Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,821	G→A	L42L (TTG→TTA)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,821	0	G	A	93.2%	115.4 / ‑2.4	44	L42L (TTG→TTA)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (24/17);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCG  >  NC_000913/4636578‑4637051                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       aCGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTTAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGGGGGGGCGTTAATCgca                                                                                                                                                                                                                                  >  2:256099/1‑248 (MQ=255)                   cccACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACGGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAGGAAGTTAAACCAGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCgg                                                                                                                                                                                                               >  1:47234/1‑251 (MQ=255)                                       gCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGAGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGCCTCGGCCTGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAGGTTAATCGATACCGCCACGTTTCGGGGAGAGCTGGGTCGTCCCGCTTTg                                                                                                                                                                                           >  1:279435/1‑251 (MQ=255)                                        ccAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGc                                                                                                                                                                                          >  1:233709/1‑251 (MQ=255)                                                  cgcCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACAGGGCGTGCGAAGAGCAACGGAGGGGACGTTAAACGATACCGCAACGTTGCTGGCGGAGCTGGCGCATCCCGCTTTGCTCTCTtgggg                                                                                                                                                                                >  1:397021/1‑250 (MQ=255)                                                         aaCCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACg                                                                                                                                                                         <  1:256099/251‑1 (MQ=255)                                                          aCCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGCTACCGCAACGTTGCTGGCGGAGATGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGc                                                                                                                                                                        >  1:446109/1‑251 (MQ=255)                                                               gATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGGACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGGCGTTAATCGCTAACGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCCTggg                                                                                                                                                                   >  1:64133/1‑251 (MQ=255)                                                                                    cATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAAt                                                                                                                                              <  1:596414/251‑1 (MQ=255)                                                                                          ggCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGCTTTGCTCTCTGGGGGCCCAACGCATGGGCAACTGGCGCAGGGGTTTAATCAGCt                                                                                                                                         >  1:529616/1‑250 (MQ=255)                                                                                                     taGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGGGGTGGCGCGTCCCGATTTGCTCTCTGGGGGGCCAACGCATGGGCAACTGGCGCCGGGGGTTAATCAGCTACAACATCGccc                                                                                                                             >  1:97660/1‑251 (MQ=255)                                                                                                                       aTGCGTATCGGCGTGCGGTTGCTGCTGTGCTTGTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGTGTCGTTAATCGATACCGCTACGTTGCTGGCGGAGCTTGCGCGTCCCGATTTGCTCTCTGGGTTCCCAACGCAGGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCgg                                                                                                           <  2:469456/251‑1 (MQ=255)                                                                                                                           gTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGc                                                                                                       <  1:36553/251‑1 (MQ=255)                                                                                                                                  cATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACa                                                                                                <  1:675297/251‑1 (MQ=255)                                                                                                                                        gttgctGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACCCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACCTCGCCCGTTTCGCGCCAATATCGGGGGCATTTACAAAATgc                                                                                          >  1:291978/1‑251 (MQ=255)                                                                                                                                         ttgctgCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGACGTTAAACCGGGCGTGCGAAGAGCAAAGGAGGGGACGTTAAACGATACCGCAACGTTGCTGGCGGAGCTGGCGCGGCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCCGATCAAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACCAAGTgcg                                                                                         >  1:602867/5‑251 (MQ=255)                                                                                                                                         ttgctgCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGGTGCATTAACAAAGTgcg                                                                                         >  1:596264/5‑251 (MQ=255)                                                                                                                                             tgCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAAAAAAGTGCGCAAt                                                                                     >  1:656271/1‑251 (MQ=255)                                                                                                                                              gCTGGGTTATTTTTTACGGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTGCGCGCCAATATCGGTTGCATTAACAAAGTGCGCAATg                                                                                    <  2:446109/251‑1 (MQ=255)                                                                                                                                                 gggCTTTTTTTTACGTGTGGCGGGGGCGGCCTGGTTCGTACTGGCAATTTTTGGCAAAGAAGTTAATCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGTGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGTCGTAGGCGTTTAATCAGCTACAACATCGCCCGTTGCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGaa                                                                                  <  2:379891/250‑1 (MQ=255)                                                                                                                                                 gggCTATTTTTTACTGGGGACGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGGCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATTCCGCAACGTTGCTGGCTGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGaa                                                                                  <  1:60120/250‑1 (MQ=255)                                                                                                                                                 gggCGATTTGTTACTGGTGGCGGTGGGAGGCTGGTTCGTACTGGCCATTTTTGTCAAGGAAGGTAATCCGGGCGTGCGAAGAGCAACGGAGGGGACGGTGATCGACACCGCGACGTTGCTGGCGGAGCTGGCGCGTACCGAGTTGCTCTCTGGGGACCCAACGCATGGGCAACGGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTGCGCGCCAATATCGGTGGGATTAACAAAGTGCGCAATGaag                                                                                 <  2:47234/251‑2 (MQ=255)                                                                                                                                                  ggCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGGACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAata                                                                                <  1:482146/251‑1 (MQ=255)                                                                                                                                                      aTTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGTGGCATTAACAAAGTGCGCAATGAATATCAt                                                                            >  1:255465/1‑251 (MQ=255)                                                                                                                                                        tttttACTGGTGGCGGTGGCGGCCTGGTTCGTACTGTCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTa                                                                                                                                                <  2:379879/181‑1 (MQ=255)                                                                                                                                                        tttttACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTa                                                                                                                                                >  1:379879/1‑181 (MQ=255)                                                                                                                                                                gtgggcggtggcGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTTGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTatat                                                                    <  1:275639/246‑1 (MQ=255)                                                                                                                                                                   ggcggtggcGGCCTGGATCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTACCGATTTGCTCTCTGGGGACCCAACGCAGTGGCAAACGGCGCCGGCGTTTAATCAGCTACAAACTTGCCCCTTTCGCGCCAAAATCGGTGGCATTAACAAAAGGGGCCATGACTATCATGTCTTAATGACCg                                                               >  2:374813/1‑251 (MQ=255)                                                                                                                                                                    gcggtggcGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAATTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGa                                                              <  1:492521/251‑1 (MQ=255)                                                                                                                                                                       gtggcgtccTGGTTGGTACTGGGTATTTGTGTGATAGAAGTTAAACCGGTGGTTCGAAGAGCAACGGAGTGGCCTTTAAGGGATACCGCAACGTTTCTGGCGTAGCTTGCGCGGCCCGATTTGCGCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCGCGTTTCGCGCCAAGATCGTTGGTATTAACAAAGTGCGCAAGGAATGTCATGTCTATATGCCCggttc                                                           <  2:111256/244‑5 (MQ=255)                                                                                                                                                                            ggCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACGGGGAGTGCGAAGAGAAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGGTTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAACGGCGCAATGAATATCATGTCTATATGACCGATTCGCAgg                                                      >  1:565799/2‑251 (MQ=255)                                                                                                                                                                              ccTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAgggt                                                    <  1:20422/251‑2 (MQ=255)                                                                                                                                                                               cTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAgggt                                                    >  1:183773/1‑249 (MQ=255)                                                                                                                                                                               cTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGGTGCTGGCGGAGCTGGCGCGGCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGGTTAATCAGCTACAACATCGACCGTTTCCCGCCAATAACGGTGGCATTAACAACATCCGCAATGAATATCATgacaataaaaccgattcgccgggt                                                    >  2:134192/1‑227 (MQ=255)                                                                                                                                                                                       tACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCCACTGGCGCCGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCCATGAAAATCATGTCTCTATGACCGATTCGCAGGGTAAAGTATTg                                           >  2:229325/1‑251 (MQ=255)                                                                                                                                                                                               aTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGAtt                                    <  1:374813/250‑1 (MQ=255)                                                                                                                                                                                               aTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAAGTTAATCGATACCGCAACGTTGCTGGCGGCGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGAAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGGGGCATTAACAAAGTGCGGAATGAATATCAAGTCTATATGACCGATTCGCCGGGTAAAGAATTGTTCGATTc                                   >  1:565927/1‑251 (MQ=255)                                                                                                                                                                                                gtgtgttCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAATGGAGGGGACGGTAATCGATACCTGAACGTTGCTTGCGGAGCTGGCGCGGCCCGATTTTCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCATCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGGGCGCAATGAATATCATGTCTATATGACCGTTTCGCAGGGTAAAGTATTGGTCGATTc                                   <  2:333231/244‑1 (MQ=255)                                                                                                                                                                                                       cAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAAt                            >  1:489152/1‑250 (MQ=255)                                                                                                                                                                                                        aaaGAAGTTAAACCGGGCGGGCGAAGATCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATaa                          <  2:480453/251‑1 (MQ=255)                                                                                                                                                                                                         aagaagTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATaa                          >  1:126663/1‑250 (MQ=255)                                                                                                                                                                                                                tAAACCGGGCGTGCGAAGAGCATCGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCgg                                                                                                           <  2:266902/162‑1 (MQ=255)                                                                                                                                                                                                                tAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCgg                                                                                                           >  1:266902/1‑162 (MQ=255)                                                                                                                                                                                                                                agagCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTAGCAGGGTAAAGTATTGTTCGATTAGTCAAATAAAGCCGTTGGGCAGGATTATTggcg  >  2:572861/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       ACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCG  >  NC_000913/4636578‑4637051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑