Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,567	A→G	E130E (GAA→GAG)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,567	0	A	G	100.0%	151.3 / NA	50	E130E (GAA→GAG)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

GTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTG  >  NC_000913/4638320‑4638808                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  gTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTc                                                                                                                                                                                                                                                >  1:485521/1‑251 (MQ=255) gTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTc                                                                                                                                                                                                                                                <  1:487096/251‑1 (MQ=255)   aCCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCaa                                                                                                                                                                                                                                              <  1:59453/251‑1 (MQ=255)         gggCCGCAAAAACTCGTTAGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGCGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTAGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGCTTTAACGCTAAAAGCCAATTTCGATGTTTAGCGTCTTAGCGAGCTCACCGCGcc                                                                                                                                                                                                                                        >  2:380662/1‑251 (MQ=255)          ggCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCa                                                                                                                                                                                                                                       <  1:29485/251‑1 (MQ=255)                    acgcGTTGGGCCGCTTATCGCTATTCCTGTGTCCGAGCTTTAGTCGGTTCAGGAAGAGGATAAAACTGTGGAGCGGAAACGAAGTTTTATACTTTTTTGGTTACCTGAGTCATTGATTGTTGATGGCAATCAGAACGTGTAAGAACGCAATATAGGTATTTATAACTGTCAGGTCTGGACCAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAtatagcagct                                                                                                                                                                                                                             <  2:29651/248‑7 (MQ=255)                        tttGGGCCGCTTATCGCTATTCCTGTGACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGTGTTTTATACCGGTCAGGTCTGGCACAGGGATTTTACGCTAAAAGCCGCTTTCGATGTTTCGCGTCTTAGCGAGCACAACGCTACAAATATAATCTTAgg                                                                                                                                                                                                                         >  2:132353/2‑251 (MQ=255)                                gcttaTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGccct                                                                                                                                                                                                                 <  1:262900/247‑3 (MQ=255)                                  ttaTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAAcg                                                                                                                                                                                                                                            >  2:477850/3‑222 (MQ=255)                                  ttaTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAAcg                                                                                                                                                                                                                                            <  1:477850/220‑1 (MQ=255)                                   ttttgctcttcttgtgACCGAGATTTGTACGTTGCAGGAAGAGGATAAATCCGTGGATCGGAAACGAAGGTTTATCAATTTTTGGTTACCTGAGTCATTGATGGTTGATTGCAATCAGAACGTGGTAGAACTCAAGATAGGGATTTATACCGGTCAGGTCTGGCACTGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGTGCTCAACGCGCCAAAGATCATCTTTTGCAAGctcttt                                                                                                                                                                                                               <  2:146983/238‑6 (MQ=255)                                                 tgACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGAAGCGGAAACGCTGTTTTATCCAGTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGAGAGGGATTTAAACCGGTCAGGTCTGGCACAGTGCTTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAATGCACCACATATCATCTTAGGAACGCACTTTATTGTGATTAGCGTc                                                                                                                                                                                                >  2:357736/1‑251 (MQ=255)                                                    ccGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCggg                                                                                                                                                                                             >  2:577543/1‑251 (MQ=255)                                                    ccGAGCTTTATACGGTGAAGGTAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTGACCTGAGGCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGTGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCATTTATTGTGATTAGCGTCggg                                                                                                                                                                                             <  2:294568/251‑1 (MQ=255)                                                      gAGCTTTATTCGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAAATAAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATTCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGa                                                                                                                                                                                           <  2:485521/251‑1 (MQ=255)                                                                 ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCggcg                                                                                                                                                                                               <  2:82922/236‑4 (MQ=255)                                                                 ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCg                                                                                                                                                                                               >  1:82922/1‑236 (MQ=255)                                                                     cAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATtgg                                                                                                                                                                            >  2:282900/1‑251 (MQ=255)                                                                                 aaaaCCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAgc                                                                                                                                                                >  1:18843/1‑251 (MQ=255)                                                                                                  aCGAAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaacggaa                                                                                                                                               <  2:201852/251‑1 (MQ=255)                                                                                                    gAAGTTTTATCCATTTTTGGTTAACTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGCTAGGGATTTATACCGGTCAGGTCTGGCAAAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGACAAATATTATCTTAGGCAAGCCCTATATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGAGCCTGAAGTTaacggcacg                                                                                                                                             >  1:162306/1‑251 (MQ=255)                                                                                                     atgTTTTATCTATTTGTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCTCTTTATTGTGATGTGCTTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaatggaacgg                                                                                                                                            <  2:102153/249‑1 (MQ=255)                                                                                                                      ggTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAccc                                                                                                                           >  1:153704/1‑251 (MQ=255)                                                                                                                      ggTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAccc                                                                                                                           <  1:563015/251‑1 (MQ=255)                                                                                                                      ggTTACCTGAGGCATTGATGGTTGATGGCAAGCAGAACGTGGAAGAACGCAATAGAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGTTGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGGTAACGGAACGGCGCTGATCATTGAAcc                                                                                                                            <  2:164429/250‑1 (MQ=255)                                                                                                                          aCCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGc                                                                                                                        <  1:631852/250‑1 (MQ=255)                                                                                                                                gTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGt                                                                                                                 >  1:404862/1‑251 (MQ=255)                                                                                                                                                ggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGGACCCGGCACCGGGTTAGAGCAAGGCGGGc                                                                                                  >  2:112640/1‑250 (MQ=255)                                                                                                                                                    aTCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGc                                                                                             <  1:282900/251‑1 (MQ=255)                                                                                                                                                       aGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTg                                                                                          <  1:600655/251‑1 (MQ=255)                                                                                                                                                                                 aTTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCTTGGTGTGGTGAAAGCGCCTGAAGTTACAGGAACGGCGCTGACCATTGAACCCGGCCACGGGGTAGAGCAAGGCGGGCAAGGCGTGCATAACCCTTTACCCGCAGGGGGCTg                                                                >  2:379258/1‑251 (MQ=255)                                                                                                                                                                                    tataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGc                                                              <  1:180003/250‑1 (MQ=255)                                                                                                                                                                                       aCCGGTCAGGTCTGGCACAGTTATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGAGAGCTCAACGCGCAAAATATCATCTTAGGCAAGCCCTTTATTGAGATTAGCGTCGGGGAGGCGCGGGGTATTGGTGGGGTGAAAGCGCCTGAAGTCAACGTAAAGGCGCTGACCATTGAACACGGCCCCGGGTTAGAGCAAGGCGGGGCGGGGGGGCATATCCCTTCACCCGAAaggggcatgagggc                                                          >  2:4996/1‑241 (MQ=255)                                                                                                                                                                                          ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                                                          <  2:291215/152‑1 (MQ=255)                                                                                                                                                                                          ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                                                          >  1:291215/1‑152 (MQ=255)                                                                                                                                                                                          ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGGAGc                                                        >  1:14395/1‑250 (MQ=255)                                                                                                                                                                                              aGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAAc                                                   <  1:380662/251‑1 (MQ=255)                                                                                                                                                                                                    ggCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGATAACGGAACGGCGCTGACCATTGAACCCGGCCCCGGGGTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGGCTGGCGGAAGCAAAACCTg                                                >  2:219591/1‑248 (MQ=255)                                                                                                                                                                                                         aGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCgg                                                                                                                         >  1:429680/1‑170 (MQ=255)                                                                                                                                                                                                         aGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCgg                                                                                                                         <  2:429680/170‑1 (MQ=255)                                                                                                                                                                                                             aTTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCGGGGCGTGCATATCCCTTTACCCGCAGGGGACTGGCgggagaggaaacttaaccgtaatatg                                    >  2:477872/1‑227 (MQ=255)                                                                                                                                                                                                                  acgctAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCt                               <  2:406772/247‑1 (MQ=255)                                                                                                                                                                                                                         aaGCCTATTGCGATGGTACGCGTCTGAGCGAGCTCAACGCTCCAAATTTCATTTGAGGCAAGCCCTTGATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATAGCCCGTGAACCGAAGGTGACTGGCGGAAGCAGAACCTGAAGAGGAATATGGCCCTGAATTTa                        <  2:673996/251‑1 (MQ=255)                                                                                                                                                                                                                         aaGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAACGTCGGGGAAGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAAAGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATAACCCTTTACCCGAAGGGGACTGGCGGCAGAAGAACAGGAAGCTGAATATGGCCATGGAttt                         >  1:415980/1‑250 (MQ=255)                                                                                                                                                                                                                            ccGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGc                     <  1:453262/251‑1 (MQ=255)                                                                                                                                                                                                                                 ttccaagTTTCGCCTCTTTGCGAGCTCAACTCGCCAAATATCTTCTTAGGCAAGCCCTTTATTTTGATTAGCGTCGGTGATGCGCGTGGTATTGGTGTGGTGAAAGCTCCTGAAGTTAACGGCACGGCGCGGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCTTGCATATCCCTTTACCCGAAGGTGACTGGCGGAAGCATATCCGGAAGCTGAATAGGGCCCTGAATTTAAGCGGTa                 <  2:335489/244‑1 (MQ=255)                                                                                                                                                                                                                                    gATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGTGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCgg             <  1:4996/251‑1 (MQ=255)                                                                                                                                                                                                                                             cgTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGACGAAGAACCTGCAGCTGAATATGGCCCTGAATTTTAGCGGGACCGGCGAttcttt    >  1:384571/1‑246 (MQ=255)                                                                                                                                                                                                                                             cgTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCGTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTc    <  1:647741/251‑1 (MQ=255)                                                                                                                                                                                                                                                  tAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTTGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtg  <  1:369662/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  GTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTG  >  NC_000913/4638320‑4638808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑