Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,697	C→T	F334F (TTC→TTT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,697	0	C	T	98.1%	163.7 / NA	53	F334F (TTC→TTT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/30);  minor base G (0/1);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACG  >  NC_000913/4637450‑4637938                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ggAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTtcgc                                                                                                                                                                                                                                                >  1:273418/1‑251 (MQ=255)             ttGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCgg                                                                                                                                                                                                                                     >  1:410181/1‑249 (MQ=255)               gAGCAGTATGTTTATGCCTTAACCCATGAGCTAAAAAGCCCCCTGGCGGCGATTCGTGGCGCTGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAgc                                                                                                                                                                                                                                  <  2:151128/251‑1 (MQ=255)                    gTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCa                                                                                                                                                                                                                             <  2:410181/251‑1 (MQ=255)                     tATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCAc                                                                                                                                                                                                                            >  1:119926/1‑251 (MQ=255)                      atgttgtggcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATGCGTGGCGCGGCGGAAATGTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTTGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGTCTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCTAAGCGCGCAcc                                                                                                                                                                                                                           <  2:200249/240‑1 (MQ=255)                        gtttatgcggtAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGt                                                                                                                                                                                                                         <  1:512153/241‑1 (MQ=255)                        gTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGCCAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCTGACTGTTGTTGTTGTGGCGGCCTTTTTTCGCCGCGTcctgcaacgcggcaccgg                                                                                                                                                                                                                         >  2:590671/1‑234 (MQ=255)                              gcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGtt                                                                                                                                                                                                                   <  1:264149/248‑1 (MQ=255)                                  tAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGc                                                                                                                                                                                                                <  1:536252/250‑1 (MQ=255)                                                aaaaGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCAc                                                                                                                                                                                                 <  1:68293/251‑1 (MQ=255)                                                 aaaGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCAct                                                                                                                                                                                                <  1:599127/251‑1 (MQ=255)                                                  aaGCCCACGTGCGGCGATTCGTGGCGCGGCGGAAAGTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTGTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGTCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAAGCGTCAGGAAGTCGTTCTGGCTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCActc                                                                                                                                                                                               <  2:130186/251‑1 (MQ=255)                                                       cACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACAt                                                                                                                                                                                          <  1:590671/251‑1 (MQ=255)                                                           ggcggcGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTa                                                                                                                                                                                      <  2:273418/251‑1 (MQ=255)                                                                               cggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCGGAAAAAAACATCACTCTACATGttttgccccctgggggtcatgg                                                                                                                                                                   >  2:483413/1‑230 (MQ=255)                                                                               cggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTaatgt                                                                                                                                                                   <  1:483413/250‑4 (MQ=255)                                                                                          cgcgAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAAcc                                                                                                                                                       <  1:546005/251‑1 (MQ=255)                                                                                                    cgccaccgGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTg                                                                                                                                             >  1:604728/1‑251 (MQ=255)                                                                                                           ggAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTAATTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCActg                                                                                                                                                                             <  1:383312/212‑3 (MQ=255)                                                                                                                        cGTTTTCCCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATGGGTAGAACCGTTACTACGCCAGGCAAGAC‑GGGGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTa                                                                                                                        <  1:366674/251‑1 (MQ=255)                                                                                                                        cGTTTTACCGTCAACATTCTGACGCAAAATGCGTGTATGCATGCAGTGGTCGAAGCGTGACTACGCCAGGAAAGACGGGAGAATCGGCGGGAAGTCGATCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCTTCAGCGAAGCGCTCACCTTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAttt                                                                                                                         <  2:496484/251‑1 (MQ=255)                                                                                                                        cGTTTTACCGACAACATTCTGACGCAAAATGCGCGTCTGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGAACCGGGAAGTTGGCAGGAAAAAAAATCACTATACATGTTATGCCCCCTGAGGTTCATGTTGCTGCTGATACGTCGGTTCTGGAGCAGGCGCTgggggattt                                                                                                                         >  2:221255/1‑245 (MQ=255)                                                                                                                                      caTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTGACTCGAGCAGGCTCTGGGGAATTTACTGGCTAACGCCa                                                                                                           <  1:221255/251‑1 (MQ=255)                                                                                                                                           tGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGAt                                                                                                      >  1:165030/1‑251 (MQ=255)                                                                                                                                             aCGCAAAATGCGCGTATGCAGGCATTGGGAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGACGCGTAAGCGAAGCCCGCACCGTTCAGTTGGCAGAAAAAAACATCACTCTACATGTTGTGCCCCCTGAGGTTAATGTTGCTGCTGGACAGGCGTTAATGGAGCAGGCGCTGGGGAATTTTCTGGCTAATGCCATCGtttt                                                                                                    >  2:62811/1‑251 (MQ=255)                                                                                                                                               gCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTa                                                                                                  <  1:621564/251‑1 (MQ=255)                                                                                                                                                      gcgcGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCg                                                                                            <  1:331274/250‑1 (MQ=255)                                                                                                                                                            aTGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGc                                                                                                                                                                                                                <  1:305675/128‑1 (MQ=255)                                                                                                                                                            aTGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGc                                                                                                                                                                                                                >  2:305675/1‑128 (MQ=255)                                                                                                                                                               cAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGTCTGGAGAATCGTCAGGATGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATTTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTGCCCCCGAGAGctgtcg                                                                                  <  2:411026/251‑6 (MQ=255)                                                                                                                                                               cAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGtcg                                                                                  <  1:146076/251‑3 (MQ=255)                                                                                                                                                                       ggTAGAAACGTTACGACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACg                                                                           <  1:77041/250‑1 (MQ=255)                                                                                                                                                                          aGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCTGACTGATGTTGATGTGGCGGAGTTATTTCGCCGCGTCAGCGAAACGCGCACCGTGCAGTTGGCAGAAAAAACCATCACTCTACATGTTATGCCCCCTGAGGTTACTGTGGCTGCTGAACCGGCGTTACTGGAGGAGGCGCTTGGGAATTTACTTGATAACGACATCGATTTTACCCCCGAGATCGGTCGCATAACGcgat                                                                       >  2:314724/1‑248 (MQ=255)                                                                                                                                                                                gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGACGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGATGGCAGAAAAAAAAATCACTCTTCATGTTATGCCCACTGGGGTTACTGTTGCTGATAAACCGGAGGTACTGGAGCAGACGGTGGGGGATTTACTGGATTACGCCATCGGATTTACCCCCCGGAGCGGtccgataaccctaagggccg                                                                 >  2:184287/1‑232 (MQ=255)                                                                                                                                                                                  tactacGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGa                                                                <  1:178703/250‑1 (MQ=255)                                                                                                                                                                                  tactacGCCAGGCAAGACTGGAGAATCGTCAGGAAGCCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGGGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCCTCGATTTTACCCCCGCGAGCGGTCGCATAACCCTAAGCGCCGa                                                                >  1:587465/1‑250 (MQ=255)                                                                                                                                                                                   actacGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATACCGCTAAGCGCCGa                                                                >  1:123975/1‑249 (MQ=255)                                                                                                                                                                                        gCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGAt                                                         <  1:352773/251‑1 (MQ=255)                                                                                                                                                                                              cAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTGTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCCCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGCACCGGCGGTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCTATTTTACCCCCGAGGGGGGTCGCATTATGCTAAGCGCCGAAGTggggcaggg                                                    >  2:459385/1‑243 (MQ=255)                                                                                                                                                                                                   cTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGCTCAGGAACACGt                                              >  1:186844/1‑251 (MQ=255)                                                                                                                                                                                                     ggAGAATCGTCATGAAGTCGTTCTGACTGTTGTTGATGTGGCTGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTcg                                            <  2:526041/251‑2 (MQ=255)                                                                                                                                                                                                     ggAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGt                                              <  1:306957/249‑1 (MQ=255)                                                                                                                                                                                                                   aaGTCGTTCTGACTGTTGTTGATGTGTCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTg                              <  1:306417/251‑1 (MQ=255)                                                                                                                                                                                                                     gTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTgg                             >  1:61088/1‑250 (MQ=255)                                                                                                                                                                                                                        gTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGCGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGCGAGCGGTCGCATAACGCTAAGCGCCGCAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATAc                         >  1:69701/1‑251 (MQ=255)                                                                                                                                                                                                                         ttCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGc                                                                                                             >  1:420173/1‑166 (MQ=255)                                                                                                                                                                                                                         ttCTGACTGTTGTTGATGCGGCGGCATTATTGCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACGCTACATGTTATGCCCACTGATGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGc                                                                                                             <  2:420173/166‑1 (MQ=255)                                                                                                                                                                                                                           cTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCTCCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCCAGAGCGGTCGCCTAACGCTAAGCGCCGAAATGGAGCAGGAACACGGCGCCCTTAAGGTGCTGGATAcccg                      >  1:430984/1‑249 (MQ=255)                                                                                                                                                                                                                             gactgtggTTGATGTGGCGGCATTATTTCTCCGCGTCAGCGAAGCGCGCACCGTGCCGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTACTGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGAAACCCGTCGCCCTTAAGGTGCTGGATACCGGt                     >  1:465482/8‑250 (MQ=255)                                                                                                                                                                                                                                  ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGGGCTGGATACCGGTAGTggg               >  1:137586/2‑250 (MQ=255)                                                                                                                                                                                                                                         ggtgGCTGCATTATTTCGCCGCGTCAGCGAAGCGCGAACCGTGCAGTTGGAAGAAAAAAACATCACTCTACATGTTATTCCCACTGAGTTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAGCGCCATCGATTTTCCCCCCGAGAGCGGTCGCATATCGCTAAGCGCCGAAGTGGATCATGAACACGTCGCCCTTAAGGGGCTGGATACCGGTAGTCGTATTCCt         <  2:604728/249‑1 (MQ=255)                                                                                                                                                                                                                                              cggcATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGACAGAAAAAAACATAACTCTACATGTTATGCCCACTGCGGATAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTAc   >  1:309899/1‑251 (MQ=255)                                                                                                                                                                                                                                               ggcATTATTTCGCCGCGTCAGCGGAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTAcg  <  1:489582/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  GGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACG  >  NC_000913/4637450‑4637938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑