Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,498	A→G	Y154Y (TAT→TAC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,498	0	A	G	100.0%	159.6 / NA	51	Y154Y (TAT→TAC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/20);  total (31/20)

GACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCT  >  NC_000913/7261‑7740                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   gACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCa                                                                                                                                                                                                                                       <  1:265600/251‑1 (MQ=255)       gggAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGa                                                                                                                                                                                                                                 <  1:153053/251‑1 (MQ=255)       gggAAGTTGCCCGAGAGTCAATACGCAAATCCCCAGGCTAGTCAGTACCCAGAATAGCGCCACCAACCGGACAAACCCCTGCATGAGCCGGGCGACGCCAAGAAGACCGCGAGTGATCGCCAGCAAAGCAAAGTCAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCACTGATCGCGAAACGGCGTCCGCCTGAAGTACGCTGAAAAGTATGCCGTAGGGGTTGAGCAAAAAGa                                                                                                                                                                                                                                 <  2:55406/251‑1 (MQ=255)             ttGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCg                                                                                                                                                                                                                            >  1:450525/1‑250 (MQ=255)              tGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTAGCCTGAATTCCGCTGCAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGa                                                                                                                                                                                                                           >  1:628505/1‑250 (MQ=255)                                              ggTCAGTACCCAGATGATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                                                                                            <  1:349658/249‑1 (MQ=255)                                                      cccAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCat                                                                                                                                                                                   >  1:672594/1‑250 (MQ=255)                                                        cAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCctat                                                                                                                                                                                 >  2:603899/1‑250 (MQ=255)                                                          aattctCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAcc                                                                                                                                                                              <  2:191002/246‑1 (MQ=255)                                                              aTCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGc                                                                                                                                                                          >  1:629151/1‑251 (MQ=255)                                                                  ccATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATCTACCATGCCgtc                                                                                                                                                                      >  2:144357/1‑249 (MQ=255)                                                                     ccAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCg                                                                                                                                                                   <  1:591218/250‑1 (MQ=255)                                                                         cGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCAc                                                                                                                                                               >  1:544714/1‑251 (MQ=255)                                                                              cAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGGCCGCCACGAAAc                                                                                                                                                          >  1:21169/1‑251 (MQ=255)                                                                               aaacccctgcatggtccagtcGACGCCATGAAGACCGGGAGTAAACGCCAGCAGAGCAAAGACATCGAGAATAATACCGGTCCACATCGGGGGAAAATTAAAAGAAAAACTCAGGTCTCGCGCAACGGCGTTCGCCTGAATCCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATTCCATCCCGGTCCGCCATGAAACt                                                                                                                                                         <  2:676757/231‑1 (MQ=255)                                                                                  ccccTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGcc                                                                                                                                                      >  2:234640/2‑251 (MQ=255)                                                                                   cccTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGccc                                                                                                                                                     <  1:234640/251‑1 (MQ=255)                                                                                              cctggCGACGCCATGAAGCCCGGCATAGATCGCTAGCAGAGCAAAGATAGCGTGAAGAATTCCTGTCACCAGCGGGAGAACATTAAAAGAAAAACGCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCGGAAAATTATGCCGTAGTCGTTGAGCAAAAAGACGGCGAACAGACCGCCCATCCACCGCATCCCCAGCCCGCGCGCCATATACCATGCTGTTCCGCCTCGAAACTGTCCAtgggcagctc                                                                                                                                          <  2:14699/248‑10 (MQ=255)                                                                                               cGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACCGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACg                                                                                                                                         >  2:571211/1‑251 (MQ=255)                                                                                                              aGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCACCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAgctg                                                                                                                          <  1:148519/251‑4 (MQ=255)                                                                                                                          atcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGGCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCAGCACTTTCTTTATAAAGCTGTGCGAGGGAACa                                                                                                              <  1:76969/248‑1 (MQ=255)                                                                                                                            cgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACAt                                                                                                             <  1:563648/249‑1 (MQ=255)                                                                                                                             gCCAGCAGAGCAAAGACAGCGAGAATAATACCTATCACCAGCGGGGGAAAATCAACAGAAAAACTCAGGGCTCGCGCAACGGCGATCGCCTGAATTCCGCTGAAAATTATGACGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATACCCAGCCCGCGCGCCATATACCATGCCGTTCCGACACGCAACTGCCCATTGGCATACCGATTTTTATAAAGCTGTGCGAGGGAACATTc                                                                                                           >  2:676572/1‑251 (MQ=255)                                                                                                                               cagcagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCgg                                                                                                         >  1:274353/1‑251 (MQ=255)                                                                                                                                     agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGCCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCaaa                                                                                                     >  1:283527/4‑249 (MQ=255)                                                                                                                                       caaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACg                                                                                                   >  1:444787/2‑249 (MQ=255)                                                                                                                                        aaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTggcatc                                                                                                                                            <  1:234920/207‑6 (MQ=255)                                                                                                                                        aaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTggcatc                                                                                                                                            >  2:234920/1‑202 (MQ=255)                                                                                                                                        aaaGACAGCGAGAATAATAACTGTCAACAGCGGGGGAAAGTCAAAAGAAAAACTCAGGGATCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAAAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCAATTGGCATCACGTTATTTATAAAGCTGTGCGAGGGAACCTTCGGCAAACGAgg                                                                                                >  1:588138/1‑251 (MQ=255)                                                                                                                                              aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc                                                                                           >  1:675250/1‑250 (MQ=255)                                                                                                                                                  agaATAATACCTGTCAACAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGAGCAACGGCGGTCGCCTGAATTCCGCTGAAAATTATGCAGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCAGCCACGACACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGCACATTCGGAAAACGAGGTCGcactgcc                                                                                      >  2:468907/1‑245 (MQ=255)                                                                                                                                                     ataataCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCACGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGCGGTCGCCATTCCGa                                                                                    >  2:330703/1‑250 (MQ=255)                                                                                                                                                       aataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                  >  1:617306/5‑250 (MQ=255)                                                                                                                                                          accTGTCACCAGCGGGGGAAAATCAAAAGGAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAGTTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGATGAGCGAGGGAACATTCGGCAATCGAGGCCGCCATGCCGATAAAcg                                                                              >  2:46497/2‑251 (MQ=255)                                                                                                                                                             ggTCACCAGCGGGGGAAAAGCAAAAGAAAAACGCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCgg                                                                           <  1:86498/250‑1 (MQ=255)                                                                                                                                                               tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGc                                                                          >  1:140285/1‑250 (MQ=255)                                                                                                                                                               tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGGCGCCCTGCCGATAAACGCGGc                                                                          >  2:508498/1‑250 (MQ=255)                                                                                                                                                                       gggggAACATCAAACGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGCAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCCGGTCCGCCCCGCAACTGCCCCTGGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGACAAACGAGGACGCCCTGCCGATAAACGCGGCCACCcacac                                                                 >  2:154775/1‑250 (MQ=255)                                                                                                                                                                            aaactCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCCCGCAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGCAGCCCTGCAGATAAACGCGTAAACCCACATcc                                                               >  2:195973/5‑248 (MQ=255)                                                                                                                                                                                 cAAAAGAAAAACTCAGGGCACGCGAAACGGCGTTCGCTTGAAGTCCGCTGAAAATTATGCCGCAGGCGATGAGCAAAAAGACGGCGAAAAGAACCCCCATCCAGCGCACCCCCAGCCCGCGCGCCTTATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGAc                                                        <  2:58691/250‑1 (MQ=255)                                                                                                                                                                                  aaaaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGCGCAAAAGGACGGCGAACAGAACGCCCCTCCAGCGCATCACCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGACACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGTGGTCGCCATGCCGCTAAACGCAGCAACCCACATCCAGAAGACgg                                                      >  1:320716/1‑251 (MQ=255)                                                                                                                                                                                                       cccaccggcgtccgcctaaatcccGCTAAAAATTAGGCCGTGGGCGATGCGCAAAAAGCCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTaa                                 <  2:672594/229‑1 (MQ=255)                                                                                                                                                                                                         cAACGGCGTTCGCCTGAATTCCGATGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAAATCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGGGGACGCCATGCAGTTAAACGCGGAAACCCACATCCAGACGACTGCCACAGGTCCACccgcggcaaat                               >  2:657629/1‑246 (MQ=255)                                                                                                                                                                                                          aaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCACCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAttg                              <  1:334784/251‑3 (MQ=255)                                                                                                                                                                                                          aaCGGCGTTCGCCTGAATCCCGCGGAAAATTATGCCGTAGGCGATGACCAAAAAGACGGCGAACAGAACCCCCATCCAGCGCTCCCCCAGCCCGCGCGCCATATCCCAGCCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAttg                              <  1:263093/251‑3 (MQ=255)                                                                                                                                                                                                                gatCGCCTGAATTCCGCTGAAAATTATGCCGAAGGCGATGCGCAAAAAGACGGCGAACAGCACGCCCAACCGGCGCATCCCCAGCCCGCGCGCCATATACCATGACGGGCCGCCACGAAACTTCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTAGGCAACAGAAGTAGCCCTGCCGAACAACGCGACAACACACATACAGAAGCAGGCTCCACATCCACcgccgggaaaatacagc                         >  2:376219/3‑239 (MQ=255)                                                                                                                                                                                                                     cctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTTCCCGCGCAAcc                   >  1:382952/3‑250 (MQ=255)                                                                                                                                                                                                                     cctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAAtttcccacgcaacc                   >  1:632394/3‑238 (MQ=255)                                                                                                                                                                                                                        gaattCCGCCGAAAATTATTCCGTCGGCGATGATCAAAAAGCCGGCGAACAGAACCCCCATCCAGCTCATCCCCAGCCCGCGCGCCATATCCCAAGCCGGGCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCTAGCTCAACGCCg                <  1:166098/247‑1 (MQ=255)                                                                                                                                                                                                                                     aaTTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCGc   >  1:67150/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTTATGCCGTAGGCAATGAGAAAAAAGCCGGCGAACAGACCGCCCAGCCAGCGCATCCCCAACCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCAGTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGACGCCCTGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCt  <  1:46497/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   GACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCT  >  NC_000913/7261‑7740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑