGGACGGCGAACAGAACGCCCCTCCAGCGCATCACCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGACACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGTGGTCGCCATGCCGCTAAACGCAGCAACCCACATCCAGAAGACgg                                                                                                                                                        >  1:320716/1‑251 (MQ=255)                                                                                                         cccaccggcgtccgcctaaatcccGCTAAAAATTAGGCCGTGGGCGATGCGCAAAAAGCCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTaa                                                                                                                                   <  2:672594/229‑1 (MQ=255)                                                                                                           cAACGGCGTTCGCCTGAATTCCGATGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAAATCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGGGGACGCCATGCAGTTAAACGCGGAAACCCACATCCAGACGACTGCCACAGGTCCACccgcggcaaat                                                                                                                                 >  2:657629/1‑246 (MQ=255)                                                                                                            aaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCACCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAttg                                                                                                                                <  1:334784/251‑3 (MQ=255)                                                                                                            aaCGGCGTTCGCCTGAATCCCGCGGAAAATTATGCCGTAGGCGATGACCAAAAAGACGGCGAACAGAACCCCCATCCAGCGCTCCCCCAGCCCGCGCGCCATATCCCAGCCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAttg                                                                                                                                <  1:263093/251‑3 (MQ=255)                                                                                                                  gatCGCCTGAATTCCGCTGAAAATTATGCCGAAGGCGATGCGCAAAAAGACGGCGAACAGCACGCCCAACCGGCGCATCCCCAGCCCGCGCGCCATATACCATGACGGGCCGCCACGAAACTTCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTAGGCAACAGAAGTAGCCCTGCCGAACAACGCGACAACACACATACAGAAGCAGGCTCCACATCCACcgccgggaaaatacagc                                                                                                                           >  2:376219/3‑239 (MQ=255)                                                                                                                       cctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTTCCCGCGC‑AAcc                                                                                                                    >  1:382952/3‑250 (MQ=255)                                                                                                                       cctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAAtttcccacgcaacc                                                                                                                     >  1:632394/3‑238 (MQ=255)                                                                                                                          gaattCCGCCGAAAATTATTCCGTCGGCGATGATCAAAAAGCCGGCGAACAGAACCCCCATCCAGCTCATCCCCAGCCCGCGCGCCATATCCCAAGCCGGGCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCTAGCTC‑AACGCCg                                                                                                                 <  1:166098/247‑1 (MQ=255)                                                                                                                                       aaTTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGGTGCCGc                                                                                                    >  1:67150/1‑251 (MQ=255)                                                                                                                                        aTTATGCCGTAGGCAATGAGAAAAAAGCCGGCGAACAGACCGCCCAGCCAGCGCATCCCCAACCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCAGTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGACGCCCTGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCt                                                                                                   <  1:46497/251‑1 (MQ=255)                                                                                                                                                     cGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGcgcc                                                                                      <  1:154775/251‑1 (MQ=255)                                                                                                                                                      gATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGACC‑AACGCCGGCCAGGGTGCCCCCACCCACGCGcgccc                                                                                     >  1:284770/1‑250 (MQ=255)                                                                                                                                                                                 ccccctCCAGTGCACCCCCAGCCCGCGCGCCATATCCCACTCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAACCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATg                                                          <  1:565219/246‑1 (MQ=255)                                                                                                                                                                                      ccccgccaacccccgccccGCGCCCCATATCCCATCACGGTCCGCCAAGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAGTGCCAGCGC‑AACGCCGGCCAGGGTGCCGCGACCCACGCGCGTCGCAAGACTGGTACACAATGACTGAAATGAGGtt                                                     <  2:544714/235‑1 (MQ=255)                                                                                                                                                                                       ccAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGC‑AACGCCCGCCCGGGTGCCGCTACCCACGCGCGCCGCCATGCTGGGACACAATGaagatcggaagcgcc                                                    >  1:480280/1‑237 (MQ=255)                                                                                                                                                                                           caccacccccccccGCGCCCCATATCCCATGCGGGTCCGCAACAAAACTGCCCATTGGCACCACGTG‑CTTTATAAAGCTGTGGGAGGGACCATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAGCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAAcc                                                <  2:629151/240‑1 (MQ=255)                                                                                                                                                                                            gcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCCACGC‑AACGCCGGCCCGGGTGCCGCTAACCACGCGCGCCGCCAGACTGGGACACAAAGACTGAAATGAGGTTaaaacc                                               >  1:20508/1‑248 (MQ=255)                                                                                                                                                                                                   cAGCCCGCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGTTACACAATGACTGAAATGAGGTTAAACCGCCTGGCt                                        <  1:571211/251‑1 (MQ=255)                                                                                                                                                                                                     ccccgcgccccaaaacacATGCCGGTCCACCACAAAGCTGCCCGTTGGCATCA‑GTTGCTTTATAAGGCGGTGCGGGGGAACATCCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCACCCCACATCCAGAAGACGGCACCAGGTCAACCGGCGGTAATTGCCAGCGT‑AACGCCGGCCAGGTGGCCGCTCCCCACGCGCGCCGAAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCtgt                                      <  2:107214/234‑1 (MQ=255)                                                                                                                                                                                                              ccATATCCCATGCCGGCCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATg                             <  1:603899/251‑1 (MQ=255)                                                                                                                                                                                                               cATAGACCATGCCGGGCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGAc                                                                               <  1:86826/200‑1 (MQ=255)                                                                                                                                                                                                                  ataCCATGCCGTCCCCCCACGAACCTGCCCATGGGCATCACGTT‑CTTAATAAACCGGTGCGAGGTACCATCCGCCAAACGAGGTCGCCTTGCCGATAAACGCGGCAACCCACACCCAGAATACGGCTCCAGGTCCACCGGCGGTAATGGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGTAAGACTTGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTAt                         <  1:340879/251‑1 (MQ=255)                                                                                                                                                                                                                      cATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGAAAATTCCAGCGCCAAC‑CCGGACAGGGTGCCGCCACCCCCACGGCCCGCAAGGCTGGGCAACACTGAATGAAATGAGGGTAAACCCCCTGGCTGTGGATGAATGCTCttttt                     >  2:415830/1‑251 (MQ=255)                                                                                                                                                                                                                           cGGTCCGCCACGAACCTGCCCATTGGCATCCCTTC‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGAc                <  1:144357/251‑1 (MQ=255)                                                                                                                                                                                                                           cGGTCCGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTa                                                    <  1:668087/215‑1 (MQ=255)                                                                                                                                                                                                                                cGCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGAAATTGCCAGCGC‑AAAGCCGGCCAGGATGCCGCTACCCACGCGCGCCGCCAGACTGGTACACCATGACTGGAATGAGGGTAAACCGCCTGGCTTTGGGTGTATGCTATTTTTAAggcctttg           >  1:480929/1‑244 (MQ=255)                                                                                                                                                                                                                                 gCCACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCTCGC‑AACGCCGGCCAGGGTGCCGCCACCCCCCCGCGCCGCATGACTGGGACACAATGGCTGAACAGAGGTTAAACCGCACGGCTTGGGATGAATGATCTTTTTAAAACtttttc          >  2:526969/1‑249 (MQ=255)                                                                                                                                                                                                                                   cACGAAACTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGC‑AACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGGACACCATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGGTGAATGCTATTTTTAAGACTTTTGccc        >  1:364508/1‑250 (MQ=255)                                                                                                                                                                                                                                         aCTGCCCATTGGCATCACGTT‑CTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCAGCGC‑AAAGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCAAGGCTGGTACACAATGACTGAAATGAGGTTAAACCGCCGGGGTGTGGGAGAATGCTATTTTTAAACCTTTTGCCAAACTgg  >  1:227743/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTT‑CTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGC‑AACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGG  >  NC_000913/7355‑7837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑