| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,637,976 | T→G | P427P (CCT→CCG) | creC → | sensory histidine kinase CreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,637,976 | 0 | T | G | 98.6% | 224.7 / ‑6.4 | 73 | P427P (CCT→CCG) | creC | sensory histidine kinase CreC |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (42/30); total (42/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑ACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGT > NC_000913/4637732‑4638217 | cAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGc > 1:535481/1‑250 (MQ=255)cAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGATGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGAC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGGATTCCTGCCTACGCGCTGTCACGTCTTGTTGAACGCTTTTACTCTTTGCCGCGTGc > 2:598509/1‑250 (MQ=255) aGAAAAAAACATCACTCTACATGTTATGCACACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGCTCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGAGGTATTCCTGACTACGCGCTGTCCCGTATTTTTGAACGCTTTTAATCTTTGCCGCGTGcc > 1:433202/1‑249 (MQ=255) aaaaaaacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAt < 1:628074/251‑1 (MQ=255) aaacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGc > 1:281782/5‑251 (MQ=255) tCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaaaa > 1:547763/1‑250 (MQ=255) ctctACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTCGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAAAAAagc > 1:655592/1‑251 (MQ=255) ctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagc > 2:161893/4‑249 (MQ=255) gTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCt > 1:263633/1‑251 (MQ=255) gCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGt > 1:562475/1‑251 (MQ=255) tgAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGCAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGCTACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTATTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGTCGt > 2:409127/2‑251 (MQ=255) aGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTc < 2:281782/251‑1 (MQ=255) aGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTc < 1:3515/251‑1 (MQ=255) tgTTGCTGCTGAACCCGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGCTTTTACCACCGAGAGCGGTGGCATAACGCTAAGCGACGAAGTGGCTCAGGGACACGTC‑GCCCTTAAGGTGCTGGCTACCGGTAGTGGAATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGGGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGTGTTAGTCAGTg > 2:264863/2‑251 (MQ=255) tgctgcTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAgg > 1:181873/1‑251 (MQ=255) gctgctGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATTCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTTAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGt < 1:264863/251‑1 (MQ=255) gctgctGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGGAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCAGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTccttggtggagg > 2:587711/1‑240 (MQ=255) ctgAACCGGCGTTACTGGAGCCGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTTCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGGTGGCGTTCGTCAGTGAGGTCGcc > 2:121530/1‑251 (MQ=255) gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCg < 1:428969/251‑1 (MQ=255) aCCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCgtt < 1:161893/251‑1 (MQ=255) cGGCGTTACTGGAGCAGACGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGCGCGGTCGCATAACGCTAAGCGCCGCAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGAGGTATTCCTGACTACGCGCTGTCCAGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAACTGGGCAAAATAGCAGCGGCCTGGGGTTGGCGTTCGTCAGTggtgtcgcacggctg > 2:644496/1‑237 (MQ=255) ggCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCgtttgt < 1:7121/251‑1 (MQ=255) gCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGGCGCCCGTTTGTTTAACGGCGa > 2:62712/1‑249 (MQ=255) aaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCa > 1:524426/1‑250 (MQ=255) tttACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAACTGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGACGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACg > 1:56344/1‑251 (MQ=255) ttACTGGATATCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGTACACGGC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGGCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTGCGTCAGTGAGGTCGCCCGTTTGTTTAACGTCGAAGTCACGCTGCGCAACg < 2:655592/250‑1 (MQ=255) tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt < 1:184287/250‑1 (MQ=255) tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGCCGCATAACGCTAAGCGACGAAGTGGATCAGGAACACGTC‑GCCCTTATGGAGCTGGATACCGGTAGTGGTATTCCTGACTACGCGATGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGAGAGGCCGCCCGATTGTTTAACGGAGAAGTCACGCAGCGCaaaggg > 2:622637/1‑247 (MQ=255) ggATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAgg < 1:459385/251‑1 (MQ=255) gATAACGCCATCGATTTTACCCCCGAGAGCGGACGCATAACGCTAAGCGCCGAAGTGGATCAGGAACCCGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCCCGTATTTTTGAACGCTTTTACTCTTTGCCGCGGGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGTTTAACGGCGAATACACGCTGCGCTACGTGCAgg > 2:329964/1‑250 (MQ=255) taactacATCGATTTTACACCCGAGAGCGGCCGCATAACGCTAAGCGCCGAAATTGATCAGGACCACGTCGAC‑CTTAAGGTGCTGGTTACCGGAAGTGGTATTCCTGACTAAGCGCTGACACGTATTTTTGAACGAATTTCCTCTTTGCCGCGTGCACATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGCCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTAACGCTGCGCAACGTGCAGGa > 2:586035/7‑249 (MQ=255) aTCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaaaaaa < 1:423594/163‑1 (MQ=255) aTCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaaaaaa > 2:423594/1‑163 (MQ=255) aCCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGCCTACGCGCTGTCACGTATTTTTGAAAGCGTTTACTCTTTGTCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGAGAGGGCGCCCGTTTTTTTAACGGCGAAGGCACGCGGCGCGCAGTGCAGGAAGGGGGCGTGCTGGGCTc > 2:558172/1‑251 (MQ=255) cccccGATAGCGGTCGCATAAAGCTAAGCGCCGAAGTGAATCAGGCACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCATGACTACGCGCTGTCACGTATTTATGAACGCTTTTACTCTTTTCCGCGTGCAAATTGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAAGGAGGATCCACGCTGCGAAACGTACAGGAAGGGGGCGTgatgtccgcg > 2:182666/1‑242 (MQ=255) cccccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCATGCTGGCCTCg > 1:352120/1‑251 (MQ=255) cGGTCGCATAACGCTAAGCGCCGAAGTGGATCCGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAg > 2:465871/1‑171 (MQ=255) cGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAg < 1:465871/171‑1 (MQ=255) cGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTc > 1:29651/1‑251 (MQ=255) cGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGAGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTACGTGAGGTCCCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGAGCAGGCCTCGCTTCGTCttt > 2:527712/1‑250 (MQ=255) aCGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCg < 1:405711/236‑1 (MQ=255) aCGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGGGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCg > 2:405711/1‑236 (MQ=255) tAAGCGCCGAAGTGGATCAGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCACATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCACCGTGCAGGAGGATGGCGTGCTGGCCTCGCTTCGACTTCACCGTCCCTTccc > 2:220653/1‑248 (MQ=255) cGAAGTGGATCAGGAACACGTC‑ACGCGTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCACGAAGGTGGCGTGCTTGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTc < 1:69898/251‑1 (MQ=255) aGGAACACGTC‑GCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTccc < 1:14315/251‑1 (MQ=255) gtgacacGTC‑GCCCTTAAGGTGCTGTATACCGGTAGTGGTATTCCTGATTACGCGCTGTCACGTATTTGTGAACGCTTTTTCGCTTTGCCGCGTGGAAATGGGCAAAAAAGCATCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGGGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCca < 2:545939/248‑1 (MQ=255) acgtcgcccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGAGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGAGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCAACAt > 2:487096/9‑249 (MQ=255) cgtcgcccTTAAGGTGCTGGATCCCGGTAGTGGGATTCCTGACTACGCGCTGTCACGCATTTTTGACCGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGGGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCCCATCGCTTCAAATTCTTCCCACATAgc > 2:576003/8‑250 (MQ=255) gtcgcccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGCGGCGGGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGATTCAAATTCTTCCCACATAgcc > 2:571366/7‑249 (MQ=255) cgcccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATaacctt > 2:239199/5‑246 (MQ=255) ccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGt > 1:418704/2‑251 (MQ=255) tAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCTCGTATTTTTGAACGCTTTTACTCGTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTGCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCTGCACTTCACATAGCTGCAAATTCTTCCCACATAGCCGTCGTa < 1:225508/249‑1 (MQ=255) aaGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATc < 1:86737/250‑1 (MQ=255) aggttccggATACCGGTAGTGTGATTCCTGACTACGCGCTGTCACGTAGTTTTGAACGTTTTTACTCTTGGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTTCTGTCCTCGCTTCGACTTCACCTTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCTTATCct < 2:119926/244‑1 (MQ=255) ggATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGcct > 1:183997/1‑250 (MQ=255) aTACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCAtt < 1:239199/251‑1 (MQ=255) aGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGga < 1:571366/251‑1 (MQ=255) tACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGCGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCcaca > 2:183393/1‑204 (MQ=255) tACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGGCACTTCACATAGCTTCAAATTCTTCCcaca < 1:183393/204‑1 (MQ=255) aCGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGaaa < 1:69019/251‑1 (MQ=255) cgcTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTTGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATcc < 1:31996/251‑1 (MQ=255) cgcTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATcc < 1:121530/251‑1 (MQ=255) gtCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATccccc < 1:576003/249‑1 (MQ=255) gtCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATcccc < 1:409127/248‑1 (MQ=255) ggcACGTATGTTGGAGCGCTTGTAGTGTTTGCCGCTTGCAAATGGGCAAAAAAGCAGCAGTCTGGTGTTGGCGTTCGTCAGTGTGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTAACCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATcccccc < 2:137586/249‑1 (MQ=255) cACGTTTTTTTGAACGCTTTTACTCTTGGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGTCGAAGTCTCGCTGCGCAACGTGCAGGAATGTGGCGTGCTGTCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAGTTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTg < 1:381460/251‑1 (MQ=255) aTTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctgc > 1:180544/1‑206 (MQ=255) ttttGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTgg > 1:335489/1‑250 (MQ=255) gCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCTGCGGTCTGGGGGTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGTCCTCGCTTCGACTTCTCCGTCACTTCACATATCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGAcc < 1:73460/251‑2 (MQ=255) ttttACTCTTTGCCGCGTGCAAATGGGCAAAACAGCAGCGGTCTGGGGTTGGCGTTCGTAAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTAACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCCTTGCAATGTAGAAGACTAGGTTGAAATCCCCCCTTTTATGGAAAAtaaccac > 2:29485/1‑245 (MQ=255) ctctTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTATGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGAccaccttgt > 1:577287/1‑243 (MQ=255) tctTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGt > 2:536709/1‑111 (MQ=255) tctTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGt < 1:536709/111‑1 (MQ=255) | CAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑ACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGT > NC_000913/4637732‑4638217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |