Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,650	T→C	S112S (AGT→AGC)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,650	0	T	C	100.0%	87.6 / NA	29	S112S (AGT→AGC)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

TTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAAC  >  NC_000913/4627407‑4627879                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTACCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGt                                                                                                                                                                                                                                  >  1:624925/1‑251 (MQ=255)      gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCgcag                                                                                                                                                                                                                             <  1:31203/251‑1 (MQ=255)           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGcc                                                                                                                                                                                                                        >  1:142257/1‑251 (MQ=255)                  aTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTg                                                                                                                                                                                                                 <  2:541912/251‑1 (MQ=255)                                   cccAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCgctg                                                                                                                                                                                                <  1:280212/251‑4 (MQ=255)                                     cAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGt                                                                                                                                                                                              >  1:30919/1‑251 (MQ=255)                                        cgtgcAAAAGCTGGAGGCGTTTCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCGTTGGTGTGGTAGGCGGTAAGTTCTACCCGCTGCATACCGGACATATCTGCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATGCCCTCGATCGCGCGTTGGTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTAt                                                                                                                                                                                           <  1:624189/248‑1 (MQ=255)                                                    ggAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACtttt                                                                                                                                                                                >  1:346832/1‑250 (MQ=255)                                                             gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAg                                                                                                                                                                       <  1:89061/250‑1 (MQ=255)                                                              cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGCTACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGa                                                                                                                                                                      >  1:288179/1‑250 (MQ=255)                                                                                             agaagaAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTTCCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAagag                                                                                                                                      <  1:433462/251‑1 (MQ=255)                                                                                                   aaaccattggtttggTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATg                                                                                                                                <  2:30919/237‑1 (MQ=255)                                                                                                             tgtggTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑c                                                                                                                     >  1:60292/5‑251 (MQ=255)                                                                                                                  tATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGATTTGACGATACACGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATCTCAGAAAAATATTCGCATTCATGCTTTCAACGACGAGGGCAAGGAGCAGTAT‑CCGCAc                                                                                                                >  2:676904/1‑251 (MQ=255)                                                                                                                   aTTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACg                                                                                                               <  1:88480/251‑1 (MQ=255)                                                                                                                    ttCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                                                                                                                                   >  1:678857/1‑183 (MQ=255)                                                                                                                    tgcGGTAAGGTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAATACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                                                                                                                                   <  2:678857/181‑1 (MQ=255)                                                                                                                        gTAAGTTCATCCCGCTGCATATCTCACATATCTACCTTATTCAGCGCGCCGGTCGCCAGTGTGACGAACTGCAGATCCTTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCTTGCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTgg                                                                                                          <  2:486964/251‑1 (MQ=255)                                                                                                                                   ccgcTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGCAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGGGTgg                                                                                                 >  2:544492/4‑249 (MQ=255)                                                                                                                                        gcatatctgACATATCTACCTTATCCAGCGCGCCTGTAGCCATGTGGACGAACTGCATAGCATTATGTGTTGTGACGATCCTCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCTCCTGGCCTGATCGTCTGCTCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCATGCATGCTTTCAACGAAGAGGTCATGGAGCC‑TATTCCGTACGGCTGGGATGTGTGGatcatcg                                                                                          <  2:679337/243‑7 (MQ=255)                                                                                                                                          aTACCGGACATATCTACCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGc                                                                                        <  1:146225/251‑1 (MQ=255)                                                                                                                                                   atatCTACCTTATCCGGCGTGCCGGTTGCCACGGTGACGAACTGCATATCATTATGGGTTGTGACGACACACGTGATTGTCCGTTTTTGGAAGACAGCGCTAGTTCGCAGCAGCCCACCGTGCCGGATCTTCTTCTCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATGCATGCTTTCAACGAAGAGGGCATGGAGCCGAAT‑CCGTACGGCTGTGATGTGTGGAGCAACGGCATCaaaaat                                                                               <  2:673933/251‑2 (MQ=255)                                                                                                                                                         aCCTTATCCAGCGCGCCTGGTGCCAGGTTGACGAGCTGCATATCACTATGGGATTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACATCGCTATGTCGCAGCAGCCCACCGTGCCGGAACGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAAGATTCTCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATg                                                                         <  2:288179/251‑1 (MQ=255)                                                                                                                                                         aCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTa                                                                           <  2:60292/249‑1 (MQ=255)                                                                                                                                                                  aGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaa                                                                  >  1:672892/1‑249 (MQ=255)                                                                                                                                                                                     tGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGgacctg                                              <  1:629942/250‑1 (MQ=255)                                                                                                                                                                                                        aTGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTATATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGATCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGTGATTCAGCCGGACCTGATCTACAC‑CTCGGaagaag                          <  1:51231/250‑1 (MQ=255)                                                                                                                                                                                                                    gATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACAC‑CTCGGCAGAAGCCTATGCGCcac              >  2:202391/1‑250 (MQ=255)                                                                                                                                                                                                                                cgcgcaTTGTTTGACGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTCTTCCAAACTTTTACATATCAGCAACATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTAT‑CCGAACGGCTGGGTTGTGTGGAACAACGGCATCACAAAGTTTATGGCTGCAAAAGGGATTCGGCCGGGCCTGAT‑AAAACACTCGGAAGAAGCCGATGACCCCCAGTATATggcac  >  2:426006/1‑247 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        TTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACAC‑CTCGGAAGAAGCCGATGCGCCACAGTATATGGAAC  >  NC_000913/4627407‑4627879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑