Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,377	A→G	V21V (GTA→GTG)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,377	0	A	G	93.2%	172.1 / 2.2	61	V21V (GTA→GTG)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (20/35);  total (22/39)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01

AGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACG  >  NC_000913/4627142‑4627618                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    aGCGGTCAGGAACGAATGTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑Gt                                                                                                                                                                                                                                     <  1:499498/251‑1 (MQ=255)     gTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATg                                                                                                                                                                                                                                  >  1:227851/1‑250 (MQ=255)         ggAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCaa                                                                                                                                                                                                                             <  1:465289/251‑1 (MQ=255)              gaattccagaattggcgAAACACGGTTTGCGTCGGGCGATTGGGCCGGCGGCGAAGGTACCGAAAAAAGCGCCGGAAAGTATTCAGAGTTTTGGCTTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCTACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCTCGCTACAGCAGGTGGCTGATGCCAGCG‑TTATGACCAAAGGGt                                                                                                                                                                                                                        <  2:614445/238‑1 (MQ=255)                  ttcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTAtt                                                                                                                                                                                                                     <  2:636584/246‑1 (MQ=255)                   tcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTAttt                                                                                                                                                                                                                    <  1:516594/247‑1 (MQ=255)                    cagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTaa                                                                                                                                                                                                                  <  1:232563/249‑1 (MQ=255)                       aaGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGcc                                                                                                                                                                                                               <  1:3872/251‑1 (MQ=255)                            gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCAGGACGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGtt                                                                                                                                                                                                           >  1:92382/1‑250 (MQ=255)                             cgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCTCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGTGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCGATAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTAc                                                                                                                                                                                                         <  1:435742/251‑1 (MQ=255)                                  cacGGTGTTCGCCGGGCGATTGTTCCGGCTGCTAACGTACCGAAAATAGCGCCTGAAGTGATGCAGATTTTTGTCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGTAATACGGAGGGTGATATGTCGTCATTTGATTACCTGAAAACTGCCATCATGCATCATGGCGGCACGCTATAGCGTGTTGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAAt                                                                                                                                                                                                    <  1:635613/251‑1 (MQ=255)                                   acGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATg                                                                                                                                                                                                   <  1:313528/251‑1 (MQ=255)                                           cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGCCTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCAAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATACCAAAATc                                                                                                                                                                                           >  1:364779/1‑251 (MQ=255)                                           cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt                                                                                                                                                                                            <  1:675795/250‑1 (MQ=255)                                            gcagggCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCTGAAGTGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTAGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa                                                                                                                                                                                          <  1:357393/248‑1 (MQ=255)                                               gggCGATTGTGCACGCGGCTAACGTACAGAAAAAAGCGCCGGAAGGGATGGAGGTTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCTACGACTTATAATGAGGAATCCGGAGGGAGATATGGCGTCATTTGATTACCTGAAAACTGCCATCAAGCCACAGGGCTGCACTCTACAGCAGGTGACTACTGCCA‑CGTGGATGACCCAAAGGTATTTACGCCAGTTACTGACTGCCCAAATccaa                                                                                                                                                                                        >  2:89061/1‑247 (MQ=255)                                                ggCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAg                                                                                                                                                                                      <  1:446311/251‑1 (MQ=255)                                                    aTTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                                                                                                                                                                                  >  1:593209/1‑251 (MQ=255)                                                      tGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                                                                                                                                                                                  >  2:36288/1‑249 (MQ=255)                                                         tCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATCTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg                                                                                                                                                                              <  2:593209/250‑1 (MQ=255)                                                                   aaCGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg                                                                                                                                                                   <  1:31547/251‑1 (MQ=255)                                                                            aaaaaaGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTg                                                                                                                                                          <  1:4445/251‑1 (MQ=255)                                                                                   cgcCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGtt                                                                                                                                                   >  1:99347/1‑251 (MQ=255)                                                                                    gcCGGAAGGGAGGCAGATTTTTGGCGGTATAAAACTCTCCGACGCGCTTAGCGTGGTCGTCGACTTATAATGAGGAATACGGAGGTAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGTTGCACGCTACAGCAGGTGTCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTTGAGTCGTTGCACCGttt                                                                                                                                                  <  2:521929/251‑1 (MQ=255)                                                                                      cGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGttttt                                                                                                                                                <  1:454887/251‑1 (MQ=255)                                                                                         aaGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTgg                                                                                                                                             <  1:36288/251‑1 (MQ=255)                                                                                              aTGCAGATTTTTGGCGATAAAACACTCTCCGACGCGCATAGCGTGTTCGACGACTTATAATGAGGAAAACGGAGGGAGATATGTCGTCCTTTGATTACATGAAAACTGCCGTCAAGCAACAGGGCTGCACGCTACAGCAGGTGGATGCTACCCGCG‑GTCTGACCACAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCCGCGCGCAAAAGCTGGAGGCGGTGCACCGTTTTTTGAGGCtt                                                                                                                                        >  2:650494/1‑251 (MQ=255)                                                                                                         gtgCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTcccc                                                                                                                              <  2:576170/248‑1 (MQ=255)                                                                                                          ggCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCg                                                                                                                             <  1:391308/250‑1 (MQ=255)                                                                                                                      ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc                                                                                                                                                                     >  1:215848/1‑198 (MQ=255)                                                                                                                      ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc                                                                                                                                                                     <  2:215848/198‑1 (MQ=255)                                                                                                                            gACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAAccattggt                                                                                                          <  1:108868/251‑8 (MQ=255)                                                                                                                                  cTTAGCATGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTACACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAACGGGTATTTAAGCCAGTTACTGACTGCCAAAATCAAAAGCCCCAGCGCGCAAACGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTACCAGGAAGAAGAAAACAATTGgtgtggtt                                                                                                    >  2:572529/1‑247 (MQ=255)                                                                                                                                        gtgtTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGACTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGATGGCGGCGTTGCCCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGt                                                                                              >  2:676740/1‑251 (MQ=255)                                                                                                                                                     tataATGAGGAATACGGAGGGAGGTATGTCGTCATTTGATTACCTGAAAACGGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGTCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCTCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAATTTCTcccc                                                                                   <  2:149109/249‑1 (MQ=255)                                                                                                                                                     tataATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcccg                                                                                  <  1:676740/250‑2 (MQ=255)                                                                                                                                                            aggaataCGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCg                                                                                                                                                     <  1:39008/172‑1 (MQ=255)                                                                                                                                                            aggaataCGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCg                                                                                                                                                     >  2:39008/5‑176 (MQ=255)                                                                                                                                                             gaTATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTTCACGCTACAGCAGGTAGCTTATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGa                                                                                                                                      <  2:1328/190‑1 (MQ=255)                                                                                                                                                             gaTATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGa                                                                                                                                      >  1:1328/1‑190 (MQ=255)                                                                                                                                                                   cGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACa‑t                                                                  >  1:92499/1‑251 (MQ=255)                                                                                                                                                                       gggagATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTa                                                                                             <  2:314108/221‑1 (MQ=255)                                                                                                                                                                       gggagATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTa                                                                                             >  1:314108/1‑221 (MQ=255)                                                                                                                                                                            atatGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATTCCGGACA‑TATCTACCtt                                                         <  1:650494/251‑1 (MQ=255)                                                                                                                                                                             tatGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGGGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcccgct                                                                                >  2:538981/1‑225 (MQ=255)                                                                                                                                                                             tatGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcccgct                                                                                <  1:538981/228‑4 (MQ=255)                                                                                                                                                                              ttGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCGACAGGGCTGCACGCTACAGCAGGTGGCTGATGCAAGCG‑GTATGACCAACGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCAAGCGCGCAAAAGCTGGCGGCGGTGCAACGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAACAAACCATTGGTGTGGGATTCGTTAAGTTCTACCCGCTGCATAACGGACG‑TATCTACCtttt                                                       >  2:295456/2‑249 (MQ=255)                                                                                                                                                                              atGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCGCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGTTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTAccgta                                                        <  2:622946/250‑4 (MQ=255)                                                                                                                                                                                  cgtcATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                                                                                                                                                                                  <  2:91973/125‑1 (MQ=255)                                                                                                                                                                                  cgtcATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                                                                                                                                                                                  >  1:91973/1‑125 (MQ=255)                                                                                                                                                                                           aTTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGTAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTGCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTGCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGt                                          <  2:303629/251‑1 (MQ=255)                                                                                                                                                                                             tACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTATACACGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGt                                          >  2:188639/1‑249 (MQ=255)                                                                                                                                                                                                cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGccc                                     >  2:31203/1‑250 (MQ=255)                                                                                                                                                                                                      aCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgc                                                                                                                                                                               <  1:560746/108‑1 (MQ=255)                                                                                                                                                                                                      aCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGAACAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgc                                                                                                                                                                               >  2:560746/1‑108 (MQ=255)                                                                                                                                                                                                              cAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCATTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCTCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGc                       <  1:625246/251‑1 (MQ=255)                                                                                                                                                                                                               aaGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAACCCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCa                      <  2:227851/251‑1 (MQ=255)                                                                                                                                                                                                               aaGCAACAGGGCTGCACGCTACAGCAGGTGGCTGAGGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACgtactgca                      <  2:415020/251‑8 (MQ=255)                                                                                                                                                                                                               aaGCAACAGGGCTGCACGCTACAGCAGGGTGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGgtgt                                                                                                        <  2:339256/170‑1 (MQ=255)                                                                                                                                                                                                                        ggCTGAACGCTACAGCAGGTGACTGGTGCCAACG‑GTATAACCAAAGGGTATTTACGACAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCAGAAGGCGTTGCACCGTTTTTTGGGACTTAAGTTTCCCCGGCAGGATAAAACAATTGGTGTGGTATTCGGTAAGTTTTCCCCGCTGCCTACCTG‑CACTATCTACCTTATCCAGCGCTCCTGTAGcccgggggacgaaacgccaatcattat             >  2:383580/1‑226 (MQ=255)                                                                                                                                                                                                                                   aCAGCAGTTGGCTGATGGCAGCT‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCGTCGCGGAAAAGCTGGATGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATGCCTGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGttgggacg  <  2:545516/251‑7 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    AGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACA‑TATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACG  >  NC_000913/4627142‑4627618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑