Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,623,983 T→C V45V (GTA→GTG)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,623,9830TC96.9% 289.2 / ‑0.8 96V45V (GTA→GTG) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (39/54);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

TTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGATTGCTGCCAGCGGGTGC  >  NC_000913/4623736‑4624227
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       
ttttCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATcact                                                                                                                                                                                                                                                      >  1:186391/1‑251 (MQ=255)
  ttCACCCCCAGATCGTTACGCCCGGACGCTTCGGCGCTGCCGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATcacta                                                                                                                                                                                                                                                     <  2:135074/250‑1 (MQ=255)
   tCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGACGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTCCACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTAcc                                                                                                                                                                                                                                                   >  2:29814/1‑251 (MQ=255)
    caccacCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCg                                                                                                                                                                                                                                                  >  1:417763/1‑251 (MQ=255)
    caccacCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGGGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTAcc                                                                                                                                                                                                                                                   <  1:612099/250‑1 (MQ=255)
               cGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCAtt                                                                                                                                                                                                                                       <  1:306117/250‑1 (MQ=255)
                gTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTgc                                                                                                                                                                                                                                     <  1:678330/251‑1 (MQ=255)
                  tACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCAAAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTgcgc                                                                                                                                                                                                                                   >  1:47857/1‑251 (MQ=255)
                      cccGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGCCGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTCTTCCACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGccgg                                                                                                                                                                                                                                >  2:184275/2‑248 (MQ=255)
                         ggACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTAATCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCCCGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAgaga                                                                                                                                                                                                                             >  2:476665/1‑250 (MQ=255)
                               ttCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCCCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCAGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTAACG‑GTTCGGCATTGCGCCAAAGAAACaaa                                                                                                                                                                                                                       >  2:595700/1‑248 (MQ=255)
                                   gcgcTGACGCCAAGCGCGTTCAGTGCATTGAGCACAATCGTCGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAATGTAAAGCATGTATGGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTAGTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAAcgcg                                                                                                                                                                                                                  <  1:243274/251‑1 (MQ=255)
                                     gcTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCt                                                                                                                                                                                                                >  1:296500/1‑251 (MQ=255)
                                      cTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGTCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGATTCTGCGCGCGCCCAATCACTACCG‑TGTCGGCCTTGCGCCAGCGAAACAGAAcgcgatg                                                                                                                                                                                                               >  2:489380/1‑248 (MQ=255)
                                           gctaaGCGCGTTAAGCGCAGTGAGCACTATCGACGGGGAGAGGGTTTTATGGTACTCCCGCTGGCCAGCCAGAAAGGTAAAGCAGGGATGGACGAGATCGTGGAACCCCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTAGCTTCTTCCATCCGCCGGGTATTACACGCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTgg                                                                                                                                                                                                          <  2:248254/247‑1 (MQ=255)
                                           gccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGAATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCAACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCCGAGAAACAGAACGCGCTGCGTgg                                                                                                                                                                                                          >  1:629969/5‑251 (MQ=255)
                                            ccagtagcgTTCAGCGCATTGGTCATGAGCGACGTGGGGAGGGTTTTACCGTACTCCGGCTTGCCATCCATAAAGGTTAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTAC‑TTCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACTGTTTCTGCGCGCGTCCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCGGCGTGGc                                                                                                                                                                                                         <  2:666201/245‑1 (MQ=255)
                                               aGCGCGTTCAGCGCAGTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCggg                                                                                                                                                                                                      <  1:71047/251‑1 (MQ=255)
                                                  gcgTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑At                                                                                                                                                                                                  <  1:339185/251‑1 (MQ=255)
                                                   cgTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGCACGCGCTGCGTGGCGGGC‑Atc                                                                                                                                                                                                 >  1:148634/1‑250 (MQ=255)
                                                      tCAGCGCAGTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑Atctg                                                                                                                                                                                               <  2:168480/250‑4 (MQ=255)
                                                       cAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGc                                                                                                                                                                                             <  2:186391/251‑1 (MQ=255)
                                                              ttGAGCACAAACGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGAATTGCCGGGATCGTGGAAAACCGCGCCGCAACCGCAAC‑TGCGCCGTGCCAGGCGGGCGTTATCTACTTCCATCCGCCGGGGCTTAACCTCTTTCACCGAGTTCGGCGCGCGACCATTCAC‑CCCGATGTAGGCATTCCGCCAGAGAACAAGAACGAGCTGCGTG‑CGGGCCACTTGACGaaaaa                                                                                                                                                                                       >  2:477607/1‑250 (MQ=255)
                                                                    acaATAGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACAAATCACTCCCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGCCGGGC‑CTCTGGCGAAACATACActc                                                                                                                                                                                >  2:574751/1‑251 (MQ=255)
                                                                     caATCGACGTGGAGATGGTTGTACCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGAGCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACActcc                                                                                                                                                                               <  1:131759/251‑2 (MQ=255)
                                                                      aaTCGGCGTGGAGATGGTTTTATCGTACCCCGGTGTGCCAGCCATAAAGGTAAAGCAGGTAGTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACGCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATGCActcct                                                                                                                                                                              <  2:498080/251‑3 (MQ=255)
                                                                      aaTCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACActcct                                                                                                                                                                              <  1:329840/251‑3 (MQ=255)
                                                                         cGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAAATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCTTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑GTTCGGCTTTGCGCCAGGGAAACAGAACGCGCTGCGTGGCGGGC‑ACCGGGCGAAAAATACACtcctcc                                                                                                                                                                            >  2:238040/1‑250 (MQ=255)
                                                                         cGACGTGGAGATGGTTTTATCGTACGCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTAGCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCa                                                                                                                                                                           <  1:109943/251‑1 (MQ=255)
                                                                                        tttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAc                                                                                                                                                             <  1:611465/250‑1 (MQ=255)
                                                                                        tttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAc                                                                                                                                                             <  1:224981/250‑1 (MQ=255)
                                                                                         ttctcgttccccGGTTTGCCAGCCATAAAGGTAAAGCAGGTATGGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGGGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACTCTCCTCCACCGCCAGGTTAAAcc                                                                                                                                                            <  2:485690/240‑1 (MQ=255)
                                                                                         ttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAccc                                                                                                                                                           >  1:464672/1‑250 (MQ=255)
                                                                                          tATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCAc                                                                                                                                                          >  1:243935/1‑251 (MQ=255)
                                                                                                  tCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGCCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGt                                                                                                                                                   >  1:248500/1‑250 (MQ=255)
                                                                                                     ggatggccagacaAAAAGGAAAAGCAGGTATAGCAAAGAGCGTGGAACCACGCGCCGACACCGCTAC‑TGCGCCTTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATGACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCAAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAaga                                                                                                                                               <  2:295172/246‑1 (MQ=255)
                                                                                                         tGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTc                                                                                                                                            >  1:19881/1‑250 (MQ=255)
                                                                                                           ccagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATCCACTCCTCCACCGCCCGGTTAAACCACGGGTCGGAAGAGTccg                                                                                                                                          >  2:231139/1‑248 (MQ=255)
                                                                                                            cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCagag                                                                                                                                        <  2:212519/251‑1 (MQ=255)
                                                                                                            cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCTGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGACAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCag                                                                                                                                          >  1:472910/1‑249 (MQ=255)
                                                                                                              gccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGAt                                                                                                                                      <  1:669156/251‑1 (MQ=255)
                                                                                                               ccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATg                                                                                                                                     >  1:312729/1‑251 (MQ=255)
                                                                                                               ccaTAAAGGGAAAGCAGGTATTGCCGAGATCGTGTAACACCCCGCCGCCACCGCTACGTG‑TCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGTTATTACACTCTTTCCCCGGGTTCTGCGCGCGACCAATCACTCCCG‑TGTCGGCATTGCGCCAGAGAAACAGAACTCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCTCCCCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATg                                                                                                                                     <  2:652859/251‑1 (MQ=255)
                                                                                                                caTAAAGGTAAAGCAGGTATGGCCGAGATCGTGGAACACCGCGCCGCCCCCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGa                                                                                                                                    <  1:117934/251‑1 (MQ=255)
                                                                                                                   aaaGGTAAAGCAGGGATTGCCGTAAGCGTGGAACCCCGCGCCGCCACAGGTTC‑TGCTCCGTGCCAGTCGGCAGTTATCGTCTTCCACCCGCAGGGGATTACTCGCTTTCCACGGGTCCTGCGCGCGACCAATCACGTCCG‑TGCCGGCATTGCGCCAGAGAACCAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGCGATGAGCa                                                                                                                                 <  2:506825/251‑1 (MQ=255)
                                                                                                                    aaGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGc                                                                                                                                  >  1:222499/1‑249 (MQ=255)
                                                                                                                     aGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAgg                                                                                                                               <  1:184275/251‑1 (MQ=255)
                                                                                                                     aGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAgg                                                                                                                               <  1:238040/251‑1 (MQ=255)
                                                                                                                       gTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCGCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCg                                                                                                                             <  1:382994/251‑1 (MQ=255)
                                                                                                                        tAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGCGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTCAGAGTCAGGGCTGAGCAGGCg                                                                                                                             >  2:139829/1‑250 (MQ=255)
                                                                                                                         aaaGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                                                                                            <  1:186437/250‑1 (MQ=255)
                                                                                                                          aaGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGCAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTa                                                                                                                           >  1:320700/1‑250 (MQ=255)
                                                                                                                           aGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                         <  1:661649/251‑1 (MQ=255)
                                                                                                                            gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                                                                                        >  1:251036/1‑251 (MQ=255)
                                                                                                                            gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                                                                                        <  1:6609/251‑1 (MQ=255)
                                                                                                                            gCAGGTATTGCCGAGATCGTGGAACACCGCGACGCCACCGCTAC‑TGAGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCACCAACGCCAGGTTAAACCACGGGACGTAAGAGTCAGAGATGAGCTGGCGTAAtg                                                                                                                        >  1:272528/1‑251 (MQ=255)
                                                                                                                              aGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                                                                                      >  1:375496/1‑251 (MQ=255)
                                                                                                                                  aTTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAc                                                                                                                   <  1:364834/250‑1 (MQ=255)
                                                                                                                                    tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAt                                                                                                                 >  1:46955/1‑250 (MQ=255)
                                                                                                                                     gCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGATATATTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACAACGGGGCGTAAGAGTCAGCGCAGAGCCGGCGTAATGTGGACATa                                                                                                                >  2:617043/1‑250 (MQ=255)
                                                                                                                                     gCCGAGATCGGGGAACACCGCGGCGCCACCGCTAC‑GGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATa                                                                                                                <  1:472482/250‑1 (MQ=255)
                                                                                                                                      ccGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATGGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAc                                                                                                              <  1:489599/251‑1 (MQ=255)
                                                                                                                                         ggaTCGTGGAACCCCGCGCCGCCACCGCTAC‑GGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATATCGAt                                                                                                           <  2:296500/250‑1 (MQ=255)
                                                                                                                                         ataTCGGGGAACCCCGCGCCTCCACCGCTAC‑TGCGCCGCGCCAGGCGTACGGTATCTTCGTCCTGCCGCCGGGTATTACACTCGTTCCACGGGTTCGGCGCGCGACCAAGCTCTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCTTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACTGGTTGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGaa                                                                                                           <  2:83547/249‑2 (MQ=255)
                                                                                                                                         agaTCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                                                                                           <  1:476665/251‑1 (MQ=255)
                                                                                                                                               tGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                                                                                     >  1:5642/1‑251 (MQ=255)
                                                                                                                                                gtaacaccctgccgccACCGCTAC‑TTCGCCGTGCCCGGCGGACTGTATCTGCTTCCACCCGCCGGTTTTTACTCTCTTGCCACGGGTTCTGCGCGCGACCTAGCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGTCGGTA‑ATCGGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                    <  2:351187/241‑1 (MQ=255)
                                                                                                                                                                 ccGCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCa                                                                                   <  1:577112/251‑1 (MQ=255)
                                                                                                                                                                   gCTAC‑TGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                                                                                 <  1:214018/251‑1 (MQ=255)
                                                                                                                                                                    gctccggcgccggtccAGGCTGGCGTTATCCTCTTCCCCCCGCCGGGTATTACTCTCTTTCCACCGGTTCTGCGCGCGACCAATCTCCACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCCCCGCCAGGTTAAACCACGGGTAGTAAGAGTCAGAGATGAGCAGGCGTTATCTTGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                                                                                 <  1:272564/237‑1 (MQ=255)
                                                                                                                                                                       acggcgccgtgccAGGCGGACGTTATCCTCTTCCATCCGCCGGGTATTACTCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCAAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                                                                                <  1:16325/246‑1 (MQ=255)
                                                                                                                                                                        ccgcgccgtgccAGGCTGACGTGATCTTCTTCCCTCCGCCGGGTATTACACGCTTTCCACGGGTTCGGCGCGCGACCAATCACTCCCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCGTGTAGTAATGGGTCACTCttt                                                                             <  2:338183/249‑1 (MQ=255)
                                                                                                                                                                          cggccgtgcatgccGGACGTTATCTGCCTCCATCCGCCGGTTATTACACGCTTTCCACTGGTTCGGCGCGCGACCAATCACTACCG‑TGCCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑AGCTGGCGAAAAATTCACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTTATGTGGACAGAACGATTTCCTTTCTTGTAGTAATGGGGCACTCttt                                                                             <  2:113962/236‑1 (MQ=255)
                                                                                                                                                                            gccgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCTGAGAAACAGAACGCGCTGCGTGTCGGGC‑ATCTGGCGAAAAATACCCTCCTCCACCGCCAGGTTAAAACACGGGGCGCAAGAGTCAGAGATATGCAGGCGTAATGTGGACATAACGGTTTCGTTTCTTGTAGTACTGGGGCAATCTTTTTtc                                                                         >  2:545272/1‑251 (MQ=255)
                                                                                                                                                                            gccgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc                                                                         <  1:657649/251‑1 (MQ=255)
                                                                                                                                                                                  ccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt                                                                   <  1:451492/251‑1 (MQ=255)
                                                                                                                                                                                   cAGGCGGACGTTATCTTCTTCCATCCGCCGGGTGTTACATTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATATACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt                                                                  >  1:546258/1‑251 (MQ=255)
                                                                                                                                                                                   cAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTATGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGCAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCCGGTTAAACAACGGGTCGGAAGAGTCAGAGATGAGCAGGCGTAATGTGGCCATACAGATTTCCTTTCTTATTGTTCTGGGACACTCTTTTTTCTCTCttt                                                                  >  2:61466/1‑251 (MQ=255)
                                                                                                                                                                                         gACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcct                                                            <  1:373961/251‑1 (MQ=255)
                                                                                                                                                                                          aCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctc                                                           <  1:231139/251‑1 (MQ=255)
                                                                                                                                                                                          aCGTTAGCGTCTTCAATCCGCCGGGTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTAGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGC‑ATCTGGCGAAAAATACACGCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctc                                                           <  1:352924/251‑1 (MQ=255)
                                                                                                                                                                                           cGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctct                                                          >  1:59702/1‑251 (MQ=255)
                                                                                                                                                                                            gatATCTTCTTCCATCAGCCGGGTCTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAACATACACTCATCCACCGCCAGGTTAAACCACGGGTCGGAAGAGTCAGTGCTGAGCAGGCGTTATGGGGAAATAACGATTTCATTTCTTTTACGGATGGTACACTCTTTTTTATCGCTTtcttcctctt                                                         >  2:272932/3‑251 (MQ=255)
                                                                                                                                                                                                   tcttcCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                                                                                            <  2:181668/209‑1 (MQ=255)
                                                                                                                                                                                                         agccgccgGTTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACGCCTCCACCGCCATGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGGCACTCTTTTTTCACGCTTTCTTCCTCTTCCGGCACCGGttt                                            <  2:47857/249‑1 (MQ=255)
                                                                                                                                                                                                               cgGGTATTACACGCCTGCCACGGGTCCTGCGCGCGACCAACCACTACCG‑GGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGA‑ATCTGGCCAAAAATACACTCCCCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCCCTCTTTTTGCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGc                                      <  1:402453/251‑1 (MQ=255)
                                                                                                                                                                                                                       acacTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACa                             <  1:565540/251‑1 (MQ=255)
                                                                                                                                                                                                                          ctctTTCCACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGGTTAAcggg                           >  2:575827/1‑248 (MQ=255)
                                                                                                                                                                                                                                 cACGGTTTCTTCGCGCGACCAATCACGACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACGCTCCTCCGCCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGATCAGGCGTAATTTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGa                   <  2:59702/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt                                                                  <  1:150989/205‑1 (MQ=255)
                                                                                                                                                                                                                                 cACGGGTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑GTGACAGGAAggggcg                   >  1:190757/1‑248 (MQ=255)
                                                                                                                                                                                                                                      gTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                         >  1:534574/1‑145 (MQ=255)
                                                                                                                                                                                                                                      gTTCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACACCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                         <  2:534574/145‑1 (MQ=255)
                                                                                                                                                                                                                                       ttCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGCAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAAGAAGGGCgctttcgt             >  2:483998/1‑244 (MQ=255)
                                                                                                                                                                                                                                       ttCTGCGCGCGACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTTACAAGAAGGGCGGTtgctt             >  1:233386/1‑250 (MQ=255)
                                                                                                                                                                                                                                       gtCTGCGCGCGACCAATCACTACCG‑TGTCGGCATGGCGCCAGAGAAACAGAACGCCCTGCGTGGCGGGC‑ATCTGGCTAAAAATACACGCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGTTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGATtgctg             <  2:417763/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 gACCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGATTGCTGCCAGCGGGt    >  1:609607/1‑250 (MQ=255)
                                                                                                                                                                                                                                                  aCCAATCACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGGGGTTGCTGCCAGCGGGtac  >  1:361877/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       
TTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAC‑TGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCG‑TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGC‑ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGG‑TTAACAGGAAGGGCGATTGCTGCCAGCGGGTGC  >  NC_000913/4623736‑4624227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑