Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,767	C→T	A24A (GCC→GCT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,767	0	C	T	93.0%	108.9 / 0.8	44	A24A (GCC→GCT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (23/17);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

GTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTG  >  NC_000913/4636523‑4637010                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    gTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttttt                                                                                                                                                                                                                                               >  1:480453/1‑251 (MQ=255)     ggTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGt                                                                                                                                                                                                                                             <  1:71973/249‑1 (MQ=255)                    taaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCTGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGCGGCGGGCTGGTTCGTACTGGCTATTTTTGTCAAATAAGGTAAAca                                                                                                                                                                                                                              >  1:423201/3‑248 (MQ=255)                             gCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGaa                                                                                                                                                                                                                   <  1:665260/251‑1 (MQ=255)                                   gggAAGATGCGCAGGAAACCTACGATCGCACCGTTGATACCCACATTAAAACGTGGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCaa                                                                                                                                                                                                             <  2:542961/251‑1 (MQ=255)                                     gcaGATGCGCAGCACACCTACGATCGCACCGTTGATACCCACATTAAAACGATGCGTGCCAAGCTGAGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGACATGGAATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGATGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTATTGGGTATTTTTGACAAGGAAGATAAAACgggggagctgagaggaacg                                                                                                                                                                                                           >  2:580329/3‑235 (MQ=255)                                     gAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGTTTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACg                                                                                                                                                                                                           >  1:518576/1‑251 (MQ=255)                                     gAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCTGGCGTGCGAAGAGCAACg                                                                                                                                                                                                           <  1:178946/251‑1 (MQ=255)                                       aGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCGTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCGGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGa                                                                                                                                                                                                         <  2:438289/251‑1 (MQ=255)                                                        aCGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTTAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGGGGGGGCGTTAATCgca                                                                                                                                                                                         >  2:256099/1‑248 (MQ=255)                                                                          cccACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACGGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAGGAAGTTAAACCAGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCgg                                                                                                                                                                      >  1:47234/1‑251 (MQ=255)                                                                                              gCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGAGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGCCTCGGCCTGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAGGTTAATCGATACCGCCACGTTTCGGGGAGAGCTGGGTCGTCCCGCTTTg                                                                                                                                                  >  1:279435/1‑251 (MQ=255)                                                                                               ccAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGc                                                                                                                                                 >  1:233709/1‑251 (MQ=255)                                                                                                         cgcCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACAGGGCGTGCGAAGAGCAACGGAGGGGACGTTAAACGATACCGCAACGTTGCTGGCGGAGCTGGCGCATCCCGCTTTGCTCTCTtgggg                                                                                                                                       >  1:397021/1‑250 (MQ=255)                                                                                                                aaCCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACg                                                                                                                                <  1:256099/251‑1 (MQ=255)                                                                                                                 aCCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGCTACCGCAACGTTGCTGGCGGAGATGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGc                                                                                                                               >  1:446109/1‑251 (MQ=255)                                                                                                                      gATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGGACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGGCGTTAATCGCTAACGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCCTggg                                                                                                                          >  1:64133/1‑251 (MQ=255)                                                                                                                                           cATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAAt                                                                                                     <  1:596414/251‑1 (MQ=255)                                                                                                                                                 ggCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGCTTTGCTCTCTGGGGGCCCAACGCATGGGCAACTGGCGCAGGGGTTTAATCAGCt                                                                                                >  1:529616/1‑250 (MQ=255)                                                                                                                                                            taGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGGGGTGGCGCGTCCCGATTTGCTCTCTGGGGGGCCAACGCATGGGCAACTGGCGCCGGGGGTTAATCAGCTACAACATCGccc                                                                                    >  1:97660/1‑251 (MQ=255)                                                                                                                                                                              aTGCGTATCGGCGTGCGGTTGCTGCTGTGCTTGTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGTGTCGTTAATCGATACCGCTACGTTGCTGGCGGAGCTTGCGCGTCCCGATTTGCTCTCTGGGTTCCCAACGCAGGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCgg                                                                  <  2:469456/251‑1 (MQ=255)                                                                                                                                                                                  gTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGc                                                              <  1:36553/251‑1 (MQ=255)                                                                                                                                                                                         cATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACa                                                       <  1:675297/251‑1 (MQ=255)                                                                                                                                                                                               gttgctGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACCCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACCTCGCCCGTTTCGCGCCAATATCGGGGGCATTTACAAAATgc                                                 >  1:291978/1‑251 (MQ=255)                                                                                                                                                                                                ttgctgCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGACGTTAAACCGGGCGTGCGAAGAGCAAAGGAGGGGACGTTAAACGATACCGCAACGTTGCTGGCGGAGCTGGCGCGGCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCCGATCAAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACCAAGTgcg                                                >  1:602867/5‑251 (MQ=255)                                                                                                                                                                                                ttgctgCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGGTGCATTAACAAAGTgcg                                                >  1:596264/5‑251 (MQ=255)                                                                                                                                                                                                    tgCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAAAAAAGTGCGCAAt                                            >  1:656271/1‑251 (MQ=255)                                                                                                                                                                                                     gCTGGGTTATTTTTTACGGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTGCGCGCCAATATCGGTTGCATTAACAAAGTGCGCAATg                                           <  2:446109/251‑1 (MQ=255)                                                                                                                                                                                                        gggCTTTTTTTTACGTGTGGCGGGGGCGGCCTGGTTCGTACTGGCAATTTTTGGCAAAGAAGTTAATCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGTGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGTCGTAGGCGTTTAATCAGCTACAACATCGCCCGTTGCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGaa                                         <  2:379891/250‑1 (MQ=255)                                                                                                                                                                                                        gggCTATTTTTTACTGGGGACGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGGCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATTCCGCAACGTTGCTGGCTGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGaa                                         <  1:60120/250‑1 (MQ=255)                                                                                                                                                                                                        gggCGATTTGTTACTGGTGGCGGTGGGAGGCTGGTTCGTACTGGCCATTTTTGTCAAGGAAGGTAATCCGGGCGTGCGAAGAGCAACGGAGGGGACGGTGATCGACACCGCGACGTTGCTGGCGGAGCTGGCGCGTACCGAGTTGCTCTCTGGGGACCCAACGCATGGGCAACGGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTGCGCGCCAATATCGGTGGGATTAACAAAGTGCGCAATGaag                                        <  2:47234/251‑2 (MQ=255)                                                                                                                                                                                                         ggCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGGACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAata                                       <  1:482146/251‑1 (MQ=255)                                                                                                                                                                                                             aTTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGTGGCATTAACAAAGTGCGCAATGAATATCAt                                   >  1:255465/1‑251 (MQ=255)                                                                                                                                                                                                               tttttACTGGTGGCGGTGGCGGCCTGGTTCGTACTGTCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTa                                                                                                       <  2:379879/181‑1 (MQ=255)                                                                                                                                                                                                               tttttACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTa                                                                                                       >  1:379879/1‑181 (MQ=255)                                                                                                                                                                                                                       gtgggcggtggcGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTTGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTatat                           <  1:275639/246‑1 (MQ=255)                                                                                                                                                                                                                          ggcggtggcGGCCTGGATCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTACCGATTTGCTCTCTGGGGACCCAACGCAGTGGCAAACGGCGCCGGCGTTTAATCAGCTACAAACTTGCCCCTTTCGCGCCAAAATCGGTGGCATTAACAAAAGGGGCCATGACTATCATGTCTTAATGACCg                      >  2:374813/1‑251 (MQ=255)                                                                                                                                                                                                                           gcggtggcGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAATTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGa                     <  1:492521/251‑1 (MQ=255)                                                                                                                                                                                                                              gtggcgtccTGGTTGGTACTGGGTATTTGTGTGATAGAAGTTAAACCGGTGGTTCGAAGAGCAACGGAGTGGCCTTTAAGGGATACCGCAACGTTTCTGGCGTAGCTTGCGCGGCCCGATTTGCGCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCGCGTTTCGCGCCAAGATCGTTGGTATTAACAAAGTGCGCAAGGAATGTCATGTCTATATGCCCggttc                  <  2:111256/244‑5 (MQ=255)                                                                                                                                                                                                                                   ggCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACGGGGAGTGCGAAGAGAAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGGTTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAACGGCGCAATGAATATCATGTCTATATGACCGATTCGCAgg             >  1:565799/2‑251 (MQ=255)                                                                                                                                                                                                                                     ccTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAgggt           <  1:20422/251‑2 (MQ=255)                                                                                                                                                                                                                                      cTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAgggt           >  1:183773/1‑249 (MQ=255)                                                                                                                                                                                                                                      cTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGGTGCTGGCGGAGCTGGCGCGGCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGGTTAATCAGCTACAACATCGACCGTTTCCCGCCAATAACGGTGGCATTAACAACATCCGCAATGAATATCATgacaataaaaccgattcgccgggt           >  2:134192/1‑227 (MQ=255)                                                                                                                                                                                                                                              tACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCCACTGGCGCCGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCCATGAAAATCATGTCTCTATGACCGATTCGCAGGGTAAAGTATTg  >  2:229325/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTG  >  NC_000913/4636523‑4637010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑