Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,627,539 A→G P75P (CCA→CCG)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,5390AG97.4% 109.8 / NA 39P75P (CCA→CCG) nadRDNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (19/19);  minor base T (0/1);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGG  >  NC_000913/4627304‑4627776
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
cGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACa‑t                                                                                                                                                                                                                                >  1:92499/1‑251 (MQ=255)
         atatGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATTCCGGACA‑TATCTACCtt                                                                                                                                                                                                                       <  1:650494/251‑1 (MQ=255)
           ttGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCGACAGGGCTGCACGCTACAGCAGGTGGCTGATGCAAGCGGTATGACCAACGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCAAGCGCGCAAAAGCTGGCGGCGGTGCAACGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAACAAACCATTGGTGTGGGATTCGTTAAGTTCTACCCGCTGCATAACGGACG‑TATCTACCtttt                                                                                                                                                                                                                     >  2:295456/2‑249 (MQ=255)
           atGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCGCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGTTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTAccgta                                                                                                                                                                                                                      <  2:622946/250‑4 (MQ=255)
                        aTTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGTAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTGCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTGCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGt                                                                                                                                                                                                        <  2:303629/251‑1 (MQ=255)
                          tACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTATACACGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGt                                                                                                                                                                                                        >  2:188639/1‑249 (MQ=255)
                             cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGccc                                                                                                                                                                                                   >  2:31203/1‑250 (MQ=255)
                                           cAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCATTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCTCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGc                                                                                                                                                                                     <  1:625246/251‑1 (MQ=255)
                                            aaGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAACCCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCa                                                                                                                                                                                    <  2:227851/251‑1 (MQ=255)
                                            aaGCAACAGGGCTGCACGCTACAGCAGGTGGCTGAGGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACgtactgca                                                                                                                                                                                    <  2:415020/251‑8 (MQ=255)
                                                     ggCTGAACGCTACAGCAGGTGACTGGTGCCAACGGTATAACCAAAGGGTATTTACGACAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCAGAAGGCGTTGCACCGTTTTTTGGGACTTAAGTTTCCCCGGCAGGATAAAACAATTGGTGTGGTATTCGGTAAGTTTTCCCCGCTGCCTACCTG‑CACTATCTACCTTATCCAGCGCTCCTGTAGcccgggggacgaaacgccaatcattat                                                                                                                                                                           >  2:383580/1‑226 (MQ=255)
                                                                aCAGCAGTTGGCTGATGGCAGCTGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCGTCGCGGAAAAGCTGGATGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATGCCTGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGttgggacg                                                                                                                                                                <  2:545516/251‑7 (MQ=255)
                                                                       gtggCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCg                                                                                                                                                         >  1:45637/4‑251 (MQ=255)
                                                                             gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCgcgatc                                                                                                                                                    <  1:163077/250‑6 (MQ=255)
                                                                              aTGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAACATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAACCCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGACCTGCATCTCATTATGGGTTTTGACGTTACCCgcgatcgc                                                                                                                                                  >  2:88480/1‑244 (MQ=255)
                                                                                            aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTATGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGGGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCGACCTTATCCAGCGCGCCTGTAGCCAGGATGACGAAATGCATACCCTTATGGGTTTTGCCGATACACGAGATCGCCCgttggttga                                                                                                                                       >  2:480301/1‑243 (MQ=255)
                                                                                             ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGCGAGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGATTGAAGAc                                                                                                                                   >  1:679337/1‑251 (MQ=255)
                                                                                                       tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTACCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGt                                                                                                                         >  1:624925/1‑251 (MQ=255)
                                                                                                            gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCgcag                                                                                                                    <  1:31203/251‑1 (MQ=255)
                                                                                                             ccAGTTACTGAATGCCAAATTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTTTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGCCA‑TTTCTACCTTATCCAGCGCGCCTTTATCCTGGTTACCGCACTGCATATCCTTATGGGTTTTGCCGATAACCGCGCTCGCGCgttgagatcggtagagggtcgtgttggg                                                                                                                    >  2:300197/1‑226 (MQ=255)
                                                                                                             ccAGTTACTGAATGCCAAAGTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCgttg                                                                                                                                            <  1:300197/226‑1 (MQ=255)
                                                                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGcc                                                                                                               >  1:142257/1‑251 (MQ=255)
                                                                                                                        aTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTg                                                                                                        <  2:541912/251‑1 (MQ=255)
                                                                                                                             aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGaa                                                                                                                                      >  1:624783/1‑216 (MQ=255)
                                                                                                                             aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGaa                                                                                                                                      <  2:624783/216‑1 (MQ=255)
                                                                                                                                         cccAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCgctg                                                                                       <  1:280212/251‑4 (MQ=255)
                                                                                                                                           cAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGt                                                                                     >  1:30919/1‑251 (MQ=255)
                                                                                                                                              cgtgcAAAAGCTGGAGGCGTTTCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCGTTGGTGTGGTAGGCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTGCCTTAGCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATGCCCTCGATCGCGCGTTGGTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTAt                                                                                  <  1:624189/248‑1 (MQ=255)
                                                                                                                                                          ggAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACtttt                                                                       >  1:346832/1‑250 (MQ=255)
                                                                                                                                                                   gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAg                                                              <  1:89061/250‑1 (MQ=255)
                                                                                                                                                                    cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGCTACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGa                                                             >  1:288179/1‑250 (MQ=255)
                                                                                                                                                                                                   agaagaAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTTCCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCTCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAagag                             <  1:433462/251‑1 (MQ=255)
                                                                                                                                                                                                         aaaccattggtttggTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATg                       <  2:30919/237‑1 (MQ=255)
                                                                                                                                                                                                                   tgtggTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATc             >  1:60292/5‑251 (MQ=255)
                                                                                                                                                                                                                        tATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGATTTGACGATACACGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATCTCAGAAAAATATTCGCATTCATGCTTTCAACGACGAGGGCAAGGAGCAGTATCCGCAc        >  2:676904/1‑251 (MQ=255)
                                                                                                                                                                                                                         aTTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACg       <  1:88480/251‑1 (MQ=255)
                                                                                                                                                                                                                          ttCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                          >  1:678857/1‑183 (MQ=255)
                                                                                                                                                                                                                          tgcGGTAAGGTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAATACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACt                                                                          <  2:678857/181‑1 (MQ=255)
                                                                                                                                                                                                                              gTAAGTTCATCCCGCTGCATATCTCACA‑TATCTACCTTATTCAGCGCGCCGGTCGCCAGTGTGACGAACTGCAGATCCTTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAATCTTTTAAATATCAGAAAAATATTCGCTTGCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTgg  <  2:486964/251‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
CGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGG  >  NC_000913/4627304‑4627776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑