Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,442	C→G	L376L (CTC→CTG)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,442	0	C	G	100.0%	182.4 / NA	56	L376L (CTC→CTG)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (28/28);  total (28/28)

ATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAG  >  NC_000913/4628216‑4628679                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               atGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAAAAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGGGGAGATGCTGGAAGAGGACAGTAACGAAttcggg                                                                                                                                                                                                                        >  1:92608/1‑247 (MQ=255)   gCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGAGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCg                                                                                                                                                                                                                      >  1:462493/1‑251 (MQ=255)   gCCAATAAAGTGACGTTTATTGACACTGATTTTGTCACTACCCAGGAGTTCTGCAAAAAGTACGAAGGGCGTGAGCTTCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACACCACGCCGTGGGCGGAGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGGCGAGAACAATATCTAAttcgagcg                                                                                                                                                                                                                      >  2:287246/1‑245 (MQ=255)    ccAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCAGTTCGTACAGGAGTTGGTTGATGAATAACGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCAGTGGGTGGCGGATGGGTTACGCAGCCTCGGCAGTTCGGTGGAGCGCAAAGAGTTCCCGAACTTGCTGGTGGGGTTGCTGGCAGAGAACAATATCGACTTCGTGCgg                                                                                                                                                                                                                     >  2:25684/1‑251 (MQ=255)         aaatgggcgtttattgactcttaTTTTGTCACTTCCCAGGCGTTCTGAAAAGAGTACGAAGGGCGTGAGCATCGGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGAGCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCTGTTCGGTGGATCGCAAAGAGTTCCAGAGCTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGTTTGa                                                                                                                                                                                                                <  2:600848/229‑1 (MQ=255)                   ttattgacactgATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACACCACGCCGTGGGTGGCGGATGGTTTAAGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGCACAATATCGAATTCGTGCGGGTTGAAGAggtagtt                                                                                                                                                                                                      >  2:128432/12‑246 (MQ=255)                         acactgATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGATGGTAGAGATACTGGAAGAGAACAATATCTAATTCGTGCGGGTTGGAGAggtcgcttatgac                                                                                                                                                                                                >  2:178930/6‑240 (MQ=255)                         acactgATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGAGGAATACCGTTTCGATCTGGTGCTCCTGCTGGAGAACAACACGACGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCGGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTAGTGCGGGTTGACGAGGACGAttctgac                                                                                                                                                                                                >  2:619549/6‑246 (MQ=255)                              gATTTTTTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCCTCCGTTCGTACAGGCGTTGATTGATGAATTCCGTTGCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGATGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAgcc                                                                                                                                                                                            <  2:479716/250‑3 (MQ=255)                                     tcactacccAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGGGATGCTGGAAGAGAAAATTATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCGGTTTCctg                                                                                                                                                                                    >  2:474142/9‑251 (MQ=255)                                     cctctacccAGGCGTTCTGCAAAAAGTGCGTAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCGGCTGGAGAACAACTCGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCTGGTTGAAGAGGACGATTATGACAGCCGTTTCctg                                                                                                                                                                                    <  2:114686/243‑1 (MQ=255)                                       cctccccAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCTTACAGGTGTGGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTGCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCctgcg                                                                                                                                                                                  <  2:379039/245‑1 (MQ=255)                                          ccccAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCctgcgctg                                                                                                                                                                               <  2:659709/248‑1 (MQ=255)                                            ccAGGCGTTCTGCAAACAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATAACGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGGTGGTTTACGCAGCCTCGGCAGTTCGGTGGTTCGCCAAGAGTTCCAGAACTTGCTGGGGGAGATGATGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGCAGCTTATGACAGCAGTTTCctgcgcggcg                                                                                                                                                                             >  2:618194/2‑251 (MQ=255)                                                       gCAAAAAGTACGAAGGGTGTGAGCATCCGTTTGTATAGGCGTGGATGGAATAATCCCGGTTCGATCTGGTGATCCTGCTGGAGAACAACGCGACGTGGGTGGCGGATGGTTGAAGAAGCCCCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGACCAATATCGAACTCGGGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGagcag                                                                                                                                                                     <  2:10807/248‑5 (MQ=255)                                                                        cgtgagcATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGCAGAGGACGATTATGACGGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATgggg                                                                                                                                                   >  2:102940/7‑249 (MQ=255)                                                                        cgtgagcATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATgggg                                                                                                                                                   <  1:102940/243‑1 (MQ=255)                                                                         gtgagcATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATggggg                                                                                                                                                  >  1:410576/6‑249 (MQ=255)                                                                             gcATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGAGGATGGTTTACGCAGCCTCGGCAGTTCGGAGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAag                                                                                                                                                                                                                                            >  1:647028/2‑155 (MQ=255)                                                                             gcATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCGGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAag                                                                                                                                                                                                                                            <  2:647028/154‑1 (MQ=255)                                                                                          cAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGAc                                                                                                                                                                                                <  2:170107/186‑1 (MQ=255)                                                                                          cAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGAc                                                                                                                                                                                                >  1:170107/1‑186 (MQ=255)                                                                                          cAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGcgatg                                                                                                                               <  1:474142/251‑1 (MQ=255)                                                                                            ggCGTTGATTGATGAATCCCTTTTCGATCTGGTGATCCTGCTGGAGATCAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGGTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAAtt                                                                                                                                                                                                                            <  1:499214/156‑1 (MQ=255)                                                                                              ggtttattgagGAAAACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGGGGGTGGCGGATGGTTTACGCAGCTTCGGCATTTCGGTGGTTCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCGGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAAc                                                                                                                           <  2:361816/246‑1 (MQ=255)                                                                                                  gattgacGAATACCGGTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGATCAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCt                                                                                                                       <  2:264780/251‑1 (MQ=255)                                                                                                      gatgaATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCaaa                                                                                                                   <  1:529344/251‑1 (MQ=255)                                                                                                        tgaATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGTTGAAACGGCTCAAAgg                                                                                                                 >  1:295896/1‑251 (MQ=255)                                                                                                        tgaATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGAGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGAAGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGAGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGTTGAAACGGCTCAAAgg                                                                                                                 >  2:559680/1‑251 (MQ=255)                                                                                                          aattctgtgtCGATGTGGTGATCCTGCTGGAGAACAACACCCCGTGGGTGGAGGATGGTCTACGCAGACTCGGCAGTTCGGTGGAGCGCAAAGAGTTCCAGAACGTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACTATTATGACAGCCGTTTCCTGCGCTGCGTGGAGATGGTGCGGGAGATGATGGGGGAGCAGAGATAACTGCGATGAAACGGCTCAAAggag                                                                                                               <  2:92608/242‑3 (MQ=255)                                                                                                          aaTACCGGTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCg                                                                                                               <  2:513682/251‑1 (MQ=255)                                                                                                                gTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGcgatga                                                                                                                              <  1:262519/230‑1 (MQ=255)                                                                                                                gTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGcgatga                                                                                                                              >  2:262519/1‑230 (MQ=255)                                                                                                                 tttCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAagag                                                                                                                                                                                                                                          <  1:344741/121‑1 (MQ=255)                                                                                                                 tttCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAagag                                                                                                                                                                                                                                          >  2:344741/1‑121 (MQ=255)                                                                                                                                   gctgGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGttttttgat                                                                                        <  1:161335/249‑4 (MQ=255)                                                                                                                                     tgGAGAACACCACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGACCTTGCTGGTGGCGATGCGGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGGGATGATGGGGGAGCAGCGATAACCGCGATGAAACGGCTCAACGGCGAGGTATAAAATAAGTATTTT‑GATGAgg                                                                                   >  1:619089/1‑250 (MQ=255)                                                                                                                                        agaACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTGTGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGa                                                                                <  2:183137/251‑1 (MQ=255)                                                                                                                                              acacGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACct                                                                          >  1:8604/1‑251 (MQ=255)                                                                                                                                                              ggATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTACCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGGGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGGTGAAAACCTCTCTGGAAGAGGCTGt                                                          <  2:295896/251‑1 (MQ=255)                                                                                                                                                                       aCGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGACGAGGCTGTCGAGATTa                                                  >  1:141775/1‑250 (MQ=255)                                                                                                                                                                       aCGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTa                                                  >  1:652791/1‑250 (MQ=255)                                                                                                                                                                          cggCCTCGGCAGGTCGTGGGAACGCAAAGAGTGCCAGAAGTTGCTGGAGTAGATGCTGGAAGAGAACAATAGCGAATTCGGGCGGGGTGAAGAGGACGATTATGACAGACGTTTCCTGCGCGGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACa                                              <  2:150004/249‑1 (MQ=255)                                                                                                                                                                            gCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACa                                              >  1:572039/1‑249 (MQ=255)                                                                                                                                                                                        cGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGaaaaaa                                <  1:410826/250‑1 (MQ=255)                                                                                                                                                                                        cGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAAc                               <  1:287246/251‑1 (MQ=255)                                                                                                                                                                                                cGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAACATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACgg                       >  1:663404/1‑251 (MQ=255)                                                                                                                                                                                                  aaaaGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCGCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGt                     <  2:140859/250‑1 (MQ=255)                                                                                                                                                                                                      gagTTCCAGAACTTGCTGGTTGAGATGCTGGAAGAGAACAATATCGAATTCGGGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGGTTTTT‑GATGATTTGAAAACCTCTCTGGAAGAGGCTTTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGAcc                 <  2:613283/251‑1 (MQ=255)                                                                                                                                                                                                        gTTCCAGAACTTGCTGGAGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGACGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGCGCTGGTGCGGGAGATGATGGGGGAGCAGCGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAGGAGGCTGGCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGACCCg               >  2:412455/1‑251 (MQ=255)                                                                                                                                                                                                           acAGAACTTGCTGGAGGAGATGCTGGAAGAGAACAATATCGTATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCGCAAAGGCGAGGTATAAAATAAGTTTTTT‑GCTGAGTTGAAAACCTATCTGGCAGAGGCTGTCGAGATTAAACAAGAGTTT‑AACAAACCTGCACGGGGGACCCGct             >  2:652233/2‑249 (MQ=255)                                                                                                                                                                                                            cAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCTGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGACCCGctac           <  1:308548/251‑4 (MQ=255)                                                                                                                                                                                                               aaCTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCa                                                                                                                     >  2:435094/1‑144 (MQ=255)                                                                                                                                                                                                               aaCTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCa                                                                                                                     <  1:435094/144‑1 (MQ=255)                                                                                                                                                                                                                   tGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTg    >  1:465327/1‑251 (MQ=255)                                                                                                                                                                                                                     cTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTgcg  >  1:11873/1‑249 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               ATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAG‑GTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAG  >  NC_000913/4628216‑4628679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑