| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,630,238 | T→A | S95S (TCA→TCT) | ettA ← | energy‑dependent translational throttle protein EttA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,630,238 | 0 | T | A | 97.4% | 233.2 / ‑2.0 | 76 | S95S (TCA→TCT) | ettA | energy‑dependent translational throttle protein EttA |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base A (31/43); total (33/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
GCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGAC > NC_000913/4629991‑4630467 | gcagcggGCGGTACAACGCTACGCGACGACGCTCACCCCCGG‑GACGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGGTTCAGGGCGTTAACCACTTCAGaa < 2:497194/251‑1 (MQ=255) agGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGc > 1:605671/1‑251 (MQ=255) ggcggcACAACGCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGct < 1:80757/251‑1 (MQ=255) gcggcACAACGCTACGCGACGACGCTCCCCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACTTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGctt < 1:494548/251‑1 (MQ=255) cggcACAACGCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCCGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGAACGTCGTGAGCCTGCATGCTCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGCACAGCGCATACACTTCATCCAGGCGTTTCTGGGCGTTAACCACTTCAGAAACCGcttc > 2:618337/1‑251 (MQ=255) ggcACAACGCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGcttct < 1:324613/251‑1 (MQ=255) ggcACAACGCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGcttct > 1:675243/1‑251 (MQ=255) acaACGCTACGCGACGACGCGCACCCCCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCa < 1:70242/251‑1 (MQ=255) aaaaagCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCa < 1:654461/246‑1 (MQ=255) caACGCTACGCGACGACGCTCACCACCGGAGA‑GGGTAGCGCTTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGATATGACCGTCGTGCGCCTGAATGATCTCTTCCAAGCTGCCTTGTTCAACGGCCAGCTTGTCAAAATTGGCATCCGGATCGGCGGACAGCGCATCCACTGCTTCCAGGCGTTTCAGGGCTTTAACCCCTTCAGAACCCTCTTCTTCac > 2:328420/1‑250 (MQ=255) gCTACGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATgg > 1:74999/1‑250 (MQ=255) caCGCGACGACGCTCACCACCGGAGA‑GGTTAGCGATTTTCGCTTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACt < 2:202063/250‑1 (MQ=255) ccgcgGCGACGCTCACCACCGGGTA‑GCTTAGCGGTTTGCGCTGCCCAGTCCGGCAGACGGTGCGAATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCGTAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTACCCACTTCAGAAACCGCTTCTTCAATGGAc < 2:416434/248‑1 (MQ=255) cgcgACGACGCGCACCACCGGAGA‑GGATAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCCGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTACAGACGGCCTTGTTCAGCGGCCAGCTTGTCAACATCGGCATCCAGATCGGCGAACAGCGCATACACTTCCTCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTc > 2:60363/1‑250 (MQ=255) acgctCACCACCGGAGA‑GGTCAGCGATTCTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGAACgg < 1:248475/247‑1 (MQ=255) ccaccacCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTGCAGAAACCGCGTCTGCAATGGACTCACGCACGgtgtg < 1:529548/250‑1 (MQ=255) gaccccaggAGG‑GGTTAGCGTGTTTCGCGTCCCAGTCCGGCAGACGTGGCG‑ATCCCGCCGCCCACTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCGCTGCCAGACGGCCGTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTGCAATGGACTCACGCACGgtgt < 2:204135/242‑1 (MQ=255) caccGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTcc < 1:190723/251‑1 (MQ=255) accGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAACTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCCTCCAGGCGTTTCAGGGCGGTAACCACTTCAGAAACCGCTTCTTCAATGGACTAAAGCACGGTGTGTTc > 2:656151/1‑249 (MQ=255) ccGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGAACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACCCTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTACgg > 1:68022/1‑251 (MQ=255) ccGGAGA‑GGGTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCgg > 1:144671/1‑251 (MQ=255) cGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCggg < 1:28041/251‑1 (MQ=255) ggAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGt > 1:360108/1‑251 (MQ=255) aga‑gGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGAATACCCTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTACCGCACggggacgtccggtgtc > 2:257040/1‑237 (MQ=255) gatgttatcggttttCGCGTCCCCGTCCGGCAGACGTGGCGCAGCC‑GCCGCACGCGCCATCTGTGCGTTCTGGTTAGGCCCGTCGTGAGCCTGAAGTAGCTCTTCCATACGGCCGTGTTCAGTGTCGAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATGCACTTCATCCAGGCGTTTCAGGGCGTGATCCTCTTCAGAAACCGCTTCTTCAATGGACTTACGCACGGTGTGTTCCTGGTTCa < 2:606293/240‑1 (MQ=255) gTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCt > 1:584174/1‑251 (MQ=255) ttAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGATATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTCACCACTTCAGCAACCGCTTCTTCAATGGACTCTCACACGGTGTGTTCCGGGTTCAACTg > 1:391097/1‑251 (MQ=255) aGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCg < 1:440871/251‑1 (MQ=255) atttttccgTCCCCGTCCGGCAGACTTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGGTTAGTTTATGACCGTAGTGAGCCTGAAGTATCGCTTCCATACGGCTTTGTTCAGCGGCCAGCTTGTCAAAATCGGCAGCCGGATCGGCGTACAGTGCATTCACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTCCTTCAATGGACTCACGCTCGGTGTGTTCCGGTTTCAGCTGGGGTTc < 1:517269/244‑1 (MQ=255) tCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTg < 1:640754/250‑1 (MQ=255) gcgTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCgg < 1:68692/251‑1 (MQ=255) cccAGGCCTGCAGACGTAGCGCATCC‑GCCGCCCGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCGCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGCTCGGCGTACAGCGCATACACTTCATCCAGGCTTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑gg < 2:311188/251‑2 (MQ=255) ccAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑ggg > 1:266821/1‑249 (MQ=255) ccAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACAGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAACCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑ggg > 1:401763/1‑249 (MQ=255) gTCCGGCAGACGTGGCGGATCC‑GCCGCTCGCCCCAGCTGTACGTTCAGGTTATGACCGTCGTGGGCCTGAATTGTCCTTTCCAGACGGCCTTGTTCAGCTGCCAGCTTGGCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCTGGTTCAGCTGCGGTTCCTGCTGCA‑ggttac < 2:459420/251‑6 (MQ=255) gTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAAc < 1:173131/251‑1 (MQ=255) cgtcAGACGTTGCGCTTCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGAACTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTGCAGCTGCGGTTCCTGCGGCA‑GGTAACCaa < 2:675243/248‑1 (MQ=255) gCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACAACTTCAGAAACCGCTTCTTCACTGGACTAACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCACGA‑AACCAATc > 2:551516/1‑251 (MQ=255) cAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCt < 1:12626/251‑1 (MQ=255) cGTAGCGCATCC‑GCCGCACGCTCCAGCTGTCCGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACAGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑gggaaccacttctttt > 2:542660/1‑236 (MQ=255) aGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTc < 1:504556/251‑1 (MQ=255) cgcATCC‑GCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTg < 1:58905/251‑1 (MQ=255) cATCC‑GCCGCACGCTCCAGCTGTACGTTCATGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCTCTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTGCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGc < 2:563724/251‑1 (MQ=255) cgcACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GATAACCAATCTTGATGTCTGGCTGCGGAc > 2:113674/1‑251 (MQ=255) catcctgCTGTACCTTCCGGTTATGACCGTCGTGCGCCTGAATGATCTCTTCCAGACGGGCTTGTTCAGCGGACATATTTTCAAAAACGTCTACCGGATCGGCGTACAGCGCATACTCTTCATCCAGGCGTTTCAGGGCGTTAACCACCTCAGAAACCGCTTCTTCAATGGACGCACGCTCGGTGTGTTCCGGGTTCAGCTGCGGTCCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTTCGGTCGCGCt < 2:245645/245‑1 (MQ=255) gCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCt < 1:51222/251‑1 (MQ=255) gTACGTTCAGGTTATGACCGTCGTGAGCCTGAATTATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGTCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCtt < 1:430502/249‑1 (MQ=255) ttCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTc > 1:605704/1‑251 (MQ=255) caggttATGACCGTCGTGATCTTTAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACCCGTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCtt < 2:124439/247‑1 (MQ=255) cagggtttgacctcccggAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCTGCCATATTGTCAAAATCGGCATCCGGCTCGGCGTACAGCGCATTCACTTAATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGGTTCTTCAATGGACTTACGCTCGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCCATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCtt < 2:74999/234‑1 (MQ=255) aTGATCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCa > 1:36638/1‑250 (MQ=255) aTGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCa < 1:380473/250‑1 (MQ=255) tGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGGATCCCATCTTGATGTCTTGCTGCGAGCGCGCtgtccctttggattctttcacca > 2:626187/1‑228 (MQ=255) ccgccgtGTGCCTTAATGATCTCTCCCAGACGGCTTGGTTCAGCGGCCAGCTTGTCAAAATCTGCATCCGGATCGGCGTACAGCGCATACCCTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACTCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGcc < 2:331740/247‑1 (MQ=255) cggcctgatcccgAATGATCGCTTCCAGACGGCCTTGTTCAGCGGCCATCTTGTCAAAATCTGCATCCGTATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACGTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCTGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGccc < 2:244712/239‑1 (MQ=255) gtGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATgg > 1:627347/1‑140 (MQ=255) gtGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATgg < 2:627347/140‑1 (MQ=255) tGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCgcagc > 1:280636/1‑251 (MQ=255) aGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCt > 1:556361/1‑183 (MQ=255) aGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAACCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCt < 2:556361/183‑1 (MQ=255) ccaccTTGTCAAACCCGGCACCCGGACCGGCGTACGGCGCATCCCCTCCACCCGGGCGTTCCAGGGGTTTACCCACTCCAGAACCCGCTTCTTCAAGGGCCTCACGCAGGGTGTGTTCCGGGTTCAGCTGCGTTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGCCGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAccc < 1:531106/247‑1 (MQ=255) gTCAAAATCGGCCTCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCATTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAAACAATCTTGATGTCTGGCTGCGGCCGCGCCTCACCTTCGATGTCTTTCTCAATCCCCCCCATAATGCGACGCAGGGTAGAATTGCCCGCGCCGTTCAGAcaccgggccc > 2:202344/1‑242 (MQ=255) ggtgcGGCGTACAGCGCATACCCTTCACCCAGGCTTTTCAGGGCTTTAACCACTTCAAAACCCGTTTCTTCAATGGACTCCCGCACGGTGTGTTCCGGGTTCAGCGGCGTTTCCTGCGGCA‑GTTAACCAATCTTTATGTCTGGCGGCGGCCCCGTTTCCCCTTCGATTTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAgg < 1:202344/247‑1 (MQ=255) ccGGCGTACAGCGCAACCCCTTCATCCAGGCGTTCCAGGGCGTTACCCCCTCCAAAACCCTCTTCTTCAATGGACCCCCGCAGGGTGTGTTCCGGGTTCAGCTGCGTTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGgaa < 1:618337/250‑1 (MQ=255) ggCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGt > 2:431174/1‑95 (MQ=255) ggCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGt < 1:431174/95‑1 (MQ=255) gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGAAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAAc > 1:55247/1‑251 (MQ=255) tACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCATTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGCAACCCATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGACGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTCGACATTCCCCCACCCATCAAACCCAGGCCCCCAAATTTTACCCCAggggaaaaacc > 2:363447/1‑243 (MQ=255) cAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGGAACCAATCTTGATGTCTGGCTGCGGAAGCGCTTCACCTTCGATGTCTTTATTAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCCCCGTTCAGACCCAGGACACCCATTTTTTCCCCCGGGGAGAAACTcc > 2:329570/1‑250 (MQ=255) aTACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCCATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGGCTTTTTCAATGCCCGCCATAATGCGCACCAGGGTAGGCTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACTCcgag > 1:275853/1‑248 (MQ=255) aTACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGGAAACAATCTTGATGTCTGGCTGCGGACGCGCCTCACCTTCGGTGGCTTTATCAATGCCCGCCATAATGCGCCGCAGGGTAGACTTACCCCCGCCGTTCAGGCCCAGGACACCAATTTTTGTCCCAGGGAAGaaaatccgagggg > 2:342870/1‑241 (MQ=255) cTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGttt > 1:141965/1‑251 (MQ=255) ccATCCAGGCGTTTAAGGGCGTTAACCCCTACAGAACCCGCTTCTTCATGGGCCTCACGCGCGGTGTGTTCGGGTTTCGGCTGCGGTTCCTGCGGCA‑GATAACCATTCTTGAGGTCTGGCTGCGGACGCGCTTCACCTTGGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGtttt < 1:328420/249‑1 (MQ=255) ccATCCAGGCGTTACAGGGCGTAAACCGCTTAAGAAACCTCTTCTTCAGGGGCCTCACGCACGGTGTGTCGCGGGTTCAGCTGGGGTTCCTGGGGCA‑GGTAACCAATCTGTATGTCGGGCTGCGGCCGCGCTTCACCTTCGTTGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGttt < 1:367624/248‑1 (MQ=255) tttCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCCATGCCCGCCATAATGCgcagca > 1:517519/1‑164 (MQ=255) ttcggggCGTAAACCACTTAAGAACCCGTTTCTTCAATGGACTCCCGCCGGGTTTGTTCCGGTTTCAGCTGCGTTCCCGGCGGCA‑GGTAACAAATTTTGGTGTCGGGCTGCGGACGCGCTCCCCCTCCGATGTCTTTATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGAc < 1:631452/246‑1 (MQ=255) | GCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGA‑GGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCC‑GCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCA‑GATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGAC > NC_000913/4629991‑4630467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |