| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,621,410 | T→C | I176I (ATT→ATC) | deoD → | purine nucleoside phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,621,410 | 0 | T | C | 98.6% | 201.1 / NA | 69 | I176I (ATT→ATC) | deoD | purine nucleoside phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (27/41); total (27/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGCATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCTAAAGCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATT‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAG > NC_000913/4621164‑4621648 | aGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCg > 1:450076/1‑251 (MQ=255)aGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCg < 1:115184/251‑1 (MQ=255) ttCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGc > 1:50365/1‑251 (MQ=255) gCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTAGCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCGCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTg < 1:435591/250‑1 (MQ=255) aCGTAAAACTGCGCGACGTCGTTAGCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATg < 1:49427/251‑1 (MQ=255) aaaaCTGCTCGACGTCTTTATCGGTATGGGTTCCTGCTCCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAg < 2:388744/251‑1 (MQ=255) aaaaCTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGaa < 1:335086/250‑1 (MQ=255) aaaCTGCGCGACTGCGGTATCGGTATGATTGCCTGCACCGATTCCATAGTTAACCTTATCCGTTTTAAAGACCATGGCTTTGCCGCTATCGCTGACTTGGACATGGTGCGTAACGCGGTAGACGCGGCGAAAGCACTGGGTGTTGATGCGCGCGTGGGTATCCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGc < 2:450076/251‑1 (MQ=255) gACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTAt > 1:122353/1‑251 (MQ=255) cgtcgtTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCt < 1:209549/251‑1 (MQ=255) cGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGCTGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGCATCTACGGCGTCg > 1:533805/1‑251 (MQ=255) cGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCg < 1:80385/251‑1 (MQ=255) cGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGAGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGACTGGGGTTTACGGCGTcc > 1:535636/1‑250 (MQ=255) ggTGCCTTCACCGATTCCAAAGTTAACCGTCTCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGa < 2:508958/251‑1 (MQ=255) gTGCCTGCACCGATTCCAAAGTTAACCGTTTCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCTGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGag < 2:171848/251‑2 (MQ=255) ccTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGttt > 1:43502/1‑251 (MQ=255) ccGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGCAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGcgcg > 1:300355/1‑251 (MQ=255) gATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGa > 1:44297/1‑239 (MQ=255) gATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGGTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGa < 2:44297/239‑1 (MQ=255) cAAAGTTAACCGTATCCATTTTAAAGATCATGACGTTGTCGCTATCGTTGACTTTGACAGGGTGAGTAACGCTGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCTGCTGATCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATTGAAGCTGCTGGTATCTACGGCGTAGATGCAGAGTTTGGCGCGAAAGccc < 2:472841/251‑1 (MQ=255) ccgtaTCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGCCGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGCCGTGATGGACAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCTTCGCTGCAGAGTTTGGCGCGAAAGCCCTGCCCATCt > 2:462255/5‑251 (MQ=255) tttAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTCACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAACGGCTGGTATTTACGGCGGTGCTGCAGAGTTTGGCCCGGAAGCCCTGACCctctcaccctggtc > 2:529508/1‑241 (MQ=255) tttAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGtgtc > 1:370306/1‑248 (MQ=255) aaaGTCCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTg < 1:86812/250‑1 (MQ=255) aaaGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGAAACGCGGTAGACGCGGATAAAGCACTGGGTGTTGATGCTCGCGTGGGAAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAACATACGGAATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTAAGGCGCCGCCGCAGAGTTTGGCGCGAAAGCCCTGTCCATCCGCACCgggtctgc > 1:471707/1‑244 (MQ=255) gACCATGACTTGGCCGCTTACGCCGCTTTCGACAAGATGCGTAACGCCGTCGACGCGGCTAAAGCACTGGGGGATGATGCTCGCGTGGGTAACCTGGTCACAGTTGTCCCGTTCTACTCTCCGGCCGGCGAAATGTTCGACGTGATGGAAAAATAGGGCATC‑CCCGGCGCGGAAATGGAAGCGGCTGGTACCCTCGGCGTCGCTTCAGAGTTTGGCTCGAAAGTCCTGACCATCTGCTCCGTTTCTGTCca < 2:35702/251‑1 (MQ=255) cATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATTCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGGGGAAATGGAAGCGGCTGGTATCTAAGGCGTCACTGCAGAGTTTGGGGCGAAAACCCTGAACATCTGCACCgagtcaggccacac > 2:599847/1‑238 (MQ=255) gCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTc < 1:127473/250‑1 (MQ=255) gtctttgACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCGACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCa < 2:50365/244‑1 (MQ=255) ggtgcgtaacgcgggagcctcggCTAGAGCATTGGGTTTTGATTCCCGCGTGTGTAACCTGTTCCCCGCTGACCGGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATTCGGCATC‑CTCTGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCTCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGGCCACTTCCGCACTCACGAGCAGACCACTGCCGCTGaa < 2:471707/228‑2 (MQ=255) gtaccgctgTAGACGCGGCTAAAGCACTGGTTGTTTATGCCCGCGTGGGTAACCTGTTCTCCGCTGACCTTTTTTCCTCCCCGTCCGGCGTAATTGTCGCCGTGATGGAAAAATCCGGCATC‑CCCGGCGTGGAAATGGAAGCGTCTGGTATCTCCGGTGTCGCTTCTGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCTCTTCCGCCCTCACGAGCAGACCACTTCCGCTGAGCGTCAg < 2:422671/243‑1 (MQ=255) gTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGCGCGTc > 1:229620/1‑249 (MQ=255) aacgtggtagactcggCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTTACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CGCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGa < 2:323818/235‑1 (MQ=255) aaCGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGAc > 1:587402/1‑251 (MQ=255) aCGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGAcc < 1:462255/251‑2 (MQ=255) gagacgcggCTAAAGCCCTGGGTGTGGATGCTCGCTGGGGTAGCCTGTTCTCCGCTGACCTGTTCTACTCTCCTGACGGCGAAATGGTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTGTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGTCGCTGAGCGTCAGACCACCTTc < 2:586373/243‑1 (MQ=255) aaGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGGGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCTCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATc < 1:293534/251‑1 (MQ=255) aGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATc > 1:12557/1‑250 (MQ=255) tttGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGGTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTAGCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCAc < 2:496489/249‑1 (MQ=255) gttGATGCTCGCGTGGGTAACCTGTGCTCCGCTGACCTTTTCTACTCGCCGGACGGCGAAATGTTCGGCGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTCCGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCAc < 2:370306/249‑1 (MQ=255) ggtGATGCGCGCGTGGGTATCCTGTTCTCCGCTGACCTGGTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGTGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGATCAGACCACTGCCGCTGTGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACt < 2:344305/249‑1 (MQ=255) gccgacctcgTGGGTAACCTGTTCTCCGCTGACCTGTTCTACCCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCAGC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCCCCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGa < 2:517503/243‑1 (MQ=255) gATGCTCGCGTGGGTAACCTGTGCGCCGCTGACCTGGTCTACTATCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CGCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTTGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCTCACTgg < 2:169865/250‑1 (MQ=255) attcTCGCGGGTGTATCCTGTTCTACTCTGACCTGTTATACTATCCGGACGGCGAAAGTTTCGACGTGCTGGAAAAATACGG‑ATTGCTCGGCTTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGACAGCCCCTACAATCTGCACCGTTTCTAACCACATCCGCACTCCCGAGCAGACCACTGCCGCTGAGCGTCAGACCAACTTAAACGACATGATCAAAATCGCGCTGGaa < 2:55135/248‑1 (MQ=255) cgTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTc < 1:273699/251‑1 (MQ=255) cgTGGGTAACCTGTTCTACGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGa > 1:517503/1‑243 (MQ=255) ggggggatccggTTCTCCGCTGACCTGGTCGACTCTCCGGACGGGGAAATGTGCGACGTGATGGAATAATACGGCAGC‑CTGGGCGGGGAAATGGAAGCGGCTGGGATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCGCCTGCAACGACATGATCAAAATCGCACTGGAAGCCGTTct < 2:325725/240‑1 (MQ=255) gTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAAt > 1:69877/1‑244 (MQ=255) gTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCCCCGGACGGCGAAATGGTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAAt < 2:69877/244‑1 (MQ=255) cccgatcttcctcTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAAGACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGGGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCACACTGGAATCCGTTCTGCTGGGc < 2:590111/239‑1 (MQ=255) tGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGAt > 1:555828/1‑251 (MQ=255) tcCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCACACTGGAATCCGTTCTGCTGGGc > 1:590111/1‑243 (MQ=255) cGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGt < 1:599847/251‑1 (MQ=255) gACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAAt < 2:576584/250‑1 (MQ=255) gtgcTACTCGCCTGACGGCGAAGTGTGTGACGTGATGGAAAAAGACGGCATC‑CTCGGGGTGGTTATGGAAGCGGCTGTTATCTACGGCGTCGCTGCAGTGTGTGGCGCGAAAGCCCCGACCATCTGCACCGGGTCTGACCACATCCGCGCTCACGGGCAGACCACTGCCGCTGAGCTTCAGAGCACCTTCAACGACATGATGGAAAGCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTgtgt < 2:237976/247‑1 (MQ=255) ggtCTACTCTCCGGACAGCGAAATGTTCGACGTGGTGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTCTCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGACTGACCACATCCGCACTAACGAGCAGACCACTGCCGCTGAGCGTCAGAACACCTTCAACGACATGATCAAAATCGCACTGGCATCCGTTCCGCCGGGCGATaaatggaaattatgtt > 2:489428/3‑238 (MQ=255) tCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGttt < 1:394583/250‑1 (MQ=255) ccGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTgaa > 1:447675/1‑247 (MQ=255) cggacgcagAAATGTTCTACGTGCTGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACTGCCTCGCTGCCGAGTTTGGAGCGCAAGCCCTGACCATCTGCACCGTGTCTGACCACCTCCGCACTCAAGCGCAGACCACTACCGATGAGCGCCAGACCACCTTCCACGACATGATCAAAATCGCAATGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTATGTTTCGCtcaaagg > 2:429925/9‑245 (MQ=255) ggACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTggg > 1:601968/1‑219 (MQ=255) ggACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACAGCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCTCACTGGAATCCGTTCTGCTggg < 2:601968/219‑1 (MQ=255) aCGGCGAAATGTTCGACGTTATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGATTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATTAAAATCGCACGGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGa < 2:45817/251‑1 (MQ=255) cGAAATGTTCGACGTGATGGCAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACGTGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATACAGAGTAATTGTGTTTCGCTGAAAGGCGaaagt > 2:597808/1‑247 (MQ=255) cGACGTGATGGAAAAATACGGCATC‑CTCGGCGGGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGGTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACGGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAAACCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGaacgtcgtgttaag < 2:5120/251‑14 (MQ=255) tGATGGAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGCTAAAGAGTAATTGTGTTTCGCTGAAAGGCGaacgtcgtgttaagccgg > 1:298655/1‑233 (MQ=255) ggAAAAATACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGcag < 2:587402/251‑3 (MQ=255) aaaaTACGGCATC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGc < 1:61886/216‑1 (MQ=255) aaaaTACGGCAGC‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTGTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGgag < 1:342470/251‑1 (MQ=255) aaaaTAAGCCATC‑CTCGGCGTGGAAATTGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGAGCGATAGCCCTGACCATCTGCACCGTGTCTGACCACAGCAGGAATCACGAGCAGACCACTGCCGCTGGGCGTCAGACCACCTTCACCGACATGATCAAAATCGCACTGGAATCCGATCTGCTGGGCGTTAAAGAGTATTTGTGTTTCGc > 2:61886/1‑216 (MQ=255) | AGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGCATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCTAAAGCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATT‑CTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAG > NC_000913/4621164‑4621648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |