| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,627,818 | A→G | K168K (AAA→AAG) | nadR → | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,627,818 | 0 | A | G | 91.4% | 93.7 / ‑1.7 | 36 | K168K (AAA→AAG) | nadR | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (17/15); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATC > NC_000913/4627587‑4628048 | tGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGgacctg < 1:629942/250‑1 (MQ=255) aTGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTATATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGATCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGTGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGaagaag < 1:51231/250‑1 (MQ=255) gATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGCAGAAGCCTATGCGCcac > 2:202391/1‑250 (MQ=255) cgcgcaTTGTTTGACGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTCTTCCAAACTTTTACATATCAGCAACATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTATCCGAACGGCTGGGTTGTGTGGAACAACGGCATCACAAAGTTTATGGCTGCAAAAGGGATTCGGCCGGGCCTG‑AT‑AAAACACTCGGAAGAAGCCGATGACCCCCAGTATATggcac > 2:426006/1‑247 (MQ=255) cagcagCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCGAATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATcc > 2:362649/1‑251 (MQ=255) ggATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGa < 1:188639/251‑1 (MQ=255) tcgtctgctctcgttagtacaaccTTTTAAATTTCAGACAAATATTCGCTATCATGCTTTAAACGAAGAGGGAATGGAGCATTATTCGGACGGCTGGGATGGGTGGAGCAACTGCATCAAAAAGTTTATGTCTGTAAAGGGTATTGAGCCGGACGTT‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCCCAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGt < 2:45637/227‑1 (MQ=255) gcgctGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCg > 1:512062/5‑251 (MQ=255) ggTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTgcg < 1:480301/251‑1 (MQ=255) tATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTgcgc > 1:548739/1‑249 (MQ=255) attacAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAg < 1:572529/246‑1 (MQ=255) attacAAACTTTTAAAGATCAGAAAAATAGTCGCTTTCATGATTTCAACGAAGAGGGCATGGAGCCGTTTCCGGTCTGCGGGGATGTGGGGAGCAACGGCAGCAAAAAGTTTATGGTTGAAAAGGATATGCAGCCCGCCCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCGGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAg < 2:672892/246‑1 (MQ=255) cAAACTTTTAAATATCAGAAAAATACTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGAGGTGCGCAGATCcg > 2:667311/1‑251 (MQ=255) cAAACTTTTAAAAATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGCTTATGGATGAAAAGGGGATTCAGACGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTAGCTCCGAAACGTACCTTTATGAGTATAAGCGGTGCGCAGATccc > 2:312347/1‑250 (MQ=255) aaaTATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGAACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGGCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCCGATCCGCGAAAAccc > 1:76345/1‑251 (MQ=255) atatCAGAAAAATATTCGCATTCAGGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGACTGGGATGTGTGGCGCAACGGCATCAAAAAGTCTATGGCTGACAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGGGACACAGTATATGGAACATATGGGGATCGCTACGGTGCTGGTCGAACGTAAACGTACCTTTATGAGTCTAAGGGGTGCGCAGATCCGCGCAAACCCGt > 2:527331/1‑251 (MQ=255) cAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCg < 1:295456/251‑1 (MQ=255) aaaaaTATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCtc > 1:561247/1‑250 (MQ=255) aaaaaTATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAggg > 2:274119/1‑105 (MQ=255) aaaaaTATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAggg < 1:274119/105‑1 (MQ=255) aaaaTAATCGCAGTCATGCTTTCAACGAAGAGGGCATGGATCCGTAGCCGCACGGCTGGGATGTGTGGAGCAACGGCACCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTAc < 1:618578/251‑1 (MQ=255) aaTATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTg > 1:475808/1‑251 (MQ=255) atatTCGCATTCATGCTTTCAACGAAGAGGGGATGGAGCCGTATCCGCACGGCTGGGAGGTGTGGAGCAACGGCATCAGAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACTC‑CTCGGAAGAATCCGATGCGCCACAGTATATTGAACATCTGGGGATCGTTACGGTGCTGGTCGATCCGAAACGTTCCTTTATGAGTAGCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTgg < 1:384599/251‑1 (MQ=255) tatTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTcc < 1:100543/242‑1 (MQ=255) tatTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTggg > 1:175525/1‑251 (MQ=255) tatTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAAAATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCCCAGTTCCGCGAAAACCCGTTcc > 2:100543/1‑242 (MQ=255) gCATTCATGCTTACAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCTTTCCGCTACTGGGAatat < 1:426006/251‑1 (MQ=255) tGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCAGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGAACCTTTATGATTATCAGCCGTGCACAGATCCGCGAAAACCCGGTcctattatggggatataatcct > 2:355115/1‑230 (MQ=255) tCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTAcgga < 1:544492/250‑4 (MQ=255) caaagaggagGGCATGTAGCTGTTTCCGCACGGGTGGGATGTGGTGTGCATCGTCCTCAAAAATGTTATGGCTGAAAATGGGATTCAGACGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGAGCCGCGAAAACCCGTTCCGCGACTGGGAATGTATTccgacggaa < 2:92499/243‑8 (MQ=255) gAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTtgt > 1:205443/1‑251 (MQ=255) gggATGTCTGGATCAACGGCATCAAAAAGGTTATCACAGTAATGGGGATTCATCCGGA‑CTGGATCTACGC‑CTCGGAATAGTCCGATTCGCCACAGGATATTGAACATGTGGGTACCGATACGGTGCTGGTCTATCCGAAACGGATCTCTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGGTCCGCTACTGGGAATATTATCCTACGGAAGTGAAGCCGTGCCTTGTACGTACCGCGTCGATccgt < 2:99347/251‑3 (MQ=255) gtgGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTggtgtcg > 1:111290/1‑246 (MQ=255) ggCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCgg < 2:205443/251‑1 (MQ=255) gCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGt > 1:140523/1‑251 (MQ=255) aaaaaGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATc > 2:210179/1‑251 (MQ=255) | TGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTG‑ATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATC > NC_000913/4627587‑4628048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |