Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,587	C→T	T137I (ACC→ATC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,587	0	C	T	100.0%	144.2 / NA	46	T137I (ACC→ATC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

GTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGC  >  NC_000913/4638343‑4638823                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             tttGGGCCGCTTATCGCTATTCCTGTGACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGTGTTTTATACCGGTCAGGTCTGGCACAGGGATTTTACGCTAAAAGCCGCTTTCGATGTTTCGCGTCTTAGCGAGCACAACGCTACAAATATAATCTTAgg                                                                                                                                                                                                                                        >  2:132353/2‑251 (MQ=255)         gcttaTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGccct                                                                                                                                                                                                                                <  1:262900/247‑3 (MQ=255)            ttttgctcttcttgtgACCGAGATTTGTACGTTGCAGGAAGAGGATAAATCCGTGGATCGGAAACGAAGGTTTATCAATTTTTGGTTACCTGAGTCATTGATGGTTGATTGCAATCAGAACGTGGTAGAACTCAAGATAGGGATTTATACCGGTCAGGTCTGGCACTGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGTGCTCAACGCGCCAAAGATCATCTTTTGCAAGctcttt                                                                                                                                                                                                                              <  2:146983/238‑6 (MQ=255)                          tgACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGAAGCGGAAACGCTGTTTTATCCAGTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGAGAGGGATTTAAACCGGTCAGGTCTGGCACAGTGCTTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAATGCACCACATATCATCTTAGGAACGCACTTTATTGTGATTAGCGTc                                                                                                                                                                                                               >  2:357736/1‑251 (MQ=255)                             ccGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCggg                                                                                                                                                                                                            >  2:577543/1‑251 (MQ=255)                             ccGAGCTTTATACGGTGAAGGTAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTGACCTGAGGCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGTGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCATTTATTGTGATTAGCGTCggg                                                                                                                                                                                                            <  2:294568/251‑1 (MQ=255)                               gAGCTTTATTCGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAAATAAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATTCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGa                                                                                                                                                                                                          <  2:485521/251‑1 (MQ=255)                                          ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCggcg                                                                                                                                                                                                              <  2:82922/236‑4 (MQ=255)                                          ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCg                                                                                                                                                                                                              >  1:82922/1‑236 (MQ=255)                                              cAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATtgg                                                                                                                                                                                           >  2:282900/1‑251 (MQ=255)                                                          aaaaCCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAgc                                                                                                                                                                               >  1:18843/1‑251 (MQ=255)                                                                           aCGAAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaacggaa                                                                                                                                                              <  2:201852/251‑1 (MQ=255)                                                                             gAAGTTTTATCCATTTTTGGTTAACTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGCTAGGGATTTATACCGGTCAGGTCTGGCAAAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGACAAATATTATCTTAGGCAAGCCCTATATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGAGCCTGAAGTTaacggcacg                                                                                                                                                            >  1:162306/1‑251 (MQ=255)                                                                              atgTTTTATCTATTTGTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCTCTTTATTGTGATGTGCTTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaatggaacgg                                                                                                                                                           <  2:102153/249‑1 (MQ=255)                                                                                               ggTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAccc                                                                                                                                          >  1:153704/1‑251 (MQ=255)                                                                                               ggTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAccc                                                                                                                                          <  1:563015/251‑1 (MQ=255)                                                                                               ggTTACCTGAGGCATTGATGGTTGATGGCAAGCAGAACGTGGAAGAACGCAATAGAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGTTGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGGTAACGGAACGGCGCTGATCATTGAAcc                                                                                                                                           <  2:164429/250‑1 (MQ=255)                                                                                                   aCCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGc                                                                                                                                       <  1:631852/250‑1 (MQ=255)                                                                                                         gTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGt                                                                                                                                >  1:404862/1‑251 (MQ=255)                                                                                                                         ggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGGACCCGGCACCGGGTTAGAGCAAGGCGGGc                                                                                                                 >  2:112640/1‑250 (MQ=255)                                                                                                                             aTCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGc                                                                                                            <  1:282900/251‑1 (MQ=255)                                                                                                                                aGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTg                                                                                                         <  1:600655/251‑1 (MQ=255)                                                                                                                                                          aTTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCTTGGTGTGGTGAAAGCGCCTGAAGTTACAGGAACGGCGCTGACCATTGAACCCGGCCACGGGGTAGAGCAAGGCGGGCAAGGCGTGCATAACCCTTTACCCGCAGGGGGCTg                                                                               >  2:379258/1‑251 (MQ=255)                                                                                                                                                             tataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGc                                                                             <  1:180003/250‑1 (MQ=255)                                                                                                                                                                aCCGGTCAGGTCTGGCACAGTTATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGAGAGCTCAACGCGCAAAATATCATCTTAGGCAAGCCCTTTATTGAGATTAGCGTCGGGGAGGCGCGGGGTATTGGTGGGGTGAAAGCGCCTGAAGTCAACGTAAAGGCGCTGACCATTGAACACGGCCCCGGGTTAGAGCAAGGCGGGGCGGGGGGGCATATCCCTTCACCCGAAaggggcatgagggc                                                                         >  2:4996/1‑241 (MQ=255)                                                                                                                                                                   ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                                                                         >  1:291215/1‑152 (MQ=255)                                                                                                                                                                   ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                                                                         <  2:291215/152‑1 (MQ=255)                                                                                                                                                                   ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGGAGc                                                                       >  1:14395/1‑250 (MQ=255)                                                                                                                                                                       aGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAAc                                                                  <  1:380662/251‑1 (MQ=255)                                                                                                                                                                             ggCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGATAACGGAACGGCGCTGACCATTGAACCCGGCCCCGGGGTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGGCTGGCGGAAGCAAAACCTg                                                               >  2:219591/1‑248 (MQ=255)                                                                                                                                                                                  aGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCgg                                                                                                                                        >  1:429680/1‑170 (MQ=255)                                                                                                                                                                                  aGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCgg                                                                                                                                        <  2:429680/170‑1 (MQ=255)                                                                                                                                                                                      aTTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCGGGGCGTGCATATCCCTTTACCCGCAGGGGACTGGCgggagaggaaacttaaccgtaatatg                                                   >  2:477872/1‑227 (MQ=255)                                                                                                                                                                                           acgctAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCt                                              <  2:406772/247‑1 (MQ=255)                                                                                                                                                                                                  aaGCCTATTGCGATGGTACGCGTCTGAGCGAGCTCAACGCTCCAAATTTCATTTGAGGCAAGCCCTTGATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATAGCCCGTGAACCGAAGGTGACTGGCGGAAGCAGAACCTGAAGAGGAATATGGCCCTGAATTTa                                       <  2:673996/251‑1 (MQ=255)                                                                                                                                                                                                  aaGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAACGTCGGGGAAGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAAAGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATAACCCTTTACCCGAAGGGGACTGGCGGCAGAAGAACAGGAAGCTGAATATGGCCATGGAttt                                        >  1:415980/1‑250 (MQ=255)                                                                                                                                                                                                     ccGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGc                                    <  1:453262/251‑1 (MQ=255)                                                                                                                                                                                                          ttccaagTTTCGCCTCTTTGCGAGCTCAACTCGCCAAATATCTTCTTAGGCAAGCCCTTTATTTTGATTAGCGTCGGTGATGCGCGTGGTATTGGTGTGGTGAAAGCTCCTGAAGTTAACGGCACGGCGCGGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCTTGCATATCCCTTTACCCGAAGGTGACTGGCGGAAGCATATCCGGAAGCTGAATAGGGCCCTGAATTTAAGCGGTa                                <  2:335489/244‑1 (MQ=255)                                                                                                                                                                                                             gATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGTGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCgg                            <  1:4996/251‑1 (MQ=255)                                                                                                                                                                                                                      cgTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGACGAAGAACCTGCAGCTGAATATGGCCCTGAATTTTAGCGGGACCGGCGAttcttt                   >  1:384571/1‑246 (MQ=255)                                                                                                                                                                                                                      cgTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCGTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTc                   <  1:647741/251‑1 (MQ=255)                                                                                                                                                                                                                           tAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTTGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtg                 <  1:369662/248‑1 (MQ=255)                                                                                                                                                                                                                                 gcttaaCGCGCAAAATATCATCTTATGCAAACCCTTTATTGTGATTAGCGTAGGGGATGCGCGGTGTATTGGTGGGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGACCCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATTGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGTTGCCTg        <  2:536789/247‑1 (MQ=255)                                                                                                                                                                                                                                     aaCGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGGATCAGAAACTTGAGCTGAATAGGACCCCGAATTTAAGCGGGACCGGCgctcgtttttttggtgcagccgg    >  2:222857/1‑229 (MQ=255)                                                                                                                                                                                                                                       cgcgCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGGATTTAAGCGGTACCGGCGATCTTTCTGTGGGGACTgccgggc  >  1:510358/1‑251 (MQ=255)                                                                                                                                                                                                                                           ccAAATATCATCTTAGGCAAGCCCTTTATTGTGACTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGaa                                                                                       <  1:151548/162‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             GTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGC  >  NC_000913/4638343‑4638823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑