Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,652	C→T	C350C (TGC→TGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,652	0	C	T	96.8%	181.3 / ‑2.6	64	C350C (TGC→TGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (20/41);  total (21/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

ACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAAT  >  NC_000913/4620410‑4620884                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         aCCAAGAAATTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGAc                                                                                                                                                                                                                                  <  1:160310/251‑1 (MQ=255)                 caaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAc                                                                                                                                                                                                                  <  2:345111/249‑1 (MQ=255)                  aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGTTACTGAcc                                                                                                                                                                                                                 >  1:540559/2‑251 (MQ=255)                    cggtcctggaagttcTGTTCGACGCCACCATCAAAGAGATGAAAAAAGCG‑GGTGATAACATCAACGTCTTCACCAACTTCGTTGCCTTCGCCTCTTCCTGGGGCCACCTTCGCGACGTCGCCGTTTAGGTTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATTACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcctc                                                                                                                                                                                                               <  2:537280/237‑3 (MQ=255)                    cTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGCGATGAACGAAGCG‑GGTGATAACACCATCGGCTTCACCAACTTCGTTGACTTCGACTCTTCATGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGATCTGGAGCGGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGGGATcagaacagcacagggactgacccc                                                                                                                                                                                                               >  2:310731/1‑228 (MQ=255)                       gCCTGGACGCGCTGTTGGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACAcg                                                                                                                                                                                                            <  1:611779/251‑1 (MQ=255)                        ccTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGGCCtagatcggacgagcgtcgggtt                                                                                                                                                                                                           >  2:209451/1‑230 (MQ=255)                        ccTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCt                                                                                                                                                                                                                                <  1:209451/230‑1 (MQ=255)                              cgcgCTGTTTGACACCACAATCAAAGAGATGAAAGAAGAG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGACTGCAGGAGCTGATGTCTCTGATGCGCGATGACGACATCATGCTCCTCAATGCAGACAACGGTTGTGATCCGACCTGGACCGGCAATGCCCACACGCGTAAac                                                                                                                                                                                                     >  2:619381/1‑251 (MQ=255)                                 gcggattGACGCCCCCAGCAAAGAGATGAACGCAGCG‑GGTGATAACACCATCGTATTAACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCCACGTCCCCGGTTCGGCGGCAGGTCTGGAGCTGTTCGCCCGCCATCTACCGGAGCTGCTGTCTCTGCTGCCCGATTACGACATACTGATCCTCTCTGCTGCCCACGGTTGTGATCCGACCTCGACAGGTAATAACCACACACGttcacac                                                                                                                                                                                                   >  2:315180/6‑244 (MQ=255)                                      ttGACGCCCCCATCAAAGAGATGAAGGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCTGCTCTTCCTGGGGCCACCGTCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGGCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTcc                                                                                                                                                                                              <  2:591204/250‑1 (MQ=255)                                      ttGACGCCACCAACAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACGAACTTCGTTGACTTCGACCCTGCCTGGGGCCACCGTCGCGATGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTGCTACCGCCGTCTGCCGGAGCTGATGTCTCTGCGGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATTCGACCTGGACCGGTACTGACCACACGCGTGAACTCATTcc                                                                                                                                                                                              <  2:465348/250‑1 (MQ=255)                                        gaagCCACCATCAAAGAGATGAAAGAAGTGTGGT‑ATAACCACATCGTCTTCACCAACTTCGTTGACTCCGCCGCTTCCTTGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGAGGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGt                                                                                                                                                                                           <  2:539849/248‑1 (MQ=255)                                          ccccaccaTCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCATCACCAACTTCGTTGACTTCGTCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTTTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCGCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGGTCCGACCTGGCACGGTACTGACCACGCGCGTGAACACATTCCTGTAc                                                                                                                                                                                         <  2:478383/249‑1 (MQ=255)                                          cGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTAc                                                                                                                                                                                         >  1:159945/1‑251 (MQ=255)                                             caccaTCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTgg                                                                                                                                                                                      <  1:261468/251‑1 (MQ=255)                                              accaTCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                                                                                                     <  2:519052/251‑1 (MQ=255)                                                   cAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacg                                                                                                                                                                                <  1:558897/251‑6 (MQ=255)                                                     aaGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacggc                                                                                                                                                                              <  1:457009/251‑8 (MQ=255)                                                       gagaTGAAAGAAGCG‑GGAGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGccc                                                                                                                                                                            >  2:180261/1‑251 (MQ=255)                                                            gAAAGAAGCG‑GGTGATAACACCAGCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACTGTCGCGACGCCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACAGACGTCTGCCGGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTAACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAg                                                                                                                                                                       <  1:657202/251‑1 (MQ=255)                                                             aaaGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGCAAGt                                                                                                                                                                      >  1:158440/1‑251 (MQ=255)                                                                  agCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAc                                                                                                                                                                 <  1:293571/251‑1 (MQ=255)                                                                            tAACACCATCGTCTTCACCCACTTCGTTTACTTCGGCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGCCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCATGATCCTCACTGCTGACCACGGTTGTGATCTGACCTGGACCGGTACTGAACACACGCGTGAACACATTCAGGTACTGGTATACGGCCCGGAAGTAACCCCGGGCTCAc                                                                                                                                                        >  2:392731/1‑251 (MQ=255)                                                                             aaCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACt                                                                                                                                                       <  2:315824/251‑1 (MQ=255)                                                                                      gTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCaccg                                                                                                                                              <  1:167342/251‑4 (MQ=255)                                                                                           cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGACCTGGTTTACGGCCCGAAAGTaaaccc                                                                                                                                                                >  2:146055/1‑225 (MQ=255)                                                                                           cACCAACTTCGGTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAcc                                                                                                                                                                <  1:146055/228‑1 (MQ=255)                                                                                            aCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAAc                                                                                                                                        >  1:511092/1‑251 (MQ=255)                                                                                                actgcGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                                                                                                    <  1:292990/247‑1 (MQ=255)                                                                                                   tatttGACTTGGACACGTCCTGGGTCCACCGTCGCGACGTCGCCGGTTAGGCCGCGGGTCTGGGGCTGGTCGTCCGCCGTCTGCCTGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTGACTGCTGACCACTGTTGTGATCCGACCGGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcg                                                                                                                                   <  2:648744/246‑1 (MQ=255)                                                                                                   tCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgc                                                                                                                                  <  1:371858/250‑1 (MQ=255)                                                                                                   tCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcg                                                                                                                                   <  1:240837/249‑1 (MQ=255)                                                                                                    cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgcg                                                                                                                                 <  1:574922/250‑1 (MQ=255)                                                                                                     gTTGACTTCGCCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGa                                                                                                                               <  1:245407/251‑1 (MQ=255)                                                                                                       tGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGat                                                                                                                              >  1:291151/1‑250 (MQ=255)                                                                                                          cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAATACCGGGCTCACTGGGTCCCCGGGAAACCTTCCCGGattttt                                                                                                                          >  2:575972/1‑247 (MQ=255)                                                                                                             cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGatattggt                                                                                                                        <  1:507597/250‑5 (MQ=255)                                                                                                             cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGcc                                                                                                                       <  1:290173/251‑1 (MQ=255)                                                                                                             cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATACTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGcc                                                                                                                       <  1:332042/251‑1 (MQ=255)                                                                                                                  ctTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct                                                                                                                  >  1:652716/1‑251 (MQ=255)                                                                                                                     ccgggggCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACTCATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                                                                                               <  2:342506/248‑1 (MQ=255)                                                                                                                             cACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGATCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAata                                                                                                       <  2:386476/251‑1 (MQ=255)                                                                                                                              aCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGCCCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCCGCCTCTGGCAACatat                                                                                                      >  2:86529/1‑251 (MQ=255)                                                                                                                                              gggtgattctgctgggcgggagcTGTTCCACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCTATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAATCCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGata                                                                                      <  2:548329/229‑1 (MQ=255)                                                                                                                                                        gcgGGTCTGGAGCTTTTCGACCGCCGTCTGCCGTATCTGATGTCTCTGCTGCGCGATGACGATGTCCTGATCCTCACTGCTTACCACGGTTGTGATCCGTCCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGGGAAACCTTCGCGGATATTGGTCAGACGCGGGCAAAATATTGTGGTACCGCTGATATGGAATATgg                                                                            <  2:329055/251‑1 (MQ=255)                                                                                                                                                            gTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGACAAGGAATATGGCaa                                                                         >  2:170604/1‑250 (MQ=255)                                                                                                                                                                ggagcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATACTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGATTACGGCCCGAAAGAAAAACCGGGATCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAACTATTTTGGTACTTCTGATATGGAATATGGCCAAGcc                                                                     >  1:654227/5‑250 (MQ=255)                                                                                                                                                                  gggctgtCGACCGCCGTCTGCCGCATCTGATGCCTCTGCTGCGCGATGACGACATCTTGATCCTCACTGCTGACCACGGGTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTTATATGGAATATGGCAAAGCCATg                                                                  <  2:540559/245‑1 (MQ=255)                                                                                                                                                                   gcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGt                                                                 <  1:555932/250‑1 (MQ=255)                                                                                                                                                                      gTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTc                                                               <  1:170604/250‑1 (MQ=255)                                                                                                                                                                                     gCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcg                                                 <  1:60062‑M1/249‑11 (MQ=255)                                                                                                                                                                                              gATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccc                                      >  1:144726‑M1/1‑230 (MQ=255)                                                                                                                                                                                                         tgcGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctccccccggcc                           >  1:233616‑M1/1‑219 (MQ=255)                                                                                                                                                                                                                         ccttatcctCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTCACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggaga           <  2:159945‑M1/247‑49 (MQ=255)                                                                                                                                                                                                                         ccTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggag            <  1:404521‑M1/250‑48 (MQ=255)                                                                                                                                                                                                                            gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTTAACACATTCCGGTACTGGTGTACGGCCCGAATGTAAAACCGGGCTCACTGGGGCACCGTGAAATCTTcgc                                                                                                                                  <  2:307060/129‑1 (MQ=255)                                                                                                                                                                                                                            gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAACGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgc                                                                                                                                  >  1:307060/1‑129 (MQ=255)                                                                                                                                                                                                                            gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagg         <  1:86529‑M1/250‑51 (MQ=255)                                                                                                                                                                                                                            gATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACGGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagag          <  2:562635‑M1/249‑50 (MQ=255)                                                                                                                                                                                                                             aTCCTCACTGCTGACCACTGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggt       <  2:652716‑M1/251‑53 (MQ=255)                                                                                                                                                                                                                                  cactgctgaCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaa  <  1:180261‑M1/247‑58 (MQ=255)                                                                                                                                                                                                                                                gTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGa                                                                                                                    <  1:520130/123‑1 (MQ=255)                                                                                                                                                                                                                                                gTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGa                                                                                                                    >  2:520130/1‑123 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         ACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCG‑GGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAAT  >  NC_000913/4620410‑4620884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑