Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,623,473 G→A R215R (CGC→CGT)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,623,4730GA98.6% 226.2 / NA 73R215R (CGC→CGT) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/34);  minor base C (0/1);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTC‑CACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGT‑AGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCC  >  NC_000913/4623248‑4623709
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              
cggcgCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAgg                                                                                                                                                                                                                       >  1:514301/1‑251 (MQ=255)
 ggcgCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGc                                                                                                                                                                                                                      >  1:286843/1‑251 (MQ=255)
         gTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGtt                                                                                                                                                                                                               >  1:240777/1‑250 (MQ=255)
            gAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATgg                                                                                                                                                                                                           <  1:96869/251‑1 (MQ=255)
              ggCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGcc                                                                                                                                                                                                         >  1:320403/1‑251 (MQ=255)
                  gTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTc                                                                                                                                                                                                       <  1:286819/249‑1 (MQ=255)
                   ccGGTAAATACCCGGGCGCGGGTGATATGTCCTTTTTCAACGACGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCCTTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGCCCGTGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGTCTCGCa                                                                                                                                                                                                    <  2:673131/250‑1 (MQ=255)
                        aaaCACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTCCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAACCCt                                                                                                                                                                                               >  1:254737/1‑251 (MQ=255)
                         aaCACCTGGGCGCGGGTGAGATGCCCTTTGTCAACTGCGAAATGCAGTCCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGGTTCGGCGAAGTTTGGCTAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑GACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTAGGGCCTCGCTAACCTg                                                                                                                                                                                              <  2:48782/251‑1 (MQ=255)
                                  gagagggTGATATGGACTTTTGAAACGTCTAGAAGCAGTTCCACGCCGACCCAGCAAAAGCGTGCAGCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGGTCCATTCCCAGCTCCTCTGACTGGAGAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTGCCGCTTC‑TACACGCTCGCCATAAGGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGt                                                                                                                                                                                     <  2:212243/247‑1 (MQ=255)
                                     cgGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGAt                                                                                                                                                                                  >  1:171663/1‑251 (MQ=255)
                                        gTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGTTTATGGCCTCGCAAACCTGCTCATGGGTGATccc                                                                                                                                                                               <  2:205669/251‑1 (MQ=255)
                                         tGATATGGCCTTTTTCAACGTAGAAATGCAGTTTCACGCCGCCCCAGCTAAAGCGTTCTTCCAGCAGATGCGAGACTGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCCAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGTTGATTTCCGCTTC‑TACACGCTCGCCCTAATGGACGCAAAAGGCCTTGTTTTTGGCCTCGCAACCCTGCTCATGTGTGCTccac                                                                                                                                                                              >  2:96547/1‑249 (MQ=255)
                                                       taaacgtctgaaTGCAGTTCCACGTCGCTTCAGCTAATGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATCCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGAGTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGTTTTGGTTATGGTCTCGCAAACCTGCTCATGGGTGATCCCTGGCAGCAGCTCgt                                                                                                                                                                 <  1:546319/240‑2 (MQ=255)
                                                       tCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACAAGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCgg                                                                                                                                                                 <  1:546301/250‑1 (MQ=255)
                                                        cAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTg                                                                                                                                                               <  1:221577/251‑1 (MQ=255)
                                                            gTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGt                                                                                                                                                           >  1:641912/1‑251 (MQ=255)
                                                                aaaTGCCGTCCCACGCCGCCCCAGCTAAAGCTTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTGTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTGC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCGCGGTGAGGTTGGt                                                                                                                                                       <  2:439276/251‑1 (MQ=255)
                                                                 aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAACGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCGCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCACACCTGCTCATGGGTGATCCCCGGCAGCAGCACggggcgggtggtc                                                                                                                                                      >  2:224560/1‑240 (MQ=255)
                                                                 aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTc                                                                                                                                                      >  1:46376/1‑251 (MQ=255)
                                                                 aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTc                                                                                                                                                      >  1:371130/1‑251 (MQ=255)
                                                                 aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTc                                                                                                                                                      >  1:564699/1‑251 (MQ=255)
                                                                     cAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCAcgc                                                                                                                                                  >  1:405487/1‑251 (MQ=255)
                                                                       gTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGCAAAAGGCCTTGGTTATGGCTTCGCAAACCTGCTCCTGGGTGATCCCCGGCAGCAGCCCGGTGGGGTTGGTCCCGCgg                                                                                                                                                >  2:246815/1‑251 (MQ=255)
                                                                           cACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAAc                                                                                                                                            <  1:253548/251‑1 (MQ=255)
                                                                              gccgccCCAGCTAAAGTGTTCAACCAGCAGATGCGAGAATGCCGGTGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAATGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgca                                                                                                                                         <  2:514301/251‑3 (MQ=255)
                                                                                cgccCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcact                                                                                                                                       <  1:376925/251‑5 (MQ=255)
                                                                                cgccCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcact                                                                                                                                       <  1:468321/251‑5 (MQ=255)
                                                                                   cccatctaaggCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGATCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGCAGTTTGGCAAGTCGGGCGTTTTGTCAGGGGAGCTGATTTCCGCTTCTCA‑ACGCTCGCCATCATGGTCGAACAAGGCCTTGGTTCAGGCCTCACAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGCTGGACCCGCGGGAACgcactg                                                                                                                                      >  2:131759/11‑244 (MQ=255)
                                                                                      agctAAAGCGTTCATCCAGCAGATGCGAGAATGCAGGTGCCTGCCCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAATGGCCTTGGTTATGGCCTCGCAATCCTGCTCATGGGAGTTCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcactgagga                                                                                                                                  >  1:153095/4‑241 (MQ=255)
                                                                                        ctAAAGCGTTCATCCAGCAGATGCGAGAATGCAGGCGCCTGACCGAAGATCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTA‑TACACGCTCGCCATATTGGGCGAAAAAGGCCTTGGTTATGGCCTCGCCACACTGCTCATGGGTGATCCCCGGCAGCAGCCCGGGGAGGGTGGTCActagggaccgaactgcggta                                                                                                                                 >  2:543949/2‑230 (MQ=255)
                                                                                          aaaGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AAt                                                                                                                              <  1:246815/249‑1 (MQ=255)
                                                                                          aaaGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGc                                                                                                                            <  1:15805/251‑1 (MQ=255)
                                                                                               gtacctCCAGCAGATGCTAGAAGGCCGGAGCCTGACCGAAGTTCCATTCTCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTc                                                                                                                       <  2:565100/246‑1 (MQ=255)
                                                                                                  cATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTcgcc                                                                                                                    <  2:240777/251‑1 (MQ=255)
                                                                                                     ccAGCAGATGCGAGAATGCCGGAGCCTGACCGCAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTATCCGGGGAGATGATTTCCGATTA‑TACACGCTATACATAATGGGCGCAAAAGGCCTTGGTTATGGCCTCGAATACCAGCTCATGGGTGATCCCCGGCAGTAGCTCGGTGAGACTGGTAACGCGGGAACGCACTG‑CATGAATGCCTTTCgccgcc                                                                                                                 >  2:28774/1‑251 (MQ=255)
                                                                                                      cagcagATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGc                                                                                                                                                                                                                               >  2:163640/1‑141 (MQ=255)
                                                                                                      cagcagATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGc                                                                                                                                                                                                                               <  1:163640/141‑1 (MQ=255)
                                                                                                       agcagATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTTGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCGCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAg                                                                                                               <  2:371130/251‑1 (MQ=255)
                                                                                                          agATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCg                                                                                                                                                                                                      >  2:361687/1‑162 (MQ=255)
                                                                                                          agATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCg                                                                                                                                                                                                      <  1:361687/162‑1 (MQ=255)
                                                                                                             ggCGAGAATGCCGGGGCCTGACCGAAGTTCCTTTCCCAGCTTCTCTGACGGGCAAAGGTTTCGGCGAAGTTGGGCAAGGCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACCCGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGAGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCtt                                                                                                         <  2:66009/250‑1 (MQ=255)
                                                                                                                 agaATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGCTTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCtttttt                                                                                                     >  2:486906/1‑250 (MQ=255)
                                                                                                                      gCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCg                                                                                                 <  1:615894/250‑1 (MQ=255)
                                                                                                                        cGGAGCCTGACCGAAGGTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGAt                                                                                              <  1:87375/251‑1 (MQ=255)
                                                                                                                         ggAGCCTGACCGACGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGACGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCACGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGA‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGAtt                                                                                             >  1:622251/1‑251 (MQ=255)
                                                                                                                         ggAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGAtt                                                                                             >  1:625020/1‑251 (MQ=255)
                                                                                                                             ccTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGCTGATTTACGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGATTATGGCCTAGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTAGCCGCCAGTTTCTTTTTATcagggatgagg                                                                                         >  2:640427/1‑241 (MQ=255)
                                                                                                                               tGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCttg                                                                                                                                                                                                                  >  2:545186/1‑127 (MQ=255)
                                                                                                                               tGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCttg                                                                                                                                                                                                                  <  1:545186/129‑3 (MQ=255)
                                                                                                                                gACCGAAGTACCATTCCCAGCTACTGTGAGGGGCAAAGGTTTCGGGTAAGTTGGGAAAGTCTGGCGTTTTGT‑CCGGGAGATGATTTCCGCTTC‑TACACTCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTAGCCGGATTGAGATAg                                                                                      <  1:496789/250‑1 (MQ=255)
                                                                                                                                     aaGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTGCGGCGAAGTTGGGCAAGTCTGGCGTTTTGTCCGGGTAGATGATTTCCGCTGC‑GACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGGGAGGTTTGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTTAGATAGTTTGc                                                                                 <  2:622251/251‑1 (MQ=255)
                                                                                                                                      aGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAgg                                                                                                                                                            <  1:194969/176‑1 (MQ=255)
                                                                                                                                      aGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAgg                                                                                                                                                            >  2:194969/1‑176 (MQ=255)
                                                                                                                                                           gacgtgCAAAGGTTTCGGCGAAGTTTGGCAAGTTGGGTGTGTTGGCCGGGGAGATGATTGCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCCCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCTGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCAtt                                                            <  2:625020/245‑1 (MQ=255)
                                                                                                                                                                      gTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATACTGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAAGGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGAGAGTTTGCCAAGCGGCTAAGGGCGGCCTTGACC‑AGCAAggg                                               >  2:322347/1‑250 (MQ=255)
                                                                                                                                                                           ggCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCgt                                          >  1:584754/1‑251 (MQ=255)
                                                                                                                                                                           ggCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCCGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCgt                                          >  1:83547/1‑251 (MQ=255)
                                                                                                                                                                           ggCGAAGTTTGGCAAGACTGGCGTTTTGTCCGGGGAGATGCTTTCAGCTTC‑TACACGCTCGCCATACTGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTAGGTGAGGTTGGTACCGCGGGAAAGCACTGAGGT‑AATGCCTTTCGCCGCCAGGTTCTTTTTATCCGGATTGAGATAGTTGGCCCGGCGGCTAAGGTCGGCATT‑TGTCAGCAAGGTGCCgg                                          >  2:538409/1‑250 (MQ=255)
                                                                                                                                                                              gAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAAATGATTTCCGCTTC‑TACACTCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCGGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCgtggt                                       <  2:641912/251‑1 (MQ=255)
                                                                                                                                                                                     ggCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTg                                                           >  1:369649/1‑225 (MQ=255)
                                                                                                                                                                                      gCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGcca                               <  1:619667/251‑2 (MQ=255)
                                                                                                                                                                                       cAAGTCTGTGGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCCCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGccac                              <  2:545451/251‑3 (MQ=255)
                                                                                                                                                                                       cAAGTCTGGCGTTTTGCCCGGGGCGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCTGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGctcc                              >  2:382994/1‑248 (MQ=255)
                                                                                                                                                                                             tGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCtt                        >  1:363176/1‑251 (MQ=255)
                                                                                                                                                                                             gggCGTTTGGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTGGGTCACGCGGGAACGCACTGATGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGTCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCtt                        <  1:157567/250‑1 (MQ=255)
                                                                                                                                                                                               gCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTTGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCTTTg                      >  2:620229/1‑251 (MQ=255)
                                                                                                                                                                                                cGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCTTTgg                     >  1:390881/1‑251 (MQ=255)
                                                                                                                                                                                                     ggtcgggggggATGATTTCCGCTTC‑TACACTGTCGACATAATGGGCGAAAAAGGCCTTGGTTAGGGCCTCGCAATCCTGCTCATGGGTGATCCCCGGCAGCAGCGCGGTGAGGTTGGTCACGCGGGAACGCTCGGAGGT‑AATTCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTGGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTc                 <  2:584754/245‑1 (MQ=255)
                                                                                                                                                                                                      gtccGGGGAGATGATTTCAGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGCTTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGCGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTcgc               >  2:109943/4‑251 (MQ=255)
                                                                                                                                                                                                           gggggttgatTTCCGCGTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTGATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGGGGTGGAAGCCACGATCTTTGGGTTCTCGATAg          <  2:286843/245‑1 (MQ=255)
                                                                                                                                                                                                               gatgatTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCg      >  1:666201/1‑251 (MQ=255)
                                                                                                                                                                                                                   atTTCAGCTTC‑TACACGCGCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACAGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGGTTGAGATAGTTTGCCAGGCGGCTACTGTCGGCATTGAGC‑AGCAAGGTGCCGTGGTGGACGCCACGATCTTTGCGTTCGCGATAGGCCGaacc  >  1:73672/1‑248 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              
CGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTC‑CACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGT‑AGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCC  >  NC_000913/4623248‑4623709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑