Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,354	C→T	L59L (CTC→CTT)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,354	0	C	T	100.0%	199.5 / NA	60	L59L (CTC→CTT)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/36);  total (24/36)

AGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGG  >  NC_000913/4638119‑4638593                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 aGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCtgtg                                                                                                                                                                                                                                   <  1:598509/251‑1 (MQ=255)       aaaTTCTTCCCACATATCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATTTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAATCTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAg                                                                                                                                                                                                                             <  2:418704/251‑1 (MQ=255)           tcttcCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCtt                                                                                                                                                                                                                          <  1:504562/250‑1 (MQ=255)                    aTAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGc                                                                                                                                                                                                                >  1:164429/1‑251 (MQ=255)                    aTAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTGGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGTAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGc                                                                                                                                                                                                                <  1:84071/251‑1 (MQ=255)                                 ccTGCTGACATTGCAAAGGAGAAGTCTATGTTGAAATCCTCCCTGTTCTGGTACATGTCCCCCTTGTTTGGTGCAGTATTGCTGTTGTTTATTCCGATAAAGCTGACTCGGCAAGGGATTGTCGAACGTGGCGATTATCTTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATTCGGTGTAGGAAGAGGATa                                                                                                                                                                                                    <  2:524426/250‑1 (MQ=255)                                       gcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAAcc                                                                                                                                                                                               <  1:315728/249‑1 (MQ=255)                                          atagCAAAGGAGAATATTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTTGTGCAGTATTGCTGTTGGTGATTCCGATAATGTTGATTCGGCAAGTGATTGGCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAATCCGTGGa                                                                                                                                                                                          <  2:123975/248‑1 (MQ=255)                                                  ggAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAAc                                                                                                                                                                                  >  1:420196/1‑251 (MQ=255)                                                  ggAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAAc                                                                                                                                                                                  <  1:45422/251‑1 (MQ=255)                                                    agaagaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAACACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAACCCGTGGAg                                                                                                                                                                                         >  2:58300/1‑242 (MQ=255)                                                    agaagaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAg                                                                                                                                                                                         <  1:58300/242‑1 (MQ=255)                                                      aagaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑a                                                                                                                                                                               >  1:232798/1‑249 (MQ=255)                                                       agaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGt                                                                                                                                                                            <  1:220653/251‑1 (MQ=255)                                                                    aTCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCAttttt                                                                                                                                                               <  1:527712/251‑1 (MQ=255)                                                                         cccgtttcgggaaaatgaccccattGTTTGGTGTAGTATTGATGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGATCGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGTGGGCCGCAAAAACTCGTTGGTCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATTCATTTTTGGTTa                                                                                                                                                          <  2:535481/227‑1 (MQ=255)                                                                            tGTTCTGGAAAATGAACACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCt                                                                                                                                                       >  1:352183/1‑251 (MQ=255)                                                                             gTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTg                                                                                                                                                      >  1:536789/1‑251 (MQ=255)                                                                                   gAAAATGACCACCTTGTTTGGAGCAGAATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCAAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGGGGAGCGGAAACG‑AGGTTTTATCCATTATTGGTTACCTggggcat                                                                                                                                                >  2:555033/1‑245 (MQ=255)                                                                                    aaaaTGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑ACGTTTTATCCATTTTTGGTTACCTGAGTCAtt                                                                                                                                               >  1:673996/1‑251 (MQ=255)                                                                                                       gTGCAGTTTTGCTGTCGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGGGCCGATGACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACGCGTTGGGACGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGGTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAg                                                                                                                            <  2:181873/251‑1 (MQ=255)                                                                                                       gTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCACGTGATAGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGAGTCGCCAAAGTACCATCGGGCCGCAAAAACTCGATGTGCCACTTCTCGCTATTACTGTGACCGAGCCTTATACGGTGCAGGAAGAGGATAAAACCGAGGCGCGGAC‑CGTAAGTTTTAACCATTCTTGGTTACCTGAGTCATTGATGGTTGCTGGCCATCa                                                                                                                             >  2:191940/1‑250 (MQ=255)                                                                                                                  cTGTTGTTGATTCCGATAATGATGATTCGGCAAGTGATTGTCGAACGTGCCGCTTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTAAGTCATTGACGGTTGATAGAAATCAGAACGTGga                                                                                                                    >  2:453262/1‑248 (MQ=255)                                                                                                                        ttgATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTTCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGaa                                                                                                                                                                                    <  2:34448/179‑1 (MQ=255)                                                                                                                        ttgATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGaa                                                                                                                                                                                    >  1:34448/1‑179 (MQ=255)                                                                                                                            ttCCGATAATGCTGATTCGGCCAGTGATTGTCGAACGTGCCGATCACCGTAGCGATGTGGAAGATGCGCTTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGATTATCGATATTCCTGTGACCGCGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AACTTTTATCCCTTTTTGGTTACCTGCGACATTGGTGGTTGATGGCATTCAGTACGTGGAAGAACGCAAGAt                                                                                                        >  2:38523/1‑250 (MQ=255)                                                                                                                             tCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAACGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGCGCTTTATACGGTGCAGGAAGAGGATAAAAGCGTGGAGCGGAAACG‑AAGTTTTATCCATTTGTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGACGAACGCaggaa                                                                                                        >  2:563015/1‑245 (MQ=255)                                                                                                                                   aaTGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGACGAACGCAAGATAGGGAttt                                                                                                >  1:102153/1‑251 (MQ=255)                                                                                                                                   aaTGCTGATTCGGAAAGTGATTGTCGAACGTGCCGAGTACCGTAGAGATGGGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGAttt                                                                                                <  2:547763/251‑1 (MQ=255)                                                                                                                                    atgctgagtgtgcaagTGATTGTCGACTGTGCCGAGTTCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACGCGTTGGTCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTGTGGTTACCTGAGTCATTGATGGTTGATGGCAAGCAGATCGTGGAAGAACGCAAGATAGGGATTta                                                                                               <  2:386117/236‑1 (MQ=255)                                                                                                                                     tGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTtat                                                                                              >  1:201852/1‑251 (MQ=255)                                                                                                                                          aTTCGGCAAGTGATTGTCGAACGTGCAGATTACCGTAGCGATGTGGAAGATGCGATTCTCCAAAGTACCAGGGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGGGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGCTTTATTCCgg                                                                                         <  2:562475/251‑1 (MQ=255)                                                                                                                                                   gTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑CAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCCAGATAGGGTTTTATACCGGTCAGGTCTg                                                                                >  2:262900/1‑251 (MQ=255)                                                                                                                                                     gATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTgg                                                                               <  1:62712/250‑1 (MQ=255)                                                                                                                                                           cGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGt                                                                         <  1:644496/250‑1 (MQ=255)                                                                                                                                                                  gccgATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCGTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAAc                                                                 <  1:182666/248‑1 (MQ=255)                                                                                                                                                                   ccgATTACCGGAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAACGGGACGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTATATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCACTCAGACCGTGGAAGAAAGCAAGATAGGGATTTATACCGGTCAGGTCTGACACAGAGTTTTAACg                                                                >  2:440251/3‑251 (MQ=255)                                                                                                                                                                      aatACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTAGCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACgcta                                                             <  1:457148/249‑4 (MQ=255)                                                                                                                                                                         aCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTaaaa                                                          <  1:622637/251‑1 (MQ=255)                                                                                                                                                                           cGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACg                                                                                                              <  1:138417/197‑1 (MQ=255)                                                                                                                                                                           cGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACg                                                                                                              >  2:138417/1‑197 (MQ=255)                                                                                                                                                                                ggATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGAtt                                                   <  2:232798/250‑1 (MQ=255)                                                                                                                                                                                  aTGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTc                                                 <  1:637969/251‑1 (MQ=255)                                                                                                                                                                                   tgtgGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCg                                                >  1:406772/1‑251 (MQ=255)                                                                                                                                                                                      ggAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATg                                             <  1:184833/251‑1 (MQ=255)                                                                                                                                                                                       gcaGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGGTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTAGCCATTGTTGGTTACCTGAGTCATTGATGGTTGATGTCAATCAGAACGTGGAAGTACGGAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGAGTTAACGCTAAAATCcgatgtcgatgt                                            <  2:333249/249‑1 (MQ=255)                                                                                                                                                                                          gaggcgtttCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTc                                         <  2:420196/244‑1 (MQ=255)                                                                                                                                                                                             gcgattacccgaaGTACCAGCGGGCCGGAAAAACCCGTTGGGCCGCTTATCGCTATTTCTGTGAACGAGCTTTATTCTGTTCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACTCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTcgc                                       <  2:103262/239‑1 (MQ=255)                                                                                                                                                                                                     ctgacgTACCATCGGGCAGCAAACACTCGTGGTGCCGCTTATCGCGATTACTGTGACCTAGCTTTATACGGTGTAGGTAGAGGATAAAACCGTGGATCGGACACG‑AAGGTTTATCCATTTTTGGGTACCTGAGTCATTGATTGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGAGTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATGTCGATGTCGCGCGTCtga                                 <  2:577287/243‑3 (MQ=255)                                                                                                                                                                                                     ccaaacttacatcaggCCGCAAAAACTCGTTGGGCCGCGTATCGCTATTCCTGTTCCCGAGCTTTATACGGTGCAGTAATAGGATAAATCCGTTGAGCGGTAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCTATCAGAACGTGGAAGAACGCAAGTTATGGATTTATTCCGGTCAGGTCTGGCACAGTGATTTAACGCGAACAGCCGTTTTCGATGTTTCGCGTCTTAGCg                              <  2:352120/237‑1 (MQ=255)                                                                                                                                                                                                         aGTACCAGCGGGCAGCCAAAAATAGGTGGGCCGCTTATCGATATTACTGTGCCCGAGATTTATACGGTGCAGGAAGAGGATAAAACCGTGGCGCGGAAACG‑AAGATTAATCCATTATTGGTTACCTGAGTCATTGATGGGTGATGGCAATCAGAACGTGGAAGACAGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTGAACGCTAAAAGCAGAATTCGATGTTTCGCGTCTTAGCGagct                          >  2:386817/1‑248 (MQ=255)                                                                                                                                                                                                          gTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTc                         <  1:487096/251‑1 (MQ=255)                                                                                                                                                                                                          gTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTc                         >  1:485521/1‑251 (MQ=255)                                                                                                                                                                                                            aCCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCaa                       <  1:59453/251‑1 (MQ=255)                                                                                                                                                                                                                  gggCCGCAAAAACTCGTTAGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGCGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTAGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGCTTTAACGCTAAAAGCCAATTTCGATGTTTAGCGTCTTAGCGAGCTCACCGCGcc                 >  2:380662/1‑251 (MQ=255)                                                                                                                                                                                                                   ggCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGt                                            <  2:85220/223‑1 (MQ=255)                                                                                                                                                                                                                   ggCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGt                                            >  1:85220/1‑223 (MQ=255)                                                                                                                                                                                                                   ggCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCa                <  1:29485/251‑1 (MQ=255)                                                                                                                                                                                                                             acgcGTTGGGCCGCTTATCGCTATTCCTGTGTCCGAGCTTTAGTCGGTTCAGGAAGAGGATAAAACTGTGGAGCGGAAACG‑AAGTTTTATACTTTTTTGGTTACCTGAGTCATTGATTGTTGATGGCAATCAGAACGTGTAAGAACGCAATATAGGTATTTATAACTGTCAGGTCTGGACCAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAtatagcagct      <  2:29651/248‑7 (MQ=255)                                                                                                                                                                                                                                 tttGGGCCGCTTATCGCTATTCCTGTGACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGTGTTTTATACCGGTCAGGTCTGGCACAGGGATTTTACGCTAAAAGCCGCTTTCGATGTTTCGCGTCTTAGCGAGCACAACGCTACAAATATAATCTTAgg  >  2:132353/2‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 AGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACG‑AAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGG  >  NC_000913/4638119‑4638593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑