| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,630,945 | C→T | R71R (CGC→CGT) | slt → | soluble lytic murein transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,630,945 | 0 | C | T | 95.1% | 164.8 / ‑0.2 | 62 | R71R (CGC→CGT) | slt | soluble lytic murein transglycosylase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (31/27); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
ATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGA‑CGGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCG > NC_000913/4630702‑4631188 | aTTGATTTTTTTCCACTTTGAGTATGCGCTGGTGGAAAAAGCTAAAAAAGTTACCTGGCGGCTGGTGGCTGCCGGTGTCTGTCTGTTTACGTGCAGCATCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATAAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTAGCCCTACCTTGAAGACCGTCAGATCa < 2:477726/251‑1 (MQ=255)aTTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTATGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAgctc > 1:627631/1‑247 (MQ=255)aTTGATGTATTTACACTTAGAGGAGGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTTCCTGGAATACCGTCAGATCa < 1:464466/251‑1 (MQ=255) gATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACACACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCg > 2:71199/1‑251 (MQ=255) tAGAGGATGCGCTTGGGGAAAAAGCCAAACAAGTTACCTGGCGGGTGTTGGCTGTCGGTGTCTGTCTGCTGACGGTCAGGAGCGTGGCGCGAGCCGACTCCCTGGATGAGCAGCGTAGCCGTTACGCGAAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATCCCGTCAGATCACCGATGACCtgatga < 1:126402/249‑1 (MQ=255) agagGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATc > 1:559521/1‑251 (MQ=255) aTGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcg < 1:496349/251‑1 (MQ=255) tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGACTGCTGACGGTCAGCAGCGTGGCGCGAGCCGCCTCACTGGATGAGCAGCGTAGCAGTTACGCGCAAATCATGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAAAGATGCCTGGACTGA‑AAGATTATCCACATTATCCCTACCTGGCATACCGTCAGATCACAGATGACCTGGTGAAACAAAcggcag > 2:366454/1‑246 (MQ=255) gtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGACGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTCCCTGGAATACCGTCAGGTCACCGATGACCTGATGAATCAACCGGCGGTg > 2:82426/1‑249 (MQ=255) ggggaaaaccccaacaaaGTTACCTGGCGGGTGTGGGCGTCCGGTGTCGGTCTTCTGACGGTCAGCAGCTTGGAGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCACATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AATATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑c < 2:589543/235‑1 (MQ=255) ggAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGTCTGCCGGTGCCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑ctg < 1:352323/251‑3 (MQ=255) ggAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑ctg < 1:461966/251‑3 (MQ=255) cAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGA‑CTGT‑CCCTAAATTTGt > 2:20449/1‑251 (MQ=255) aaGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAACTCAAGCAGGCCTGGGATAATCGACAAATGTATGTGGTCGAACAAATGCTACCTGGACTGC‑AAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGTTGAATCAACCGGCGGTAAATGGT‑‑ACTAACTTTGtt > 2:353660/1‑251 (MQ=255) aGTTACCGGGCGGCTGTTGGCTGCCGGTGTCTGTTGGCTGACGGTCAGGAGCGTGTCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCTGCGGTTA‑CTGT‑CACTAAGTTTGTTc < 2:598542/251‑1 (MQ=255) tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CCCTAACTTTGTTCGCGCTa > 1:353216/1‑250 (MQ=255) gcggcTGTTGGCTGCCGGTTTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatga < 2:364474/209‑1 (MQ=255) gcggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatga > 1:364474/1‑209 (MQ=255) gcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCa < 1:20449/251‑1 (MQ=255) tGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGA‑ACGT‑CACTAACTTTGTTGGCGCTTACCCCAcc > 2:373474/1‑250 (MQ=255) tGGCTGCGGGTGTCTGTATGCTGACGGTCCGCAGCGTGGCGCGAGACGCCTACCTGGATGAGCAGCGTAGCCGTTAAGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACACATGATGCCTGGACTGACAGG‑TTATCAAATTTATCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGCTGAATACACCGGCGGTGT‑CTGTGCA‑TAACTTTGTTCGCTCTAAACCCACGcat > 2:335787/1‑249 (MQ=255) gccgggggcggcctgctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTTGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGTGGA‑CTGT‑CTCTAACTTTGCTCGCGCTAACCCCACGCTtccg < 2:248744/237‑2 (MQ=255) gtctgtctGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACTCTTCCTCCCGCTCg < 1:560023/251‑1 (MQ=255) tctgtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGc > 1:618920/1‑251 (MQ=255) ctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGAGGTGA‑CTGT‑CACTAACTTTGTTATCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGcc > 2:627777/1‑250 (MQ=255) cagcagCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGCAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGCTGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGtt > 1:279981/1‑251 (MQ=255) agcGTGGCGCGAACCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACATGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTACCGCTCGCACGCTGCCATCTCGTTTcat > 1:318062/1‑249 (MQ=255) cgcgAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGCCTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGG‑CACTAACTTTGTTCACGCTAACCCCACGATTCCTCCCGCTCGAAAGCTGCAATCTCGTTTCGTCAATGa > 1:1980/1‑250 (MQ=255) aGCCGACTCACTGGATGAGCCGCGTAGCCGTTACGCGCAAATCAAGCAGTCCTTGGATAAACGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTTA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTgg < 2:87369/251‑1 (MQ=255) cGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgcgc < 1:353660/251‑1 (MQ=255) aCTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTg < 1:510282/251‑1 (MQ=255) gAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCATGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTg > 1:223674/1‑251 (MQ=255) cagcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCg < 1:373474/251‑1 (MQ=255) gccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTg > 1:412412/3‑251 (MQ=255) ccGTTACGCTCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTTGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCTCCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGt < 2:321099/250‑1 (MQ=255) ttACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCACTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGGGGCtttttct > 2:533931/1‑246 (MQ=255) aaTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGc‑c < 1:660107/251‑1 (MQ=255) ccAAGCAGGCCTGGGATAATCGACAAATGGATGTGGGCGAACAAATGATGCCTGGACTGA‑AAGATTATCCCCTTTATCCCTACCTGGAATACCGTCAGATCACCGATTACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGc‑cag < 2:538987/250‑3 (MQ=255) aGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑aa < 1:533939/251‑1 (MQ=255) ccTGGGATAAGCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AGGATTATCCGCTTTATCCCGACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGA‑CGGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCGCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGccc < 2:193017/251‑1 (MQ=255) acgtcAAATGGATGTGGTCGAACAAATGATGCCTGGACTGG‑AAGATTATCCGCTTTATCCCTACCTGGAATACCGTCAGACCGCCGACGACCTGATGATTCAGCCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCGCGCTTCCGCCCGCTCGCACGCTGCAATCTCGGTTCGTGAATGCACCGGCGTTTCGTGAGCAGTGGCGTGTATTGTTAG‑CGTTTAGC‑CCGG‑AAAGGCCGGGatctgccg < 2:318266/247‑8 (MQ=255) cGACAAATGGATGTGGACGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACCAACTTTTGTCCCGCTAACCCCAAGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGACAATGAACTGGGGCGGCGTGAGGGAGGGCGTGGCTTTTTAGGCCTTTTAC‑CCGG‑aaaaaccgggacaccatg > 2:178438/1‑237 (MQ=255) aCAAAGGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAAGCCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAg < 2:353216/251‑1 (MQ=255) cAAATTGATGTGGTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAgc < 2:286030/251‑1 (MQ=255) gggcGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAAtt < 1:294110/248‑1 (MQ=255) gTCGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGACCTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AACAGACCGGAACTACCGAAGCGCA‑CAGTAAtt > 1:51851/1‑250 (MQ=255) cGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTTTTCT‑CCTTTATCACCGG‑AAAGACCCGGA‑CAACCCAAGCGCC‑ATGTAAAtact > 2:287449/1‑251 (MQ=255) cGAACAAATGATGCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTTTTAG‑CCTTTTGC‑CCGG‑AAAAACCCGGGACCACCAAAGCGCattgaacttact > 2:447467/1‑240 (MQ=255) atgatgCCTGGACTGA‑AGGATTATCCGCTTTATCCCTCCCTGGAATACCGCCAGATCACCTATGATCTGATGAATCAACCGGCGGTGA‑CGGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATg < 2:240342/160‑1 (MQ=255) atgatgCCTGGACTGA‑AGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGA‑CGGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATg > 1:240342/1‑160 (MQ=255) gatgCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCC‑ATGTAATTACTACTATGca > 1:358856/1‑249 (MQ=255) atgCCTGGACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTG‑CCTTTTGC‑CCGGGAAAACCCCGGA‑CTAACGGAGCGCC‑ATGGAATTActactaagcaa > 1:2578/1‑245 (MQ=255) ggACTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCCAAATggg > 1:603647/1‑249 (MQ=255) cTGA‑AAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTCACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGCACCGGCGCGGCGTGAAGACTGGCGTTGCTTGTTTA‑CCTTTAGC‑CCGG‑GCAAGCCCGGGACCACCGCAGAGCA‑ATGTACTTACTAATATTCAAAACGGAacac > 2:651609/1‑251 (MQ=255) gaaagATTATCCACTTTATCCCTACCTGGAATCCCGTCAGATCACCGATGACCTTATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTGCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CTGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCAAAATGGAACAcc < 1:471997/246‑1 (MQ=255) a‑aGGATTATCCCCCTTATCCCTGCCTGGAATTCCGTCAGATCCCCGATGATCTGATGAAGCAACCGGCGGTGA‑CTGC‑CACTAACTTTGTTCGCTCTAACCCTACGCTTCCTCCCGCTCGCACGCTGCAATCTCGCTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTG‑TCTTTAGC‑CCGG‑AAAAGCCAGGAACTGCCGAAGCGCA‑ATGTAATTACTACTATGCCAAATggtccaccgt < 2:1980/251‑9 (MQ=255) agagtctCCGCTGTATCTCTACCTGGAATACCGACATATCACCGATGACCTGATGAATCATTCGGCGGTGA‑CTGG‑CACTAACCTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCATTCTCGTTTCGTCAAGGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGTCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCAAAATGGAACACCGGGc < 2:181659/245‑1 (MQ=255) gATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTA‑CCTTTAGA‑CCGG‑AAAAGACCGGGACTAACGAAGAGCACTTGTA‑TTAATACTATGCAAAAAGGAaccacgggc > 1:410801/1‑243 (MQ=255) tccacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGGAGTACAGAAGCGCA‑ATTTAATTACTACTATGCCAAATTGAACAACCGGCAGGGTg > 1:488034/5‑251 (MQ=255) ccTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATATAATTACTACTATGCAAAATGGAACACCGGGCAGAGGGAAAAAACCTGGCa > 1:145881/1‑249 (MQ=255) ggAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAgggg < 1:673734/251‑1 (MQ=255) aTACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGA‑CTGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCg > 1:165437/1‑250 (MQ=255) | ATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGA‑AGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGA‑CGGT‑CACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAG‑CCTTTAGC‑CCGG‑AAAAGCCCGGAACTACCGAAGCGCA‑ATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCG > NC_000913/4630702‑4631188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |