Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,213	G→A	V434V (GTG→GTA)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,213	0	G	A	100.0%	195.0 / NA	60	V434V (GTG→GTA)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/36);  total (24/36)

GCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTG  >  NC_000913/4626971‑4627458                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGAGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGCACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTACAGTACCGaaaa                                                                                                                                                                                                                                                 >  2:491896/1‑250 (MQ=255)       cAGGATGTGTTGTTGAAAGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGTCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGGGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAgcgc                                                                                                                                                                                                                                           <  1:528094/250‑1 (MQ=255)             gtgtTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAgg                                                                                                                                                                                                                                    <  1:236611/251‑1 (MQ=255)                  tgtgAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATg                                                                                                                                                                                                                                <  1:491896/250‑1 (MQ=255)                   gtgAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAACAAGCGCCGGAAGGGATGc                                                                                                                                                                                                                               >  2:232563/1‑250 (MQ=255)                                       gAAGGGAACCGAAACCAGCCCCGCCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGTCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAAc                                                                                                                                                                                                          <  2:83922/251‑1 (MQ=255)                                        aaGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAAct                                                                                                                                                                                                         >  1:149109/1‑251 (MQ=255)                                             aaCCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTcc                                                                                                                                                                                                     <  1:285628/250‑1 (MQ=255)                                             aaCCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCAGGCGGCTAACGTAACGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTCAAAAACTCTCCg                                                                                                                                                                                                    >  1:73171/1‑251 (MQ=255)                                                     ccAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCtt                                                                                                                                                                                            >  1:576170/1‑251 (MQ=255)                                                     ccAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCtt                                                                                                                                                                                            <  1:523349/251‑1 (MQ=255)                                                      cagcgCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTa                                                                                                                                                                                           <  1:446095/247‑1 (MQ=255)                                                       agcgCCGACTTAGCGTTACTGCTGGAGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGGTCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGGTTGTTCCGGCGGCTAACGTACCGAAAAAAGCGACGGAAGGGATGAAGCATTTTGGCGTTAAAAAGctcctcaccccgctt                                                                                                                                                                                            >  2:163077/4‑237 (MQ=255)                                                             gACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGGGtt                                                                                                                                                                                    >  1:166709/1‑251 (MQ=255)                                                             gACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACAAGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAGAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAAGCGCTTAGCGTGtt                                                                                                                                                                                    >  1:521929/1‑251 (MQ=255)                                                              agttAGCGTTACTGCTGGCGAGGGTTTCCAGCCTGCGTGACAGACCGCGGCCACAGGTGCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCGCAGCGGTCAGGGACGAAGTGCAGAAGCGTCGAAACACGGTTTTCGCCGGGCGATGGTTCCGGTGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTGTGGCGTTAAAAAACTCTCCGACGCGGGTAGTGTGTTc                                                                                                                                                                                   <  2:79731/249‑1 (MQ=255)                                                                   ggtgtACTGCTGGCGGGGGTTTCCAGCCTGCGTGACAGATCGCTGCCACAGTATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCTCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTGTGCGTGTTcgacg                                                                                                                                                                               <  2:290367/246‑1 (MQ=255)                                                                      ttACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACtt                                                                                                                                                                           >  1:303629/1‑251 (MQ=255)                                                                           gctgggtttGGTTTCCAGCCTTCGTGAAAGATCTCGGCCACAGGATCTGGGGGTGTTTGGGGAAGTCGGGCTGGCAGTGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATGTCAGAAGCGGCGAAACACGGGTTTCGCCTGGCGATTGTTCCGGTGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGTCGCGCTTAGCGTGTTCGACGATTTATAAt                                                                                                                                                                      <  2:539755/243‑1 (MQ=255)                                                                           gctgGCGATGGTGTCCAGCCGGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCTGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATaa                                                                                                                                                                       <  1:609692/250‑1 (MQ=255)                                                                            ctgGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATg                                                                                                                                                                     >  1:636584/1‑251 (MQ=255)                                                                               gCGATGGTTTCAAGCCTGCTGGACAGACCGCTGCCACAGGAGCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTAGAATgagg                                                                                                                                                                  <  2:609796/251‑2 (MQ=255)                                                                                        tCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACgga                                                                                                                                                          <  1:525456/250‑1 (MQ=255)                                                                                                     aCAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGGTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATgtcgt                                                                                                                                            <  2:166709/251‑1 (MQ=255)                                                                                                                 cacAGGATCTGGTGGTGTTTGGAGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGGCTTATAATGAGGAATACGGGGGGAGATATGTCGTCTTTTGATTAcc                                                                                                                                >  2:108868/3‑251 (MQ=255)                                                                                                                  acAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATgag                                                                                                                                                                   <  1:159622/214‑1 (MQ=255)                                                                                                                           ggtggtGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAAc                                                                                                                         <  1:564928/248‑1 (MQ=255)                                                                                                                            gtggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGcc                                                                                                                     <  1:123332/251‑1 (MQ=255)                                                                                                                                 gtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa                                                                                                                 >  1:673933/1‑250 (MQ=255)                                                                                                                                 gtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa                                                                                                                 <  1:33711/250‑1 (MQ=255)                                                                                                                                    tGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTCGCGTGTTCGACGACtttt                                                                                                                                                                         >  2:23174/1‑189 (MQ=255)                                                                                                                                    tGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTtat                                                                                                                                                                         <  1:23174/191‑1 (MQ=255)                                                                                                                                              gggCTGGCAGTGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGc                                                                                                   >  1:541912/1‑251 (MQ=255)                                                                                                                                                     cAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAc                                                                                            >  1:415020/1‑251 (MQ=255)                                                                                                                                                        gggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAcagc                                                                                         >  1:622946/1‑251 (MQ=255)                                                                                                                                                                            aGCGGTCAGGAACGAATGTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑Gt                                                                    <  1:499498/251‑1 (MQ=255)                                                                                                                                                                               ggTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACa                                                                                                          <  2:34286/211‑1 (MQ=255)                                                                                                                                                                               ggTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACa                                                                                                          >  1:34286/1‑211 (MQ=255)                                                                                                                                                                                gTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATg                                                                 >  1:227851/1‑250 (MQ=255)                                                                                                                                                                                    ggAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCaa                                                            <  1:465289/251‑1 (MQ=255)                                                                                                                                                                                         gaattccagaattggcgAAACACGGTTTGCGTCGGGCGATTGGGCCGGCGGCGAAGGTACCGAAAAAAGCGCCGGAAAGTATTCAGAGTTTTGGCTTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCTACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCTCGCTACAGCAGGTGGCTGATGCCAGCG‑TTATGACCAAAGGGt                                                       <  2:614445/238‑1 (MQ=255)                                                                                                                                                                                             ttcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTAtt                                                    <  2:636584/246‑1 (MQ=255)                                                                                                                                                                                              tcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTAttt                                                   <  1:516594/247‑1 (MQ=255)                                                                                                                                                                                               cagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTaa                                                 <  1:232563/249‑1 (MQ=255)                                                                                                                                                                                                  aaGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGcc                                              <  1:3872/251‑1 (MQ=255)                                                                                                                                                                                                       gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCAGGACGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGtt                                          >  1:92382/1‑250 (MQ=255)                                                                                                                                                                                                        cgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCTCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGTGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCGATAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTAc                                        <  1:435742/251‑1 (MQ=255)                                                                                                                                                                                                             cacGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGCCGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGt                                                                                     >  2:296111/1‑202 (MQ=255)                                                                                                                                                                                                             cacGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGt                                                                                     <  1:296111/202‑1 (MQ=255)                                                                                                                                                                                                             cacGGTGTTCGCCGGGCGATTGTTCCGGCTGCTAACGTACCGAAAATAGCGCCTGAAGTGATGCAGATTTTTGTCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGTAATACGGAGGGTGATATGTCGTCATTTGATTACCTGAAAACTGCCATCATGCATCATGGCGGCACGCTATAGCGTGTTGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAAt                                   <  1:635613/251‑1 (MQ=255)                                                                                                                                                                                                              acGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATg                                  <  1:313528/251‑1 (MQ=255)                                                                                                                                                                                                                      cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGCCTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCAAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATACCAAAATc                          >  1:364779/1‑251 (MQ=255)                                                                                                                                                                                                                      cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt                           <  1:675795/250‑1 (MQ=255)                                                                                                                                                                                                                       gcagggCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCTGAAGTGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTAGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa                         <  1:357393/248‑1 (MQ=255)                                                                                                                                                                                                                          gggCGATTGTGCACGCGGCTAACGTACAGAAAAAAGCGCCGGAAGGGATGGAGGTTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCTACGACTTATAATGAGGAATCCGGAGGGAGATATGGCGTCATTTGATTACCTGAAAACTGCCATCAAGCCACAGGGCTGCACTCTACAGCAGGTGACTACTGCCA‑CGTGGATGACCCAAAGGTATTTACGCCAGTTACTGACTGCCCAAATccaa                       >  2:89061/1‑247 (MQ=255)                                                                                                                                                                                                                           ggCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAg                     <  1:446311/251‑1 (MQ=255)                                                                                                                                                                                                                               aTTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                 >  1:593209/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc                 >  2:36288/1‑249 (MQ=255)                                                                                                                                                                                                                                    tCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATCTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg             <  2:593209/250‑1 (MQ=255)                                                                                                                                                                                                                                              aaCGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg  <  1:31547/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCG‑GTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTG  >  NC_000913/4626971‑4627458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑