Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,986	C→G	T224T (ACC→ACG)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,986	0	C	G	95.3%	131.6 / ‑0.9	43	T224T (ACC→ACG)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (23/18);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGC  >  NC_000913/4627756‑4628231                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTtgt                                                                                                                                                                                                                                   >  1:205443/1‑251 (MQ=255)                    gggATGTCTGGATCAACGGCATCAAAAAGGTTATCACAGTAATGGGGATTCATCCGGA‑CTGGATCTACGCCTCGGAATAGTCCGATTCGCCACAGGATATTGAACATGTGGGTACCGATACGGTGCTGGTCTATCCGAAACGGATCTCTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGGTCCGCTACTGGGAATATTATCCTACGGAAGTGAAGCCGTGCCTTGTACGTACCGCGTCGATccgt                                                                                                                                                                                                                <  2:99347/251‑3 (MQ=255)                           gtgGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTggtgtcg                                                                                                                                                                                                         >  1:111290/1‑246 (MQ=255)                                     ggCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCgg                                                                                                                                                                                               <  2:205443/251‑1 (MQ=255)                                      gCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGt                                                                                                                                                                                              >  1:140523/1‑251 (MQ=255)                                           aaaaaGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATc                                                                                                                                                                                         >  2:210179/1‑251 (MQ=255)                                                               ggggATTCAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTg                                                                                                                                                                     <  1:362649/250‑1 (MQ=255)                                                                    ttcAGCCGTACCTG‑ATCTACACCTCGGCAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGAAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATCTTCCTACGGAAGTGAAGCCGTTCTTTGTACGGACCGTg                                                                                                                                                                                                                         >  2:664094/3‑194 (MQ=255)                                                                    ttcAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTg                                                                                                                                                                                                                         <  1:664094/192‑1 (MQ=255)                                                                    ttcAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAat                                                                                                                                                                >  1:371286/3‑251 (MQ=255)                                                                      cAGCCGGACCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGACGAGCGGTACATCCACCCTGGTAAACAAACTTGCCAatat                                                                                                                                                              >  2:371793/1‑251 (MQ=255)                                                                       catccggacctgaactccactgtggAAGAAGCCGATGCGCCACAGTATATGGACCATCTTGGGATCGATACGGTGCTGGTCGATCCGAAACGTTCCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGGGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCTCCCTGGTAAACAAACTTGCTAata                                                                                                                                                               <  2:142257/227‑1 (MQ=255)                                                                           ggacctg‑atctACACCGTGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCa                                                                                                                                                         <  1:383580/247‑1 (MQ=255)                                                                            gacctg‑atctACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCaa                                                                                                                                                        <  2:140523/248‑1 (MQ=255)                                                                            gacctg‑atctACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCa                                                                                                                                                         >  1:626983/4‑250 (MQ=255)                                                                                      tACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGCTCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATaccacc                                                                                                                                                 >  1:502867/1‑249 (MQ=255)                                                                                          ccTCGGAAGAAGCCGATGCGCCACAGTATATGGACCATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTTCCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATAGCTTCCATACCACCAGTGCg                                                                                                                                           <  2:111290/251‑1 (MQ=255)                                                                                           cTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGt                                                                                                                                          >  1:600848/1‑251 (MQ=255)                                                                                             cGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGc                                                                                                                                                                                                                       >  1:521074/1‑172 (MQ=255)                                                                                             cGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTgg                                                                                                                                        <  1:676904/251‑1 (MQ=255)                                                                                             cGGAAGAAGCCGAGGAGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGc                                                                                                                                                                                                                       <  2:521074/172‑1 (MQ=255)                                                                                                            cgcCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGACTATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGGGCGTGGGAATATGGTcgcgct                                                                                                                         >  2:336285/1‑249 (MQ=255)                                                                                                              ccACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGAtt                                                                                                                        >  1:90310/1‑250 (MQ=255)                                                                                                                acaGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATg                                                                                                                     >  1:19668/1‑251 (MQ=255)                                                                                                                   gTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCt                                                                                                                  >  1:630423/1‑251 (MQ=255)                                                                                                                         ggAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTca                                                                                                             <  1:202391/250‑1 (MQ=255)                                                                                                                          gAACACCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTcac                                                                                                            >  1:181362/1‑250 (MQ=255)                                                                                                                           aaCATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCacac                                                                                                          >  1:588537/1‑251 (MQ=255)                                                                                                                            aCATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGAAAACAAACTTGCCAATATCTTCAATACAACCAGTGCGTGGGAATATGGTCGCGATTATGTATTTTAACAcc                                                                                                         >  2:12817/1‑251 (MQ=255)                                                                                                                                  ggggATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTc                                                                                                       <  1:667311/247‑1 (MQ=255)                                                                                                                                     gATCGATACGGGGCTGGTCGATCCGAATCGTCCCTTTAGGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATAGATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTTGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGt                                                                                                 <  2:76345/250‑1 (MQ=255)                                                                                                                                      aTCGATACGGTGCTGGTCGATCCGAAACGTCCCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGCATATATTCCTCCGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTACATCCACCCTGGAAAACCAACTTGCCAATATCTTCACTACCACCATTGCGTGGGAATCTGGTCGCGCTTATGTCTTTTCACCCCCCGGCGGttg                                                                                               >  2:247766/1‑249 (MQ=255)                                                                                                                                        cgataCGGTGCTGGGCGATACGAAACGTTCCTTTATGAGGATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTAATGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCCCACCTCGGCGGtgat                                                                                              <  2:371286/246‑1 (MQ=255)                                                                                                                                            aCGGTGCTGATCGATCCGAAACGTACCTTTATGAGTATCAGCGGAGAGCAGATGCGCGAAAACCAGATAAGATCCTGGGAATATCTACCTACGGAACTAAAGAAGCTATTTGTACGTACCGTGGCGCTCATTGGTGGCGCGCCGAGCGGTCAATCCACCCCGGGAAACAAACTTGACGATCTCATCAATAACCCCAGTGCGTGGGAATATGGAAGCGATTATATCTTTTCCCCCATCGGCGGtggtgggat                                                                                         >  2:308548/1‑244 (MQ=255)                                                                                                                                               gTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTTTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGc                                                                                      <  1:106815/251‑1 (MQ=255)                                                                                                                                                             gAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTccg                                                                        <  1:12817/251‑3 (MQ=255)                                                                                                                                                                       tttATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATTAGATCGCATTGCAGTATTCCGATTACGATa                                                               >  1:148567/1‑250 (MQ=255)                                                                                                                                                                         tATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTAtttcgcatacgataaa                                                             >  1:10807/1‑236 (MQ=255)                                                                                                                                                                          aTGAGTAGCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGTGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATaaaa                                                            <  1:312347/250‑1 (MQ=255)                                                                                                                                                                                  cAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAAGAGATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACTCCTCGGCGGTGATGAGATCGCATTGGAGTATTCTGACTACGATAAAATCGCGCTgg                                                   <  1:137105/251‑1 (MQ=255)                                                                                                                                                                                      ggTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCa                                               <  2:175525/251‑1 (MQ=255)                                                                                                                                                                                                     gAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACCATACATTgt                                >  1:21863/1‑250 (MQ=255)                                                                                                                                                                                                                                   ccTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCTAGCTGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTAGTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGc  <  1:642891/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTG‑ATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGC  >  NC_000913/4627756‑4628231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑