| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,628,683 | G→A | intergenic (+136/+172) | nadR → / ← ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,628,683 | 0 | G | A | 91.2% | 78.4 / 0.3 | 34 | intergenic (+136/+172) | nadR/ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (16/15); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
ACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGG‑AACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATG‑CTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTT > NC_000913/4628450‑4628920 | acaaaacgaattttGTGGGGGTAGAAGGGGAAGATGAGGACAGCCGTTCCCTGCG‑GGGGTGGGACCTGGTGGGGGAGAGGGGGGGGGAGAAGAGAAAACCGGAAGGAACCTGCCAAAAGGCGAGTTAAAAAATGAGTTTTTTTGAGGATTGGAAAACCTCTTTGAAAGGGGTGGGAGAGATTAAACAGGGTTGAAAAAAACCGGCAGGGGTGACCCGCCACGAAATTGAGGATG‑CTAAGGCTGTTGTAGAc < 2:308665/240‑1 (MQ=255) cgagtatgaaagccGTTTCCTGCGCGGCGGGG‑TGCTGGTGCGGGAGATGAGGGGGGAGCAGAGTTAACCGGGATGAACCGGCTCAAAGGCGAGGTATAAAAAAAGTTTTTT‑GTGGAGTGGAAAACCTCTCTGAAAGGGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGCCCCGCTACGAAAT‑GGGGAAGACTAAGGCTGTTTTGGGCGGCAAAAGGTTTTCACGCCGTATCCGGCATTc < 2:418302/238‑1 (MQ=255) gACAGCCGTTTCCTGCGCTGCGTGG‑AGCTGGTGCGGGAGATGAAGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTGTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTc < 2:141713/251‑1 (MQ=255) ctgcgctgcgTGG‑AGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGCAAAGGCGTTCACGCCGGATCCGGCATTCGGttgatcggaagggcgtg > 2:576366/1‑235 (MQ=255) ctgcgctgcgTGG‑AGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGt < 1:576366/235‑1 (MQ=255) ggTGG‑AGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTa < 1:119534/250‑1 (MQ=255) gggggcgggTGGGGGAGATGATGGGGGGTCAGAGATACCCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTTTCTGGAAGAGGCGGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCGGTGGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTAcc < 1:25684/244‑1 (MQ=255) atgGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCtggag < 1:590957/251‑5 (MQ=255) gCAGAGATACCCGCGAGGAAACGGCTCAAAGGGGAGGTATAAAATAAGTTTGTG‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTGGAAAAAACCTGCACGGGGGACCCGCTACGAAATGGAGGATA‑TTAAGGCTGTTGTAGGCGGGAAAAGGCGTTCACGCCGTATCCGGCAGTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCtggagattcacaa < 2:141775/251‑13 (MQ=255) agaTAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGACTGACGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACACGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTAACAGGCATTCGGTGGCAAAAGCCTGAGGCGACGTCTT‑CCGCGTATTATGAGGCCtggatattcaacacgt > 2:452718/1‑235 (MQ=255) aataccggcgatgaacatgcctaaaGGCGAGGTATAAAATAAGTTTTTT‑GAGGAGTTGAAACCCCCTCGGGAATAGGCTGTCGAGATTAAAAAAGGTTTAAAAAAACCTCCCCGGGTGCCCCGCTACGAAATTGTGGATA‑TTAAGGCCTTTGTGGGCCGGAAAAGGCGTTCACGCCGTATCCGGTATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCATGCCTGGAGATTCACAACGTAt < 2:462493/229‑1 (MQ=255) aaaCCGCGATGAAACGGCTCAAAGGCGAGGTATAAAAT‑AATTTTTTTTATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGCCCCGCTACGAAATTGCGGATA‑CTAAGGCGGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTg < 1:665566/250‑1 (MQ=255) tCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttgt > 1:531736/1‑238 (MQ=255) aaaGGCGTGGTAAAAAATAAGTTTTTT‑GATGAGTTGAAACCCTCTCGGGAAGAGTCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACAAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCTTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGc < 1:128432/251‑1 (MQ=255) ggCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGTTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCgg < 1:412455/251‑1 (MQ=255) tataAAATCAGTTATTT‑GATGAGTTGAAAACCTCTCTGGCAGAGGCTGTCAAGCTTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTACGGCTGATGTAGGCCGGAAAAGCCGTTCACGCCGTATCCGGCATTAGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGCGTCTCACAACTTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGc > 2:679700/1‑251 (MQ=255) aaaaaagaagttttttGAGGGGTTGAAAACCGCTCTGGAAGAGGTGGTCGAGATTAACAAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATGGCGGATA‑TTAAGGCTTTTGTGGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGc < 2:410576/240‑1 (MQ=255) ggatgatTTGAAACCCTCTCTGGAAGAGGTTGACGAGATAAAAAAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTACGAAATGGCGGATA‑CAAAGGTTGTTGTAGGCCGAAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg < 1:619549/250‑1 (MQ=255) gAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAAGGTATTGAAATTTCTTGGTTTT‑TAGGCCGGATAAAGCGTTCACGCCGCATCCGGCATTTTAc > 2:461615/1‑251 (MQ=255) ccTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCAtt > 1:274487/1‑251 (MQ=255) cgataatAAACAATGTTTGAAAAAACCGTCACGGGTGCCCCTTTACGAATTTGCGGATA‑CCAAGGCTGTTGTAGCCCGGAAAGGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACACCGTATTGAAATTTCT‑TGGTTTGTAGGCCGTATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGt < 1:379647/244‑1 (MQ=255) agaTTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACt > 2:33897/1‑251 (MQ=255) aCAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATG‑CTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGAAATACGCTTGTACTTGATAcc > 1:600911/1‑250 (MQ=255) aagttttGAAAAACCCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTc < 1:250383/191‑1 (MQ=255) aaaaaCCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTcc > 1:672002/1‑250 (MQ=255) aaaaCCAGCAAGGGTGACCCGCTACGAAATTGCGGATA‑CTACGGCTGATGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTAAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCAAGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACACTTCGGCTcc > 2:449584/1‑249 (MQ=255) aaaCCTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAgt > 1:533766/1‑250 (MQ=255) ccTGCACGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGtgcg > 1:412526/1‑251 (MQ=255) cGGGTGACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCTTTTACGCATTACTTCCCAATACGCTTGTACTTGATAAGCTTCGGCTCCAGTTCGTCTgcc > 2:498114/1‑250 (MQ=255) gACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTTCGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCTGTGCCCac > 2:212432/1‑250 (MQ=255) gACCCGCTACGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGccc > 1:177115/1‑249 (MQ=255) acccgataCGAAATTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCCCGACGTATCCGGCATTCGGTGCTCAATGCCTGATGCGCCG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTCTTGAAATTTCT‑TGGTTTGTATGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTCCTTCGCAATACGCTTGTACTTTATACGCTTCGGCTCCCGTGCGTCTGCGCCCAgc > 2:675468/8‑251 (MQ=255) aCGAAATTGCGGATA‑CTAAGGCTTTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAgcgtgcg < 1:33897/249‑1 (MQ=255) cgcaaTTGCGGATA‑CTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCT‑TGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCCGCTCCAGTGCGTCTGCGCCCAGCGTGGGttt > 2:589034/4‑251 (MQ=255) | ACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGG‑AACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATG‑CTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACG‑CTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCT‑GGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTT > NC_000913/4628450‑4628920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |