Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,831	G→A	intergenic (+284/+24)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,831	0	G	A	95.0%	108.9 / ‑2.7	42	intergenic (+284/+24)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (27/11);  total (28/14)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTT  >  NC_000913/4628592‑4629070                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                ggatgatTTGAAACCCTCTCTGGAAGAGGTTGACGAGATAAAAAAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTACGAAATGGCGGATACAAAGGTTGTTGTAGGCCGAAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                                                                                                                      <  1:619549/250‑1 (MQ=255)          gAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAAGGTATTGAAATTTCTTGGTTTT‑TAGGCCGGATAAAGCGTTCACGCCGCATCCGGCATTTTAc                                                                                                                                                                                                                             >  2:461615/1‑251 (MQ=255)               ccTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCAtt                                                                                                                                                                                                                        >  1:274487/1‑251 (MQ=255)                                  cgataatAAACAATGTTTGAAAAAACCGTCACGGGTGCCCCTTTACGAATTTGCGGATACCAAGGCTGTTGTAGCCCGGAAAGGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACACCGTATTGAAATTTCT‑TGGTTTGTAGGCCGTATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGt                                                                                                                                                                                                      <  1:379647/244‑1 (MQ=255)                                    agaTTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACt                                                                                                                                                                                                   >  2:33897/1‑251 (MQ=255)                                           aCAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGAAATACGCTTGTACTTGATAcc                                                                                                                                                                                            >  1:600911/1‑250 (MQ=255)                                                      aaaaaCCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTcc                                                                                                                                                                                  >  1:672002/1‑250 (MQ=255)                                                       aaaaCCAGCAAGGGTGACCCGCTACGAAATTGCGGATACTACGGCTGATGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTAAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCAAGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACACTTCGGCTcc                                                                                                                                                                                  >  2:449584/1‑249 (MQ=255)                                                        aaaCCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAgt                                                                                                                                                                               >  1:533766/1‑250 (MQ=255)                                                           ccTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGtgcg                                                                                                                                                                            >  1:412526/1‑251 (MQ=255)                                                                 cGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCTTTTACGCATTACTTCCCAATACGCTTGTACTTGATAAGCTTCGGCTCCAGTTCGTCTgcc                                                                                                                                                                      >  2:498114/1‑250 (MQ=255)                                                                      gACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTTCGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCTGTGCCCac                                                                                                                                                                 >  2:212432/1‑250 (MQ=255)                                                                      gACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGccc                                                                                                                                                                   >  1:177115/1‑249 (MQ=255)                                                                       acccgataCGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCCCGACGTATCCGGCATTCGGTGCTCAATGCCTGATGCGCCGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTCTTGAAATTTCT‑TGGTTTGTATGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTCCTTCGCAATACGCTTGTACTTTATACGCTTCGGCTCCCGTGCGTCTGCGCCCAgc                                                                                                                                                                >  2:675468/8‑251 (MQ=255)                                                                              aCGAAATTGCGGATACTAAGGCTTTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAgcgtgcg                                                                                                                                                           <  1:33897/249‑1 (MQ=255)                                                                               cgcaaTTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCCGCTCCAGTGCGTCTGCGCCCAGCGTGGGttt                                                                                                                                                        >  2:589034/4‑251 (MQ=255)                                                                                              tAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGt                                                                                                                                          >  1:568799/1‑250 (MQ=255)                                                                                               aaGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGtcc                                                                                                                                        >  2:113171/1‑249 (MQ=255)                                                                                                             ccgaaaaaggcgtTCACGCCGTTTCCGGCATCCGGTGCTAAATGCCTGATGCGACGTTTACCGCGGCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTAcc                                                                                                                          <  2:572039/240‑1 (MQ=255)                                                                                                               ggAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCtt                                                                                                                        >  1:115462/1‑251 (MQ=255)                                                                                                                                    gccGGCATTCTGTGCTCAATGCCTGATGCGACGCTTACCTCGTCTTATCAGGCCTACAGTTTTACAACTTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTAcc                                                                                                     <  2:600911/248‑1 (MQ=255)                                                                                                                                          aTTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGAGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCt                                                                                             >  2:380122/1‑251 (MQ=255)                                                                                                                                            gcGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTg                                                                                            <  1:618194/249‑1 (MQ=255)                                                                                                                                                      aTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAgga                                                                                 <  1:559680/251‑3 (MQ=255)                                                                                                                                                           tGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGc                                                                            >  1:136786/1‑251 (MQ=255)                                                                                                                                                            gATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCg                                                                           >  1:182525/1‑251 (MQ=255)                                                                                                                                                                                ttATCAGGCCTGGAGATTCATAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCATTTTTCGCATTACCTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTAACTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGaa                                                        <  2:177115/250‑1 (MQ=255)                                                                                                                                                                                    caggcctggagattcaCAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAAc                                                   >  2:265543/16‑251 (MQ=255)                                                                                                                                                                                      ggcctggagattcaCAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACgg                                                 >  1:474189/14‑251 (MQ=255)                                                                                                                                                                                           ggagattcaCAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTg                                            >  1:315263/9‑251 (MQ=255)                                                                                                                                                                                                    cAACGTATTGAAATTTCT‑TTGTTTGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTTTACTTGATACGCTTCGGCTCCAg                                                                                                                                                                                <  2:101603/110‑1 (MQ=255)                                                                                                                                                                                                    cAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAg                                                                                                                                                                                >  1:101603/1‑110 (MQ=37)                                                                                                                                                                                                     aaCGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAAcc                                  <  1:652233/251‑1 (MQ=255)                                                                                                                                                                                                        gTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATc                               >  1:581496/1‑251 (MQ=255)                                                                                                                                                                                                                aatttcttggtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCAAGGATGTGCGTGGCGATACGGTCGAGGCACCAACGGTCGTGCGAGATAACCATCGCACAGc                        >  1:501514/11‑251 (MQ=255)                                                                                                                                                                                                                               aGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCcagc          <  2:568799/250‑1 (MQ=255)                                                                                                                                                                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAgg       <  1:178930/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCACCTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGc     >  2:668298/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCg    <  1:461615/251‑1 (MQ=255)                                                                                                                                                                                                                                     gATAAGGCGTTCACGCCGCATCCGGCATTTTAAGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGt   >  1:213924/1‑251 (MQ=255)                                                                                                                                                                                                                                     gATAAGACGTTCAAGCCGCATCCGGCATTTTACGCATTACTTCGAAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTAAGTAAAGTTACCTTCGAAGAACTCAAATTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACAAACGGTCGTGCGAGATACCCATCGCACAGCCCGGGAACTACAGCAgggagt   >  2:382700/1‑248 (MQ=255)                                                                                                                                                                                                                                      acaaGGCGGTCCCGCCGCATCCGGAATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGtt  <  2:533766/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                TGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTT  >  NC_000913/4628592‑4629070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑