Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,627,593 G→A E93E (GAG→GAA)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,5930GA97.3% 111.9 / NA 38E93E (GAG→GAA) nadRDNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (15/21);  minor base C (1/0);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTG  >  NC_000913/4627347‑4627836
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
cAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCATTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCTCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGc                                                                                                                                                                                                                                                  <  1:625246/251‑1 (MQ=255)
 aaGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAACCCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCa                                                                                                                                                                                                                                                 <  2:227851/251‑1 (MQ=255)
                     aCAGCAGTTGGCTGATGGCAGCTGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCGTCGCGGAAAAGCTGGATGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATGCCTGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGttgggacg                                                                                                                                                                                                                             <  2:545516/251‑7 (MQ=255)
                            gtggCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCg                                                                                                                                                                                                                      >  1:45637/4‑251 (MQ=255)
                                  gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCgcgatc                                                                                                                                                                                                                 <  1:163077/250‑6 (MQ=255)
                                   aTGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAACATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAACCCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGACCTGCATCTCATTATGGGTTTTGACGTTACCCgcgatcgc                                                                                                                                                                                                               >  2:88480/1‑244 (MQ=255)
                                                 aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTATGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGGGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCGACCTTATCCAGCGCGCCTGTAGCCAGGATGACGAAATGCATACCCTTATGGGTTTTGCCGATACACGAGATCGCCCgttggttga                                                                                                                                                                                                    >  2:480301/1‑243 (MQ=255)
                                                  ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGCGAGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGATTGAAGAc                                                                                                                                                                                                >  1:679337/1‑251 (MQ=255)
                                                            tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTACCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGt                                                                                                                                                                                      >  1:624925/1‑251 (MQ=255)
                                                                 gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCgcag                                                                                                                                                                                 <  1:31203/251‑1 (MQ=255)
                                                                  ccAGTTACTGAATGCCAAATTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTTTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGCCATTTCTACCTTATCCAGCGCGCCTTTATCCTGGTTACCGCACTGCATATCCTTATGGGTTTTGCCGATAACCGCGCTCGCGCgttgagatcggtagagggtcgtgttggg                                                                                                                                                                                 >  2:300197/1‑226 (MQ=255)
                                                                  ccAGTTACTGAATGCCAAAGTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCgttg                                                                                                                                                                                                         <  1:300197/226‑1 (MQ=255)
                                                                      ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGcc                                                                                                                                                                            >  1:142257/1‑251 (MQ=255)
                                                                             aTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTg                                                                                                                                                                     <  2:541912/251‑1 (MQ=255)
                                                                                  aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGaa                                                                                                                                                                                                   <  2:624783/216‑1 (MQ=255)
                                                                                  aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGaa                                                                                                                                                                                                   >  1:624783/1‑216 (MQ=255)
                                                                                              cccAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCgctg                                                                                                                                                    <  1:280212/251‑4 (MQ=255)
                                                                                                cAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGt                                                                                                                                                  >  1:30919/1‑251 (MQ=255)
                                                                                                   cgtgcAAAAGCTGGAGGCGTTTCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCGTTGGTGTGGTAGGCGGTAAGTTCTACCCGCTGCATACCGGACATATCTGCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATGCCCTCGATCGCGCGTTGGTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTAt                                                                                                                                               <  1:624189/248‑1 (MQ=255)
                                                                                                               ggAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACtttt                                                                                                                                    >  1:346832/1‑250 (MQ=255)
                                                                                                                        gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAg                                                                                                                           <  1:89061/250‑1 (MQ=255)
                                                                                                                         cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGCTACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGa                                                                                                                          >  1:288179/1‑250 (MQ=255)
                                                                                                                                                        agaagaAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTTCCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAagag                                                                                          <  1:433462/251‑1 (MQ=255)
                                                                                                                                                              aaaccattggtttggTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATg                                                                                    <  2:30919/237‑1 (MQ=255)
                                                                                                                                                                        tgtggTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑c                                                                         >  1:60292/5‑251 (MQ=255)
                                                                                                                                                                             tATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGATTTGACGATACACGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATCTCAGAAAAATATTCGCATTCATGCTTTCAACGACGAGGGCAAGGAGCAGTAT‑CCGCAc                                                                    >  2:676904/1‑251 (MQ=255)
                                                                                                                                                                              aTTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACg                                                                   <  1:88480/251‑1 (MQ=255)
                                                                                                                                                                               ttCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                                                                                       >  1:678857/1‑183 (MQ=255)
                                                                                                                                                                               tgcGGTAAGGTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAATACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                                                                                       <  2:678857/181‑1 (MQ=255)
                                                                                                                                                                                   gTAAGTTCATCCCGCTGCATATCTCACATATCTACCTTATTCAGCGCGCCGGTCGCCAGTGTGACGAACTGCAGATCCTTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCTTGCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTgg                                                              <  2:486964/251‑1 (MQ=255)
                                                                                                                                                                                              ccgcTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGCAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGGGTgg                                                     >  2:544492/4‑249 (MQ=255)
                                                                                                                                                                                                   gcatatctgACATATCTACCTTATCCAGCGCGCCTGTAGCCATGTGGACGAACTGCATAGCATTATGTGTTGTGACGATCCTCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCTCCTGGCCTGATCGTCTGCTCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCATGCATGCTTTCAACGAAGAGGTCATGGAGCC‑TATTCCGTACGGCTGGGATGTGTGGatcatcg                                              <  2:679337/243‑7 (MQ=255)
                                                                                                                                                                                                     aTACCGGACATATCTACCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGc                                            <  1:146225/251‑1 (MQ=255)
                                                                                                                                                                                                              atatCTACCTTATCCGGCGTGCCGGTTGCCACGGTGACGAACTGCATATCATTATGGGTTGTGACGACACACGTGATTGTCCGTTTTTGGAAGACAGCGCTAGTTCGCAGCAGCCCACCGTGCCGGATCTTCTTCTCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATGCATGCTTTCAACGAAGAGGGCATGGAGCCGAAT‑CCGTACGGCTGTGATGTGTGGAGCAACGGCATCaaaaat                                   <  2:673933/251‑2 (MQ=255)
                                                                                                                                                                                                                    aCCTTATCCAGCGCGCCTGGTGCCAGGTTGACGAGCTGCATATCACTATGGGATTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACATCGCTATGTCGCAGCAGCCCACCGTGCCGGAACGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAAGATTCTCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATg                             <  2:288179/251‑1 (MQ=255)
                                                                                                                                                                                                                    aCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTa                               <  2:60292/249‑1 (MQ=255)
                                                                                                                                                                                                                             aGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaa                      >  1:672892/1‑249 (MQ=255)
                                                                                                                                                                                                                                                tGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGgacctg  <  1:629942/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
CAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAT‑CCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTG  >  NC_000913/4627347‑4627836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑