| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,623,404 | G→A | R238R (CGC→CGT) | lplA ← | lipoate‑‑protein ligase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,623,404 | 0 | G | A | 96.2% | 230.3 / ‑4.0 | 78 | R238R (CGC→CGT) | lplA | lipoate‑‑protein ligase A |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (34/41); total (35/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTC‑CACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGC > NC_000913/4623157‑4623631 | ccGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACgg < 1:345929/251‑1 (MQ=255) tCAACCAACAGCGCTTCGCACTCTGGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGTCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCAACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGCGCCTGACCGAATTTCGATTCCCAGCTACTCTGACGGGCAAAGGt > 2:221577/1‑251 (MQ=255) aaccaacAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAgggtt > 1:381111/1‑248 (MQ=255) ccaccAGCCCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGTGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCTGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCAGCCAGCAGATGCGAGAAGGCCGGAGCCTGACCGAAGTTCCATTCCCAGTTATTCTGACGGGCAAATGTTTCg < 2:269690/247‑1 (MQ=255) acAGCGCTGCGCACTCGGGTTGCAGCATACCCGCGCGGGACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCATCCAGCGGCGCGGGGTTGAGGCTGTCGGTAACCACCTGGGCGCTGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAACTAAAGCGTTCATCCAGCATATGCGAGAATGCCGTGGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCg < 2:52185/251‑1 (MQ=255) cgtCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGttt < 1:361422/249‑1 (MQ=255) tCGCACTCGTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGttt < 2:283879/249‑1 (MQ=255) cGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTgg < 1:267694/250‑1 (MQ=255) taCTCCTGTTGCAGCATATCTGCGCGGTGCAAGCTGCCTTGCAGTCGTCCGCCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTGAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGCAAAGCAGTTCCACGTCACACCAGCTAAAGCGTTCATCCAGCAGATGAGAGACTGCCGGAGCCTGCCCTAATATCCATTCCCGGCTCCTCTGGCGGGCAAAGGTGTCGGCgtcgtttttgt > 2:21174/2‑240 (MQ=255) tGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTg < 2:155507/250‑1 (MQ=255) gcggatccgcgcGGTTCAGGCAGCATTGCAGCAGGCCGGCGAGGGCTGCCAGCGGCGCGTGGTTGAGGCTGTCGGTGAACACCTGGGCGCGGGTGATATGGCCTGTTTCAACGTCGAAAGGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCAGCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTg < 2:191076/243‑1 (MQ=255) tatCCGCGCGGTCCAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAAATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAATCTGGCGTTTTGtcc > 2:376925/1‑249 (MQ=255) acgggCAGCCTTGCAGTCGCCCGGCGAGGGCTGCCAGCGGCGCGTGGTGGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCGTTTTCAACGTCGAAAGTCAGGTCCAGGCCGCCCCATCGATAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCGAACCGAAGTTCCAGTCCCAGCTACCCTGATGCGCAAAGGTGGCGGCGAATTTTGGCAAGTCTGGCGTTTTGTCCGGGGAgatgat < 1:32171/247‑1 (MQ=255) aGGCAGCCTTGCAGTCTCCCGGCGCGGGCTTCGAGCGGCGCGGGGTTGAGGCTGTCGGGAAACACCCGGGCGCGGGTGATATGGCCGTTTTCAACGTCGAAATGCAGGTACACGCCGCCCCAGCTAAATCGTGCAACCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCATCGACTCTGAAGGGCAAAGGTTTCGGCGAAGTGTGGCAAGTCTGGCGGTTTTTCCGGGGAGTTGAttta < 2:421071/251‑2 (MQ=255) aGGCAGCCTTGCAGGCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTc < 2:278554/251‑1 (MQ=255) aGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTAATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCt > 1:94912/1‑251 (MQ=255) gcaggcgcccGGCGTGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACGCCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTctaca < 2:225313/243‑5 (MQ=255) cAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTctacac > 1:338344/1‑246 (MQ=255) cAGTCGCCCGGCGAGGGCCTCCAGCGGCGGTGGGTTGAGGCTGTCGGTATACTCCCTGGCGCGGGTGATATGTCTTCTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAGGCGTTCTTCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCTGCGAAGTTTGGCAAGTCGGGCGTTTTGTCCGGTGAGATGATTTCCTCTTctacac < 2:295484/251‑6 (MQ=255) cgccGAGGGCTTCCAGAGGCGCGTGGATGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGACATGCAGTTCCACGCCGCACCAGCTAAAGCGTTCATCCAGCAGATGCGCGAATGCCGGAGCCTGACCGCAGTTCAATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGATTGGCAAGTATAGCGTATTGTCCGGGGAGATGATTTCCGcatctacacacccgcc > 2:91461/4‑235 (MQ=255) ggCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGttt > 2:513445/1‑185 (MQ=255) ggCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGttt < 1:513445/185‑1 (MQ=255) cggcgCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAgg > 1:514301/1‑251 (MQ=255) ggcgCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGc > 1:286843/1‑251 (MQ=255) gTTGATGCTGTAGGTAAACACCTGGGCGCGGGAGATATGGCCTTTTTCAACGTCGACATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCCTTCCCAGATACTCTGACGGGCAAGGGTTTCGGca > 2:378471/1‑165 (MQ=255) gTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCg < 1:378471/166‑1 (MQ=255) gTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGtt > 1:240777/1‑250 (MQ=255) gAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATgg < 1:96869/251‑1 (MQ=255) ggCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGcc > 1:320403/1‑251 (MQ=255) gTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTc < 1:286819/249‑1 (MQ=255) ccGGTAAATACCCGGGCGCGGGTGATATGTCCTTTTTCAACGACGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCCTTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGCCCGTGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGTCTCGCa < 2:673131/250‑1 (MQ=255) aaaCACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTCCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAACCCt > 1:254737/1‑251 (MQ=255) aaCACCTGGGCGCGGGTGAGATGCCCTTTGTCAACTGCGAAATGCAGTCCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGGTTCGGCGAAGTTTGGCTAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑GACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTAGGGCCTCGCTAACCTg < 2:48782/251‑1 (MQ=255) gagagggTGATATGGACTTTTGAAACGTCTAGAAGCAGTTCCACGCCGACCCAGCAAAAGCGTGCAGCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGGTCCATTCCCAGCTCCTCTGACTGGAGAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTGCCGCTTC‑TACACGCTCGCCATAAGGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGt < 2:212243/247‑1 (MQ=255) cgGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGAt > 1:171663/1‑251 (MQ=255) gTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGTTTATGGCCTCGCAAACCTGCTCATGGGTGATccc < 2:205669/251‑1 (MQ=255) tGATATGGCCTTTTTCAACGTAGAAATGCAGTTTCACGCCGCCCCAGCTAAAGCGTTCTTCCAGCAGATGCGAGACTGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCCAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGTTGATTTCCGCTTC‑TACACGCTCGCCCTAATGGACGCAAAAGGCCTTGTTTTTGGCCTCGCAACCCTGCTCATGTGTGCTccac > 2:96547/1‑249 (MQ=255) taaacgtctgaaTGCAGTTCCACGTCGCTTCAGCTAATGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATCCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGAGTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGTTTTGGTTATGGTCTCGCAAACCTGCTCATGGGTGATCCCTGGCAGCAGCTCgt < 1:546319/240‑2 (MQ=255) tCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACAAGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCgg < 1:546301/250‑1 (MQ=255) cAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTg < 1:221577/251‑1 (MQ=255) gTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGt > 1:641912/1‑251 (MQ=255) aaaTGCCGTCCCACGCCGCCCCAGCTAAAGCTTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTGTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTGC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCGCGGTGAGGTTGGt < 2:439276/251‑1 (MQ=255) aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAACGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCGCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCACACCTGCTCATGGGTGATCCCCGGCAGCAGCACggggcgggtggtc > 2:224560/1‑240 (MQ=255) aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTc > 1:371130/1‑251 (MQ=255) aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTc > 1:564699/1‑251 (MQ=255) aaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTc > 1:46376/1‑251 (MQ=255) cAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCAcgc > 1:405487/1‑251 (MQ=255) gTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGCAAAAGGCCTTGGTTATGGCTTCGCAAACCTGCTCCTGGGTGATCCCCGGCAGCAGCCCGGTGGGGTTGGTCCCGCgg > 2:246815/1‑251 (MQ=255) cACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAAc < 1:253548/251‑1 (MQ=255) gccgccCCAGCTAAAGTGTTCAACCAGCAGATGCGAGAATGCCGGTGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAATGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgca < 2:514301/251‑3 (MQ=255) cgccCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcact < 1:376925/251‑5 (MQ=255) cgccCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcact < 1:468321/251‑5 (MQ=255) cccatctaaggCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGATCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGCAGTTTGGCAAGTCGGGCGTTTTGTCAGGGGAGCTGATTTCCGCTTCTCA‑ACGCTCGCCATCATGGTCGAACAAGGCCTTGGTTCAGGCCTCACAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGCTGGACCCGCGGGAACgcactg > 2:131759/11‑244 (MQ=255) agctAAAGCGTTCATCCAGCAGATGCGAGAATGCAGGTGCCTGCCCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAATGGCCTTGGTTATGGCCTCGCAATCCTGCTCATGGGAGTTCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACgcactgagga > 1:153095/4‑241 (MQ=255) ctAAAGCGTTCATCCAGCAGATGCGAGAATGCAGGCGCCTGACCGAAGATCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTA‑TACACGCTCGCCATATTGGGCGAAAAAGGCCTTGGTTATGGCCTCGCCACACTGCTCATGGGTGATCCCCGGCAGCAGCCCGGGGAGGGTGGTCActagggaccgaactgcggta > 2:543949/2‑230 (MQ=255) aaaGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AAt < 1:246815/249‑1 (MQ=255) aaaGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGc < 1:15805/251‑1 (MQ=255) gtacctCCAGCAGATGCTAGAAGGCCGGAGCCTGACCGAAGTTCCATTCTCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTc < 2:565100/246‑1 (MQ=255) cATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTcgcc < 2:240777/251‑1 (MQ=255) ccAGCAGATGCGAGAATGCCGGAGCCTGACCGCAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTATCCGGGGAGATGATTTCCGATTA‑TACACGCTATACATAATGGGCGCAAAAGGCCTTGGTTATGGCCTCGAATACCAGCTCATGGGTGATCCCCGGCAGTAGCTCGGTGAGACTGGTAACGCGGGAACGCACTG‑CATGAATGCCTTTCgccgcc > 2:28774/1‑251 (MQ=255) cagcagATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGc > 2:163640/1‑141 (MQ=255) cagcagATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGc < 1:163640/141‑1 (MQ=255) agcagATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTTGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCGCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAg < 2:371130/251‑1 (MQ=255) agATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCg > 2:361687/1‑162 (MQ=255) agATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCg < 1:361687/162‑1 (MQ=255) ggCGAGAATGCCGGGGCCTGACCGAAGTTCCTTTCCCAGCTTCTCTGACGGGCAAAGGTTTCGGCGAAGTTGGGCAAGGCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACCCGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGAGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCtt < 2:66009/250‑1 (MQ=255) agaATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGCTTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCtttttt > 2:486906/1‑250 (MQ=255) gCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCg < 1:615894/250‑1 (MQ=255) cGGAGCCTGACCGAAGGTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGAt < 1:87375/251‑1 (MQ=255) ggAGCCTGACCGACGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGACGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCACGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGA‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGAtt > 1:622251/1‑251 (MQ=255) ggAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGAtt > 1:625020/1‑251 (MQ=255) ccTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGCTGATTTACGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGATTATGGCCTAGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTAGCCGCCAGTTTCTTTTTATcagggatgagg > 2:640427/1‑241 (MQ=255) tGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCttg > 2:545186/1‑127 (MQ=255) tGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCttg < 1:545186/129‑3 (MQ=255) gACCGAAGTACCATTCCCAGCTACTGTGAGGGGCAAAGGTTTCGGGTAAGTTGGGAAAGTCTGGCGTTTTGT‑CCGGGAGATGATTTCCGCTTC‑TACACTCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTAGCCGGATTGAGATAg < 1:496789/250‑1 (MQ=255) aaGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTGCGGCGAAGTTGGGCAAGTCTGGCGTTTTGTCCGGGTAGATGATTTCCGCTGC‑GACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGGGAGGTTTGTCACGCGGGAACGCACTGAGGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTTAGATAGTTTGc < 2:622251/251‑1 (MQ=255) aGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAgg < 1:194969/176‑1 (MQ=255) aGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTC‑TACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAgg > 2:194969/1‑176 (MQ=255) | CCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTC‑CACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGT‑AATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGC > NC_000913/4623157‑4623631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |