Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,629	C→T	D105D (GAC→GAT)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,629	0	C	T	100.0%	100.7 / NA	34	D105D (GAC→GAT)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/19);  total (15/19)

ACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCAC  >  NC_000913/4627396‑4627867                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTATGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGGGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCGACCTTATCCAGCGCGCCTGTAGCCAGGATGACGAAATGCATACCCTTATGGGTTTTGCCGATACACGAGATCGCCCgttggttga                                                                                                                                                                                                                                   >  2:480301/1‑243 (MQ=255)  ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGCGAGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGATTGAAGAc                                                                                                                                                                                                                               >  1:679337/1‑251 (MQ=255)            tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTACCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGt                                                                                                                                                                                                                     >  1:624925/1‑251 (MQ=255)                 gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCgcag                                                                                                                                                                                                                <  1:31203/251‑1 (MQ=255)                  ccAGTTACTGAATGCCAAATTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTTTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGCCATTTCTACCTTATCCAGCGCGCCTTTATCCTGGTTACCGCACTGCATATCCTTATGGGTTTTGCCGATAACCGCGCTCGCGCgttgagatcggtagagggtcgtgttggg                                                                                                                                                                                                                >  2:300197/1‑226 (MQ=255)                  ccAGTTACTGAATGCCAAAGTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCgttg                                                                                                                                                                                                                                        <  1:300197/226‑1 (MQ=255)                      ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGcc                                                                                                                                                                                                           >  1:142257/1‑251 (MQ=255)                             aTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTg                                                                                                                                                                                                    <  2:541912/251‑1 (MQ=255)                                  aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGaa                                                                                                                                                                                                                                  >  1:624783/1‑216 (MQ=255)                                  aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGaa                                                                                                                                                                                                                                  <  2:624783/216‑1 (MQ=255)                                              cccAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCgctg                                                                                                                                                                                   <  1:280212/251‑4 (MQ=255)                                                cAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGt                                                                                                                                                                                 >  1:30919/1‑251 (MQ=255)                                                   cgtgcAAAAGCTGGAGGCGTTTCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCGTTGGTGTGGTAGGCGGTAAGTTCTACCCGCTGCATACCGGACATATCTGCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATGCCCTCGATCGCGCGTTGGTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTAt                                                                                                                                                                              <  1:624189/248‑1 (MQ=255)                                                               ggAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACtttt                                                                                                                                                                   >  1:346832/1‑250 (MQ=255)                                                                        gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAg                                                                                                                                                          <  1:89061/250‑1 (MQ=255)                                                                         cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGCTACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGa                                                                                                                                                         >  1:288179/1‑250 (MQ=255)                                                                                                        agaagaAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTTCCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAagag                                                                                                                         <  1:433462/251‑1 (MQ=255)                                                                                                              aaaccattggtttggTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATg                                                                                                                   <  2:30919/237‑1 (MQ=255)                                                                                                                        tgtggTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑c                                                                                                        >  1:60292/5‑251 (MQ=255)                                                                                                                             tATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGATTTGACGATACACGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATCTCAGAAAAATATTCGCATTCATGCTTTCAACGACGAGGGCAAGGAGCAGTAT‑CCGCAc                                                                                                   >  2:676904/1‑251 (MQ=255)                                                                                                                              aTTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACg                                                                                                  <  1:88480/251‑1 (MQ=255)                                                                                                                               ttCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                                                                                                                      >  1:678857/1‑183 (MQ=255)                                                                                                                               tgcGGTAAGGTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAATACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                                                                                                                      <  2:678857/181‑1 (MQ=255)                                                                                                                                   gTAAGTTCATCCCGCTGCATATCTCACATATCTACCTTATTCAGCGCGCCGGTCGCCAGTGTGACGAACTGCAGATCCTTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCTTGCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTgg                                                                                             <  2:486964/251‑1 (MQ=255)                                                                                                                                              ccgcTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGCAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGGGTgg                                                                                    >  2:544492/4‑249 (MQ=255)                                                                                                                                                   gcatatctgACATATCTACCTTATCCAGCGCGCCTGTAGCCATGTGGACGAACTGCATAGCATTATGTGTTGTGACGATCCTCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCTCCTGGCCTGATCGTCTGCTCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCATGCATGCTTTCAACGAAGAGGTCATGGAGCC‑TATTCCGTACGGCTGGGATGTGTGGatcatcg                                                                             <  2:679337/243‑7 (MQ=255)                                                                                                                                                     aTACCGGACATATCTACCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGc                                                                           <  1:146225/251‑1 (MQ=255)                                                                                                                                                              atatCTACCTTATCCGGCGTGCCGGTTGCCACGGTGACGAACTGCATATCATTATGGGTTGTGACGACACACGTGATTGTCCGTTTTTGGAAGACAGCGCTAGTTCGCAGCAGCCCACCGTGCCGGATCTTCTTCTCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATGCATGCTTTCAACGAAGAGGGCATGGAGCCGAAT‑CCGTACGGCTGTGATGTGTGGAGCAACGGCATCaaaaat                                                                  <  2:673933/251‑2 (MQ=255)                                                                                                                                                                    aCCTTATCCAGCGCGCCTGGTGCCAGGTTGACGAGCTGCATATCACTATGGGATTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACATCGCTATGTCGCAGCAGCCCACCGTGCCGGAACGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAAGATTCTCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATg                                                            <  2:288179/251‑1 (MQ=255)                                                                                                                                                                    aCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTa                                                              <  2:60292/249‑1 (MQ=255)                                                                                                                                                                             aGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaa                                                     >  1:672892/1‑249 (MQ=255)                                                                                                                                                                                                tGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGgacctg                                 <  1:629942/250‑1 (MQ=255)                                                                                                                                                                                                                   aTGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTATATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGATCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGTGATTCAGCCGGACCTGATCTACACCTCGGaagaag              <  1:51231/250‑1 (MQ=255)                                                                                                                                                                                                                               gATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGCAGAAGCCTATGCGCcac  >  2:202391/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                ACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCAC  >  NC_000913/4627396‑4627867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑