Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,132	A→C	G276G (GGT→GGG)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,132	0	A	C	100.0%	101.0 / NA	31	G276G (GGT→GGG)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/8);  total (23/8)

CAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGC  >  NC_000913/6906‑7376                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     cAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGCTAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACCGATGACCCGGGCGTC‑GATAAAAAAGCCAATCATCTGGACAATCCCTTACGCTGCCGGATGCGGAGGCCCGGAAGCCGCTGCCGCTGCCGCGTTTGGCGTCGCCCCCATTACCGCCCCATTGGAAAAc                                                                                                                                                                                                                              >  2:57250/1‑251 (MQ=255)       cAGCACCCCAGGCACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTAGACCCCATTCCCGCCTCATTGGAAAACATACTg                                                                                                                                                                                                                        >  1:14699/1‑251 (MQ=255)                     cccATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGtt                                                                                                                                                                                                          >  1:624936/1‑251 (MQ=255)                       cATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGATGAAAACCGtt                                                                                                                                                                                                          >  1:642856/1‑249 (MQ=255)                         ttaacACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGGTGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGACCAAGACGCCGCTGCCGCTGCCGCGGTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATCCTGCTCTGAAAACCGTTAGt                                                                                                                                                                                                       >  1:197574/5‑250 (MQ=255)                         tgaacACCCGCATCACCTTCTGGAGAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGGAATATCAGCATGGCGCTTCCCTG‑ACAG‑CGCCCCGGGGGTCCGATAAAAATGCCAATCATCTGGACACTCCCTTGCGCTGCCGTATGCGGAGGCCAGGACGCCGCTGCCACTGCCGAGTTTGGCGTCGACCCCCTTCCCGACTCGTTTGAAAACATCCAGCGCTGaacgcacttagg                                                                                                                                                                                                       >  2:543897/5‑240 (MQ=255)                                       gCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTa                                                                                                                                                                                         >  1:676757/1‑250 (MQ=255)                                                                      gCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTcc                                                                                                                                                         >  1:410403/1‑251 (MQ=255)                                                                          gtggttggtcccgtcccccGCCATTAATATCAGCAGGGCGCTTGCCGT‑ACAGATGACCAGGGGGTC‑GATAAAAATGCCAATCATCTGGACAACCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATCCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTgcc                                                                                                                                                     <  1:532575/235‑1 (MQ=255)                                                                                          cccGCCAGTCATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGAACtctc                                                                                                                                     >  2:86498/1‑251 (MQ=255)                                                                                                aGTAAAATCAGCATGGCG‑TTTCCGTTACTGAGGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGAAAATCCCTTGCGCTGCCGGTGGCGGCGGCCGGGACGCCGCTGCCGCTGCCGGGTTTGGCGTCGACCCCTTTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATACCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAAt                                                                                                                               <  1:543897/251‑1 (MQ=255)                                                                                                       tCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCGGCCAGCCAAAAGCACTCTCAAAAATCGACCa                                                                                                                         >  2:167891/1‑250 (MQ=255)                                                                                                           cATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAACCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGa                                                                                                                    <  1:189124/251‑1 (MQ=255)                                                                                                            aTGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCCAAAATAGACCAAATgcc                                                                                                                   >  2:116894/1‑249 (MQ=255)                                                                                                                        gT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGACAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCt                                                                                                                                                        >  1:150143/1‑202 (MQ=255)                                                                                                                            cAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGccc                                                                                                    >  1:191002/1‑251 (MQ=255)                                                                                                                                     ggggTGCC‑GAAAAAAAGGCCAATCATCTGGACAACCCCTTGCGCTGCCGGAGGGGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGCCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCAt                                                                                           <  1:56726/250‑1 (MQ=255)                                                                                                                                      ggggtcgcgaaaaaaaaGCCAATCATCGGGCAAATCCCGTGCGTGGCCGGATGCGGGCGCGAGGACGCGCCGGCCGCTGCCGCGTTTGGCGTCGCCCCAATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTTCCCGATATTCAt                                                                                           <  1:514613/240‑1 (MQ=255)                                                                                                                                                     aTGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAg                                                                                                                                                                                       >  1:576474/1‑144 (MQ=255)                                                                                                                                                        ccAATCATCTGGACAACCCCTGGCGCGGCCGGAGGCGGAGGCCAGGCCGCCGCTGCCGCGGCCGCGTTGGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCt                                                                         <  1:116894/251‑1 (MQ=255)                                                                                                                                                          aaTCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTgg                                                                       >  1:73526/1‑251 (MQ=255)                                                                                                                                                                cTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGt                                                                  >  1:308985/1‑250 (MQ=255)                                                                                                                                                                      aaccccTTGCGCGGCCGGAGGCGGGGGCCAGGCCGCCGCTGCCGCTGCCGCGTTTGGCGTCGCCCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGt                                                                                                         <  1:296579/202‑1 (MQ=255)                                                                                                                                                                      aaTCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGt                                                                                                         >  2:296579/1‑205 (MQ=255)                                                                                                                                                                                gcTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCCGCCAAAAGAACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCCACAGGCGGGTCAGGACCCAGATTACCGCCAt                                                 >  2:591218/1‑251 (MQ=255)                                                                                                                                                                                     cGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAACTGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCCGGCTGGTCAGTACCCAGATTATCGCCATCAACg                                            >  2:526743/1‑251 (MQ=255)                                                                                                                                                                                      ggATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACgg                                           >  1:58691/1‑251 (MQ=255)                                                                                                                                                                                                                          tttGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCt                                                                                                                                                        >  2:204163/1‑106 (MQ=255)                                                                                                                                                                                                                          ttgggCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCt                                                                                                                                                        <  1:204163/103‑1 (MQ=255)                                                                                                                                                                                                                               cGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACcgc  >  1:442586/1‑251 (MQ=255)                                                                                                                                                                                                                               cGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCAAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTGGCCCGATATTCATTACGCAAATAACCAGGCTGGTCAGTACCCAGATTATCGCCATCACCGGGCCAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACcgc  >  2:563648/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGT‑ACAGATGACCAGGGTGTC‑GATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGC  >  NC_000913/6906‑7376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑