Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,037	T→C	Q27Q (CAA→CAG)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,037	0	T	C	96.8%	267.3 / ‑2.1	93	Q27Q (CAA→CAG)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (35/55);  total (37/56)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTGC‑GTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCA  >  NC_000913/4623791‑4624281                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        cAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGc                                                                                                                                                                                                                                                     <  2:186391/251‑1 (MQ=255)        ttGAGCACAAACGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGAATTGCCGGGATCGTGGAAAACCGCGCCGCAACCGCAAC‑TGCGCCGTGCCAGGCGGGCGTTATCTACTTCCATCCGCCGGGGCTTAACCTCTTTCACCGAGTTCGGCGCGCGACCATTCAC‑CCCGATGTAGGCATTCCGCCAGAGAACAAGAACGAGCTGCGTG‑CGGGCCACTTGACGaaaaa                                                                                                                                                                                                                                               >  2:477607/1‑250 (MQ=255)              acaATAGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACAAATCACTCCCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGCCGGGC‑CTCTGGCGAAACATACActc                                                                                                                                                                                                                                        >  2:574751/1‑251 (MQ=255)               caATCGACGTGGAGATGGTTGTACCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGAGCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACActcc                                                                                                                                                                                                                                       <  1:131759/251‑2 (MQ=255)                aaTCGGCGTGGAGATGGTTTTATCGTACCCCGGTGTGCCAGCCATAAAGGTAAAGCAGGTAGTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACGCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATGCActcct                                                                                                                                                                                                                                      <  2:498080/251‑3 (MQ=255)                aaTCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACActcct                                                                                                                                                                                                                                      <  1:329840/251‑3 (MQ=255)                   cGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAAATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCTTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑GTTCGGCTTTGCGCCAGGGAAACAGAACGCGCTGCGTGGCGGGC‑ACCGGGCGAAAAATACACtcctcc                                                                                                                                                                                                                                    >  2:238040/1‑250 (MQ=255)                   cGACGTGGAGATGGTTTTATCGTACGCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTAGCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCa                                                                                                                                                                                                                                   <  1:109943/251‑1 (MQ=255)                                  tttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAc                                                                                                                                                                                                                     <  1:224981/250‑1 (MQ=255)                                  tttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAc                                                                                                                                                                                                                     <  1:611465/250‑1 (MQ=255)                                   ttctcgttccccGGTTTGCCAGCCATAAAGGTAAAGCAGGTATGGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGGGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACTCTCCTCCACCGCCAGGTTAAAcc                                                                                                                                                                                                                    <  2:485690/240‑1 (MQ=255)                                   ttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAccc                                                                                                                                                                                                                   >  1:464672/1‑250 (MQ=255)                                    tATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCAc                                                                                                                                                                                                                  >  1:243935/1‑251 (MQ=255)                                            tCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGCCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGt                                                                                                                                                                                                           >  1:248500/1‑250 (MQ=255)                                               ggatggccagacaAAAAGGAAAAGCAGGTATAGCAAAGAGCGTGGAACCACGCGCCGACACCGCTAC‑TGCGCCTTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATGACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCAAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAaga                                                                                                                                                                                                       <  2:295172/246‑1 (MQ=255)                                                   tGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTc                                                                                                                                                                                                    >  1:19881/1‑250 (MQ=255)                                                     ccagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATCCACTCCTCCACCGCCCGGTTAAACCACGGGTCGGAAGAGTccg                                                                                                                                                                                                  >  2:231139/1‑248 (MQ=255)                                                      cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCagag                                                                                                                                                                                                <  2:212519/251‑1 (MQ=255)                                                      cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCTGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGACAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCag                                                                                                                                                                                                  >  1:472910/1‑249 (MQ=255)                                                        gccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGAt                                                                                                                                                                                              <  1:669156/251‑1 (MQ=255)                                                         ccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATg                                                                                                                                                                                             >  1:312729/1‑251 (MQ=255)                                                         ccaTAAAGGGAAAGCAGGTATTGCCGAGATCGTGTAACACCCCGCCGCCACCGCTACGTG‑TCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGTTATTACACTCTTTCCCCGGGTTCTGCGCGCGACCAATCACTCCCG‑TGTCGGCATTGCGCCAGAGAAACAGAACTCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCTCCCCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATg                                                                                                                                                                                             <  2:652859/251‑1 (MQ=255)                                                          caTAAAGGTAAAGCAGGTATGGCCGAGATCGTGGAACACCGCGCCGCCCCCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGa                                                                                                                                                                                            <  1:117934/251‑1 (MQ=255)                                                             aaaGGTAAAGCAGGGATTGCCGTAAGCGTGGAACCCCGCGCCGCCACAGGTTC‑TGCTCCGTGCCAGTCGGCAGTTATCGTCTTCCACCCGCAGGGGATTACTCGCTTTCCACGGGTCCTGCGCGCGACCAATCACGTCCG‑TGCCGGCATTGCGCCAGAGAACCAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGCGATGAGCa                                                                                                                                                                                         <  2:506825/251‑1 (MQ=255)                                                              aaGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGc                                                                                                                                                                                          >  1:222499/1‑249 (MQ=255)                                                               aGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAgg                                                                                                                                                                                       <  1:184275/251‑1 (MQ=255)                                                               aGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAgg                                                                                                                                                                                       <  1:238040/251‑1 (MQ=255)                                                                 gTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCGCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCg                                                                                                                                                                                     <  1:382994/251‑1 (MQ=255)                                                                  tAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGCGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTCAGAGTCAGGGCTGAGCAGGCg                                                                                                                                                                                     >  2:139829/1‑250 (MQ=255)                                                                   aaaGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                                                                                                                                                    <  1:186437/250‑1 (MQ=255)                                                                    aaGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGCAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTa                                                                                                                                                                                   >  1:320700/1‑250 (MQ=255)                                                                     aGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                                                                                 <  1:661649/251‑1 (MQ=255)                                                                      gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                                                                                                                                                <  1:6609/251‑1 (MQ=255)                                                                      gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                                                                                                                                                >  1:251036/1‑251 (MQ=255)                                                                      gCAGGTATTGCCGAGATCGTGGAACACCGCGACGCCACCGCTAC‑TGAGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCACCAACGCCAGGTTAAACCACGGGACGTAAGAGTCAGAGATGAGCTGGCGTAAtg                                                                                                                                                                                >  1:272528/1‑251 (MQ=255)                                                                        aGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                                                                                                                                              >  1:375496/1‑251 (MQ=255)                                                                            aTTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAc                                                                                                                                                                           <  1:364834/250‑1 (MQ=255)                                                                              tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAt                                                                                                                                                                         >  1:46955/1‑250 (MQ=255)                                                                               gCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGATATATTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACAACGGGGCGTAAGAGTCAGCGCAGAGCCGGCGTAATGTGGACATa                                                                                                                                                                        >  2:617043/1‑250 (MQ=255)                                                                               gCCGAGATCGGGGAACACCGCGGCGCCACCGCTAC‑GGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATa                                                                                                                                                                        <  1:472482/250‑1 (MQ=255)                                                                                ccGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATGGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAc                                                                                                                                                                      <  1:489599/251‑1 (MQ=255)                                                                                   ggaTCGTGGAACCCCGCGCCGCCACCGCTAC‑GGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATATCGAt                                                                                                                                                                   <  2:296500/250‑1 (MQ=255)                                                                                   ataTCGGGGAACCCCGCGCCTCCACCGCTAC‑TGCGCCGCGCCAGGCGTACGGTATCTTCGTCCTGCCGCCGGGTATTACACTCGTTCCACGGGTTCGGCGCGCGACCAAGCTCTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCTTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACTGGTTGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGaa                                                                                                                                                                   <  2:83547/249‑2 (MQ=255)                                                                                   agaTCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                                                                                                                                                   <  1:476665/251‑1 (MQ=255)                                                                                         tGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                                                                                                                                             >  1:5642/1‑251 (MQ=255)                                                                                          gtaacaccctgccgccACCGCTAC‑TTCGCCGTGCCCGGCGGACTGTATCTGCTTCCACCCGCCGGTTTTTACTCTCTTGCCACGGGTTCTGCGCGCGACCTAGCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGTCGGTA‑ATCGGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                            <  2:351187/241‑1 (MQ=255)                                                                                                           ccGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCa                                                                                                                                           <  1:577112/251‑1 (MQ=255)                                                                                                             gCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                                                                                                                                         <  1:214018/251‑1 (MQ=255)                                                                                                              gctccggcgccggtccAGGCTGGCGTTATCCTCTTCCCCCCGCCGGGTATTACTCTCTTTCCACCGGTTCTGCGCGCGACCAATCTCCACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCCCCGCCAGGTTAAACCACGGGTAGTAAGAGTCAGAGATGAGCAGGCGTTATCTTGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                                                                                                                                         <  1:272564/237‑1 (MQ=255)                                                                                                                 acggcgccgtgccAGGCGGACGTTATCCTCTTCCATCCGCCGGGTATTACTCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCAAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                                                                                                                                        <  1:16325/246‑1 (MQ=255)                                                                                                                  ccgcgccgtgccAGGCTGACGTGATCTTCTTCCCTCCGCCGGGTATTACACGCTTTCCACGGGTTCGGCGCGCGACCAATCACTCCCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCGTGTAGTAATGGGTCACTCttt                                                                                                                                     <  2:338183/249‑1 (MQ=255)                                                                                                                    cggccgtgcatgccGGACGTTATCTGCCTCCATCCGCCGGTTATTACACGCTTTCCACTGGTTCGGCGCGCGACCAATCACTACCG‑TGCCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑AGCTGGCGAAAAATTCACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTTATGTGGACAGAACGATTTCCTTTCTTGTAGTAATGGGGCACTCttt                                                                                                                                     <  2:113962/236‑1 (MQ=255)                                                                                                                      gccgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCTGAGAAACAGAACGCGCTGCGTGTCGGGC‑ATCTGGCGAAAAATACCCTCCTCCACCGCCAGGTTAAAACACGGGGCGCAAGAGTCAGAGATATGCAGGCGTAATGTGGACATAACGGTTTCGTTTCTTGTAGTACTGGGGCAATCTTTTTtc                                                                                                                                 >  2:545272/1‑251 (MQ=255)                                                                                                                      gccgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc                                                                                                                                 <  1:657649/251‑1 (MQ=255)                                                                                                                            ccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt                                                                                                                           <  1:451492/251‑1 (MQ=255)                                                                                                                             cAGGCGGACGTTATCTTCTTCCATCCGCCGGGTGTTACATTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATATACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt                                                                                                                          >  1:546258/1‑251 (MQ=255)                                                                                                                             cAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTATGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGCAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCCGGTTAAACAACGGGTCGGAAGAGTCAGAGATGAGCAGGCGTAATGTGGCCATACAGATTTCCTTTCTTATTGTTCTGGGACACTCTTTTTTCTCTCttt                                                                                                                          >  2:61466/1‑251 (MQ=255)                                                                                                                                   gACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcct                                                                                                                    <  1:373961/251‑1 (MQ=255)                                                                                                                                    aCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctc                                                                                                                   <  1:231139/251‑1 (MQ=255)                                                                                                                                    aCGTTAGCGTCTTCAATCCGCCGGGTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTAGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑ATCTGGCGAAAAATACACGCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctc                                                                                                                   <  1:352924/251‑1 (MQ=255)                                                                                                                                     cGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctct                                                                                                                  >  1:59702/1‑251 (MQ=255)                                                                                                                                      gatATCTTCTTCCATCAGCCGGGTCTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAACATACACTCATCCACCGCCAGGTTAAACCACGGGTCGGAAGAGTCAGTGCTGAGCAGGCGTTATGGGGAAATAACGATTTCATTTCTTTTACGGATGGTACACTCTTTTTTATCGCTTtcttcctctt                                                                                                                 >  2:272932/3‑251 (MQ=255)                                                                                                                                             tcttcCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                                                                                                                                                    <  2:181668/209‑1 (MQ=255)                                                                                                                                                   agccgccgGTTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACGCCTCCACCGCCATGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGGCACTCTTTTTTCACGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                    <  2:47857/249‑1 (MQ=255)                                                                                                                                                         cgGGTATTACACGCCTGCCACGGGTCCTGCGCGCGACCAACCACTACCG‑GGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGA‑ATCTGGCCAAAAATACACTCCCCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCCCTCTTTTTGCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGc                                                                                              <  1:402453/251‑1 (MQ=255)                                                                                                                                                                 acacTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACa                                                                                     <  1:565540/251‑1 (MQ=255)                                                                                                                                                                    ctctTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGGTTAAcggg                                                                                   >  2:575827/1‑248 (MQ=255)                                                                                                                                                                           cACGGTTTCTTCGCGCGACCAATCACGACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACGCTCCTCCGCCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGATCAGGCGTAATTTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑a                                                                          <  2:59702/251‑1 (MQ=255)                                                                                                                                                                           cACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt                                                                                                                          <  1:150989/205‑1 (MQ=255)                                                                                                                                                                           cACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑GTGACAGGAAggggcg                                                                           >  1:190757/1‑248 (MQ=255)                                                                                                                                                                                gTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                                                                                 >  1:534574/1‑145 (MQ=255)                                                                                                                                                                                gTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACACCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                                                                                 <  2:534574/145‑1 (MQ=255)                                                                                                                                                                                 ttCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGCAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAAGAAGGGCgctttcgt                                                                     >  2:483998/1‑244 (MQ=255)                                                                                                                                                                                 ttCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTTACAAGAAGGGCG‑GTtgctt                                                                    >  1:233386/1‑250 (MQ=255)                                                                                                                                                                                 gtCTGCGCGCGACCAATCACTACCG‑TGTCGGCATGGCGCCAGAGAAACAGAACGCCCTGCGTGGCGGGC‑ATCTGGCTAAAAATACACGCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGTTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATtgctg                                                                    <  2:417763/250‑1 (MQ=255)                                                                                                                                                                                           gACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGt                                                           >  1:609607/1‑250 (MQ=255)                                                                                                                                                                                            aCCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGGG‑GTTGCTGCCAGCGGGtac                                                         >  1:361877/1‑249 (MQ=255)                                                                                                                                                                                             acctaacacgaccggggcGGCATTGCGCCAGAGAAACAGAACGGGCTGCGTGGCGGGA‑ATCGGGCTAAAAATACACCCCGCCACCGCCAGTGTAAAACACGGGTCGTAAGAGTCAGAGATGAGCAGGCGGAAGGTGGACATAACTATTTCCTTTCTTGTAGGAATGGGTTACGCTTTTTTCTCGCTTTCTTCCTCTTCCGGCGCCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGtac                                                         <  2:647858/234‑3 (MQ=255)                                                                                                                                                                                               aatcactacCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTTACAGGGAGGGCGGATT‑CTGCCAGCggggaagtt                                                      >  1:487277/5‑245 (MQ=255)                                                                                                                                                                                                     aacCG‑TGTCGGCATTGCGCCAGAAAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACCCCCCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCTG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTACCCt                                               <  2:361877/250‑1 (MQ=255)                                                                                                                                                                                                             ccGGCATTGAGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCTCCGTCAGGTGAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTATTAATGGGTCACTCTTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCGGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTCC‑GTTTACCCTGTagcag                                        <  2:320700/250‑1 (MQ=255)                                                                                                                                                                                                                 cATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCAACGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGACACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAATGGGG‑GTTGCGGCCAGGGGGTTCAGTTT‑CCCTGTaggaacagg                                    >  1:114783/1‑244 (MQ=255)                                                                                                                                                                                                                    tGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGG‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTACCCTGTAGCAGCGTGCg                                  <  2:59368/250‑1 (MQ=255)                                                                                                                                                                                                                      cgcCAGAGAAACAGACCGCTCTGCGGGGCGTGC‑ATCTGGCGAAAAATACACGCCTCCACCTCAAGGTTAAACCACGGGTCGGAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACTATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTGTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTACCCTGTAGCAGCGTGCGGGt                               <  2:8664/251‑1 (MQ=255)                                                                                                                                                                                                                      cgcCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGGAC‑GGTTTCCCTTTAGCAACGTGCgggg                               >  1:517528/1‑250 (MQ=255)                                                                                                                                                                                                                          agagAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTACCCTGTAGCAGCGTGCGGGTCAGc                           <  1:29814/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTTCCCTTTAGCAGCGTGCGGGTCAGCACTacgc                    >  1:410702/1‑249 (MQ=255)                                                                                                                                                                                                                                     aCGCGCGGCGTGGCGGGC‑AGCGGGCGAAAAATACACTCCCCCACCGCCATGTTAAACCGTGGGTCGTAAGAGTCAGAGATGAGCAGTCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAGGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGTTAC‑GTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGAt                <  2:97197/251‑1 (MQ=255)                                                                                                                                                                                                                                      cgcgGTGCTTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCTACGCCAGGTTAAACCTCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAATTCCTTTCTTGTAGTAATGGGTCACTCTTTGTTCCCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCTG‑TTACCAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTTCCCTGTAGCAGCGTGCGGGTCAGctttatgcagatt               <  2:609607/251‑13 (MQ=255)                                                                                                                                                                                                                                       gcgcTGCGTGGCGGGC‑ATCTGGCGAAAAATAAACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTg              <  1:96171/251‑1 (MQ=255)                                                                                                                                                                                                                                        cgcTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTTCTGCCAGCGGGTAC‑GTTTACCCTGTAGCAGCGGGCGGGTCAGCCCTACGCCGATTGc             >  1:354667/1‑251 (MQ=255)                                                                                                                                                                                                                                               tGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTaa                                                                                       <  1:400832/172‑1 (MQ=255)                                                                                                                                                                                                                                                   gggC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTTACAGGAAGGGCG‑ATTGCTGCCAGCGGGTAC‑GTTTACCCTGTGGCAGCGTGCGGGTCAGCACTACGCCGCTTGCCAGTGAAagcc  >  1:432368/1‑250 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        CAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCG‑ATTGCTGCCAGCGGGTGC‑GTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCA  >  NC_000913/4623791‑4624281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑