Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,622	C→T	V56V (GTG→GTA)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,622	0	C	T	100.0%	222.8 / NA	70	V56V (GTG→GTA)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/36);  total (34/36)

TTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGT‑TCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTG  >  NC_000913/4624383‑4624868                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        tttttCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTGCCGGCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCAGCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTGCCTGAcgcg                                                                                                                                                                                                                                               <  2:31257/250‑1 (MQ=255)      cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGACCGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAggg                                                                                                                                                                                                                                         >  1:384792/5‑251 (MQ=255)      cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAgg                                                                                                                                                                                                                                          <  1:567033/246‑1 (MQ=255)       ccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCCgggg                                                                                                                                                                                                                                        >  2:308691/4‑250 (MQ=255)         ggCAATTGTGTCGACATTCTGCTGGTTAAAATAGCCGCCAGGTTTTTTACCGCGGAGCGCCAGCCGGTCGCGCACGTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑GCGTCATACACGCCCGCGTCGAGGATACGGCTTTCAGCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGc                                                                                                                                                                                                                                      <  2:98986/250‑1 (MQ=255)            aaTTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCggg                                                                                                                                                                                                                                   >  1:425255/1‑251 (MQ=255)            aaTTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCggg                                                                                                                                                                                                                                   <  1:330109/251‑1 (MQ=255)                    cGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACCTTCATGGTTACCTGACGCGCCCGGGTGCGGGCCAGttct                                                                                                                                                                                                                           >  2:419664/1‑251 (MQ=255)                                    aaaaTAGCCGCCCGCTTTTTAACCGCGGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTAc                                                                                                                                                                                                           <  2:150537/251‑1 (MQ=255)                                          cccgcccgcTTTTTTACCGGCGAGCGCCAGCCGGTCGCGCACTGCGACGTCTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCAGACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTc                                                                                                                                                                                                     <  2:410702/250‑1 (MQ=255)                                              cccgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGCTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTCCGTTGGCGCtg                                                                                                                                                                                                 >  2:664578/4‑251 (MQ=255)                                              cccgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGAGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCCGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGATGTCGCtg                                                                                                                                                                                                 >  2:621571/4‑251 (MQ=255)                                                cgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCTCTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGGCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGGTACCTGACGCGCCAGGGTGCGGGTCAGTTCTTCCAGCTGTGGATTACGTTGTCGCtggt                                                                                                                                                                                               <  2:348039/250‑1 (MQ=255)                                                 gtttgtttACCGGCGAGTGCCCGCTGATCGTGCACTTCGACGCTTTCGCCAGAACCGGCGATAAGATCGCCT‑GTGTCGTCAGACCCGCCCGCGTCGAGGATACGGCTTTCATCCTGTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGTCGAGTCGGTACTCATCAGCGGTGCAACGTGCATCTTTACCTGTCGCGCCAGGGTTCGTGCCAGTTCTTCCAGCATTGGATTActttgtctctggga                                                                                                                                                                                              <  2:114783/246‑14 (MQ=255)                                                   ttttttCCCGTCGAGCGCCAGCCGGTCGCGCCCTTCGACGCTTTCGCCATAGCGGGCGTTTAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGTCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGTtggtgg                                                                                                                                                                                             <  2:432368/250‑1 (MQ=255)                                                     ttttACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGtt                                                                                                                                                                                           <  1:463936/250‑1 (MQ=255)                                                              cGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGACCGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGGGGTTTTGAATaatc                                                                                                                                                                                 >  2:305276/1‑249 (MQ=255)                                                                  cgcCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATg                                                                                                                                                                             >  1:522389/1‑251 (MQ=255)                                                                     cAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGCATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCCACGTTCAGCGTTACCTCACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                                                                                                          >  2:225354/1‑251 (MQ=255)                                                                        ccGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTc                                                                                                                                                                       >  1:491351/1‑251 (MQ=255)                                                                        ccGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCAACAGCGGAGCAACGTTCAGCGTTACCTGTCGCGCCAGGGAGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTTACTAAACCCTGACGCTc                                                                                                                                                                       >  1:454807/1‑251 (MQ=255)                                                                           gTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCt                                                                                                                                                                    <  2:491351/251‑1 (MQ=255)                                                                                    ttCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGGTTACGTTGTCGCTGGTGGTTTTGACTCAACCATGACGCTCCCTGCATCAgcg                                                                                                                                                           >  2:317253/1‑251 (MQ=255)                                                                                       gACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAgc                                                                                                                                                            >  1:194562/1‑247 (MQ=255)                                                                                       gACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGcc                                                                                                                                                         >  1:62972/1‑250 (MQ=255)                                                                                       gACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTGTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGGACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAgc                                                                                                                                                            <  2:194562/247‑1 (MQ=255)                                                                                                              aaaGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAgg                                                                                                                                 <  2:58007/250‑1 (MQ=255)                                                                                                               aaGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAgg                                                                                                                                 >  1:155489/1‑250 (MQ=255)                                                                                                                   tCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAAt                                                                                                                            >  1:483642/1‑251 (MQ=255)                                                                                                                         tGT‑TAGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGCTCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGGGCGGGCCAGTTCTTCCAGCTGTGGATTAAGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGAATCAGCGCCACTAACAAGGCAAGACAGAACAGGCCAATCACTGc                                                                                                                      >  2:171024/1‑251 (MQ=255)                                                                                                                                tcatgcacgcccggtgctaGGATACGGCTTTCATCCGTTAACTGTTCGAGGATCGCCTGAAGTCGTTTTTCATCCGGCGAGTCGTTACGCATCAGCGGTGCAACGTTCAGCGGTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATGTCGTTGGCGCTGTTGGGTTTGACTAAACCATGACGCGCCCTGCATCAGCGCCACGAACAAGGCAAGACAGAACAGTGCAATCACTGCCCGAt                                                                                                                 <  2:384792/232‑1 (MQ=255)                                                                                                                                  aTACACACCCGCGACGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCAGAATACGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCCGGGAGCGGGCCAGTTCTTCCAGCTGGGGATTACGTTGTCGCTTGTGGTTTTGACTAAACCATGACGCTCCCTGCATTAGCGCCACTAACAAGGCACGACAGAACAGGACAATCACTGCCAGAT‑Gcc                                                                                                             >  2:50341/1‑250 (MQ=255)                                                                                                                                      acgcccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGACGGTACGCATCAGCGGTGCAACGTTAAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGAGCCACTAAAACGGCAAGACAGAACAGGACAATCACTGCCCGAT‑ACAGCCg                                                                                                         >  1:480953/4‑251 (MQ=255)                                                                                                                                          ccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAAt                                                                                                     >  1:164333/1‑251 (MQ=255)                                                                                                                                          ccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGGTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGGCCGAT‑GCAGCCGGAAt                                                                                                     >  1:597717/1‑251 (MQ=255)                                                                                                                                          ccGCGGCGATGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAAt                                                                                                     <  1:329231/251‑1 (MQ=255)                                                                                                                                           cgcgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAAtt                                                                                                    <  1:317253/251‑1 (MQ=255)                                                                                                                                             cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTc                                                                                                  <  1:50341/251‑1 (MQ=255)                                                                                                                                              gTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCa                                                                                                 <  1:294770/251‑1 (MQ=255)                                                                                                                                                cGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGt                                                                                               >  1:536740/1‑251 (MQ=255)                                                                                                                                                 gcggctaaggCTTTCATCCGTTAACTGATCAAGGATCGCCTGACTGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTAACTGACGCACCCGGGTGCGGGCCCGTTCTTCCAGCTGTGGAGTACGTTGACGCTGGTGGTTTTGACTAAACCATGACGCTACCTGCATCAGCGACACTACCAAGGCAAGACAGAACAAGACAACCACTGCCC‑ATAGCAGCCGGAATTTCCGtt                                                                                              >  2:256103/9‑251 (MQ=255)                                                                                                                                                    gATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGccagcca                                                                                                                                        >  2:182997/1‑201 (MQ=255)                                                                                                                                                    gATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACa                                                                                                                                        <  1:182997/207‑1 (MQ=255)                                                                                                                                                    gATACGGCTTTCATCCGTTAACTGAGCGAGGAGCGCCTGAATGCGTTTTTAAGCCGGCGAGTCGGTACGCATCATCGGTGCAACGTGCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTtg                                                                                           <  2:425255/251‑1 (MQ=255)                                                                                                                                                            tttCATCCGTTAACTGAACGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCCGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGGGGTTTTGACTAAACAATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACCGGACAATCACTGCCCGAT‑GCAACCGGATTTACAGTGTTGTGCGAgtc                                                                                   >  2:116165/1‑249 (MQ=255)                                                                                                                                                              tCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                                  <  1:308691/250‑1 (MQ=255)                                                                                                                                                              tCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCa                                                                                  <  1:278277/250‑1 (MQ=255)                                                                                                                                                               cATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGATGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATc                                                                                <  1:361508/251‑1 (MQ=255)                                                                                                                                                               cATCCGTTAACTGATCGAGGATCGCATGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGTTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCAt                                                                                 <  1:632227/250‑1 (MQ=255)                                                                                                                                                                          tGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAAGCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCttt                                                                     >  1:451455/1‑251 (MQ=255)                                                                                                                                                                           gATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTg                                                                    <  1:225354/251‑1 (MQ=255)                                                                                                                                                                           gATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCAATAACAAGGCACGACAGAACAGGACAATAACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGTGACATCTTCCACCCTTTg                                                                    >  2:15169/1‑251 (MQ=255)                                                                                                                                                                               gAGGATCGCCTGAATGCGTTTTTCATCCGGCGCGTCGGTACGAATCAGCGGTGCAACGTTAAGCGTTACCTTACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACACTCCCTGCATCAGCACCACTAACAAGGCGCTACAGAACAGGACAATCAATGCCCGGT‑GAAGCCGGACTTTCCGTTTTGTTCGAGACATCTTTCTCCCTTTGaga                                                                 >  2:586707/1‑248 (MQ=255)                                                                                                                                                                                 ggATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCAGCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCGTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCGGCATCAGCTCCTCTAACAAGGCAAGACTGAACAGGACAATCACTGCCCTAT‑GCAGCCGGAATTTCAGTTGTGTGCGAGCCATCTTCCACCCTTTGAAAAtt                                                              <  1:324454/251‑1 (MQ=255)                                                                                                                                                                                   aTCGCCTGAATGCGTTTTTCATCCGTCGAGTCGTTACGCATCATCGGTGCATCTTTCAGCTTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTg                                                            <  1:50122/251‑1 (MQ=255)                                                                                                                                                                                              gCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATg                                                  <  1:340132/250‑1 (MQ=255)                                                                                                                                                                                              gCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGt                                                 <  1:61466/251‑1 (MQ=255)                                                                                                                                                                                                gTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTg                                               >  1:673057/1‑251 (MQ=255)                                                                                                                                                                                                gTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGGTCGAGCCATCTTCCACCCTTTGGAAATTTGAGACTTAATGTTg                                               >  2:123965/1‑251 (MQ=255)                                                                                                                                                                                                 tttttCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAAc                                                                                                                                                   <  1:376492/151‑1 (MQ=255)                                                                                                                                                                                                 tttttCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGAGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATAACGCTCCCTGCATCCGCGCCACTAAc                                                                                                                                                   >  2:376492/1‑151 (MQ=255)                                                                                                                                                                                                     tCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCAGCTTCCACCCTGTGAAAATTTGAGACTTAATGTTGCCAg                                           <  1:267660/250‑1 (MQ=255)                                                                                                                                                                                                       aTCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGGTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGaa                                         >  2:375863/1‑250 (MQ=255)                                                                                                                                                                                                         ccGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCa                                      <  1:232141/251‑1 (MQ=255)                                                                                                                                                                                                          cggcgAGTCGGTACGCAGCAGCGGTGCAACGTTCAGCGTTACCTGACGCGACAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGGCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCAGCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCaa                                     <  2:114486/247‑1 (MQ=255)                                                                                                                                                                                                                            cAGCGGTGCACCGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCCTTGAAAATTTGAGACTTAAGGTTGCCAGAAGCAATGGATACAAGGTAGCCTc                   <  1:586707/251‑1 (MQ=255)                                                                                                                                                                                                                             aGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTc                   >  1:180288/1‑250 (MQ=255)                                                                                                                                                                                                                              gCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCAt                 <  1:375863/251‑1 (MQ=255)                                                                                                                                                                                                                               cGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATg                <  1:256103/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCGTTACCTGACGCGCCAGCGTGCGGGACAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGATCCCTGCATCAGCGCCACTAACAAGGCACGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGAAGCCTAATGCGGTATTTTcccgg  >  2:665292/1‑249 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        TTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGT‑TCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTG  >  NC_000913/4624383‑4624868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑