| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,624,586 | T→C | S68S (TCA→TCG) | ytjB ← | putative inner membrane protein Smp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,624,586 | 0 | T | C | 98.6% | 217.9 / ‑4.7 | 70 | S68S (TCA→TCG) | ytjB | putative inner membrane protein Smp |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (36/33); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
GGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGT‑TCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCA > NC_000913/4624345‑4624832 | gggTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGT‑TCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGtc > 1:226254/1‑250 (MQ=255) ggTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTACGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTAc < 1:328791/251‑1 (MQ=255) tgtCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGc > 1:148078/1‑251 (MQ=255) gtCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCgg > 1:58007/1‑251 (MQ=255) aGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGc > 1:1217/1‑251 (MQ=255) ggTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGCGGCTCGCCTGACTGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGGGCAAGGTTCAGCGtttcctg > 2:329231/1‑245 (MQ=255) tttttCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTGCCGGCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCAGCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTGCCTGAcgcg < 2:31257/250‑1 (MQ=255) cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGACCGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAggg > 1:384792/5‑251 (MQ=255) cccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAgg < 1:567033/246‑1 (MQ=255) ccggCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCCgggg > 2:308691/4‑250 (MQ=255) ggCAATTGTGTCGACATTCTGCTGGTTAAAATAGCCGCCAGGTTTTTTACCGCGGAGCGCCAGCCGGTCGCGCACGTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑GCGTCATACACGCCCGCGTCGAGGATACGGCTTTCAGCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGc < 2:98986/250‑1 (MQ=255) aaTTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCggg > 1:425255/1‑251 (MQ=255) aaTTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCggg < 1:330109/251‑1 (MQ=255) cGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACCTTCATGGTTACCTGACGCGCCCGGGTGCGGGCCAGttct > 2:419664/1‑251 (MQ=255) aaaaTAGCCGCCCGCTTTTTAACCGCGGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTAc < 2:150537/251‑1 (MQ=255) cccgcccgcTTTTTTACCGGCGAGCGCCAGCCGGTCGCGCACTGCGACGTCTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCAGACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTc < 2:410702/250‑1 (MQ=255) cccgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGCTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTCCGTTGGCGCtg > 2:664578/4‑251 (MQ=255) cccgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGAGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCCGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGATGTCGCtg > 2:621571/4‑251 (MQ=255) cgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCTCTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGGCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGGTACCTGACGCGCCAGGGTGCGGGTCAGTTCTTCCAGCTGTGGATTACGTTGTCGCtggt < 2:348039/250‑1 (MQ=255) gtttgtttACCGGCGAGTGCCCGCTGATCGTGCACTTCGACGCTTTCGCCAGAACCGGCGATAAGATCGCCT‑GTGTCGTCAGACCCGCCCGCGTCGAGGATACGGCTTTCATCCTGTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGTCGAGTCGGTACTCATCAGCGGTGCAACGTGCATCTTTACCTGTCGCGCCAGGGTTCGTGCCAGTTCTTCCAGCATTGGATTActttgtctctggga < 2:114783/246‑14 (MQ=255) ttttttCCCGTCGAGCGCCAGCCGGTCGCGCCCTTCGACGCTTTCGCCATAGCGGGCGTTTAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGTCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGTtggtgg < 2:432368/250‑1 (MQ=255) ttttACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGtt < 1:463936/250‑1 (MQ=255) cGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGACCGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGGGGTTTTGAATaatc > 2:305276/1‑249 (MQ=255) cgcCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATg > 1:522389/1‑251 (MQ=255) cAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGCATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCCACGTTCAGCGTTACCTCACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACg > 2:225354/1‑251 (MQ=255) ccGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTc > 1:491351/1‑251 (MQ=255) ccGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCAACAGCGGAGCAACGTTCAGCGTTACCTGTCGCGCCAGGGAGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTTACTAAACCCTGACGCTc > 1:454807/1‑251 (MQ=255) gTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCt < 2:491351/251‑1 (MQ=255) ttCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGGTTACGTTGTCGCTGGTGGTTTTGACTCAACCATGACGCTCCCTGCATCAgcg > 2:317253/1‑251 (MQ=255) gACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAgc > 1:194562/1‑247 (MQ=255) gACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGcc > 1:62972/1‑250 (MQ=255) gACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTGTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGGACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAgc < 2:194562/247‑1 (MQ=255) aaaGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAgg < 2:58007/250‑1 (MQ=255) aaGATCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAgg > 1:155489/1‑250 (MQ=255) tCGCCTTGT‑TCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAAt > 1:483642/1‑251 (MQ=255) tGT‑TAGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGCTCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGGGCGGGCCAGTTCTTCCAGCTGTGGATTAAGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGAATCAGCGCCACTAACAAGGCAAGACAGAACAGGCCAATCACTGc > 2:171024/1‑251 (MQ=255) tcatgcacgcccggtgctaGGATACGGCTTTCATCCGTTAACTGTTCGAGGATCGCCTGAAGTCGTTTTTCATCCGGCGAGTCGTTACGCATCAGCGGTGCAACGTTCAGCGGTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATGTCGTTGGCGCTGTTGGGTTTGACTAAACCATGACGCGCCCTGCATCAGCGCCACGAACAAGGCAAGACAGAACAGTGCAATCACTGCCCGAt < 2:384792/232‑1 (MQ=255) aTACACACCCGCGACGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCAGAATACGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCCGGGAGCGGGCCAGTTCTTCCAGCTGGGGATTACGTTGTCGCTTGTGGTTTTGACTAAACCATGACGCTCCCTGCATTAGCGCCACTAACAAGGCACGACAGAACAGGACAATCACTGCCAGAT‑Gcc > 2:50341/1‑250 (MQ=255) acgcccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGACGGTACGCATCAGCGGTGCAACGTTAAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGAGCCACTAAAACGGCAAGACAGAACAGGACAATCACTGCCCGAT‑ACAGCCg > 1:480953/4‑251 (MQ=255) ccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAAt > 1:164333/1‑251 (MQ=255) ccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGGTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGGCCGAT‑GCAGCCGGAAt > 1:597717/1‑251 (MQ=255) ccGCGGCGATGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAAt < 1:329231/251‑1 (MQ=255) cgcgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAAtt < 1:317253/251‑1 (MQ=255) cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTc < 1:50341/251‑1 (MQ=255) gTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCa < 1:294770/251‑1 (MQ=255) cGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGt > 1:536740/1‑251 (MQ=255) gcggctaaggCTTTCATCCGTTAACTGATCAAGGATCGCCTGACTGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTAACTGACGCACCCGGGTGCGGGCCCGTTCTTCCAGCTGTGGAGTACGTTGACGCTGGTGGTTTTGACTAAACCATGACGCTACCTGCATCAGCGACACTACCAAGGCAAGACAGAACAAGACAACCACTGCCC‑ATAGCAGCCGGAATTTCCGtt > 2:256103/9‑251 (MQ=255) gATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGccagcca > 2:182997/1‑201 (MQ=255) gATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACa < 1:182997/207‑1 (MQ=255) gATACGGCTTTCATCCGTTAACTGAGCGAGGAGCGCCTGAATGCGTTTTTAAGCCGGCGAGTCGGTACGCATCATCGGTGCAACGTGCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTtg < 2:425255/251‑1 (MQ=255) tttCATCCGTTAACTGAACGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCCGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGGGGTTTTGACTAAACAATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACCGGACAATCACTGCCCGAT‑GCAACCGGATTTACAGTGTTGTGCGAgtc > 2:116165/1‑249 (MQ=255) tCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCa < 1:308691/250‑1 (MQ=255) tCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCa < 1:278277/250‑1 (MQ=255) cATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGATGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATc < 1:361508/251‑1 (MQ=255) cATCCGTTAACTGATCGAGGATCGCATGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGTTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCAt < 1:632227/250‑1 (MQ=255) tGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAAGCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCttt > 1:451455/1‑251 (MQ=255) gATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTg < 1:225354/251‑1 (MQ=255) gATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCAATAACAAGGCACGACAGAACAGGACAATAACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGTGACATCTTCCACCCTTTg > 2:15169/1‑251 (MQ=255) gAGGATCGCCTGAATGCGTTTTTCATCCGGCGCGTCGGTACGAATCAGCGGTGCAACGTTAAGCGTTACCTTACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACACTCCCTGCATCAGCACCACTAACAAGGCGCTACAGAACAGGACAATCAATGCCCGGT‑GAAGCCGGACTTTCCGTTTTGTTCGAGACATCTTTCTCCCTTTGaga > 2:586707/1‑248 (MQ=255) ggATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCAGCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCGTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCGGCATCAGCTCCTCTAACAAGGCAAGACTGAACAGGACAATCACTGCCCTAT‑GCAGCCGGAATTTCAGTTGTGTGCGAGCCATCTTCCACCCTTTGAAAAtt < 1:324454/251‑1 (MQ=255) aTCGCCTGAATGCGTTTTTCATCCGTCGAGTCGTTACGCATCATCGGTGCATCTTTCAGCTTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTg < 1:50122/251‑1 (MQ=255) gCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATg < 1:340132/250‑1 (MQ=255) gCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGt < 1:61466/251‑1 (MQ=255) gTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTg > 1:673057/1‑251 (MQ=255) gTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGGTCGAGCCATCTTCCACCCTTTGGAAATTTGAGACTTAATGTTg > 2:123965/1‑251 (MQ=255) tttttCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAAc < 1:376492/151‑1 (MQ=255) tttttCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGAGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATAACGCTCCCTGCATCCGCGCCACTAAc > 2:376492/1‑151 (MQ=255) tCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCAGCTTCCACCCTGTGAAAATTTGAGACTTAATGTTGCCAg < 1:267660/250‑1 (MQ=255) aTCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGGTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGaa > 2:375863/1‑250 (MQ=255) ccGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCa < 1:232141/251‑1 (MQ=255) | GGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGT‑TCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGAT‑GCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCA > NC_000913/4624345‑4624832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |