Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,600	A→C	P141P (CCA→CCC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,600	0	A	C	95.5%	134.8 / ‑3.5	44	P141P (CCA→CCC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (20/22);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAAC  >  NC_000913/4638368‑4638845                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tgACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGAAGCGGAAACGCTGTTTTATCCAGTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGAGAGGGATTTAAACCGGTCAGGTCTGGCACAGTGCTTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAATGCACCACATATCATCTTAGGAACGCACTTTATTGTGATTAGCGTc                                                                                                                                                                                                                                     >  2:357736/1‑251 (MQ=255)    ccGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCggg                                                                                                                                                                                                                                  >  2:577543/1‑251 (MQ=255)    ccGAGCTTTATACGGTGAAGGTAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTGACCTGAGGCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGTGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCATTTATTGTGATTAGCGTCggg                                                                                                                                                                                                                                  <  2:294568/251‑1 (MQ=255)      gAGCTTTATTCGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAAATAAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATTCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGa                                                                                                                                                                                                                                <  2:485521/251‑1 (MQ=255)                 ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCggcg                                                                                                                                                                                                                                    <  2:82922/236‑4 (MQ=255)                 ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCg                                                                                                                                                                                                                                    >  1:82922/1‑236 (MQ=255)                     cAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATtgg                                                                                                                                                                                                                 >  2:282900/1‑251 (MQ=255)                                 aaaaCCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAgc                                                                                                                                                                                                     >  1:18843/1‑251 (MQ=255)                                                  aCGAAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaacggaa                                                                                                                                                                                    <  2:201852/251‑1 (MQ=255)                                                    gAAGTTTTATCCATTTTTGGTTAACTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGCTAGGGATTTATACCGGTCAGGTCTGGCAAAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGACAAATATTATCTTAGGCAAGCCCTATATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGAGCCTGAAGTTaacggcacg                                                                                                                                                                                  >  1:162306/1‑251 (MQ=255)                                                     atgTTTTATCTATTTGTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCTCTTTATTGTGATGTGCTTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaatggaacgg                                                                                                                                                                                 <  2:102153/249‑1 (MQ=255)                                                                      ggTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAccc                                                                                                                                                                >  1:153704/1‑251 (MQ=255)                                                                      ggTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAccc                                                                                                                                                                <  1:563015/251‑1 (MQ=255)                                                                      ggTTACCTGAGGCATTGATGGTTGATGGCAAGCAGAACGTGGAAGAACGCAATAGAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGTTGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGGTAACGGAACGGCGCTGATCATTGAAcc                                                                                                                                                                 <  2:164429/250‑1 (MQ=255)                                                                          aCCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGc                                                                                                                                                             <  1:631852/250‑1 (MQ=255)                                                                                gTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGt                                                                                                                                                      >  1:404862/1‑251 (MQ=255)                                                                                                ggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGGACCCGGCACCGGGTTAGAGCAAGGCGGGc                                                                                                                                       >  2:112640/1‑250 (MQ=255)                                                                                                    aTCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGc                                                                                                                                  <  1:282900/251‑1 (MQ=255)                                                                                                       aGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTg                                                                                                                               <  1:600655/251‑1 (MQ=255)                                                                                                                                 aTTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCTTGGTGTGGTGAAAGCGCCTGAAGTTACAGGAACGGCGCTGACCATTGAACCCGGCCACGGGGTAGAGCAAGGCGGGCAAGGCGTGCATAACCCTTTACCCGCAGGGGGCTg                                                                                                     >  2:379258/1‑251 (MQ=255)                                                                                                                                    tataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGc                                                                                                   <  1:180003/250‑1 (MQ=255)                                                                                                                                       aCCGGTCAGGTCTGGCACAGTTATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGAGAGCTCAACGCGCAAAATATCATCTTAGGCAAGCCCTTTATTGAGATTAGCGTCGGGGAGGCGCGGGGTATTGGTGGGGTGAAAGCGCCTGAAGTCAACGTAAAGGCGCTGACCATTGAACACGGCCCCGGGTTAGAGCAAGGCGGGGCGGGGGGGCATATCCCTTCACCCGAAaggggcatgagggc                                                                                               >  2:4996/1‑241 (MQ=255)                                                                                                                                          ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                                                                                               <  2:291215/152‑1 (MQ=255)                                                                                                                                          ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                                                                                               >  1:291215/1‑152 (MQ=255)                                                                                                                                          ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGGAGc                                                                                             >  1:14395/1‑250 (MQ=255)                                                                                                                                              aGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAAc                                                                                        <  1:380662/251‑1 (MQ=255)                                                                                                                                                    ggCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGATAACGGAACGGCGCTGACCATTGAACCCGGCCCCGGGGTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGGCTGGCGGAAGCAAAACCTg                                                                                     >  2:219591/1‑248 (MQ=255)                                                                                                                                                         aGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCgg                                                                                                                                                              >  1:429680/1‑170 (MQ=255)                                                                                                                                                         aGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCgg                                                                                                                                                              <  2:429680/170‑1 (MQ=255)                                                                                                                                                             aTTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCGGGGCGTGCATATCCCTTTACCCGCAGGGGACTGGCgggagaggaaacttaaccgtaatatg                                                                         >  2:477872/1‑227 (MQ=255)                                                                                                                                                                  acgctAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCt                                                                    <  2:406772/247‑1 (MQ=255)                                                                                                                                                                         aaGCCTATTGCGATGGTACGCGTCTGAGCGAGCTCAACGCTCCAAATTTCATTTGAGGCAAGCCCTTGATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATAGCCCGTGAACCGAAGGTGACTGGCGGAAGCAGAACCTGAAGAGGAATATGGCCCTGAATTTa                                                             <  2:673996/251‑1 (MQ=255)                                                                                                                                                                         aaGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAACGTCGGGGAAGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAAAGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATAACCCTTTACCCGAAGGGGACTGGCGGCAGAAGAACAGGAAGCTGAATATGGCCATGGAttt                                                              >  1:415980/1‑250 (MQ=255)                                                                                                                                                                            ccGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGc                                                          <  1:453262/251‑1 (MQ=255)                                                                                                                                                                                 ttccaagTTTCGCCTCTTTGCGAGCTCAACTCGCCAAATATCTTCTTAGGCAAGCCCTTTATTTTGATTAGCGTCGGTGATGCGCGTGGTATTGGTGTGGTGAAAGCTCCTGAAGTTAACGGCACGGCGCGGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCTTGCATATCCCTTTACCCGAAGGTGACTGGCGGAAGCATATCCGGAAGCTGAATAGGGCCCTGAATTTAAGCGGTa                                                      <  2:335489/244‑1 (MQ=255)                                                                                                                                                                                    gATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGTGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCgg                                                  <  1:4996/251‑1 (MQ=255)                                                                                                                                                                                             cgTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGACGAAGAACCTGCAGCTGAATATGGCCCTGAATTTTAGCGGGACCGGCGAttcttt                                         >  1:384571/1‑246 (MQ=255)                                                                                                                                                                                             cgTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCGTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTc                                         <  1:647741/251‑1 (MQ=255)                                                                                                                                                                                                  tAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTTGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtg                                       <  1:369662/248‑1 (MQ=255)                                                                                                                                                                                                        gcttaaCGCGCAAAATATCATCTTATGCAAACCCTTTATTGTGATTAGCGTAGGGGATGCGCGGTGTATTGGTGGGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGACCCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATTGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGTTGCCTg                              <  2:536789/247‑1 (MQ=255)                                                                                                                                                                                                            aaCGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGGATCAGAAACTTGAGCTGAATAGGACCCCGAATTTAAGCGGGACCGGCgctcgtttttttggtgcagccgg                          >  2:222857/1‑229 (MQ=255)                                                                                                                                                                                                              cgcgCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGGATTTAAGCGGTACCGGCGATCTTTCTGTGGGGACTgccgggc                        >  1:510358/1‑251 (MQ=255)                                                                                                                                                                                                                  ccAAATATCATCTTAGGCAAGCCCTTTATTGTGACTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGaa                                                                                                             <  1:151548/162‑1 (MQ=255)                                                                                                                                                                                                                                    aaGCCATTTATTGTGATTAGCGCCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAACGGTACCGGCGATCTTTCTGTGGGGCCTGGCGGGCGTAATAGCGAAATTACCTTAAc  >  1:283632/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAAC  >  NC_000913/4638368‑4638845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑