Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,898	A→G	L354L (CTT→CTC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,898	0	A	G	100.0%	158.4 / NA	52	L354L (CTT→CTC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/19);  total (33/19)

ACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACC  >  NC_000913/6659‑7134                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 aCCACAGGCGAGAGCAGGAAAATGGCGGACCAATTGGTAATAGCCATGCAGGCCATTATGATATCAGCCAGTTGCCACATAAGCGGAAGACTTAGCAAGGGGCCGCCGATGACCGTTGGGAAGGGGCAGGTCCGCAAAAACCAGATCGCTTTAGGGTTGTTCAGGCGTGAAAAGAAGAGATTGTTTTCGGCGTACATGTAGTTGGCAAA‑GCTGGAGCTGAAGGAAAAAAGCATAAACACGAGGGTAACaa                                                                                                                                                                                                                                        >  2:109751/1‑250 (MQ=255)   cacaGGCGAGATCAGTAAAATGGCGGTTAAAGTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGGTGCCACATCAGCGGAAGACTTAGCAAGGTGTCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTTGTTGGCAAC‑GATGGAGCTGAAGGCAAACATAATAACCACGAGGGTAACAAACt                                                                                                                                                                                                                                     <  2:392602/251‑1 (MQ=255)    acaGGAGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGGCATGCAGGCCAGTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTc                                                                                                                                                                                                                                    <  2:39106/251‑1 (MQ=255)          gagaGCAGTAAAATGGCGGGCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGAGGAAGACTTAGCAAGGGGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACCCCAGATCGCTTTAGGGGTGTTCAGGGGTAAAAAGAAGAGAATGTTTTCGGCGGAAATGTAGTTGGGAAC‑GATGGAGCTGAAGGCAAACAGAAAAACCCCGAGGGGAACAAAATCA‑GCaac                                                                                                                                                                                                                             >  2:474523/1‑249 (MQ=255)             agCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCa                                                                                                                                                                                                                          <  1:306393/251‑1 (MQ=255)               cAGTAAAATGGCGGACAAATTGGTAATAGACATGCAGGCCATTATGATATCTGCCAGTTGCAAAATCAGAGGAAGAATTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCCCAAACACCAGATCGCTTTAGGGATATTCAGGCGTAAAAAGAAAAGCATGTTTTCGGCGCGAATGTAGTTGGCAAA‑GAAGGAGCTGAAAGCAAACAAAATAACCACGAGGGTAACAAAcactgaacaccagg                                                                                                                                                                                                                         >  2:514613/1‑238 (MQ=255)                 gTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGaa                                                                                                                                                                                                                      <  1:585904/251‑1 (MQ=255)                      attgCGGTCAAAGTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTTCCGCCTATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑t                                                                                                                                                                                                                <  1:652653/248‑1 (MQ=255)                         cccgatctatggggaaTAGCCATGCAGGCCATTATGATATCTGCCAGTGGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑aa                                                                                                                                                                                                            <  2:157409/237‑2 (MQ=255)                           ggTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑aaca                                                                                                                                                                                                          <  1:297440/251‑4 (MQ=255)                           ggTCAAATTGGTAATAGCCAAGCAGGCCATTAGGATATCTGCCAGTTGCCACAACAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑aaca                                                                                                                                                                                                          <  2:60162/251‑4 (MQ=255)                               aaaTTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGACGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCg                                                                                                                                                                                                      >  1:472681/1‑251 (MQ=255)                                 aTTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑aa                                                                                                                                                                                                            >  1:157409/1‑242 (MQ=255)                                           gCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGCAGACTTGGCACGGTGCCGCCGGTGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AAAACCCGCATCGCCTTc                                                                                                                                                                                            >  2:532575/1‑249 (MQ=255)                                            ccATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTgg                                                                                                                                                                                         >  1:410889/1‑251 (MQ=255)                                            ccATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTgg                                                                                                                                                                                         <  1:410701/251‑1 (MQ=255)                                            ccATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTgg                                                                                                                                                                                         >  1:556621/1‑251 (MQ=255)                                            ccATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GACCCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTgg                                                                                                                                                                                         >  2:56726/1‑251 (MQ=255)                                             cATGCAGGCCATTATGAGATCTGCCAGGTGCCACATCAGCGGAATACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGa                                                                                                                                                                                        <  2:62207/251‑1 (MQ=255)                                                      cATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGAt                                                                                                                                                                                       <  2:230314/243‑1 (MQ=255)                                                      cATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGAt                                                                                                                                                                                       >  1:230314/1‑243 (MQ=255)                                                       aTTATGATATCTGCCAGATGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGTAGGCAAACAGAATAGCCACGAGGGTAACCAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAAt                                                                                                                                                                              >  2:560600/1‑251 (MQ=255)                                                         tATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATAc                                                                                                                                                                            >  1:183429/1‑251 (MQ=255)                                                                            cacaTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGt                                                                                                                                                        >  1:38219/1‑251 (MQ=255)                                                                            cacaTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGAAGGt                                                                                                                                                        >  1:328806/1‑251 (MQ=255)                                                                               aTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTtgt                                                                                                                                                     >  1:632483/1‑251 (MQ=255)                                                                                  aGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGACAAACAGAATAACCACGCGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGcctggagtgtgtgcc                                                                                                                                                  >  2:289806/1‑237 (MQ=255)                                                                                       aagacTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAATAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCa                                                                                                                                                                                                                               <  1:675854/168‑1 (MQ=255)                                                                                       aagacTTAGCAAGGTGCCGCCGATGACCGTTGAGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCa                                                                                                                                                                                                                               >  2:675854/5‑172 (MQ=255)                                                                                                 taGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGACAACACCAGATAGAATTAGGGTTGGTGAGGCGTGAAAAGAAGAGAATGTTATAGGCGTAAATGTAGTGGGCAAC‑GCAGGAGCTGAAGACAAACAGAAGAACCACGAGGGAAATAAACACA‑GCACCCCAGGAACACA‑GT‑AGCACCACCATCGCCTTCTGGACAAGCTGAATCCCTAC‑CAGCGGCAGGTAGATTGGGCCGTTACCCGCCAgga                                                                                                                                    >  2:265600/2‑248 (MQ=255)                                                                                                     tGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGACTACCTTC‑CAGCGGCATGTGGGTTGTGCCGGTACCCGCCAGTAatacc                                                                                                                               >  2:284433/1‑249 (MQ=255)                                                                                                                    ttGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGcc                                                                                                                >  1:55406/1‑251 (MQ=255)                                                                                                                      gCGAAGTTGCAGTCCCGCAAACCCCAGATTGTTTTGGGGTTGTCCGGGCAAAAAAAAAAGAGTTTGTTTGCGTCTTAAATGGAGTTGGCAAC‑GATGGAGCGGAGGCCAAAAAGAATACCCACGAGGGTAAAAAATTAA‑GCCCCCCAG‑ACCCCA‑TTTAACACCCGCTTCGCTTTTTGGAGAAGCTAATTCCCTTC‑CAGCGGCATTTGGGTTGTGCGGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGt                                                                                                              <  1:316688/251‑1 (MQ=255)                                                                                                                        gAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTAc                                                                                                            >  1:419498/1‑251 (MQ=255)                                                                                                                               cAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGAc                                                                                                     >  1:578512/1‑251 (MQ=255)                                                                                                                               cAGATCCGCAAACACCAGATCGCTTTAGGGATGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAAACCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTCTGCCGTTACCCGCCAGGAATACCCGCATGgccacttccgtccagatgcc                                                                                                     >  2:369636/1‑233 (MQ=255)                                                                                                                                 aacccgaaacctccAGAGCGCTTTGGGTTTTTTCGGGCGTAAAAAGAAGATTTTGTTTGCGGCAAAAATGTGGGTGG‑ACCAGAGGGAGCGGAAGCCAAACATAACACCCGGGAGGGAAACAAACCCA‑CCCCCCCAG‑AACCCAATA‑AACCCCCGCTTCGCCTTCTGGAAAAGCTAAAT‑CCTTCACAGCGGAATGTGGGTTGGCCCTTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCa                                                                                                   <  1:166230/239‑1 (MQ=255)                                                                                                                                      gCAAACACCAGATCTCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCG‑GCACCCCa                                                                                                                                                                                                                          <  1:59949/130‑1 (MQ=255)                                                                                                                                          acacCAGATCGCTTTAGGGTTGTTCCGGCGTAAAAAGAAGAGATTGCTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACAcc                                                                                                                                                                                                        >  2:277168/1‑142 (MQ=255)                                                                                                                                          acacCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACAcc                                                                                                                                                                                                        <  1:277168/142‑1 (MQ=255)                                                                                                                                               aGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCa                                                                                                   >  1:51919/1‑237 (MQ=255)                                                                                                                                                   cgttttAGGGTTGTGAAGGCAAAAAAAAAAGAGATTTTTTTCGGCGAAAATGTATTTGGAAAC‑AATGGACCTGAGGACAAACAAAAAAACCACGAGGTTAACAACCTCA‑CCCCCCCAGGACCCAA‑TA‑ACCCCCCGCATCGCCTTCGGAATAAGCGAAATCCCTCC‑CAGGGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAAt                                                                                   <  1:474523/246‑1 (MQ=255)                                                                                                                                                       ttAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGcc                                                                                                                                                  >  1:494045/1‑182 (MQ=255)                                                                                                                                                       gtAGGGTTGTTCATGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGATCCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGcc                                                                                                                                                  <  2:494045/181‑1 (MQ=255)                                                                                                                                                                       gTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCt                                                             >  1:426086/1‑251 (MQ=255)                                                                                                                                                                            aagaagAGATAGTTTTCGGAGTAAATGTGGTTGGCAAC‑GATGGCGCTGAAGACAAACCGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCAACGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGCAGGCTGTGCAGTTACCCGCCAGTAAAACCAGCAGGGCGCTTTCGGTACAGATGCGCAGGGATTCGGTAAACATGCCACACATATGGCCCATCCCTTgccc                                                        >  2:423325/1‑249 (MQ=255)                                                                                                                                                                                       ttttcgggcgaaaaTGTAGTTGGAAAC‑GATGGAGCTAAAGGAAAACAGAATACCCTCGAGGGTAAAAAAC‑CACGCCCCCCAGAACCCCA‑TT‑AACCCCCGCATCGCCTTCTGGATAAGCTAAATCCCTTC‑CAGCGGTATGTAGGTTGGGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGc                                              <  1:182574/239‑1 (MQ=255)                                                                                                                                                                                                gtAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAgg                                    >  1:108881/2‑251 (MQ=255)                                                                                                                                                                                                                    aTGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAgg                                         >  1:565976/1‑227 (MQ=255)                                                                                                                                                                                                                     tGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGAGCTGCCGGGTGCGGAGGCCAGGACGCCCCTGCCGCTGCcgcg                >  2:573852/1‑251 (MQ=255)                                                                                                                                                                                                                             aaGGCAAACAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGAAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAAACCCTTGAGCTGCCGGATGCGGAGGCCAGGAAGCCGCTGCCGCTGCCGCGTTTGGCgg        >  1:517001/1‑250 (MQ=255)                                                                                                                                                                                                                                   aaCAGAATAACCACGAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCCGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGaccc  >  1:62464/1‑248 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTAGTTGGCAAC‑GATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCA‑GCACCCCAGGAACCCA‑TT‑AGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTC‑CAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACC  >  NC_000913/6659‑7134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑