| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 6,239 | G→T | A74E (GCG→GAG) | yaaA ← | DNA binding and peroxide stress response protein YaaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 6,239 | 0 | G | T | 89.8% | 110.6 / ‑2.5 | 52 | A74E (GCG→GAG) | yaaA | DNA binding and peroxide stress response protein YaaA |
| Reads supporting (aligned to +/- strand): ref base G (1/4); new base T (29/15); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTT > NC_000913/6003‑6481 | tgGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGGGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCCTTCTCCGGCTTGGAATCt > 2:609999/1‑251 (MQ=255) gTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTgg > 1:580282/1‑251 (MQ=255) gctgatATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTTGCt > 2:45848/3‑251 (MQ=255) atatCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCa < 1:78756/251‑1 (MQ=255) tctcCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTc > 1:443623/1‑251 (MQ=255) tctcCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTc > 1:83315/1‑251 (MQ=255) tctcCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGACGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCAGGCT‑GCCCGTc > 2:385562/1‑251 (MQ=255) cccccAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCAt < 1:497737/250‑1 (MQ=255) ccccAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCa < 1:30001/249‑1 (MQ=255) aTTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAagcg > 1:463931/1‑250 (MQ=255) tGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAagcgagc > 1:145433/1‑251 (MQ=255) aTACAGATCTTTCCCTCGGGCATTCGTAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCTGGAAAGCATT‑CGCAAATGCGGTTGGGCAAAATCGAAATCGTCTTCGCGGAAGGTTTCGGTCTGC‑AAGCCGGTGGAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCGGGCT‑GCCAGTCATGAAAGCGAgcgg < 2:618537/251‑1 (MQ=255) aCAGATCTTTCCCTCGGGCATTCTCAAGACGGCTCCCCATTTCCAGACGATATGGCTGCCTTAAATCGAGCGGGCGCAGTCCGCCATCCACGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGCAATCGTCTTCGATGAAGGTTTCGGCCTGC‑ACGCCGGTGTAGCCATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCCGTAAGGAAAGCGAgcggcg > 2:305372/1‑251 (MQ=255) ttCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑g < 1:49905/246‑1 (MQ=255) ccccTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGGCATGAAAGCGAGCGGCGTT‑GATAccc < 2:114642/250‑1 (MQ=255) aTCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCAt > 1:294486/1‑251 (MQ=255) tCCCCATTTCCAGACGATAAGGATGCCTTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGAAAATGCTGTTGGGCACAATCGAAATCGTCTTCGATGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCCTGCAGTCTGGCT‑GCCAGTCCTGCAAGGGGGCGGCGTTTGA‑ACCCGCCAGtttttggcttatgagcat > 2:436236/1‑235 (MQ=255) cattgcaaGACGATAAGGCGGCATAAAATCGAGCGGGCGCAGTACCCCATACAAGCCGGAAAGTATT‑CGCAAATGCGGTTGGGCAAAAGCGAAATCGTCTGCGCGTAAGTTTTCGGCCTGC‑AAGCCGGTGTATACATCACCTTTAAACGCCATAATCGCCTGGCGGGCATTCTCCGTCGTGAAATCTGGCG‑GCCAGTCAGGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCg < 1:436236/244‑1 (MQ=255) aTTTCCAGACGATAAGAATGCATTAAATCGAGCGGGCGCAGTACTCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGAAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTACGTAGTGTAATCTGGCT‑GCCAGTCATTAAAGCGCGCGGCATT‑GATACCCGCCAGTTTGACGCTGATGCGCATccgatt > 2:44535/1‑246 (MQ=255) gcaaTAAGGCAGAATTAAAGCGAGCGGGCGCAGTACCCCAACCAGGCCGGAA‑GCCTTGCGAAAATGCTGTTGGGAAAAATCGAAATCTTCTTCGCTGAGGGTTCCGGCCGGA‑AAGCCGGTGTAGACATCACGTTTAACCGCCAGAATCGCCTGGCGGGCATTCCCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGGGAGGGGCGTT‑AATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCt < 2:145433/248‑1 (MQ=255) cATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCAACTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGAAGCGCATCAGCGTG‑CTCATCTGCGGCGGCGTc > 1:99905/1‑250 (MQ=255) aaTCGAACGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACACCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGGCATGAAAGAGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAACCTGCGGAGGCGTCAGTTTc > 1:291768/1‑251 (MQ=255) gagtgggcgcAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCTGCGttt < 2:636121/183‑2 (MQ=255) gAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑g > 1:636121/1‑187 (MQ=255) aaGCGGACGCAGTACGCCATACAAAACGGAAAGCATT‑CGCAAATGCTGTTGGGCACAATCGAAATCGTCTTCGCTGAAGGTTTAGGCCTGC‑AAGCCGGTGTAGACATCACCTTTACACGCCAGAATCGCCTGGCGGGCCTTCTCCGGCGTGAAATCTGGCG‑GCACGTCCAGAAAGCTCGCGGATTT‑GATCCCCGCAAGTTTGTGTCTTGTGCG‑ATAAGCATGGCTAATCTGCGGAGGGGTCAGTTTccacg > 2:501103/2‑248 (MQ=255) ggggcgcAGTCCGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCgcgctt < 1:45848/250‑3 (MQ=255) cctcaacccccctcttccattctgCAAATGTTGTTGGGCAAAACCAAAACCGTCTTCGCTGAAGGTTTCGGC‑TGCCAAGCCGTTGTAGACATCACCTTAAACCGCCAGAATCGCCTGGCGGGAATTCCCCGGCGTAAAATTTGGCT‑GCCAGTCATAAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAAct < 2:386061/228‑1 (MQ=255) cATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGAAAAATCGAAATCGTCTCCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAActgc < 1:659388/251‑1 (MQ=255) aTACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAActgct > 1:153083/1‑251 (MQ=255) cGGAAAGCATT‑CGCAAATGATGTTGGGCAAAATCGAAATCGTCTTAGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCCGTCATGAAGGAGAACGGCGTT‑GATACCCGCCAATTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAAGTTCCGCGCTTCATGGATAAACGTCTGGGAATTg > 2:676507/1‑251 (MQ=255) aaaGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTGTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCt < 1:166533/251‑1 (MQ=255) aaGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCt > 1:615531/1‑250 (MQ=255) aGCATT‑CGCAACTGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCAGTGTAGACATCACCTTTAAACGCAAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGGTTACGCGCTTCATGGATCAACTGCTGGGAATTGTCTa > 1:372901/1‑250 (MQ=255) aTT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAATCGCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAAc > 1:631213/1‑249 (MQ=255) agCAAATGCTGTTAGGCAAAATGGAAATAGTCTTCGCTGAAGGTCTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGACAAAATCGCATGGCGGGCATTCTACGGCGTGACATATGGCT‑GCAAGTCATGAAAGAGAGGGACGTT‑GATACCCACAATATTGACGCTGATGCGGAACAACGTG‑CATATATGAGGAGGAGTCGGGTTACTCGCTTCATGGCACAACGGCTGGGAATGTTCTAACAGCTc > 2:652653/2‑251 (MQ=255) gCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAAct > 1:386061/1‑228 (MQ=255) gCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTcc > 1:396470/1‑251 (MQ=255) aaTGCTGTTGGGAAAAATCGAAATCGTCTCCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACACCCCCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGttt < 1:374004/160‑1 (MQ=255) tGCTGTTGGGCAAAATCGAACTCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGACAGCGAGCGGCGTT‑GATACCCGCCAGTTTTTCGCTGATGCGCATCCGAGTG‑CTACACTGCGGAGGCGTAAGTTTCAGCGCTTCCTGGATCAACTGATGGGAATTGTTTACCAGCTCCGGccg > 2:634000/1‑249 (MQ=255) cAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAgcgc > 1:466424/1‑251 (MQ=255) aaaaTCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGcgcg > 1:494217/1‑251 (MQ=255) ccgaaatcgTCTCAGCTGAAGGTTCCGGCCTGA‑AAGCCGGTGTAGACATCACTTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCgtggt < 1:664721/250‑1 (MQ=255) aaatcgTCGTCACTGAAGGTTTCGGACTGC‑AAGCCGGTGTAGACATCACCTTTAACCGCCAGAATCGCCTGGCGGGGATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGGGCGGCGTT‑GATACCCGCCAGTTTGGCGCTGATGCGCATCAGCGTG‑CTAATATGCGGAGGCGTGAGTTTCCGAGCTTCAAGGATCAAATGAGGGGCATTATCTGACAGCTCGGGCAGCGTAAAGCGCGtggtggg > 2:333223/1‑250 (MQ=255) tCGCGGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGCCCTCACCTTTAAACGCCAGAATCGCCTGGCGGCCATTCTCCGGCGTGAAATCGGGCT‑GCAAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACgg < 1:373925/248‑1 (MQ=255) gCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCttt > 1:434204/1‑251 (MQ=255) atggTTGCGGCCTGC‑AAGCCGTCGGAGAAACCCCCTTAAAACGCCAGAATTGCGTGCCGGGCACTCGCCGGCGATAAGTATGGCT‑GCATGTCATGAAAGCGAGTGTGGTT‑GATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGGGGTGGTCAACGTGCTTTGGTa < 2:631213/249‑1 (MQ=255) tttCGGCCTGC‑AGGCCGGTGGAGACATCACCTTTAAACGCAAGAACCGCCTGGCGGGCATGCTCCGGCGTGAAATCGGGCT‑GCCGGTCATGAAAGCGAGGGGGGTT‑GATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATGGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATc < 1:305372/250‑1 (MQ=255) cggctggaaaGCCGGTGTAGACATCACCTTAAAACGCCATAAGCCTCTGGCGGGCATTCTCCGGTGTGAAATCTGGCT‑GCCCGTCATGAAAGCGGGCGGGGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGc < 1:126040/243‑1 (MQ=255) ccGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAgg > 1:109737/1‑251 (MQ=255) ccgggCGGGCATTCTCTGGCGGTAAATCTGGCT‑GCCAGTCAGGAAAGCGAGCGGTGTT‑GATACCCGCCAGTTTGGCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAgg < 1:634000/248‑1 (MQ=255) ggCGGGCATTCGCCGGCGTGAAGTC‑GGCTCGCCAGTCATGAAAGCGGGGGGCGTT‑TACACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCCGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGa < 1:263979/249‑1 (MQ=255) gggCATTCCCCGGCGGAAAATCTGGCT‑GCCAGTTATAAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAAttt < 1:518608/251‑1 (MQ=255) | TGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATT‑CGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGC‑AAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCT‑GCCAGTCATGAAAGCGAGCGGCGTT‑GATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTG‑CTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTT > NC_000913/6003‑6481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |