Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,960 T→C D76D (GAT→GAC slt → soluble lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,9600TC93.2% 160.0 / ‑1.5 59D76D (GAT→GACsltsoluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (30/25);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

TAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACC  >  NC_000913/4630719‑4631206
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                              
tAGAGGATGCGCTTGGGGAAAAAGCCAAACAAGTTACCTGGCGGGTGTTGGCTGTCGGTGTCTGTCTGCTGACGGTCAGGAGCGTGGCGCGAGCCGACTCCCTGGATGAGCAGCGTAGCCGTTACGCGAAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATCCCGTCAGATCACCGATGACCtgatga                                                                                                                                                                                                                                                         <  1:126402/249‑1 (MQ=255)
 agagGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATc                                                                                                                                                                                                                                                      >  1:559521/1‑251 (MQ=255)
      aTGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcg                                                                                                                                                                                                                                                 <  1:496349/251‑1 (MQ=255)
             tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGACTGCTGACGGTCAGCAGCGTGGCGCGAGCCGCCTCACTGGATGAGCAGCGTAGCAGTTACGCGCAAATCATGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAAAGATGCCTGGACTGAAAGATTATCCACATTATCCCTACCTGGCATACCGTCAGATCACAGATGACCTGGTGAAACAAAcggcag                                                                                                                                                                                                                                             >  2:366454/1‑246 (MQ=255)
              gtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGACGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTCCCTGGAATACCGTCAGGTCACCGATGACCTGATGAATCAACCGGCGGTg                                                                                                                                                                                                                                           >  2:82426/1‑249 (MQ=255)
              ggggaaaaccccaacaaaGTTACCTGGCGGGTGTGGGCGTCCGGTGTCGGTCTTCTGACGGTCAGCAGCTTGGAGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCACATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAATATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAc                                                                                                                                                                                                                                        <  2:589543/235‑1 (MQ=255)
                ggAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGTCTGCCGGTGCCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGActg                                                                                                                                                                                                                                      <  1:352323/251‑3 (MQ=255)
                ggAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGActg                                                                                                                                                                                                                                      <  1:461966/251‑3 (MQ=255)
                             cAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCCCTAAATTTGt                                                                                                                                                                                                                        >  2:20449/1‑251 (MQ=255)
                              aaGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAACTCAAGCAGGCCTGGGATAATCGACAAATGTATGTGGTCGAACAAATGCTACCTGGACTGCAAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGTTGAATCAACCGGCGGTAAATGGTACTAACTTTGtt                                                                                                                                                                                                                       >  2:353660/1‑251 (MQ=255)
                               aGTTACCGGGCGGCTGTTGGCTGCCGGTGTCTGTTGGCTGACGGTCAGGAGCGTGTCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCTGCGGTTACTGTCACTAAGTTTGTTc                                                                                                                                                                                                                      <  2:598542/251‑1 (MQ=255)
                                      tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCCCTAACTTTGTTCGCGCTa                                                                                                                                                                                                                >  1:353216/1‑250 (MQ=255)
                                        gcggcTGTTGGCTGCCGGTTTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatga                                                                                                                                                                                                                                                         <  2:364474/209‑1 (MQ=255)
                                        gcggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatga                                                                                                                                                                                                                                                         >  1:364474/1‑209 (MQ=255)
                                           gcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCa                                                                                                                                                                                                          <  1:20449/251‑1 (MQ=255)
                                             tGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGAACGTCACTAACTTTGTTGGCGCTTACCCCAcc                                                                                                                                                                                                        >  2:373474/1‑250 (MQ=255)
                                                tGGCTGCGGGTGTCTGTATGCTGACGGTCCGCAGCGTGGCGCGAGACGCCTACCTGGATGAGCAGCGTAGCCGTTAAGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACACATGATGCCTGGACTGACAGGTTATCAAATTTATCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGCTGAATACACCGGCGGTGTCTGTGCATAACTTTGTTCGCTCTAAACCCACGcat                                                                                                                                                                                                     >  2:335787/1‑249 (MQ=255)
                                                     gccgggggcggcctgctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTTGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGTGGACTGTCTCTAACTTTGCTCGCGCTAACCCCACGCTtccg                                                                                                                                                                                                  <  2:248744/237‑2 (MQ=255)
                                                           gtctgtctGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACTCTTCCTCCCGCTCg                                                                                                                                                                                          <  1:560023/251‑1 (MQ=255)
                                                            tctgtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGc                                                                                                                                                                                         >  1:618920/1‑251 (MQ=255)
                                                                    ctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGAGGTGACTGTCACTAACTTTGTTATCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGcc                                                                                                                                                                                 >  2:627777/1‑250 (MQ=255)
                                                                            cagcagCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGCAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGCTGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGtt                                                                                                                                                                         >  1:279981/1‑251 (MQ=255)
                                                                                agcGTGGCGCGAACCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACATGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTACCGCTCGCACGCTGCCATCTCGTTTcat                                                                                                                                                                     >  1:318062/1‑249 (MQ=255)
                                                                                       cgcgAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGCCTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGGCACTAACTTTGTTCACGCTAACCCCACGATTCCTCCCGCTCGAAAGCTGCAATCTCGTTTCGTCAATGa                                                                                                                                                               >  1:1980/1‑250 (MQ=255)
                                                                                           aGCCGACTCACTGGATGAGCCGCGTAGCCGTTACGCGCAAATCAAGCAGTCCTTGGATAAACGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTTAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTgg                                                                                                                                                          <  2:87369/251‑1 (MQ=255)
                                                                                              cGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgcgc                                                                                                                                                       <  1:353660/251‑1 (MQ=255)
                                                                                                    aCTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTg                                                                                                                                                 <  1:510282/251‑1 (MQ=255)
                                                                                                           gAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCATGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTg                                                                                                                                          >  1:223674/1‑251 (MQ=255)
                                                                                                              cagcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCg                                                                                                                                       <  1:373474/251‑1 (MQ=255)
                                                                                                                     gccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTg                                                                                                                                >  1:412412/3‑251 (MQ=255)
                                                                                                                      ccGTTACGCTCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTTGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCTCCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGt                                                                                                                               <  2:321099/250‑1 (MQ=255)
                                                                                                                         ttACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCACTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGGGGCtttttct                                                                                                                            >  2:533931/1‑246 (MQ=255)
                                                                                                                                  aaTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGcc                                                                                                                 <  1:660107/251‑1 (MQ=255)
                                                                                                                                    ccAAGCAGGCCTGGGATAATCGACAAATGGATGTGGGCGAACAAATGATGCCTGGACTGAAAGATTATCCCCTTTATCCCTACCTGGAATACCGTCAGATCACCGATTACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGccag                                                                                                               <  2:538987/250‑3 (MQ=255)
                                                                                                                                       aGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGaa                                                                                                           <  1:533939/251‑1 (MQ=255)
                                                                                                                                             ccTGGGATAAGCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCGACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCGCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGccc                                                                                                     <  2:193017/251‑1 (MQ=255)
                                                                                                                                                       acgtcAAATGGATGTGGTCGAACAAATGATGCCTGGACTGGAAGATTATCCGCTTTATCCCTACCTGGAATACCGTCAGACCGCCGACGACCTGATGATTCAGCCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCGCGCTTCCGCCCGCTCGCACGCTGCAATCTCGGTTCGTGAATGCACCGGCGTTTCGTGAGCAGTGGCGTGTATTGTTAGCGTTTAGCCCGGAAAGGCCGGGatctgccg                                                                                           <  2:318266/247‑8 (MQ=255)
                                                                                                                                                        cGACAAATGGATGTGGACGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACCAACTTTTGTCCCGCTAACCCCAAGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGACAATGAACTGGGGCGGCGTGAGGGAGGGCGTGGCTTTTTAGGCCTTTTACCCGGaaaaaccgggacaccatg                                                                                           >  2:178438/1‑237 (MQ=255)
                                                                                                                                                          aCAAAGGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAAGCCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAg                                                                                        <  2:353216/251‑1 (MQ=255)
                                                                                                                                                           cAAATTGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAgc                                                                                       <  2:286030/251‑1 (MQ=255)
                                                                                                                                                                      gggcGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAAtt                                                                          <  1:294110/248‑1 (MQ=255)
                                                                                                                                                                       gTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGACCTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAACAGACCGGAACTACCGAAGCGCACAGTAAtt                                                                          >  1:51851/1‑250 (MQ=255)
                                                                                                                                                                         cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTTTTCTCCTTTATCACCGGAAAGACCCGGACAACCCAAGCGCCATGTAAAtact                                                                       >  2:287449/1‑251 (MQ=255)
                                                                                                                                                                         cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTTTTAGCCTTTTGCCCGGAAAAACCCGGGACCACCAAAGCGCattgaacttact                                                                         >  2:447467/1‑240 (MQ=255)
                                                                                                                                                                                atgatgCCTGGACTGAAGGATTATCCGCTTTATCCCTCCCTGGAATACCGCCAGATCACCTATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATg                                                                                                                                                                <  2:240342/160‑1 (MQ=255)
                                                                                                                                                                                atgatgCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATg                                                                                                                                                                >  1:240342/1‑160 (MQ=255)
                                                                                                                                                                                  gatgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCCATGTAATTACTACTATGca                                                               >  1:358856/1‑249 (MQ=255)
                                                                                                                                                                                   atgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTTGCCCGGGAAAACCCCGGACTAACGGAGCGCCATGGAATTActactaagcaa                                                              >  1:2578/1‑245 (MQ=255)
                                                                                                                                                                                         ggACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCCAAATggg                                                        >  1:603647/1‑249 (MQ=255)
                                                                                                                                                                                            cTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTCACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGCACCGGCGCGGCGTGAAGACTGGCGTTGCTTGTTTACCTTTAGCCCGGGCAAGCCCGGGACCACCGCAGAGCAATGTACTTACTAATATTCAAAACGGAacac                                                    >  2:651609/1‑251 (MQ=255)
                                                                                                                                                                                               gaaagATTATCCACTTTATCCCTACCTGGAATCCCGTCAGATCACCGATGACCTTATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTGCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCTGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACAcc                                                   <  1:471997/246‑1 (MQ=255)
                                                                                                                                                                                               aaGGATTATCCCCCTTATCCCTGCCTGGAATTCCGTCAGATCCCCGATGATCTGATGAAGCAACCGGCGGTGACTGCCACTAACTTTGTTCGCTCTAACCCTACGCTTCCTCCCGCTCGCACGCTGCAATCTCGCTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGTCTTTAGCCCGGAAAAGCCAGGAACTGCCGAAGCGCAATGTAATTACTACTATGCCAAATggtccaccgt                                                 <  2:1980/251‑9 (MQ=255)
                                                                                                                                                                                                  agagtctCCGCTGTATCTCTACCTGGAATACCGACATATCACCGATGACCTGATGAATCATTCGGCGGTGACTGGCACTAACCTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCATTCTCGTTTCGTCAAGGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGTCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGc                                               <  2:181659/245‑1 (MQ=255)
                                                                                                                                                                                                   gATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTACCTTTAGACCGGAAAAGACCGGGACTAACGAAGAGCACTTGTATTAATACTATGCAAAAAGGAaccacgggc                                               >  1:410801/1‑243 (MQ=255)
                                                                                                                                                                                                        tccacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGGAGTACAGAAGCGCAATTTAATTACTACTATGCCAAATTGAACAACCGGCAGGGTg                                         >  1:488034/5‑251 (MQ=255)
                                                                                                                                                                                                                       ccTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATATAATTACTACTATGCAAAATGGAACACCGGGCAGAGGGAAAAAACCTGGCa                            >  1:145881/1‑249 (MQ=255)
                                                                                                                                                                                                                          ggAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAgggg                      <  1:673734/251‑1 (MQ=255)
                                                                                                                                                                                                                             aTACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCg                    >  1:165437/1‑250 (MQ=255)
                                                                                                                                                                                                                                        tCACCGATGACCTGATGAATCAACCGGCGGTTACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCACTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGAGTGGCTTGTTTGTATTTAGCCCGGGAAAACCCGGGAATACCGAAACGCAATTGCAATAATAATATGCAAAAAGGAAAACCGGGCAGAGGGAAGAAACCCGGCAAAGGGGGGAAaggctgagg         >  2:645864/1‑244 (MQ=255)
                                                                                                                                                                                                                                              atgacCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAAcc  <  1:82426/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                              
TAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACC  >  NC_000913/4630719‑4631206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: