Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,281	G→A	I414I (ATC→ATT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,281	0	G	A	100.0%	186.2 / NA	62	I414I (ATC→ATT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/28);  total (34/28)

AACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACG  >  NC_000913/4629054‑4629517                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             aatgcacgtggggCGTGTTCCATCGCGCGCATGGCTTCGATCGCCAGGTCGTTTGTTGGTTCGTCGCGCAGTAGCTTGGTGCCGCCAACCTGCAGCGGCTGTGCCAGATGCAGACTACCGCGCTCTCCACCGGAAATTTGCCCATCGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGCCCAACGCATGCGCGGCGTGGCATCTCGGTGTTGCCGTTCTTCATAATATCCAGCCCGCCTGAAActtc                                                                                                                                                                                                                       <  2:501514/241‑1 (MQ=255)  aCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGCAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGGGTTGCCGGTCTTCATAATATCCAGCCCGCCGGAACcttat                                                                                                                                                                                                                      >  1:602679/1‑249 (MQ=255)       agcagGGCGTTTTCGAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTACCGCCAACCTACAGCAGCTTCGCCACATGCAGACGAACGCGCGCACCACCGGACGGATCACCCACGCGTTTACCCTGATCAACCACTTTAAAGTTACAGCGGACAACGAAGGCGCGGCTTGGCCTCTCGGAGTTGCCGATATACATAATATCCagccagccagcgatttcttacac                                                                                                                                                                                                                 >  2:1498/1‑236 (MQ=255)       agcagGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGACGGTCTTCATAATATCCAGCCCGCCGGAAACTTCTTccc                                                                                                                                                                                                                  >  2:194253/1‑250 (MQ=255)         cagGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGCTCTTCATAATATCAAGCCCGCCGGAACCTTCTTCCCaac                                                                                                                                                                                                               >  2:333453/1‑250 (MQ=255)          agGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTACCAAAc                                                                                                                                                                                                              >  1:181844/1‑251 (MQ=255)            ggcgcgttgctgcgctctcAGGGCTTCCATATCCATGTCGTTGGTTGGTTCGATGAGCCTCACTATGTTGCAGCCACCCTGCCGCAGCTTCGCAAGATGCAGACGACCGCGCTCACCACCGGAAAGCTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAATCGGCCAAAGTAGGCGCGGCTGGGCATCTCGTTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTGCCCAATCgg                                                                                                                                                                                                            <  2:274487/233‑1 (MQ=255)                          cgcgcAGTGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATTTTGCCGCCAACCTGCAGCAGCTTCGCCAGATTCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCTGTGTTGCCGATCTTCATAATAGCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATcc                                                                                                                                                                                              <  1:200670/251‑1 (MQ=255)                                        atatCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGaa                                                                                                                                                                                <  1:668298/251‑1 (MQ=255)                                            ccAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCCACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCACCCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTgt                                                                                                                                                                            >  2:133824/1‑250 (MQ=255)                                            ccAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGCCGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGACCTTCATAATATCCAGCCCGCCGGCAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGACCTg                                                                                                                                                                             >  2:629454/1‑250 (MQ=255)                                             cAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGAt                                                                                                                                                                           <  1:194253/251‑1 (MQ=255)                                               ggTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCTCCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGATTCACGGAACTGATCa                                                                                                                                                                         <  1:237801/251‑1 (MQ=255)                                                      gttgggttcgggcgtaagcagcaTGTTGCCGCTAAGCGGCACCAGCTGCGCTAGAAGCCGACGACCGCGCTCCCCACCGGTAAGTGCACCAACGCGTTTTCCCTGATCAACCCCTTTAAAGTTAAAGCGGCAAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGat                                                                                                                                                                   <  2:373286/234‑2 (MQ=255)                                                                      gggagaattttGCCGGCAACCTGCAGCACCTTCGCCAGATGCAACCGACCGCGCTCACCACCGGAAAGTGCACCACCGCGTTTACCCTGAGCAACCCCTTTAAAGTAAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAgtttcaccgtttc                                                                                                                                                  <  2:182525/242‑1 (MQ=255)                                                                        cagcaTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGCCGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAAGCGCGGATTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCACACGGTTTTGCTGTTATCCATTGAGTCACGGCACTGATCAACCAATGCCAgtttcaccgtttcac                                                                                                                                                >  2:485075/1‑251 (MQ=255)                                                                                       aaCCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTACCAAACGGTTTTGCTGTTATCCATTGAGTAACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCAACGAAAGAGAAGGTGc                                                                                                                                 >  1:511468/1‑251 (MQ=255)                                                                                           cgcatcagctccgccggagggaGACGACCCCGCTCACCACCGGAAAGTTCACCAACGCGTTTCCCCTGATTAACCCCTTTAAAGTTAAAGCGGCCATCGTAGTCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCt                                                                                                                             <  2:127128/230‑1 (MQ=255)                                                                                                                   cgacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGat                                                                                                                                                                   <  1:623813/189‑2 (MQ=255)                                                                                                                   cgacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCAAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGat                                                                                                                                                                   >  2:623813/1‑188 (MQ=255)                                                                                                                             caccacCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGa                                                                                             <  1:265543/249‑1 (MQ=255)                                                                                                                               ccaccGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGCTGGTGCCGCTGTCCGGCTGTTCCTGACCATAGATCTTACgggaacg                                                                                         >  2:16188/1‑246 (MQ=255)                                                                                                                                      aaagatCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGAt                                                                                  >  1:593728/6‑251 (MQ=255)                                                                                                                                       acggtCACCAAAGCGTTTACCCTAATCAACCCCTATAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCACGGGGTTGCCGATCTTCATACAAACCAGCCCGCAGGGAACTTCCTCCCAAAAGGTTATGCTGTTATCCATTGAATACCGGAACTGAACAAACGATGCCAGTTTAACCGTTTCAACTAAAGTGATGGTGCCGGTGTCCGGCTGTACCAGACCCgagaaactactgaaaagagtcgatt                                                                                 >  2:402104/5‑230 (MQ=255)                                                                                                                                       aagTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGAtt                                                                                 >  1:356498/2‑251 (MQ=255)                                                                                                                                                    cggttACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGAt                                                                                                                                                                           <  2:553261/145‑1 (MQ=255)                                                                                                                                                    cgTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGAt                                                                                                                                                                           >  1:553261/1‑148 (MQ=255)                                                                                                                                                            cTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCg                                                             >  1:662351/1‑250 (MQ=255)                                                                                                                                                              gaccAACCCCTTTAAAGTAAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGGTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCgat                                                           <  2:226832/247‑1 (MQ=255)                                                                                                                                                                 cAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCgatgat                                                        <  1:452718/250‑1 (MQ=255)                                                                                                                                                                 cAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCgatgat                                                        <  1:629454/250‑1 (MQ=255)                                                                                                                                                                  aaCCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTCTCCATTGAGTCACGGAACTGATCAACCGa                                                                                                                                                                    >  2:208263/1‑141 (MQ=255)                                                                                                                                                                  aaCCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGa                                                                                                                                                                    <  1:208263/141‑1 (MQ=255)                                                                                                                                                                  aaCCCCTGTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATcc                                                      <  1:51593/251‑1 (MQ=255)                                                                                                                                                                   aCCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATccc                                                     <  1:31440/251‑1 (MQ=255)                                                                                                                                                                    ccccTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCcg                                                    >  1:467768/1‑251 (MQ=255)                                                                                                                                                                     cccTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCcg                                                    >  1:279297/1‑250 (MQ=255)                                                                                                                                                                           aatgtaaaagCGGCCAACGTAGGCTCGACTTGGCATCTCGGTGTTGCCGATCTGCAAAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTGCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGAttg                                             <  2:467301/245‑3 (MQ=255)                                                                                                                                                                                 aaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCtt                                       >  1:89134/1‑251 (MQ=255)                                                                                                                                                                                 aaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCtt                                       >  2:119701/1‑251 (MQ=255)                                                                                                                                                                                     cGGCCAACGTAGGCGCGGCTTTGCCTCTCGGTGGTGCCGATCTTCATAAAATCCAGACCGCCGCAAACTTCTTACCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCAAAAGTTATGGTGCCTCTCGCCGGCTGTTCCTGACCAGAGCACATACGGAACATGGTCGATTTACCCGCGCCGTTCCGCCCGATGCGCCCGACGATTGCCCCtttagg                                   >  2:275930/1‑248 (MQ=255)                                                                                                                                                                                       gCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCCTAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCCTTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCAGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCgggg                                 >  2:172773/1‑250 (MQ=255)                                                                                                                                                                                        ccAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGAt                                <  1:119701/251‑1 (MQ=255)                                                                                                                                                                                        ccAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGAt                                >  1:36672/1‑251 (MQ=255)                                                                                                                                                                                        ccAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGAt                                <  1:485075/251‑1 (MQ=255)                                                                                                                                                                                         cAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCttt                                      >  1:301255/1‑244 (MQ=255)                                                                                                                                                                                         cAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTAGGACCGATGATCCCGACGATTGCCCATTTCGGGATc                               >  1:590713/1‑251 (MQ=255)                                                                                                                                                                                         cAACGCAGGCTCGGCTTGGCAGCTCGGTGTTGCCGATCTTCAAAATATCCTGCCCGCCGGAATCTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCATTTTCACCGTTTCACCGAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCAGACGGAACAGGGGCGATTTACCCGCGCCGTTCGGACCGATGATCCcgacgagtgccccttt                                      <  2:301255/244‑11 (MQ=255)                                                                                                                                                                                          aacggagtcgctgttgggCATCTCGGTGTGTCCGATCTTCATAATATCCAGCCCGTCGGAAACTTCATCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGAGCAACCGATGCCAGTTTCACCGTTTCACCTAAAGGGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCg                              <  2:480117/235‑1 (MQ=255)                                                                                                                                                                                            cGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGCACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGAGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGCCGATTGCCCCTTTCGGGATCg                              >  1:46186/1‑249 (MQ=255)                                                                                                                                                                                             gTAGGCGCGGCTTGGCATCTCGGAGTTGCCGATCTTCATAATATCCAGCCCGCCGGCCACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCCCCGTTTCACATACAGTGAAGGTGACGCTGTCCGGCTGTTCCTGACCAGAGATCATCCGAAACAGGGTAGATTTACCCCCACCGTTCGGACAGATGATCCAGCAGATTGCCCCTTTCGGGAtagcg                            >  2:528456/1‑246 (MQ=255)                                                                                                                                                                                               aggggtggCTGGGCATCTCGGGGTTGCCGATTTTCATAATATCCAGCCCGCCGGAATCTTCTTCCCAAACGGTTTTGCTGTTAGCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGaa                          <  2:467768/244‑1 (MQ=255)                                                                                                                                                                                                  cgcgGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTc                      <  1:116782/251‑1 (MQ=255)                                                                                                                                                                                                    cgGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGCCCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTc                      >  2:417927/1‑249 (MQ=255)                                                                                                                                                                                                     ggCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAgg                   >  1:80962/1‑251 (MQ=255)                                                                                                                                                                                                       cTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc                 >  1:551398/1‑251 (MQ=255)                                                                                                                                                                                                            cATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtcatca             >  1:447302/1‑250 (MQ=255)                                                                                                                                                                                                               ctcGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGGTCCTGACCAGAGATCATACGGAACAGGGTCGATGTACCCGCGCCGTTCg                                                                  <  1:584087/194‑1 (MQ=255)                                                                                                                                                                                                               ctcGGTGTTGCCGATCTTCATAAAATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCCTTGAGTCACGGAACTGATCAACCGTTGCCAGTTTCACCGTTTCACCTAAAGTGATGGGGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGCTTTACCCGCGCCGttag                                                                  >  2:584087/1‑192 (MQ=255)                                                                                                                                                                                                                 cGGTGTTGACGATCTTCATAATATCCAGCCCGCCGGAAACTTCGTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagc       <  2:523401/251‑1 (MQ=255)                                                                                                                                                                                                                     gtTGCCGACCTTCATAATTTCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGGCGCGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagcaggc   <  2:315263/251‑3 (MQ=255)                                                                                                                                                                                                                      ttGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagcaggcg  >  2:252429/1‑248 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             AACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACG  >  NC_000913/4629054‑4629517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑