Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,591	C→T	V195V (GTC→GTT)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,591	0	C	T	100.0%	194.7 / NA	60	V195V (GTC→GTT)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/28);  total (32/28)

ACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGAC  >  NC_000913/4636355‑4636827                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             aCCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTa                                                                                                                                                                                                                                 >  1:336388/1‑250 (MQ=255)       cTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTACCGTCATCCGGATTGGTCATTTTGGATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTACTGGATAGCGTGTGGGAAGGTGCGCAGGAAACCTACGATCGCACCGTTGATCCCCACCTTAAAACgtt                                                                                                                                                                                                                          >  2:221253/1‑249 (MQ=255)                    ccgccttcccggtcccggctcCCGTCATCCGTATTGGCCATTTTAAATTAAATGAACCCGGGGCGAAGACCATC‑TGGTTGTACACGCCATTAACGCTGCCGCGTTATGAGTTTTTATTGCTGAAGACGTGACTCAAGTCGCAAGGCCGGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTg                                                                                                                                                                                                             <  2:641338/233‑1 (MQ=255)                                 gCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGATGCGTGCCAAGCTGCGCGCCATCAAcc                                                                                                                                                                                                >  2:465711/1‑251 (MQ=255)                                   cctccccctccacctttatggggttttttGAATGGAATGACCCCGGGGGGCAGA‑CAGGGTGGTTTTACCCGCCATTACCGCGGACGCGGTATGAGTTTTTATTGCGGAAGACGTTCCTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAAcccc                                                                                                                                                                                              <  1:624125/226‑1 (MQ=255)                                   cGTCTCCTGCCATCTGTATTGGTCATTTGGTATTGATGGACCCCGCGGCTCAGACCAGC‑TTGTTCTACACGCCATTACCTCTGCCGCGGTATGAGTGTTTCTTCCTGAAGACGTTACACAAGTCGCCTGGCCGCTTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCACcccc                                                                                                                                                                                              <  2:214944/251‑1 (MQ=255)                                   cGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTg                                                                                                                                                                                                             >  1:641338/1‑236 (MQ=255)                                        cccGTCATCCGTATGGGTCATTTTGAATTAAATGACCCCGGGGCGCAGACCAGC‑TGTTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCt                                                                                                                                                                                         <  1:466021/251‑1 (MQ=255)                                             cATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCAc                                                                                                                                                                                    >  1:333231/1‑251 (MQ=255)                                                 cGTATTGGTCATTTTGAATTGAATGACCCCGCGGCGCAGATCAGC‑TGTTTTGACACGCCATTAACGCTGACGCGGTATGATTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGAt                                                                                                                                                                                <  1:465711/251‑1 (MQ=255)                                                  gTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTg                                                                                                                                                                                                             >  1:610809/1‑221 (MQ=255)                                                              ttgaattgaaTGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGCTAGAGTGTGGGAAGATGCGCAGGACCCCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCACGCCATCAACCCCTATCTTTCCCCGATTAATACTCATccc                                                                                                                                                                   >  2:20422/1‑249 (MQ=255)                                                                aaatgaaataaaccgcggaggaaGATCAGT‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTCCTCAATTGGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCgg                                                                                                                                                                 <  1:285371/229‑1 (MQ=255)                                                                 aattgaatGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGTTCTTTCACCGCTTAATACTCATCGCGGc                                                                                                                                                                >  2:117721/1‑251 (MQ=255)                                                                  attgaatGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTactaataatc                                                                                                                                                                     >  1:137616/1‑236 (MQ=255)                                                                     aaatgaaCCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATgg                                                                                                                                                            <  1:100626/250‑1 (MQ=255)                                                                               gcggcgCAGATCGGT‑GGGTTTGACACGCCATTAACGCTGACGCGGTAGGAGTTTTTATTGCTGAAGACGTTACTAAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCt                                                                                                                                                  <  1:401724/251‑1 (MQ=255)                                                                                 tgcgcAGATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGAAATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTg                                                                                                                                                 >  2:178946/2‑250 (MQ=255)                                                                                       gATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGcc                                                                                                                                          >  1:297082/1‑251 (MQ=255)                                                                                       gATCAGC‑TGGTTTGACACGCCATTAACGCTGACGCGGGATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGCTCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTAATCGCGGCATGGGATATAGCCTGAGGGGcc                                                                                                                                          >  2:492521/1‑251 (MQ=255)                                                                                             gatggtagaacacGACATTAACGCTGGCGCGGTAGGAGTTTTTCGTGAGAAAGACGTAACTCAAGTCGCAAGGCCGCGTCTGGACCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTaa                                                                                                                                     <  2:139903/242‑1 (MQ=255)                                                                                                      acacGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCg                                                                                                                            >  1:485272/1‑251 (MQ=255)                                                                                                         cGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCAACATTAAAACGTTGCGTGCCAAGCTGCGCGACATCAACCCCGAACTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCGGTAATGCGTaacgtc                                                                                                                          >  2:408000/1‑245 (MQ=255)                                                                                                          gCCATTAACGCTGACGAGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGACAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGGGCCAAGCAGCGCGCCATCGACCCCGCTCTTTCACCGATTAATACTCATCGCGGCATGGGTAATCGCCTGAGGGGCCTGAAATGCGTATCGGcg                                                                                                                         >  2:223812/1‑249 (MQ=255)                                                                                                          cccATTAACGCTGACGCGTTATGAGTTTTTATTGCTGAAGACTTTACTCAGTTCGCCAGGCCTCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGTAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                                        <  1:221253/250‑1 (MQ=255)                                                                                                               taacgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTCCTCAAGCCGCAAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCgg                                                                                                                    <  1:21529/247‑1 (MQ=255)                                                                                                                accgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGGCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgtt                                                                                                                  <  1:140312/249‑1 (MQ=255)                                                                                                                    cTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGGAATGCGTATCGGCATGCGgttgctg                                                                                                              >  2:675297/1‑248 (MQ=255)                                                                                                                             tATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGAtata                                                                                                                                                      >  1:40353/1‑202 (MQ=255)                                                                                                                                   tttttATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttttttt                                                                                               >  1:438289/1‑250 (MQ=255)                                                                                                                                      ttATTGCTGAAGACTTTACTCAGGTCGCCTGCCGGTCTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATTCGCAGGACCCCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCAGCGCGTCATGGGATAAATCCTGAGGGGCCTTTAATGCGGATCGGCATGCGGTTGCTGCTTGGCTATTTTTTACt                                                                                             <  1:199403/250‑1 (MQ=255)                                                                                                                                              gaagcatttaacaaagtcgctgggCCGCGTCGTGTCCCGCCAGCAACTAATGGATGGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAGACTCAGCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGGATCGGCATGCGGTTGCTGCTGGGCTATTTTTTATTggtggcgg                                                                                     <  2:81994/229‑1 (MQ=255)                                                                                                                                              gAAGACTTTCCTCAGGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcggt                                                                                    <  2:336388/251‑1 (MQ=255)                                                                                                                                                      tACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTg                                                                                                                                                                                                                     >  2:179062/1‑114 (MQ=255)                                                                                                                                                      tACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTg                                                                                                                                                                                                                     <  1:179062/114‑1 (MQ=255)                                                                                                                                                      tACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGTCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGcc                                                                             <  1:468226/250‑1 (MQ=255)                                                                                                                                                            ggcggccGGGCCGCGTCTGTTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGTCACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCg                                                                      <  2:563450/246‑1 (MQ=255)                                                                                                                                                            agtcgccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGCTAGCGTGTGGGCAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTTCTGGTGGCGGTGGCGGCCTGGTTCg                                                                      >  1:578312/9‑251 (MQ=255)                                                                                                                                                              tcgccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGCGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATAGCGGCATGGGATAAAACCTGAGGGGCCTGTAATGCGTATCGACATTCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGACGGGTTCGt                                                                     >  2:304694/7‑250 (MQ=255)                                                                                                                                                                gccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCACGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCgg                                                                                                                           >  2:253933/5‑194 (MQ=255)                                                                                                                                                                gccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCgg                                                                                                                           <  1:253933/190‑1 (MQ=255)                                                                                                                                                                gccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGAAGGACACCTCCGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCCCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGACAGTAATGCGTATCGGCATGCGGTTGCTTATTGGCTATTTTTTACaggtggggggtgggctgtgggtcggc                                                                    >  2:278620/5‑229 (MQ=255)                                                                                                                                                                 ccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGAACTTTCACCGCTTAATACTCATCGCGGCCTGGGATATAGCCTGAGAGGCCTGTAATGCGTATCGGCATGAGGATGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCTGCCGGGGTCGTACTg                                                                 >  2:49543/4‑251 (MQ=255)                                                                                                                                                                  gtggctgcggtgggctccGCCAGCAACTAAGGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGTTGGCGGCCTGGTTCGTACTgg                                                                <  2:432833/235‑1 (MQ=255)                                                                                                                                                                          gTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttttt                                                        >  1:480453/1‑251 (MQ=255)                                                                                                                                                                              ggTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGt                                                      <  1:71973/249‑1 (MQ=255)                                                                                                                                                                                      cAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTgg                                                                                                          <  1:554821/189‑1 (MQ=255)                                                                                                                                                                                      cAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTgg                                                                                                          >  2:554821/1‑189 (MQ=255)                                                                                                                                                                                             taaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCTGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGCGGCGGGCTGGTTCGTACTGGCTATTTTTGTCAAATAAGGTAAAca                                       >  1:423201/3‑248 (MQ=255)                                                                                                                                                                                                  gATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgtt                                                                                                                  >  1:210079/1‑169 (MQ=255)                                                                                                                                                                                                  gATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgtt                                                                                                                  <  2:210079/169‑1 (MQ=255)                                                                                                                                                                                                      gCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGaa                            <  1:665260/251‑1 (MQ=255)                                                                                                                                                                                                          gtGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCaa                                                                                                                                                                                                  <  2:478357/80‑1 (MQ=255)                                                                                                                                                                                                          gtGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCaa                                                                                                                                                                                                  >  1:478357/2‑81 (MQ=255)                                                                                                                                                                                                            gggAAGATGCGCAGGAAACCTACGATCGCACCGTTGATACCCACATTAAAACGTGGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCaa                      <  2:542961/251‑1 (MQ=255)                                                                                                                                                                                                              gcaGATGCGCAGCACACCTACGATCGCACCGTTGATACCCACATTAAAACGATGCGTGCCAAGCTGAGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGACATGGAATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGATGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTATTGGGTATTTTTGACAAGGAAGATAAAACgggggagctgagaggaacg                    >  2:580329/3‑235 (MQ=255)                                                                                                                                                                                                              gAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGTTTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACg                    >  1:518576/1‑251 (MQ=255)                                                                                                                                                                                                              gAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCTGGCGTGCGAAGAGCAACg                    <  1:178946/251‑1 (MQ=255)                                                                                                                                                                                                                aGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCGTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCGGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGa                  <  2:438289/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTTAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGGGGGGGCGTTAATCgca  >  2:256099/1‑248 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             ACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGC‑TGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGAC  >  NC_000913/4636355‑4636827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑